FastQCFastQC Report
Sat 14 Jan 2017
SRR2935272.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935272.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442564
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGC55941.2639979754340616No Hit
CTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGCT43650.9862980269520341Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGC32390.7318715485217957No Hit
GCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGC26850.6066919134859591No Hit
TCCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTG11130.25148905017127465No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGC11040.2494554459919921No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCG9320.2105910105657035No Hit
CGCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTG9080.2051680660876167No Hit
GAATCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTC8660.19567791325096484No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCC8250.18641371643423324No Hit
TTCCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCT8030.181442683995987No Hit
TGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGCTT7790.17601973951790023Illumina Single End Adapter 2 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTG7330.16562576260156725No Hit
TGCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTG6610.14935692916730686No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTC5980.13512169991232909No Hit
CCTGACTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGC5450.12314603085655407No Hit
TTCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTG5240.11840095443822814No Hit
CTGTATCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGCT4480.10122829692428666Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTACG502.1827873E-1145.0000041
CGTGGGT502.1827873E-1145.0000044
GGTACAA253.886904E-545.0000048
GGTAATC302.1624655E-645.0000048
AAGACGC253.886904E-545.00000439
CCTACGG302.1624655E-645.00000416
TTACTTG302.1624655E-645.0000041
TTGTACG600.045.0000041
GGATACC302.1624655E-645.0000048
GGATACA302.1624655E-645.0000048
ATACTAT302.1624655E-645.00000445
AGACACA253.886904E-545.00000433
GGACGAT302.1624655E-645.0000048
GCGGCTC302.1624655E-645.0000049
TTACGTG253.886904E-545.0000041
GGTTGAC253.886904E-545.00000410
ACCGTTA302.1624655E-645.00000420
GACGATT302.1624655E-645.0000049
ACTTTAC253.886904E-545.00000427
TGGTGCG253.886904E-545.0000041
Produced by FastQC (version 0.11.5)