FastQCFastQC Report
Sat 14 Jan 2017
SRR2935266.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935266.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2386872
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC133860.5608176726695022TruSeq Adapter, Index 15 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGCT88170.3693955938986255TruSeq Adapter, Index 14 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC63120.2644465224779544TruSeq Adapter, Index 15 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC57270.23993745789468393TruSeq Adapter, Index 15 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG33280.13942934518482766No Hit
TCCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTG25400.10641542571197785No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT207.0348143E-445.00000435
CGTAGTC800.045.00000421
CCCGATA207.0348143E-445.00000411
CGGTCAA453.8562575E-1045.012
GCGATAT600.044.9999969
TACGGGA13500.042.9999964
TGTTACG2500.042.3000031
TTACGGG16900.042.0710073
TCTACGG4100.041.7073172
TATGGGA35300.041.6855554
TCGTTCA2700.041.66666816
ATGGGCG5900.041.56785
TAGGCGT1300.041.538464
TTATGGG50850.041.371683
TTTACGG10150.041.009852
CTACGGG6650.040.6015053
TACGGGC4300.040.29074
CGTATGG3950.039.8734172
CGTTTGG12900.039.593022
TACGGGG10150.039.4581264
Produced by FastQC (version 0.11.5)