SRR2935269.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935269.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3206233
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGCT	10621	0.33126101565294847	TruSeq Adapter, Index 27 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	8961	0.2794868619966172	TruSeq Adapter, Index 16 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	8561	0.2670111623203928	TruSeq Adapter, Index 16 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	6941	0.21648457863168397	TruSeq Adapter, Index 16 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6225	0.1941530762112423	No Hit
CGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	3845	0.1199226631377071	TruSeq Adapter, Index 27 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	3770	0.11758346944841501	TruSeq Adapter, Index 27 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTCGA	20	7.0351793E-4	45.0	21
CGAATCG	20	7.0351793E-4	45.0	35
CGTAACG	20	7.0351793E-4	45.0	28
CGATCCA	20	7.0351793E-4	45.0	17
CATTTCG	60	0.0	44.999996	1
CGTTATT	690	0.0	43.695652	1
TTACGGG	1925	0.0	41.727272	3
TATGGGA	4455	0.0	41.56566	4
TGTTACG	195	0.0	41.53846	1
TACGGGA	1365	0.0	41.20879	4
TACGGGT	455	0.0	41.043957	4
TTTACGG	1175	0.0	40.787235	2
CGTTTTT	5000	0.0	40.725002	1
TTATGGG	7185	0.0	40.678497	3
CCGTTTA	100	0.0	40.500004	13
CTACGGG	1065	0.0	39.718307	3
TTTATGG	4045	0.0	39.381954	2
GCGCGAC	740	0.0	39.222973	9
ACGGGAT	890	0.0	38.932583	5
TGGGCGA	4620	0.0	38.863636	6