FastQCFastQC Report
Sat 14 Jan 2017
SRR2935270.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935270.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2814254
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC100590.3574304238352331No Hit
CTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGCT66270.23547981099076346Illumina Single End Adapter 1 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG49010.1741491706150191No Hit
TCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC46080.16373788577718998No Hit
GCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC42390.15062606289268843No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATG406.8212103E-945.0000041
CGACGGA253.8922513E-545.020
CGCGCAT351.2127748E-745.015
GCGATCG253.8922513E-545.09
AATCGTC253.8922513E-545.038
TTTACGG13150.042.262362
ACCGGTA750.042.041
CGTATGG3000.042.02
TACGGGA14050.041.7971534
TTACGGG22100.041.436653
TATGGGA43450.041.3751454
TTATGGG61450.041.3018723
TCTACGG4800.041.2499962
TCTTACG3250.040.1538471
TATGGGC15150.039.6534654
TTTATGG36900.039.451222
CTACGGG10100.039.2079163
ACGGGAT8650.038.757235
TGGGCGA32750.038.748096
TTTGGGC49500.038.7272724
Produced by FastQC (version 0.11.5)