##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935268.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	649536
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.91253448615627	34.0	31.0	34.0	31.0	34.0
2	33.045524805399545	34.0	33.0	34.0	31.0	34.0
3	33.09576528475712	34.0	33.0	34.0	31.0	34.0
4	36.368830980884816	37.0	37.0	37.0	35.0	37.0
5	23.450664782244555	37.0	0.0	37.0	0.0	37.0
6	29.409811003547148	37.0	17.0	37.0	17.0	37.0
7	34.43859616711006	37.0	32.0	37.0	32.0	37.0
8	35.703440609912306	37.0	35.0	37.0	35.0	37.0
9	38.12695832101685	39.0	39.0	39.0	37.0	39.0
10	38.074411579958614	39.0	38.0	39.0	37.0	39.0
11	38.01433638782146	39.0	38.0	39.0	35.0	39.0
12	37.565486747462806	39.0	37.0	39.0	35.0	39.0
13	37.345826867179035	39.0	37.0	39.0	35.0	39.0
14	38.028363323972805	40.0	38.0	41.0	33.0	41.0
15	38.31255388461918	40.0	38.0	41.0	33.0	41.0
16	38.61818128633363	40.0	38.0	41.0	35.0	41.0
17	38.72319163218051	40.0	38.0	41.0	35.0	41.0
18	38.73772816287319	40.0	37.0	41.0	35.0	41.0
19	38.751307086905115	40.0	37.0	41.0	35.0	41.0
20	38.636692962360826	40.0	37.0	41.0	35.0	41.0
21	38.59942635973987	40.0	37.0	41.0	35.0	41.0
22	38.65433478667849	40.0	37.0	41.0	35.0	41.0
23	38.64185202975663	40.0	37.0	41.0	35.0	41.0
24	38.60295657207607	40.0	36.0	41.0	35.0	41.0
25	38.53858908513154	40.0	36.0	41.0	35.0	41.0
26	38.51179611291753	40.0	36.0	41.0	35.0	41.0
27	38.48180547344566	40.0	36.0	41.0	35.0	41.0
28	38.45217817026308	40.0	36.0	41.0	35.0	41.0
29	38.3980302862351	40.0	36.0	41.0	35.0	41.0
30	38.32647305153217	40.0	36.0	41.0	35.0	41.0
31	37.666557357867774	40.0	35.0	41.0	34.0	41.0
32	37.93553244161986	40.0	35.0	41.0	34.0	41.0
33	38.010402810621734	40.0	35.0	41.0	34.0	41.0
34	37.93599277022367	40.0	35.0	41.0	34.0	41.0
35	37.89078819341807	40.0	35.0	41.0	34.0	41.0
36	37.890755862646564	40.0	35.0	41.0	34.0	41.0
37	37.87603150556705	40.0	35.0	41.0	34.0	41.0
38	37.870478310671004	40.0	35.0	41.0	34.0	41.0
39	37.86764859838408	40.0	35.0	41.0	34.0	41.0
40	37.74594941619864	40.0	35.0	41.0	34.0	41.0
41	37.61470033993497	40.0	35.0	41.0	34.0	41.0
42	37.6473867499261	40.0	35.0	41.0	34.0	41.0
43	37.632513055473446	40.0	35.0	41.0	34.0	41.0
44	37.62785126613459	39.0	35.0	41.0	34.0	41.0
45	37.598730478372254	39.0	35.0	41.0	34.0	41.0
46	37.542564230466056	39.0	35.0	41.0	34.0	41.0
47	37.470603630899596	39.0	35.0	41.0	34.0	41.0
48	37.43034104345256	39.0	35.0	41.0	34.0	41.0
49	37.401158673268306	39.0	35.0	41.0	34.0	41.0
50	37.32952753965908	39.0	35.0	41.0	34.0	41.0
51	36.67409966499162	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	5.0
13	7.0
14	6.0
15	5.0
16	7.0
17	24.0
18	47.0
19	78.0
20	124.0
21	214.0
22	375.0
23	604.0
24	961.0
25	1428.0
26	2006.0
27	2365.0
28	2899.0
29	3666.0
30	5265.0
31	8529.0
32	15377.0
33	32920.0
34	51075.0
35	51889.0
36	50953.0
37	95773.0
38	133704.0
39	188883.0
40	342.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.630000492659374	4.860546605576904	57.43022711597202	15.079225785791703
2	19.83184919696522	4.644854172824909	53.56562222879101	21.95767440141886
3	21.381878756527737	4.365885801556804	51.48382845600552	22.76840698590994
4	19.743170509409794	4.947377820474923	50.01293230860183	25.296519361513447
5	11.646621588333826	39.38149694551187	32.46178810720268	16.51009335895162
6	22.191533648635335	6.311736377968273	53.177960882845596	18.318769090550795
7	85.17357005616317	2.2499137846093213	8.161826288304267	4.414689870923243
8	87.41978889545769	2.614481722337176	5.900365799586166	4.065363582618977
9	82.80557197753473	4.761860774460538	7.674863287023352	4.757703960981377
10	45.687844861562716	24.365393142181496	15.562647797812593	14.384114198443196
11	37.62593605281309	21.475637993891024	22.470809932013005	18.427616021282887
12	33.78149940880875	19.90682579564489	26.577741649423587	19.73393314612277
13	30.82384964035866	20.561600896640066	29.15758325943443	19.456966203566854
14	21.67531899694551	23.02997832298749	35.12445807468716	20.170244605379843
15	19.49745664597497	21.63929328012612	38.29656862745098	20.566681446447927
16	27.80954404374815	21.181274017144545	30.229579268893485	20.779602670213816
17	29.191453591486844	21.164800719282688	26.690437481525276	22.95330820770519
18	29.348796679475807	20.594855404473346	29.423927234210268	20.632420681840575
19	28.957286432160807	23.094793822051436	27.44128116070549	20.506638585082275
20	25.66832323381614	29.373737560350772	26.324176027194795	18.63376317863829
21	23.9643376194699	24.871908562419943	32.61682185436989	18.54693196374027
22	22.88372007094295	20.810855749334912	34.224277022366735	22.081147157355403
23	21.90024879298453	23.26306778993004	32.429919203862454	22.406764213222978
24	22.71498423489999	27.069785200512364	28.65383904818209	21.561391516405557
25	26.792664301901663	24.413889299438367	25.529300916346436	23.264145482313527
26	20.27493472263277	24.692241846487338	26.501225490196077	28.531597940683813
27	19.354893339245248	23.558971327224356	32.67671075968075	24.409424573849638
28	18.004082914572862	24.942728347620456	29.391288550596116	27.661900187210563
29	21.217761602128288	26.944311015863633	28.107910877919007	23.730016504089072
30	25.768086757315995	23.600231549906393	29.586812740171446	21.04486895260617
31	24.73196250862154	23.977885752290867	29.89303133313627	21.397120405951327
32	28.148401320327128	23.135746132623904	26.128189969455118	22.58766257759385
33	28.47909892600256	22.154276283377673	26.488755049758595	22.87786974086117
34	21.717656912011034	23.68521529214701	31.48278155483299	23.114346241008967
35	20.457372647551484	27.15184377771209	29.631921864223077	22.75886171051335
36	25.676174992610107	25.896794019115184	27.55505468519066	20.871976303084047
37	21.772157355404474	29.205155680362598	26.736778254015174	22.285908710217754
38	24.46207754458567	25.681717410582323	25.461406296186816	24.394798748645186
39	23.169616464676324	22.39475564094985	25.947291605084246	28.488336289289585
40	26.4433380135974	20.942180264065424	28.042787466745494	24.571694255591684
41	22.06975440930141	21.429913045620257	28.068959996058723	28.431372549019606
42	20.8468814661543	22.35688245147305	32.72428318060893	24.071952901763723
43	19.79474578776234	21.87869494531481	32.4908858015568	25.835673465366042
44	22.834146221302593	21.813109665976942	29.45471844516701	25.898025667553455
45	21.338771061188293	21.667929106315892	27.03791629717214	29.955383535323676
46	21.237929845304958	22.167824416198638	29.754009015666565	26.840236722829836
47	18.075056655828163	24.95781604098926	33.302234210267024	23.66489309291556
48	19.868644694058528	27.137217952507637	29.897034190560646	23.09710316287319
49	24.210975219233422	22.505450044339344	29.732763080106416	23.550811656320818
50	21.23962336190758	23.241359986205538	27.14522366735639	28.373792984530493
51	20.128984382697805	24.742585476401615	26.118490738003747	29.00993940289684
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	170.0
1	407.5
2	645.0
3	1036.5
4	1428.0
5	1058.0
6	688.0
7	691.5
8	695.0
9	808.0
10	921.0
11	904.5
12	888.0
13	860.5
14	833.0
15	894.0
16	955.0
17	1042.0
18	1129.0
19	1302.5
20	1476.0
21	1526.0
22	1576.0
23	1807.0
24	2038.0
25	2574.5
26	4211.0
27	5311.0
28	6133.0
29	6955.0
30	8141.0
31	9327.0
32	10971.5
33	12616.0
34	14848.5
35	17081.0
36	17322.5
37	17564.0
38	19812.5
39	22061.0
40	24465.0
41	26869.0
42	29706.5
43	32544.0
44	36918.5
45	41293.0
46	47476.5
47	53660.0
48	59578.0
49	65496.0
50	64830.5
51	64165.0
52	58234.5
53	52304.0
54	49761.0
55	47218.0
56	44887.0
57	42556.0
58	38407.5
59	34259.0
60	29056.0
61	23853.0
62	21068.5
63	18284.0
64	15666.0
65	13048.0
66	10953.0
67	8858.0
68	7336.5
69	5815.0
70	5063.0
71	4311.0
72	3891.5
73	3472.0
74	2705.0
75	1502.5
76	1067.0
77	823.5
78	580.0
79	446.0
80	312.0
81	207.0
82	102.0
83	69.0
84	36.0
85	30.0
86	24.0
87	13.5
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	649536.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.849191898764815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.44394331781706	23.503669230920234
2	11.168069684964182	7.783964071801893
3	4.462853746524572	4.665805398862689
4	2.8311601566874995	3.9465457438615896
5	2.0785404744119544	3.6217727931065933
6	1.696518666567062	3.547338274261928
7	1.4158826355038494	3.4539655969561767
8	1.223442644194314	3.4108789987727914
9	1.0653887944378864	3.3415144689743643
>10	6.453854917729567	36.136012273807246
>50	0.10899854105582954	2.5435897494972357
>100	0.04774316255497412	3.103634684979915
>500	0.0036032575513188013	0.9413087141973676
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	958	0.1474899004828062	No Hit
CCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTCTGC	951	0.14641220809932012	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	878	0.13517341610010838	No Hit
CTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTCTGCT	714	0.1099246231155779	Illumina Single End Adapter 2 (95% over 21bp)
TTTTTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	651	0.10022539166420338	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11208000788255	0.0
2	0.0	0.0	0.0	0.44093014090058136	0.0
3	0.0	0.0	0.0	0.6784843334318652	0.0
4	0.0	0.0	0.0	0.9673058922061287	0.0
5	0.0	0.0	0.0	1.4996859296482412	0.0
6	0.0	0.0	0.0	2.102885752290866	0.0
7	0.0	0.0	0.0	2.6287996354320624	0.0
8	0.0	0.0	0.0	3.5025002463296877	0.0
9	0.0	0.0	0.0	3.9731439058035276	0.0
10	0.0	0.0	0.0	4.829293526455809	0.0
11	0.0	0.0	0.0	6.63088727953493	0.0
12	0.0	0.0	0.0	8.149817716031135	0.0
13	0.0	0.0	0.0	8.604603901862253	0.0
14	0.0	0.0	0.0	8.793046112917528	0.0
15	0.0	0.0	0.0	9.026135579860085	0.0
16	0.0	0.0	0.0	9.549894078234308	0.0
17	0.0	0.0	0.0	10.3047406148389	0.0
18	0.0	0.0	0.0	11.183059907380038	0.0
19	0.0	0.0	0.0	11.800115774953197	0.0
20	0.0	0.0	0.0	12.514317913094887	0.0
21	0.0	0.0	0.0	13.47069292541137	0.0
22	0.0	0.0	0.0	14.453086510986305	0.0
23	0.0	0.0	0.0	15.363120750812888	0.0
24	0.0	0.0	0.0	16.047917282490886	0.0
25	0.0	0.0	0.0	16.65681347916051	0.0
26	0.0	0.0	0.0	17.242770223667357	0.0
27	0.0	0.0	0.0	18.001311705586758	0.0
28	0.0	0.0	0.0	18.638997684500936	0.0
29	0.0	0.0	0.0	19.391380924228987	0.0
30	0.0	0.0	0.0	20.214276037047984	0.0
31	0.0	0.0	0.0	20.839183663415113	0.0
32	0.0	0.0	0.0	21.470711400137944	0.0
33	0.0	0.0	0.0	22.149657601734162	0.0
34	0.0	0.0	0.0	22.88125677406641	0.0
35	0.0	0.0	0.0	23.70338210661149	0.0
36	0.0	0.0	0.0	24.359234899990145	0.0
37	0.0	0.0	0.0	25.0586572568726	0.0
38	0.0	0.0	0.0	26.040742930337963	0.0
39	0.0	0.0	0.0	28.184734949256086	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	190	0.0	45.000004	4
TTACGGG	1025	0.0	45.000004	3
TTGATCG	25	3.8889262E-5	45.0	2
AGATACG	20	7.031019E-4	45.0	7
CTATCGG	35	1.2108285E-7	45.0	3
TAACGAA	35	1.2108285E-7	45.0	19
ATCGTTC	20	7.031019E-4	45.0	22
CCGTATG	40	6.8066583E-9	45.0	1
TACGAAT	85	0.0	45.0	12
TAGGCGT	70	0.0	45.0	4
CCGATAA	30	2.164039E-6	44.999996	15
ATTCCGA	30	2.164039E-6	44.999996	12
TATTACG	30	2.164039E-6	44.999996	1
CGTAAGG	30	2.164039E-6	44.999996	2
CGATAAC	30	2.164039E-6	44.999996	16
ACGAACG	30	2.164039E-6	44.999996	21
TACGGGG	905	0.0	44.502758	4
TTCACGG	280	0.0	44.196426	2
TTTACGG	630	0.0	43.57143	2
CTACGGG	465	0.0	43.54839	3
>>END_MODULE