FastQCFastQC Report
Sat 14 Jan 2017
SRR2935264.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935264.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2582175
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC223140.8641552179848383Illumina PCR Primer Index 10 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC189900.735426529960208Illumina PCR Primer Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGCT186930.7239245984489819Illumina PCR Primer Index 10 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC155430.6019344157541607Illumina PCR Primer Index 10 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG82380.3190333730285515Illumina PCR Primer Index 10 (95% over 22bp)
TTCCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCT61970.23999148005073245Illumina PCR Primer Index 10 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCC58110.22504284179035117No Hit
TGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG44790.17345842167939818Illumina PCR Primer Index 10 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG42900.1661390107177089Illumina PCR Primer Index 10 (95% over 22bp)
TTCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG38990.15099673724670093Illumina PCR Primer Index 10 (95% over 22bp)
TTTCCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTC38950.15084182907819957No Hit
TGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGCTT37970.14704657894991624Illumina PCR Primer Index 10 (96% over 25bp)
CGTTCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTC33880.13120721872065216No Hit
CGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG33280.12888359619313178Illumina PCR Primer Index 10 (95% over 22bp)
TGCCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCT27370.10599591429705578Illumina PCR Primer Index 10 (95% over 21bp)
GTTCCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTC27220.10541500866517567No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27020.10464046782266886No Hit
TTGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCT26620.10309138613765526Illumina PCR Primer Index 10 (95% over 21bp)
GTTTCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTC26190.10142612332626563No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTA207.0349214E-445.0000049
CGAAACG253.892162E-545.012
ACGTTAC253.892162E-545.014
GCGATAC453.8562575E-1045.09
ACTACGG302.1665583E-644.9999962
TCCGTCG302.1665583E-644.9999961
GCTACGA1400.043.39285710
TACGGGA9100.043.0219764
TTTACGG10200.042.7941172
GCACGGG4550.042.527473
CAACCCG900.042.523
TTATGGG54750.042.1643873
TGCACGG3150.042.1428572
TTACGGG15800.042.009493
GTACGGG3700.041.9594573
TATGGGA41600.041.3221134
ACGGGCC1100.040.9090925
CGTTTTT24850.040.8350071
TCACGGG7000.040.8214263
TTCTACG3000.040.5000041
Produced by FastQC (version 0.11.5)