##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935272.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	442564
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15194412559539	33.0	31.0	34.0	30.0	34.0
2	32.30843448631158	34.0	31.0	34.0	30.0	34.0
3	32.36024168255891	34.0	31.0	34.0	30.0	34.0
4	35.88126237109209	37.0	35.0	37.0	35.0	37.0
5	32.255316745148725	37.0	35.0	37.0	0.0	37.0
6	33.69185247783371	37.0	35.0	37.0	17.0	37.0
7	35.26476170678139	37.0	35.0	37.0	32.0	37.0
8	35.742050867219206	37.0	35.0	37.0	35.0	37.0
9	37.745896638678246	39.0	37.0	39.0	35.0	39.0
10	37.47548829095905	39.0	37.0	39.0	35.0	39.0
11	37.36627696785098	39.0	37.0	39.0	35.0	39.0
12	37.087772615938036	39.0	35.0	39.0	34.0	39.0
13	36.89100559467105	39.0	35.0	39.0	33.0	39.0
14	37.92741162860061	40.0	36.0	41.0	33.0	41.0
15	38.04772417096736	40.0	36.0	41.0	33.0	41.0
16	38.17152321472149	40.0	37.0	41.0	34.0	41.0
17	38.19890456521543	40.0	37.0	41.0	34.0	41.0
18	38.21539483554921	40.0	37.0	41.0	34.0	41.0
19	38.24045109859817	40.0	37.0	41.0	34.0	41.0
20	38.13819470178324	40.0	36.0	41.0	34.0	41.0
21	38.06404271472601	40.0	36.0	41.0	34.0	41.0
22	38.02528899774948	40.0	36.0	41.0	34.0	41.0
23	38.019506783199716	40.0	36.0	41.0	34.0	41.0
24	37.99528203830406	40.0	36.0	41.0	34.0	41.0
25	37.90737384875408	40.0	36.0	41.0	34.0	41.0
26	37.79511663849748	39.0	36.0	41.0	34.0	41.0
27	37.65994748782097	39.0	35.0	41.0	33.0	41.0
28	37.66896991169639	39.0	36.0	41.0	33.0	41.0
29	37.653417358845275	39.0	36.0	41.0	33.0	41.0
30	37.662627326217226	39.0	36.0	41.0	33.0	41.0
31	37.44629477318535	39.0	35.0	41.0	33.0	41.0
32	37.43694245352085	39.0	35.0	41.0	33.0	41.0
33	37.405333465894195	39.0	35.0	41.0	33.0	41.0
34	37.33032284596126	39.0	35.0	41.0	33.0	41.0
35	37.27046483672418	39.0	35.0	41.0	33.0	41.0
36	37.19798492421435	39.0	35.0	41.0	32.0	41.0
37	37.10311502969062	39.0	35.0	41.0	32.0	41.0
38	37.188225883714	39.0	35.0	41.0	32.0	41.0
39	37.29753888703103	39.0	35.0	41.0	33.0	41.0
40	37.22434043437785	39.0	35.0	41.0	33.0	41.0
41	37.181178315452684	39.0	35.0	41.0	32.0	41.0
42	37.136133982881574	39.0	35.0	41.0	32.0	41.0
43	36.97439918294303	39.0	35.0	40.0	32.0	41.0
44	36.88358971809727	39.0	35.0	40.0	32.0	41.0
45	36.95758805506096	39.0	35.0	40.0	32.0	41.0
46	36.8976803354995	39.0	35.0	40.0	32.0	41.0
47	36.84856201588923	39.0	35.0	40.0	32.0	41.0
48	36.87040518433492	39.0	35.0	40.0	32.0	41.0
49	36.89414412378775	39.0	35.0	40.0	32.0	41.0
50	36.78185753924856	39.0	35.0	40.0	32.0	41.0
51	36.17966215055902	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	5.0
11	2.0
12	4.0
13	6.0
14	9.0
15	12.0
16	20.0
17	22.0
18	42.0
19	78.0
20	139.0
21	263.0
22	367.0
23	611.0
24	924.0
25	1367.0
26	2028.0
27	2533.0
28	3362.0
29	4412.0
30	6008.0
31	8185.0
32	11413.0
33	17714.0
34	29425.0
35	46138.0
36	37613.0
37	58034.0
38	95880.0
39	115852.0
40	93.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.02386999394438	4.97939281098327	52.34994260717094	18.64679458790141
2	19.85226995417612	5.9623014976365	51.73602010104753	22.44940844713985
3	17.78183494364657	5.649126454026988	54.08189549985991	22.487143102466536
4	18.593920879240063	4.246843394401713	52.939009951103124	24.220225775255106
5	15.65558879619671	14.943149465388055	46.71595520647861	22.685306531936625
6	19.46950045643116	6.634294700879422	53.5716868068799	20.32451803580951
7	77.75688939904737	2.081280899485724	13.63486411004962	6.526965591417287
8	78.38934029880424	3.268001916107049	10.050749722074096	8.291908063014615
9	72.92256035285291	5.464746341772037	13.189956706826583	8.422736598548457
10	37.333809347348634	25.83016241718712	19.729123923319566	17.10690431214468
11	28.6261422076807	26.153279525673124	26.13384730795998	19.0867309586862
12	27.37344203324265	20.65712529713217	31.838558942887357	20.13087372673783
13	23.105810685008272	27.388129174537468	31.044323532867562	18.461736607586698
14	17.19028208349527	32.428756066919135	29.621252519409623	20.759709330175973
15	13.366428358384324	26.39121121464918	39.881237515929904	20.361122911036595
16	14.966196979419927	24.72094431539845	35.80363518044848	24.509223524733144
17	15.806301461483537	24.092786580020064	29.343552570927596	30.757359387568805
18	17.620050433383646	22.98740973057004	33.98649686824957	25.40604296779675
19	20.63791903543894	24.67394546325503	31.677000388644355	23.01113511266167
20	23.68154662376515	25.47360381775291	31.69711047441726	19.14773908406468
21	21.527507885865095	28.17897524425846	29.440939615513233	20.85257725436321
22	17.474760712574906	26.076680434920146	28.390018166864	28.05854068564095
23	17.91718259957882	28.14643757738994	26.79680227040609	27.139577552625155
24	20.755416165797488	24.460417024430363	30.052602561437443	24.731564248334703
25	16.929980748547102	27.67757883605535	27.588326208186835	27.804114207210706
26	15.821440514818196	32.87773067850074	27.87687204562504	23.42395676105603
27	16.94602362596144	33.73568568613805	27.62108983107528	21.697200856825226
28	13.597355410742853	30.615684963078788	33.57322330781536	22.213736318362994
29	15.516851799965655	28.578013575437677	32.7347005178912	23.17043410670547
30	20.737339684203867	30.06051102213465	27.58290326370875	21.61924602995273
31	21.880451188980576	31.012011822018966	23.320242947912618	23.787294041087844
32	23.804466698601782	30.206026698963313	23.958342748167496	22.031163854267405
33	25.301425330573657	28.086559232111064	23.348713406422576	23.263302030892707
34	19.90039858641914	27.095742084760623	26.299247114541625	26.70461221427861
35	20.358185482777632	27.341808190453808	26.360707151959943	25.939299174808617
36	24.766361475402427	30.06480418651314	25.04044612756573	20.128388210518704
37	19.286024168255892	32.125297132166196	28.90000090382408	19.688677795753833
38	21.04328413517593	30.285789173995177	26.384658490071494	22.286268200757405
39	24.732694028434306	23.125468858741336	29.463987129545107	22.677849983279255
40	26.974403702063434	22.36354515957014	26.246599361900202	24.41545177646623
41	21.162137001653996	26.359577371860343	22.919171012554116	29.55911461393154
42	26.076906390940067	25.884391861967988	24.010312632749162	24.028389114342787
43	25.669507687023795	25.71808823130666	25.881906345748863	22.73049773592068
44	20.193237588235828	26.738505617266657	27.599398053162933	25.46885874133459
45	20.549118319610272	25.43699894252583	25.829484549127358	28.18439818873654
46	24.387207273976195	25.32876600898401	27.746043510091194	22.5379832069486
47	19.273822543180195	26.01567230954167	30.604839074122616	24.105666073155522
48	21.50016720745474	23.827740168653573	29.935105431078895	24.73698719281279
49	23.639970716099818	19.974512160952994	32.81062174058441	23.574895382362776
50	22.53075261431115	20.711354741913034	29.116918682947553	27.640973960828262
51	20.592275919415044	20.929176345116186	25.374861037047747	33.10368669842102
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	170.0
1	260.0
2	350.0
3	557.5
4	765.0
5	624.5
6	484.0
7	554.0
8	624.0
9	790.5
10	957.0
11	1239.0
12	1521.0
13	1520.0
14	1519.0
15	1537.5
16	1556.0
17	1394.5
18	1233.0
19	1227.0
20	1221.0
21	1421.5
22	1622.0
23	1852.5
24	2083.0
25	2116.0
26	2444.0
27	2739.0
28	3368.0
29	3997.0
30	4462.0
31	4927.0
32	5778.0
33	6629.0
34	7493.0
35	8357.0
36	9254.0
37	10151.0
38	12201.0
39	14251.0
40	17329.5
41	20408.0
42	25831.5
43	31255.0
44	36009.0
45	40763.0
46	49669.5
47	58576.0
48	63740.0
49	68904.0
50	64730.5
51	60557.0
52	46735.0
53	32913.0
54	26351.5
55	19790.0
56	15704.0
57	11618.0
58	10803.5
59	9989.0
60	9108.0
61	8227.0
62	6709.0
63	5191.0
64	4037.5
65	2884.0
66	2258.0
67	1632.0
68	1313.5
69	995.0
70	856.5
71	718.0
72	517.0
73	316.0
74	262.5
75	202.5
76	196.0
77	131.5
78	67.0
79	51.5
80	36.0
81	24.5
82	13.0
83	7.0
84	1.0
85	1.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	442564.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.48379571848955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.21757231145016	19.88079151598067
2	13.570309102979007	8.273490610639422
3	6.432361490870055	5.882483810254848
4	3.5537282749506924	4.333245070904687
5	2.2680393852055882	3.4569224650047885
6	1.4992404347113737	2.7421523486784594
7	0.9534201250490525	2.0344705028124457
8	0.7869253791574555	1.919077800314465
9	0.617071602753729	1.6929616213823047
>10	4.622758552045959	30.59965451562053
>50	0.35576680702218333	7.047287765940985
>100	0.11015373941272621	6.176274671274121
>500	0.008187102253648569	1.8545613534271792
>1k	0.0037214101152948046	2.8374289559530586
>5k	7.442820230589609E-4	1.2691969918120432
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGC	5594	1.2639979754340616	No Hit
CTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGCT	4365	0.9862980269520341	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGC	3239	0.7318715485217957	No Hit
GCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGC	2685	0.6066919134859591	No Hit
TCCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTG	1113	0.25148905017127465	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGC	1104	0.2494554459919921	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCG	932	0.2105910105657035	No Hit
CGCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTG	908	0.2051680660876167	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTC	866	0.19567791325096484	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCC	825	0.18641371643423324	No Hit
TTCCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCT	803	0.181442683995987	No Hit
TGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGCTT	779	0.17601973951790023	Illumina Single End Adapter 2 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTG	733	0.16562576260156725	No Hit
TGCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTG	661	0.14935692916730686	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTC	598	0.13512169991232909	No Hit
CCTGACTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGC	545	0.12314603085655407	No Hit
TTCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTG	524	0.11840095443822814	No Hit
CTGTATCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGCT	448	0.10122829692428666	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.061315425565568	0.0
2	0.0	0.0	0.0	3.9573937328838316	0.0
3	0.0	0.0	0.0	5.364195912907512	0.0
4	0.0	0.0	0.0	6.832458130349509	0.0
5	0.0	0.0	0.0	9.43795699605029	0.0
6	0.0	0.0	0.0	10.840240055675563	0.0
7	0.0	0.0	0.0	12.20546632803391	0.0
8	0.0	0.0	0.0	14.35498594553556	0.0
9	0.0	0.0	0.0	15.311909689897957	0.0
10	0.0	0.0	0.0	17.44719407814463	0.0
11	0.0	0.0	0.0	21.12349852224763	0.0
12	2.259560199202827E-4	0.0	0.0	24.674397375294873	0.0
13	2.259560199202827E-4	0.0	0.0	25.67131533518316	0.0
14	2.259560199202827E-4	0.0	0.0	25.985620158892274	0.0
15	2.259560199202827E-4	0.0	0.0	26.508482388987808	0.0
16	2.259560199202827E-4	0.0	0.0	27.85427644363301	0.0
17	2.259560199202827E-4	0.0	0.0	29.86754458112273	0.0
18	2.259560199202827E-4	0.0	0.0	32.38785802731356	0.0
19	2.259560199202827E-4	0.0	0.0	33.74833922325359	0.0
20	2.259560199202827E-4	0.0	0.0	35.137064921683645	0.0
21	2.259560199202827E-4	0.0	0.0	36.935448884229174	0.0
22	0.0011297800996014136	0.0	0.0	38.452969514013795	0.0
23	0.0011297800996014136	0.0	0.0	39.93275548847173	0.0
24	0.0011297800996014136	0.0	0.0	41.050108006977524	0.0
25	0.0011297800996014136	0.0	0.0	41.94014876944352	0.0
26	0.0011297800996014136	0.0	0.0	42.78251281170633	0.0
27	0.0011297800996014136	0.0	0.0	43.56612828878987	0.0
28	0.0011297800996014136	0.0	0.0	44.41301145145109	0.0
29	0.0011297800996014136	0.0	0.0	45.233457759781636	0.0
30	0.0011297800996014136	0.0	0.0	46.23602462016793	0.0
31	0.0011297800996014136	0.0	0.0	47.10324382462198	0.0
32	0.0011297800996014136	0.0	0.0	47.907873211558105	0.0
33	0.0011297800996014136	0.0	0.0	48.69849332525917	0.0
34	0.0011297800996014136	0.0	0.0	49.4685514411475	0.0
35	0.0011297800996014136	0.0	0.0	50.36785640043022	0.0
36	0.0011297800996014136	0.0	0.0	51.07374300666118	0.0
37	0.0011297800996014136	0.0	0.0	51.81329705986027	0.0
38	0.0011297800996014136	0.0	0.0	52.5232508744498	0.0
39	0.0011297800996014136	0.0	0.0	53.24585822615486	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCTACG	50	2.1827873E-11	45.000004	1
CGTGGGT	50	2.1827873E-11	45.000004	4
GGTACAA	25	3.886904E-5	45.000004	8
GGTAATC	30	2.1624655E-6	45.000004	8
AAGACGC	25	3.886904E-5	45.000004	39
CCTACGG	30	2.1624655E-6	45.000004	16
TTACTTG	30	2.1624655E-6	45.000004	1
TTGTACG	60	0.0	45.000004	1
GGATACC	30	2.1624655E-6	45.000004	8
GGATACA	30	2.1624655E-6	45.000004	8
ATACTAT	30	2.1624655E-6	45.000004	45
AGACACA	25	3.886904E-5	45.000004	33
GGACGAT	30	2.1624655E-6	45.000004	8
GCGGCTC	30	2.1624655E-6	45.000004	9
TTACGTG	25	3.886904E-5	45.000004	1
GGTTGAC	25	3.886904E-5	45.000004	10
ACCGTTA	30	2.1624655E-6	45.000004	20
GACGATT	30	2.1624655E-6	45.000004	9
ACTTTAC	25	3.886904E-5	45.000004	27
TGGTGCG	25	3.886904E-5	45.000004	1
>>END_MODULE