##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935263.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2053005
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28910743032774	33.0	31.0	34.0	30.0	34.0
2	32.43308710889647	34.0	31.0	34.0	30.0	34.0
3	32.488405532378145	34.0	31.0	34.0	30.0	34.0
4	35.95104590587943	37.0	35.0	37.0	35.0	37.0
5	31.798336097574044	37.0	35.0	37.0	0.0	37.0
6	33.49350196419395	37.0	35.0	37.0	17.0	37.0
7	35.24902179975207	37.0	35.0	37.0	32.0	37.0
8	35.79027669197104	37.0	35.0	37.0	35.0	37.0
9	37.824589321506764	39.0	38.0	39.0	35.0	39.0
10	37.52691834652132	39.0	37.0	39.0	35.0	39.0
11	37.416422755911455	39.0	37.0	39.0	35.0	39.0
12	37.11853405130528	39.0	37.0	39.0	34.0	39.0
13	37.00069166904124	39.0	37.0	39.0	33.0	39.0
14	37.93430556671805	40.0	37.0	41.0	33.0	41.0
15	38.0838517197961	40.0	37.0	41.0	33.0	41.0
16	38.23775343947043	40.0	37.0	41.0	33.0	41.0
17	38.27034761240231	40.0	37.0	41.0	34.0	41.0
18	38.276731912489254	40.0	37.0	41.0	34.0	41.0
19	38.293997822703794	40.0	37.0	41.0	34.0	41.0
20	38.20018265907779	40.0	37.0	41.0	34.0	41.0
21	38.15787638120706	40.0	37.0	41.0	34.0	41.0
22	38.17004829506017	40.0	37.0	41.0	34.0	41.0
23	38.12973178341017	40.0	36.0	41.0	34.0	41.0
24	38.09477521973887	40.0	36.0	41.0	34.0	41.0
25	38.00207549421458	40.0	36.0	41.0	34.0	41.0
26	37.940822355522755	40.0	36.0	41.0	34.0	41.0
27	37.89470020774426	40.0	36.0	41.0	34.0	41.0
28	37.88568756530062	40.0	36.0	41.0	34.0	41.0
29	37.82400627373046	40.0	36.0	41.0	34.0	41.0
30	37.79293620814367	40.0	36.0	41.0	33.0	41.0
31	37.57366884152742	40.0	36.0	41.0	33.0	41.0
32	37.61145394190467	40.0	35.0	41.0	33.0	41.0
33	37.61567750687407	40.0	35.0	41.0	33.0	41.0
34	37.54911897438146	40.0	35.0	41.0	33.0	41.0
35	37.45988100369946	40.0	35.0	41.0	33.0	41.0
36	37.42876417738876	39.0	35.0	41.0	33.0	41.0
37	37.42337500395762	39.0	35.0	41.0	33.0	41.0
38	37.435652616530405	39.0	35.0	41.0	33.0	41.0
39	37.42408031154332	39.0	35.0	41.0	33.0	41.0
40	37.33480580904576	39.0	35.0	41.0	33.0	41.0
41	37.2397427186003	39.0	35.0	41.0	33.0	41.0
42	37.21822401796391	39.0	35.0	41.0	33.0	41.0
43	37.18099420118315	39.0	35.0	41.0	33.0	41.0
44	37.15743020596638	39.0	35.0	41.0	33.0	41.0
45	37.154874440149925	39.0	35.0	41.0	33.0	41.0
46	37.06845380308377	39.0	35.0	41.0	33.0	41.0
47	37.02578464251183	39.0	35.0	41.0	33.0	41.0
48	37.00769116490218	39.0	35.0	41.0	33.0	41.0
49	36.98196740874961	39.0	35.0	41.0	33.0	41.0
50	36.92120574474977	39.0	35.0	41.0	32.0	41.0
51	36.26475045116792	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	13.0
11	14.0
12	15.0
13	22.0
14	32.0
15	40.0
16	68.0
17	166.0
18	271.0
19	493.0
20	801.0
21	1309.0
22	2190.0
23	3250.0
24	4939.0
25	7384.0
26	9589.0
27	12376.0
28	15575.0
29	20097.0
30	26933.0
31	36425.0
32	51430.0
33	80944.0
34	142605.0
35	164574.0
36	167951.0
37	262468.0
38	423891.0
39	616548.0
40	585.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.872952087306167	4.487373386815912	53.71341034240052	15.92626418347739
2	20.855234156760456	4.652935574925536	51.937087342700096	22.554742925613915
3	21.556255342778023	4.8491357790166125	51.043275588710216	22.551333289495155
4	19.498491236017447	5.115866741678661	49.6096210189454	25.77602100335849
5	16.508045523513093	16.889876059727083	43.70695638831859	22.895122028441236
6	22.091568213423738	6.510310496077701	52.60298927669441	18.795132013804157
7	82.01996585492972	2.7291701676323243	9.845032038402245	5.405831939035706
8	83.22371353211511	2.8052050530807278	8.094817109554043	5.876264305250109
9	78.1456450422673	5.076363671788427	10.336117057678866	6.441874228265395
10	40.03380410666316	24.212410588381424	19.042135796064795	16.711649508890627
11	31.354039566391705	24.53199091088429	24.10870894128363	20.005260581440375
12	26.966763354205174	21.480707548203732	30.7593016091047	20.79322748848639
13	25.1187405778359	23.162875881938913	32.19568388776452	19.522699652460663
14	20.359229519655333	25.244020350656722	34.014919593473955	20.38183053621399
15	18.319536484324196	23.7611208935195	36.56771415559143	21.351628466564865
16	22.540519872089938	23.56306974410681	31.192081850750487	22.70432853305277
17	22.718941259276036	23.4463140615829	29.036753441905887	24.797991237235177
18	23.878022703305643	22.071889742109736	31.807326333837473	22.24276122074715
19	24.43057859089481	24.42502575492997	29.202656593627392	21.94173906054783
20	23.6242970669823	27.593454472833724	29.309865295018767	19.47238316516521
21	23.613142685965208	26.659165467205387	30.349317220367215	19.378374626462186
22	22.16166059020801	23.459027133397143	30.529979225574223	23.849333050820626
23	20.556111650970163	25.06442994537276	30.05228920533559	24.327169198321485
24	21.560395615207952	25.607244015479747	29.31590522185771	23.516455147454586
25	22.02245001838768	26.66671537575408	26.420003848017902	24.890830757840337
26	20.26361358106775	26.938365956244624	27.403537741018653	25.39448272166897
27	21.567068760183243	25.046943382992247	29.00533608052586	24.380651776298645
28	18.17409114931527	24.568279181005405	29.974890465439685	27.282739204239636
29	20.11631730073721	25.08308552585113	29.664516160457477	25.136081012954183
30	23.51587063840565	24.008270803042368	29.016587879717783	23.459270678834194
31	22.969744350354723	25.034132893003186	28.316638293623246	23.679484463018845
32	23.49443863994486	25.55176436491874	27.487122535015747	23.46667446012065
33	23.77480814708196	23.563313289543864	27.39949488676355	25.26238367661063
34	19.59966001056987	23.783186110116635	31.283070426034033	25.33408345327946
35	20.622794391635676	25.776556803320013	29.025647769976203	24.57500103506811
36	23.831846488440117	25.71279660789915	27.35317254463579	23.102184359024942
37	21.255087055316473	25.434131918821436	29.78857820609302	23.52220281976907
38	21.4729628033054	25.11864315966108	28.605093509270556	24.80330052776296
39	20.409886970562663	22.52537134590515	30.82140569555359	26.2433359879786
40	23.24178460354456	22.38918073750429	30.736262210759353	23.632772448191798
41	21.876566301592057	23.025418837265374	28.808064276511747	26.289950584630823
42	22.108129303143443	23.03379680030005	30.18083248701294	24.67724140954357
43	21.949776059970628	22.112610539185244	30.73752864703203	25.200084753812096
44	21.203845095360215	21.909055262895123	29.23426879135706	27.652830850387605
45	21.14680675400206	22.074179069218047	27.919318267612596	28.859695909167293
46	22.094588176843214	22.485819566927503	29.586922584211923	25.83266967201736
47	19.004629798758405	24.12166555853493	32.404499745495016	24.46920489721165
48	19.771456961868093	23.83647385174415	31.165584107198956	25.226485079188798
49	21.824057905363116	20.81275983253816	32.47386148596813	24.8893207761306
50	20.734679165418495	21.356986466180064	30.198562594830502	27.709771773570935
51	20.257427526966566	21.300386506608607	27.852197145160385	30.58998882126444
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	555.0
1	1207.0
2	1859.0
3	2723.5
4	3588.0
5	2703.0
6	1818.0
7	1934.0
8	2050.0
9	2448.0
10	2846.0
11	3056.0
12	3266.0
13	3303.5
14	3341.0
15	3349.0
16	3357.0
17	3729.5
18	4102.0
19	3863.0
20	3624.0
21	4471.5
22	5319.0
23	5767.5
24	6216.0
25	7983.0
26	13323.0
27	16896.0
28	18929.0
29	20962.0
30	24382.5
31	27803.0
32	34058.0
33	40313.0
34	45319.5
35	50326.0
36	54074.0
37	57822.0
38	64611.5
39	71401.0
40	80829.5
41	90258.0
42	102175.0
43	114092.0
44	128546.0
45	143000.0
46	169595.5
47	196191.0
48	226204.0
49	256217.0
50	247302.5
51	238388.0
52	202911.5
53	167435.0
54	147604.0
55	127773.0
56	113052.0
57	98331.0
58	88256.0
59	78181.0
60	68935.0
61	59689.0
62	51733.0
63	43777.0
64	39872.0
65	35967.0
66	28920.0
67	21873.0
68	18482.0
69	15091.0
70	12976.0
71	10861.0
72	9066.0
73	7271.0
74	6324.0
75	4103.0
76	2829.0
77	2321.0
78	1813.0
79	1301.0
80	789.0
81	532.0
82	275.0
83	239.0
84	203.0
85	133.0
86	63.0
87	51.5
88	40.0
89	23.0
90	6.0
91	3.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2053005.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.233845753190344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.25986573683008	16.215235401238914
2	13.972554652920582	7.051625777796401
3	6.322725510634799	4.786400408253605
4	3.5302130990870983	3.5632341127302363
5	2.0988427942190153	2.6480937664758826
6	1.4082325877990405	2.132107434308224
7	0.96558615135634	1.7055816403319812
8	0.7147031653259546	1.4427767546521628
9	0.5333642974767168	1.211294917150758
>10	4.885968380219972	29.091859274385644
>50	1.1632300642824267	19.314260855782045
>100	0.12960045233802486	5.687107623645309
>500	0.010558472370047063	1.7817955784927026
>1k	0.00372651966001661	1.599003109816303
>5k	8.281154800036912E-4	1.7696233449398013
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC	9824	0.47851807472461094	No Hit
CTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGCT	8536	0.4157807701393811	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC	8294	0.403993170985945	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC	7220	0.35167961110664614	No Hit
TCCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTG	3408	0.16600056989632272	No Hit
GCCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTG	2438	0.11875275510775667	No Hit
CGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTG	2239	0.109059646712989	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCC	2236	0.10891351945075632	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	2101	0.1023377926502858	No Hit
TTCCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCT	2080	0.10131490181465705	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.42839642377880227	0.0
2	0.0	0.0	0.0	1.7382812024325318	0.0
3	0.0	0.0	0.0	2.5245920005065745	0.0
4	0.0	0.0	0.0	3.3790955209558673	0.0
5	0.0	0.0	0.0	4.896919393766698	0.0
6	0.0	0.0	0.0	5.906999739406382	0.0
7	0.0	0.0	0.0	6.808848492819063	0.0
8	0.0	0.0	0.0	8.207870901434726	0.0
9	0.0	0.0	0.0	8.819949293840006	0.0
10	0.0	0.0	0.0	10.120969018584953	0.0
11	0.0	0.0	0.0	12.47064668619901	0.0
12	0.0	0.0	0.0	14.4663067065107	0.0
13	4.870908741089281E-5	0.0	0.0	15.127581277200981	0.0
14	4.870908741089281E-5	0.0	0.0	15.374974732160906	0.0
15	4.870908741089281E-5	0.0	0.0	15.74404348747324	0.0
16	4.870908741089281E-5	0.0	0.0	16.60975009802704	0.0
17	9.741817482178562E-5	0.0	0.0	17.813205520687966	0.0
18	3.409636118762497E-4	0.0	0.0	19.136777552904157	0.0
19	3.409636118762497E-4	0.0	0.0	20.03268379765271	0.0
20	3.409636118762497E-4	0.0	0.0	20.979150075133767	0.0
21	3.409636118762497E-4	0.0	0.0	22.281631072501042	0.0
22	3.409636118762497E-4	0.0	0.0	23.56107267152296	0.0
23	3.409636118762497E-4	0.0	0.0	24.804956636734932	0.0
24	3.409636118762497E-4	0.0	0.0	25.770760421918116	0.0
25	3.896726992871425E-4	0.0	0.0	26.633788032664313	0.0
26	4.3838178669803533E-4	0.0	0.0	27.447716883300334	0.0
27	4.3838178669803533E-4	0.0	0.0	28.300515585690246	0.0
28	4.3838178669803533E-4	0.0	0.0	29.10499487336855	0.0
29	4.3838178669803533E-4	0.0	0.0	30.01000971746294	0.0
30	4.870908741089281E-4	0.0	0.0	30.98117150226132	0.0
31	4.870908741089281E-4	0.0	0.0	31.78808624431017	0.0
32	4.870908741089281E-4	0.0	0.0	32.542882262829366	0.0
33	4.870908741089281E-4	0.0	0.0	33.3211073523932	0.0
34	4.870908741089281E-4	0.0	0.0	34.147944111193105	0.0
35	4.870908741089281E-4	0.0	0.0	35.03527755655734	0.0
36	5.357999615198209E-4	0.0	0.0	35.79898733807273	0.0
37	5.357999615198209E-4	0.0	0.0	36.5871977905558	0.0
38	5.357999615198209E-4	0.0	0.0	37.45782401893809	0.0
39	5.357999615198209E-4	0.0	0.0	38.761376616228404	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAATT	35	1.2125565E-7	45.0	37
ACGCATT	20	7.034585E-4	45.0	21
TAACGCG	20	7.034585E-4	45.0	43
CACTCGA	20	7.034585E-4	45.0	18
GTCGTAG	40	6.8193913E-9	45.0	1
CGTCGAC	20	7.034585E-4	45.0	29
TACGGGT	205	0.0	43.90244	4
TTACGGG	1085	0.0	43.75576	3
CTACGAA	150	0.0	43.500004	11
CTACGGG	555	0.0	42.972973	3
TACGGGA	535	0.0	42.897194	4
TTTACGG	730	0.0	42.53425	2
TCTACGG	430	0.0	41.860466	2
TACCGTA	65	0.0	41.53846	18
CCGTACA	55	6.184564E-11	40.909092	20
CAACCCG	150	0.0	40.500004	23
GCGGATA	50	1.0822987E-9	40.5	5
TACGGGG	1040	0.0	40.45673	4
TTATGGG	4070	0.0	40.356266	3
TGTTACG	190	0.0	40.263157	1
>>END_MODULE