FastQCFastQC Report
Sat 14 Jan 2017
SRR2935268.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935268.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649536
Sequences flagged as poor quality0
Sequence length51
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG9580.1474899004828062No Hit
CCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTCTGC9510.14641220809932012No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG8780.13517341610010838No Hit
CTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTCTGCT7140.1099246231155779Illumina Single End Adapter 2 (95% over 21bp)
TTTTTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA6510.10022539166420338No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT1900.045.0000044
TTACGGG10250.045.0000043
TTGATCG253.8889262E-545.02
AGATACG207.031019E-445.07
CTATCGG351.2108285E-745.03
TAACGAA351.2108285E-745.019
ATCGTTC207.031019E-445.022
CCGTATG406.8066583E-945.01
TACGAAT850.045.012
TAGGCGT700.045.04
CCGATAA302.164039E-644.99999615
ATTCCGA302.164039E-644.99999612
TATTACG302.164039E-644.9999961
CGTAAGG302.164039E-644.9999962
CGATAAC302.164039E-644.99999616
ACGAACG302.164039E-644.99999621
TACGGGG9050.044.5027584
TTCACGG2800.044.1964262
TTTACGG6300.043.571432
CTACGGG4650.043.548393
Produced by FastQC (version 0.11.5)