FastQCFastQC Report
Sat 14 Jan 2017
SRR2935262.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935262.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3629117
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGCT55600.15320531137464016No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG55030.15163468138392894No Hit
CCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC52760.1453797163332017No Hit
GCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC46520.1281854511717313No Hit
TCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC42590.11735637070945909No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37240.10261449272646761No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACG207.035301E-445.029
AATCGTC253.8924765E-545.012
TACGGGA15300.042.2058834
TTACGGG27600.041.6576083
TATGGGA41400.041.6304364
TTATGGG72950.041.267993
TAACGGG2100.039.6428573
CTTTACG3350.039.6268651
TTTATGG45650.039.381162
TTTACGG16500.039.1363642
TTTTACG9750.038.769231
TTTGGGC79800.038.7687954
TGTACGG5200.038.5096172
TACGGGG26500.038.3773574
CTATGGG39600.038.1253
TATGGGC22950.037.843144
AGACACG16700.037.7245524
CGACCAA16600.037.68072529
ATGGGCG10050.037.611945
TATGGGG72800.037.551514
Produced by FastQC (version 0.11.5)