##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935267.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	860337
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10866207079319	33.0	31.0	34.0	30.0	34.0
2	32.26452076337528	34.0	31.0	34.0	30.0	34.0
3	32.326837041763866	34.0	31.0	34.0	30.0	34.0
4	35.83895729231685	37.0	35.0	37.0	35.0	37.0
5	31.55680971526274	37.0	35.0	37.0	0.0	37.0
6	33.33931703506882	37.0	35.0	37.0	17.0	37.0
7	35.11838035560484	37.0	35.0	37.0	32.0	37.0
8	35.68349728071674	37.0	35.0	37.0	35.0	37.0
9	37.68553020502431	39.0	37.0	39.0	35.0	39.0
10	37.39169767195878	39.0	37.0	39.0	35.0	39.0
11	37.30507115235076	39.0	37.0	39.0	34.0	39.0
12	37.095814779557315	39.0	37.0	39.0	34.0	39.0
13	36.97340112072362	39.0	37.0	39.0	33.0	39.0
14	37.996297962310116	40.0	37.0	41.0	33.0	41.0
15	38.12448377786844	40.0	37.0	41.0	33.0	41.0
16	38.2315615857507	40.0	37.0	41.0	34.0	41.0
17	38.25052508493765	40.0	37.0	41.0	34.0	41.0
18	38.261281335104734	40.0	37.0	41.0	34.0	41.0
19	38.309278805863286	40.0	37.0	41.0	34.0	41.0
20	38.202304445816	40.0	37.0	41.0	34.0	41.0
21	38.145387214544996	40.0	37.0	41.0	34.0	41.0
22	38.10705921051867	40.0	37.0	41.0	34.0	41.0
23	38.07681059863751	40.0	37.0	41.0	34.0	41.0
24	38.057721567246325	40.0	37.0	41.0	34.0	41.0
25	37.95351007802756	40.0	36.0	41.0	34.0	41.0
26	37.87781299653508	40.0	36.0	41.0	34.0	41.0
27	37.804958987001605	40.0	36.0	41.0	33.0	41.0
28	37.696730467247136	39.0	36.0	41.0	33.0	41.0
29	37.65446795848604	39.0	36.0	41.0	33.0	41.0
30	37.63496978509584	39.0	36.0	41.0	33.0	41.0
31	37.36215575989409	39.0	35.0	41.0	32.0	41.0
32	37.39381428440251	39.0	35.0	41.0	33.0	41.0
33	37.42211714711793	39.0	35.0	41.0	33.0	41.0
34	37.33292884067522	39.0	35.0	41.0	33.0	41.0
35	37.282271946923125	39.0	35.0	41.0	32.0	41.0
36	37.28187094127069	39.0	35.0	41.0	32.0	41.0
37	37.115475679878934	39.0	35.0	41.0	32.0	41.0
38	37.08518406159447	39.0	35.0	40.0	32.0	41.0
39	37.169678858400836	39.0	35.0	41.0	32.0	41.0
40	37.15651541198391	39.0	35.0	41.0	32.0	41.0
41	37.14763633320431	39.0	35.0	41.0	32.0	41.0
42	37.08754243976488	39.0	35.0	41.0	32.0	41.0
43	36.947979687029616	39.0	35.0	40.0	32.0	41.0
44	36.81896745112671	39.0	35.0	40.0	31.0	41.0
45	36.8870070681605	39.0	35.0	40.0	31.0	41.0
46	36.80119650788005	39.0	35.0	40.0	31.0	41.0
47	36.73781901743154	39.0	35.0	40.0	31.0	41.0
48	36.6928738389724	39.0	35.0	40.0	31.0	41.0
49	36.67534582378766	39.0	35.0	40.0	31.0	41.0
50	36.53090475011536	38.0	35.0	40.0	31.0	41.0
51	35.79558126641072	37.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	16.0
11	13.0
12	14.0
13	17.0
14	16.0
15	24.0
16	35.0
17	56.0
18	113.0
19	180.0
20	291.0
21	540.0
22	823.0
23	1331.0
24	2029.0
25	2942.0
26	4052.0
27	5300.0
28	7031.0
29	9476.0
30	12275.0
31	16542.0
32	22681.0
33	34146.0
34	55063.0
35	78926.0
36	79426.0
37	122561.0
38	194420.0
39	209865.0
40	130.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.158715712563797	4.67305253638981	55.74455126305157	16.423680487994822
2	19.0153393379571	4.425707600626266	53.433945070361965	23.12500799105467
3	20.24171923327719	4.0205175413820395	52.34227982755595	23.395483397784822
4	19.04567628731532	4.502537958962593	51.80388615158944	24.647899602132654
5	15.22508040453915	17.308217593803356	44.907402564343975	22.55929943731352
6	19.85396420239976	6.055417818831458	54.03696458480805	20.053653393960737
7	78.19924053016433	2.2270342900514564	12.641674134670483	6.932051045113717
8	77.60342749410987	2.5526043864206702	10.678606174092245	9.165361945377217
9	71.90077841589981	5.539922146786666	14.315204390837543	8.244095046475975
10	34.42197650455577	26.842388505899432	21.832142520895882	16.903492468648913
11	25.725384355200347	26.44521855970393	27.559200638819437	20.270196446276284
12	24.04929696153949	20.78441354957418	34.32155074116305	20.844738747723277
13	21.87177815204972	26.2590124567466	33.604622374720606	18.264587016483073
14	16.789583616652546	29.631063176406453	32.02663607400356	21.552717132937442
15	13.793664575625597	26.31864025376103	39.10397902217387	20.783716148439506
16	15.202414867662323	25.51848868524776	33.93693401539164	25.342162431698277
17	14.932520628544395	24.578740656277713	30.194563293221144	30.294175421956744
18	16.585245084193755	24.226785550313423	35.16075677321794	24.02721259227489
19	19.717970981138784	24.47354931846474	32.372779503845585	23.435700196550886
20	21.060933099471484	27.803174802432068	31.14326130341947	19.99263079467697
21	20.658765111810837	27.039055625876834	31.456859346976824	20.8453199153355
22	17.153394541906252	26.58411761902603	28.691315147436413	27.5711726916313
23	17.512672359784595	26.539716413451934	28.32459838412157	27.6230128426419
24	20.6768975413123	24.35998916703571	29.294102194837606	25.669011096814387
25	18.04281345565749	26.743125077731168	27.874542185213468	27.339519281397873
26	19.790035765054856	27.91952455839979	27.728320413977315	24.562119262568043
27	22.685761509733975	28.02831913540857	27.730180150336437	21.555739204521018
28	17.340762980088034	28.24614075647101	30.305217606589046	24.10787865685191
29	19.784456555977485	26.783806810587013	28.367256086858987	25.06448054657652
30	22.41586727061605	26.69605050114083	26.535996940733693	24.35208528750943
31	21.100917431192663	32.298971217092834	24.61384318005619	21.986268171658317
32	21.88572617474315	32.52318568188977	22.81106124692998	22.780026896437093
33	23.776148183793094	27.846762373349048	23.851816206904967	24.525273235952888
34	19.94985685841711	26.430805602920714	27.8209585313662	25.798379007295978
35	21.636521502620486	26.3573460167353	25.64483452414577	26.36129795649844
36	27.58151747512893	25.78849915788813	25.253941188162315	21.376042178820626
37	21.48902116263743	30.199561334686294	28.98143401945982	19.329983483216463
38	18.190778729730326	34.397102530752484	24.513882350753253	22.898236388763937
39	20.474651212257523	26.363738860469795	28.844045995929502	24.317563931343184
40	22.057054386827488	23.577970028023902	30.27360208848393	24.091373496664676
41	21.081041498854518	25.538597084630787	27.147269035273386	26.233092381241303
42	26.395238145052463	24.713920242881567	24.848053727783416	24.042787884282554
43	26.896088393269153	23.75534238327539	25.880323640619896	23.468245582835564
44	20.76883825756651	25.407485671312518	26.44347505686725	27.380201014253718
45	21.766121880146965	23.919580350490563	25.399116857696463	28.91518091166601
46	23.344224414386456	23.50055850207535	29.504833571030886	23.650383512507307
47	18.94873752959596	25.501053656880966	32.335933477230434	23.21427533629264
48	19.663108758544617	24.423801370858165	30.443303031254032	25.46978683934319
49	23.05189710543659	20.66725015894934	32.34883539822186	23.93201733739221
50	21.65314289633016	21.190998411087747	29.590265210028164	27.56559348255393
51	19.349626948509712	20.50568556275041	26.64874345750561	33.49594403123427
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	338.0
1	523.5
2	709.0
3	1100.0
4	1491.0
5	1158.5
6	826.0
7	968.5
8	1111.0
9	1627.0
10	2143.0
11	2197.5
12	2252.0
13	2437.0
14	2622.0
15	2483.5
16	2345.0
17	2287.5
18	2230.0
19	2403.0
20	2576.0
21	2647.5
22	2719.0
23	3141.0
24	3563.0
25	3769.0
26	5153.0
27	6331.0
28	6993.5
29	7656.0
30	9066.0
31	10476.0
32	12375.5
33	14275.0
34	16139.0
35	18003.0
36	19735.0
37	21467.0
38	23988.0
39	26509.0
40	32036.5
41	37564.0
42	46020.0
43	54476.0
44	65582.0
45	76688.0
46	92927.0
47	109166.0
48	126234.0
49	143302.0
50	131545.0
51	119788.0
52	92322.0
53	64856.0
54	51735.5
55	38615.0
56	31800.0
57	24985.0
58	21242.5
59	17500.0
60	15105.0
61	12710.0
62	11104.0
63	9498.0
64	8205.5
65	6913.0
66	5310.5
67	3708.0
68	3270.5
69	2833.0
70	2169.5
71	1506.0
72	1293.5
73	1081.0
74	907.0
75	556.5
76	380.0
77	314.0
78	248.0
79	180.5
80	113.0
81	80.0
82	47.0
83	26.5
84	6.0
85	4.0
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	860337.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.776003663733245
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.36035541049186	16.965371086067705
2	13.38325547708388	7.1670019537415195
3	6.482385814130712	5.20717158926689
4	3.8006836406876214	4.07068476350971
5	2.518931277587469	3.3723456558687164
6	1.684697115105118	2.7065673675801283
7	1.268973119140782	2.378462022110481
8	0.9368703059251936	2.006851419511669
9	0.7331794231854657	1.7668453429249067
>10	5.058189250426989	28.89811557365136
>50	0.619387043589143	10.863807063599683
>100	0.13510708818018782	6.900155374764343
>500	0.011405143807418453	2.1483776526429366
>1k	0.004825253149292423	1.9704326445103004
>5k	0.001754637508833608	3.5778104902496577
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	8549	0.9936803833846505	No Hit
CCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	8037	0.9341688198926699	No Hit
CTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGCT	8023	0.932541550578436	No Hit
GCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	5852	0.6801985733497455	No Hit
TCCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTG	2521	0.2930247100845366	No Hit
GCCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTG	2007	0.2332806795476656	No Hit
TTCCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCT	1802	0.2094528074463844	No Hit
TGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTG	1680	0.19527231770806092	No Hit
TGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGCTT	1571	0.182602863761526	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCC	1447	0.168189906978312	No Hit
TTCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTG	1389	0.16144836267648607	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTC	1129	0.13122764684071475	No Hit
TTTCCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTC	1109	0.12890297639180925	No Hit
CGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTG	1071	0.12448610253888884	No Hit
TCTGACTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	1050	0.12204519856753807	No Hit
TGCCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCT	958	0.11135171450257282	No Hit
TTGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCT	878	0.10205303270695087	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9656681044753394	0.0
2	0.0	0.0	0.0	3.7578297806557197	0.0
3	0.0	0.0	0.0	5.211911146446102	0.0
4	0.0	0.0	0.0	6.8667278054994725	0.0
5	0.0	0.0	0.0	9.569738369964329	0.0
6	0.0	0.0	0.0	11.296503579411324	0.0
7	0.0	0.0	0.0	12.891111273838042	0.0
8	0.0	0.0	0.0	15.403731328537539	0.0
9	0.0	0.0	0.0	16.575249001263458	0.0
10	0.0	0.0	0.0	18.7301022738764	0.0
11	0.0	0.0	0.0	22.647404447327038	0.0
12	0.0	0.0	0.0	25.942741042173008	0.0
13	0.0	0.0	0.0	27.036614721905487	0.0
14	0.0	0.0	0.0	27.392754234677806	0.0
15	0.0	0.0	0.0	27.932426479391214	0.0
16	0.0	0.0	0.0	29.330250820318085	0.0
17	0.0	0.0	0.0	31.42280292490036	0.0
18	1.1623352244527435E-4	0.0	0.0	33.988076765267564	0.0
19	1.1623352244527435E-4	0.0	0.0	35.38822577664334	0.0
20	1.1623352244527435E-4	0.0	0.0	36.79197802721492	0.0
21	1.1623352244527435E-4	0.0	0.0	38.53501592980425	0.0
22	1.1623352244527435E-4	0.0	0.0	40.23283899216237	0.0
23	1.1623352244527435E-4	0.0	0.0	41.83988367349074	0.0
24	2.324670448905487E-4	0.0	0.0	42.9858299712787	0.0
25	3.4870056733582303E-4	0.0	0.0	44.00810380118489	0.0
26	3.4870056733582303E-4	0.0	0.0	44.88729416496094	0.0
27	3.4870056733582303E-4	0.0	0.0	45.72092098793845	0.0
28	3.4870056733582303E-4	0.0	0.0	46.608015231240785	0.0
29	3.4870056733582303E-4	0.0	0.0	47.535907440921406	0.0
30	3.4870056733582303E-4	0.0	0.0	48.60688311673216	0.0
31	3.4870056733582303E-4	0.0	0.0	49.566390844517905	0.0
32	4.649340897810974E-4	0.0	0.0	50.35596516248865	0.0
33	4.649340897810974E-4	0.0	0.0	51.16785631676889	0.0
34	4.649340897810974E-4	0.0	0.0	51.95847673644165	0.0
35	4.649340897810974E-4	0.0	0.0	52.865795612649464	0.0
36	4.649340897810974E-4	0.0	0.0	53.59957783984648	0.0
37	4.649340897810974E-4	0.0	0.0	54.345215886332916	0.0
38	4.649340897810974E-4	0.0	0.0	55.076092275468795	0.0
39	4.649340897810974E-4	0.0	0.0	55.8421874219056	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCTACG	85	0.0	45.000004	1
CGGAAAG	20	7.032295E-4	45.000004	21
TCGCACG	20	7.032295E-4	45.000004	1
TGCCCCG	20	7.032295E-4	45.000004	42
ACTGCGA	20	7.032295E-4	45.000004	37
CGTATAA	20	7.032295E-4	45.000004	26
CACGGGT	160	0.0	45.000004	4
CAACGGG	20	7.032295E-4	45.000004	3
CATGCGG	20	7.032295E-4	45.000004	2
CTATCGT	20	7.032295E-4	45.000004	17
CTATCGA	20	7.032295E-4	45.000004	41
CGGTTGC	20	7.032295E-4	45.000004	6
TAGCGGA	20	7.032295E-4	45.000004	9
CACTGCG	20	7.032295E-4	45.000004	36
ACGGGTA	80	0.0	45.000004	5
TCGGCGT	75	0.0	45.000004	4
AACGGCG	20	7.032295E-4	45.000004	9
AATGCGC	20	7.032295E-4	45.000004	11
GTGCTAT	20	7.032295E-4	45.000004	26
GCTTACG	40	6.8121153E-9	45.000004	1
>>END_MODULE