##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935264.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2582175
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13717466864175	33.0	31.0	34.0	30.0	34.0
2	32.28925808669048	34.0	31.0	34.0	30.0	34.0
3	32.34888611345087	34.0	31.0	34.0	30.0	34.0
4	35.8703407011531	37.0	35.0	37.0	35.0	37.0
5	31.651616563555915	37.0	35.0	37.0	0.0	37.0
6	33.41088694608228	37.0	35.0	37.0	17.0	37.0
7	35.19615014474232	37.0	35.0	37.0	32.0	37.0
8	35.73946459864262	37.0	35.0	37.0	35.0	37.0
9	37.77820674431439	39.0	38.0	39.0	35.0	39.0
10	37.39683522611752	39.0	37.0	39.0	35.0	39.0
11	37.261388170824986	39.0	37.0	39.0	34.0	39.0
12	37.1743603744905	39.0	37.0	39.0	34.0	39.0
13	37.166620387851324	39.0	37.0	39.0	34.0	39.0
14	38.26016052358961	40.0	38.0	41.0	34.0	41.0
15	38.32611771084454	40.0	38.0	41.0	34.0	41.0
16	38.381834306350264	40.0	38.0	41.0	34.0	41.0
17	38.399690183663	40.0	38.0	41.0	34.0	41.0
18	38.393970974081924	40.0	38.0	41.0	34.0	41.0
19	38.43416073658834	40.0	38.0	41.0	34.0	41.0
20	38.34056134847561	40.0	37.0	41.0	34.0	41.0
21	38.306691839244046	40.0	37.0	41.0	34.0	41.0
22	38.271800323370805	40.0	37.0	41.0	34.0	41.0
23	38.22930320369456	40.0	37.0	41.0	34.0	41.0
24	38.18656868725009	40.0	37.0	41.0	34.0	41.0
25	38.09331396981227	40.0	37.0	41.0	34.0	41.0
26	38.05196704328715	40.0	37.0	41.0	34.0	41.0
27	38.00810944262105	40.0	37.0	41.0	34.0	41.0
28	37.97301073707243	40.0	37.0	41.0	34.0	41.0
29	37.955637785975	40.0	37.0	41.0	34.0	41.0
30	37.893241550243495	40.0	37.0	41.0	33.0	41.0
31	37.6467419907636	40.0	36.0	41.0	33.0	41.0
32	37.72948502715734	40.0	36.0	41.0	33.0	41.0
33	37.728161724127915	40.0	36.0	41.0	33.0	41.0
34	37.68890257244377	40.0	36.0	41.0	33.0	41.0
35	37.674427953179006	40.0	36.0	41.0	33.0	41.0
36	37.68194487205553	40.0	36.0	41.0	33.0	41.0
37	37.57848906445148	40.0	36.0	41.0	33.0	41.0
38	37.599546506336715	40.0	36.0	41.0	33.0	41.0
39	37.595928626061365	40.0	36.0	41.0	33.0	41.0
40	37.50342947321541	39.0	36.0	41.0	33.0	41.0
41	37.4485660344477	39.0	36.0	41.0	33.0	41.0
42	37.44967285331165	39.0	36.0	41.0	33.0	41.0
43	37.40386883150832	39.0	36.0	41.0	33.0	41.0
44	37.355825999399734	39.0	36.0	41.0	32.0	41.0
45	37.34021783961195	39.0	35.0	41.0	32.0	41.0
46	37.168340643062535	39.0	35.0	41.0	32.0	41.0
47	37.10815184873217	39.0	35.0	41.0	32.0	41.0
48	37.11822591420024	39.0	35.0	41.0	32.0	41.0
49	37.140906406420946	39.0	35.0	41.0	32.0	41.0
50	37.07850203801059	39.0	35.0	41.0	32.0	41.0
51	36.37210258793459	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	5.0
10	7.0
11	14.0
12	16.0
13	15.0
14	29.0
15	39.0
16	62.0
17	98.0
18	235.0
19	377.0
20	698.0
21	1205.0
22	1996.0
23	3275.0
24	5072.0
25	7498.0
26	10652.0
27	14199.0
28	18779.0
29	24878.0
30	33890.0
31	45580.0
32	63023.0
33	91246.0
34	143287.0
35	190424.0
36	236044.0
37	368164.0
38	596480.0
39	724371.0
40	512.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.3005412104137	4.582183624270237	58.4258619187313	14.691413246584759
2	19.04088607472383	4.215051264922014	54.27742116626487	22.466641494089284
3	18.982020970693313	4.10421447035929	54.20565995720662	22.708104601740782
4	18.33229738495648	4.319149554154928	52.39943071248076	24.949122348407833
5	14.907239052349281	16.416664246226535	46.472179461113214	22.20391724031098
6	19.853418145555587	5.656859043248424	54.95204624008829	19.53767657110769
7	79.45871213221413	1.7168859585427014	12.470843378159884	6.3535585310832925
8	80.12969686407776	2.1035367471220967	9.859517654690329	7.90724873410981
9	75.38819018850388	4.43904847657498	12.733335269685439	7.439426065235702
10	35.9463630466564	29.71498833347856	19.750133124207306	14.588515495657731
11	23.040924801765954	25.93793991499414	29.79286841519262	21.228266868047285
12	20.641474725764134	22.197488551318173	35.33896811796257	21.822068604955124
13	21.353355214112135	24.40764084541133	35.260739492869384	18.978264447607152
14	18.49138807400738	27.227550417767965	32.39319565869857	21.88786584952608
15	16.07346519891177	26.6765033353665	35.24633303319876	22.00369843252297
16	17.283375448991645	26.966104156379796	31.803344080087527	23.947176314541036
17	17.140046666085762	27.46796789528208	29.26590955299312	26.126075885639043
18	17.240891883780147	25.578746599281615	34.085373764365315	23.09498775257293
19	19.04917366173865	26.595989814787924	31.301054343721862	23.05378217975157
20	20.284605032579126	29.50125378798881	30.629217616931463	19.584923562500606
21	21.19771123181039	28.766369436618355	30.543359764539584	19.49255956703167
22	18.66937755961544	26.983298963083445	29.1301712703438	25.217152206957312
23	17.65554232381616	27.656800952685234	28.07191611722676	26.615740606271842
24	20.693252781085715	26.640138642810808	28.46472450550408	24.201884070599398
25	17.705926205621232	28.408609021464464	25.97252316361207	27.912941609302234
26	16.397300725163863	28.65638463698239	27.022529456756416	27.923785181097333
27	18.46377569297201	27.05788724621685	28.31678720458528	26.161549856225857
28	15.276888669435651	27.17224820161298	30.135060559412125	27.415802569539245
29	16.144413140085394	23.922236099412316	31.3854792955551	28.54787146494719
30	19.22553661157745	26.422647574234897	29.54853950642385	24.803276307763802
31	18.79438845159604	32.21783961195504	25.19376107351361	23.794010862935316
32	19.160165364469876	28.892348504652087	26.108377627387764	25.839108503490277
33	18.385275978583945	27.019198931133637	26.96858268707582	27.6269424032066
34	17.197440142515514	27.172790380202738	27.871310039017494	27.758459438264254
35	16.9767347294432	24.322208990482828	26.978729172112658	31.722327107961313
36	18.261233262656482	25.58660818883306	26.771539496742086	29.380619051768374
37	16.483313485724242	29.8612990986281	29.22423925566625	24.43114815998141
38	17.68207034767202	29.169905215564395	29.671265502919052	23.47675893384453
39	18.121777183963133	25.66243573731447	32.68999970954719	23.525787369175212
40	20.399779255859883	25.90215612807033	30.810769990415054	22.88729462565473
41	20.469913929148873	26.309990608692285	24.719587169730943	28.500508292427895
42	20.6776457831092	23.569355291566218	25.026382797447887	30.726616127876692
43	19.76271941289804	23.660325113518642	27.79191960266055	28.78503587092277
44	17.488473864087446	23.72306292176169	28.784532419375143	30.003930794775723
45	19.291140220937773	22.794891903143668	27.728097437237984	30.185870438680567
46	20.72892038688315	23.298188542604585	30.316148208390214	25.65674286212205
47	16.151112918373077	25.880740073775016	31.297568909930582	26.67057809792133
48	16.548684732831816	24.007087048708936	30.848528856487263	28.59569936197198
49	19.582019034341204	21.25781560118892	33.25463998373464	25.905525380735234
50	19.514672708085236	20.600966239701027	30.859953333914238	29.024407718299493
51	17.567593211149514	20.710292679620864	27.57748797041254	34.14462613881708
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	761.0
1	1086.5
2	1412.0
3	3095.5
4	4779.0
5	3463.5
6	2148.0
7	2599.0
8	3050.0
9	4673.0
10	6296.0
11	7365.0
12	8434.0
13	8984.5
14	9535.0
15	9522.0
16	9509.0
17	9142.5
18	8776.0
19	8096.0
20	7416.0
21	8227.5
22	9039.0
23	9574.5
24	10110.0
25	11930.5
26	16189.0
27	18627.0
28	22246.5
29	25866.0
30	30061.5
31	34257.0
32	40666.5
33	47076.0
34	53559.0
35	60042.0
36	69383.5
37	78725.0
38	85335.0
39	91945.0
40	107164.5
41	122384.0
42	144031.0
43	165678.0
44	196833.0
45	227988.0
46	280969.5
47	333951.0
48	374068.5
49	414186.0
50	374360.5
51	334535.0
52	261939.5
53	189344.0
54	151189.5
55	113035.0
56	93498.0
57	73961.0
58	62618.5
59	51276.0
60	43523.0
61	35770.0
62	30066.5
63	24363.0
64	20459.0
65	16555.0
66	13348.5
67	10142.0
68	8178.0
69	6214.0
70	5235.0
71	4256.0
72	3541.0
73	2826.0
74	2514.0
75	1512.5
76	823.0
77	735.5
78	648.0
79	456.0
80	264.0
81	174.5
82	85.0
83	64.5
84	44.0
85	55.5
86	67.0
87	44.5
88	22.0
89	12.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2582175.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.62163107456627
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.48378242350403	15.75314790323922
2	14.43981249048808	7.39943096834399
3	7.156400751239678	5.500759796100358
4	3.9783027334188716	4.077224197543875
5	2.527311146675156	3.237691690537494
6	1.7830687943981234	2.741107849838422
7	1.2562718790294083	2.253141422969055
8	0.925013754286208	1.8960288920976571
9	0.6969207066520503	1.607062071065837
>10	4.962909142820413	27.535793900538692
>50	0.6117505418208746	10.190680676964183
>100	0.15566892937470328	7.465542014999025
>500	0.013318990297647614	2.3078655277718574
>1k	0.008344427656357543	4.096284517421616
>5k	4.814092878667813E-4	0.8324136940652217
>10k+	6.418790504890417E-4	3.1058248765033514
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	22314	0.8641552179848383	Illumina PCR Primer Index 10 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	18990	0.735426529960208	Illumina PCR Primer Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGCT	18693	0.7239245984489819	Illumina PCR Primer Index 10 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	15543	0.6019344157541607	Illumina PCR Primer Index 10 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG	8238	0.3190333730285515	Illumina PCR Primer Index 10 (95% over 22bp)
TTCCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCT	6197	0.23999148005073245	Illumina PCR Primer Index 10 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCC	5811	0.22504284179035117	No Hit
TGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG	4479	0.17345842167939818	Illumina PCR Primer Index 10 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG	4290	0.1661390107177089	Illumina PCR Primer Index 10 (95% over 22bp)
TTCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG	3899	0.15099673724670093	Illumina PCR Primer Index 10 (95% over 22bp)
TTTCCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTC	3895	0.15084182907819957	No Hit
TGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGCTT	3797	0.14704657894991624	Illumina PCR Primer Index 10 (96% over 25bp)
CGTTCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTC	3388	0.13120721872065216	No Hit
CGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG	3328	0.12888359619313178	Illumina PCR Primer Index 10 (95% over 22bp)
TGCCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCT	2737	0.10599591429705578	Illumina PCR Primer Index 10 (95% over 21bp)
GTTCCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTC	2722	0.10541500866517567	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2702	0.10464046782266886	No Hit
TTGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCT	2662	0.10309138613765526	Illumina PCR Primer Index 10 (95% over 21bp)
GTTTCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTC	2619	0.10142612332626563	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3236225275204044E-4	0.0	0.0	0.7571136735503984	0.0
2	2.3236225275204044E-4	0.0	0.0	3.1285640980955978	0.0
3	2.3236225275204044E-4	0.0	0.0	4.470572288865007	0.0
4	2.3236225275204044E-4	0.0	0.0	6.009468761799646	0.0
5	2.3236225275204044E-4	0.0	0.0	8.63551076127683	0.0
6	2.3236225275204044E-4	0.0	0.0	10.280015878087271	0.0
7	2.3236225275204044E-4	0.0	0.0	11.80078809530725	0.0
8	2.710892948773805E-4	0.0	0.0	14.164067113964004	0.0
9	2.710892948773805E-4	0.0	0.0	15.205630911925024	0.0
10	2.710892948773805E-4	0.0	0.0	17.0876877051323	0.0
11	2.710892948773805E-4	0.0	0.0	21.056551163263528	0.0
12	2.710892948773805E-4	0.0	0.0	24.20343315228441	0.0
13	2.710892948773805E-4	0.0	0.0	25.202513385033935	0.0
14	2.710892948773805E-4	0.0	0.0	25.51670587779682	0.0
15	2.710892948773805E-4	0.0	0.0	25.96562975011376	0.0
16	2.710892948773805E-4	0.0	0.0	27.0775218565744	0.0
17	2.710892948773805E-4	0.0	0.0	28.848586947050453	0.0
18	2.710892948773805E-4	0.0	0.0	30.790670655552006	0.0
19	2.710892948773805E-4	0.0	0.0	32.11610367229177	0.0
20	2.710892948773805E-4	0.0	0.0	33.356995556071915	0.0
21	3.4854337912806064E-4	0.0	0.0	35.04158316148209	0.0
22	3.4854337912806064E-4	0.0	0.0	36.7911934706207	0.0
23	3.872704212534007E-4	0.0	0.0	38.50695634494176	0.0
24	3.872704212534007E-4	0.0	0.0	39.83564243322006	0.0
25	3.872704212534007E-4	0.0	0.0	40.93839495773911	0.0
26	3.872704212534007E-4	0.0	0.0	41.89634707175153	0.0
27	3.872704212534007E-4	0.0	0.0	42.83536166216465	0.0
28	3.872704212534007E-4	0.0	0.0	43.83653315518894	0.0
29	3.872704212534007E-4	0.0	0.0	44.88239565482587	0.0
30	3.872704212534007E-4	0.0	0.0	46.01930543049948	0.0
31	4.2599746337874077E-4	0.0	0.0	47.01075643595031	0.0
32	4.2599746337874077E-4	0.0	0.0	47.93335076050229	0.0
33	4.2599746337874077E-4	0.0	0.0	48.840841538625384	0.0
34	4.2599746337874077E-4	0.0	0.0	49.738030923543135	0.0
35	4.2599746337874077E-4	0.0	0.0	50.70427062457037	0.0
36	4.647245055040809E-4	0.0	0.0	51.5757452535169	0.0
37	5.034515476294209E-4	0.0	0.0	52.40829920512746	0.0
38	5.42178589754761E-4	0.0	0.0	53.27075043325878	0.0
39	5.42178589754761E-4	0.0	0.0	54.163796024669125	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGTA	20	7.0349214E-4	45.000004	9
CGAAACG	25	3.892162E-5	45.0	12
ACGTTAC	25	3.892162E-5	45.0	14
GCGATAC	45	3.8562575E-10	45.0	9
ACTACGG	30	2.1665583E-6	44.999996	2
TCCGTCG	30	2.1665583E-6	44.999996	1
GCTACGA	140	0.0	43.392857	10
TACGGGA	910	0.0	43.021976	4
TTTACGG	1020	0.0	42.794117	2
GCACGGG	455	0.0	42.52747	3
CAACCCG	90	0.0	42.5	23
TTATGGG	5475	0.0	42.164387	3
TGCACGG	315	0.0	42.142857	2
TTACGGG	1580	0.0	42.00949	3
GTACGGG	370	0.0	41.959457	3
TATGGGA	4160	0.0	41.322113	4
ACGGGCC	110	0.0	40.909092	5
CGTTTTT	2485	0.0	40.835007	1
TCACGGG	700	0.0	40.821426	3
TTCTACG	300	0.0	40.500004	1
>>END_MODULE