##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935262.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3629117
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45727293994655	33.0	31.0	34.0	31.0	34.0
2	32.60426241424567	34.0	31.0	34.0	31.0	34.0
3	32.660997427197856	34.0	31.0	34.0	31.0	34.0
4	36.06407068165617	37.0	35.0	37.0	35.0	37.0
5	31.6901918565866	37.0	35.0	37.0	0.0	37.0
6	33.466812726070835	37.0	35.0	37.0	17.0	37.0
7	35.282589676772616	37.0	35.0	37.0	32.0	37.0
8	35.844488618030226	37.0	35.0	37.0	35.0	37.0
9	37.908625982573724	39.0	38.0	39.0	35.0	39.0
10	37.65820501240384	39.0	37.0	39.0	35.0	39.0
11	37.522481363924065	39.0	37.0	39.0	35.0	39.0
12	37.16868125221645	39.0	37.0	39.0	34.0	39.0
13	37.04597233982812	39.0	37.0	39.0	33.0	39.0
14	38.007532135227386	40.0	37.0	41.0	33.0	41.0
15	38.17847426798309	40.0	37.0	41.0	33.0	41.0
16	38.330677682753134	40.0	37.0	41.0	34.0	41.0
17	38.350910428073824	40.0	37.0	41.0	34.0	41.0
18	38.34593924637866	40.0	37.0	41.0	34.0	41.0
19	38.35688212862798	40.0	37.0	41.0	34.0	41.0
20	38.27638486166194	40.0	37.0	41.0	34.0	41.0
21	38.22909016160129	40.0	37.0	41.0	34.0	41.0
22	38.243953556746725	40.0	37.0	41.0	34.0	41.0
23	38.21028779176863	40.0	36.0	41.0	34.0	41.0
24	38.18415361091968	40.0	36.0	41.0	34.0	41.0
25	38.08052096419046	40.0	36.0	41.0	34.0	41.0
26	38.03222629636906	40.0	36.0	41.0	34.0	41.0
27	37.98805880328466	40.0	36.0	41.0	34.0	41.0
28	37.95717746217606	40.0	36.0	41.0	34.0	41.0
29	37.88200876411535	40.0	36.0	41.0	34.0	41.0
30	37.8136329029899	40.0	36.0	41.0	34.0	41.0
31	37.58364941113775	40.0	35.0	41.0	33.0	41.0
32	37.611218100711554	40.0	35.0	41.0	33.0	41.0
33	37.61243602782716	40.0	35.0	41.0	33.0	41.0
34	37.50669047043675	40.0	35.0	41.0	33.0	41.0
35	37.410942937359145	40.0	35.0	41.0	33.0	41.0
36	37.3775979115581	39.0	35.0	41.0	33.0	41.0
37	37.34808329409054	39.0	35.0	41.0	33.0	41.0
38	37.31010463426779	39.0	35.0	41.0	33.0	41.0
39	37.3061011810862	39.0	35.0	41.0	33.0	41.0
40	37.20366689748498	39.0	35.0	41.0	33.0	41.0
41	37.09854353001019	39.0	35.0	41.0	33.0	41.0
42	37.092196256003874	39.0	35.0	41.0	33.0	41.0
43	37.066734139461474	39.0	35.0	41.0	33.0	41.0
44	37.022442649272534	39.0	35.0	41.0	33.0	41.0
45	36.96921592773118	39.0	35.0	41.0	33.0	41.0
46	36.88398031807737	39.0	35.0	41.0	33.0	41.0
47	36.824851609909516	38.0	35.0	41.0	33.0	41.0
48	36.76954311475767	38.0	35.0	41.0	32.0	41.0
49	36.72498682186328	38.0	35.0	41.0	32.0	41.0
50	36.644734793615086	38.0	35.0	40.0	32.0	41.0
51	36.01105227525043	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	14.0
9	24.0
10	24.0
11	40.0
12	35.0
13	61.0
14	68.0
15	106.0
16	168.0
17	315.0
18	526.0
19	909.0
20	1434.0
21	2479.0
22	3876.0
23	5887.0
24	8686.0
25	12696.0
26	17491.0
27	22436.0
28	27721.0
29	35024.0
30	45884.0
31	62167.0
32	89139.0
33	144596.0
34	264074.0
35	302008.0
36	294347.0
37	462979.0
38	719136.0
39	1103456.0
40	1310.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.021678827108634	4.374232079042919	54.17273678418194	16.43135230966651
2	19.983015152170623	4.258418783412053	53.27516307685865	22.48340298755868
3	21.898026434529392	4.610019462034429	50.255337593139046	23.236616510297132
4	19.199050347508777	5.072115338248946	50.01340546474529	25.715428849496995
5	16.75195371215643	17.597558855225664	43.30822070492629	22.342266727691612
6	22.416527215848923	6.799890992767662	51.33044759923695	19.453134192146464
7	85.3779583298086	2.6235858474664773	7.682915706492792	4.31554011623213
8	88.23961861797235	2.6194526106488163	5.412804271672696	3.7281244997061274
9	83.55622042496839	4.696404111523547	7.310097745539755	4.4372777179683105
10	44.668413831794346	24.911514288461902	14.765051664082476	15.655020215661274
11	34.648814022805	24.535307073318386	21.739282585819083	19.076596318057533
12	29.747539139686047	22.27167104284596	26.975046547135296	21.0057432703327
13	27.60081860132919	22.047070954174252	29.335207434756168	21.016903009740385
14	22.491834790666708	23.40483924877594	32.45742697190529	21.64589898865206
15	21.232547751973826	22.377729899587145	33.85616942082606	22.53355292761297
16	26.855485783456416	22.479848403895495	29.16282390454758	21.50184190810051
17	27.1201231594352	22.420798227227174	27.46998787859416	22.989090734743463
18	28.191706136782035	21.651575300548316	28.35957066140331	21.797147901266342
19	27.650224558756303	23.95307178027052	26.687869253044198	21.708834407928983
20	27.458249486032	27.127480320970637	26.11392798854377	19.300342204453592
21	26.479664337082546	25.63557471417979	28.289388300239427	19.595372648498245
22	25.619840859360554	21.813570628888517	29.857565903772187	22.709022607978746
23	23.922844041677354	23.952024693610046	29.443195135345597	22.681936129367006
24	23.972525548225644	25.620860391108913	28.2157340201487	22.190880040516742
25	25.97634631233989	24.926614380302425	25.220625292598726	23.87641401475896
26	22.584253965909614	25.278711047342924	26.550342686664553	25.586692300082913
27	22.892262773561722	23.835054091670234	29.13243083648171	24.14025229828633
28	20.794286874741154	25.63083526929553	27.985016740986858	25.589861114976454
29	23.173488206635387	25.492702494849297	27.70111848143777	23.632690817077542
30	26.05628311239346	23.287813537011896	28.485937488375274	22.169965862219378
31	26.09047875833157	24.954665280838288	26.687924362868433	22.266931597961708
32	27.04404404707812	24.56867607189297	26.203977441344545	22.183302439684365
33	27.72635878093762	23.6966182131907	25.560625353219528	23.016397652652145
34	22.927836165105727	24.772472201915782	29.234935109559707	23.06475652341878
35	23.336282627427003	26.33177712374663	28.17883799282305	22.153102256003322
36	26.231367023989584	26.848900159460275	25.986376300350745	20.933356516199396
37	24.311506077098095	27.92494703257018	25.29190985024732	22.471637040084406
38	25.659464822985868	26.81310632861933	25.011345735064477	22.516083113330325
39	25.187090964551434	23.523049821761052	25.86248941546938	25.427369798218137
40	25.398547360142977	23.354579089073184	27.1598848976211	24.086988653162738
41	22.022051093971342	24.553851529173627	26.97634162800483	26.447755748850206
42	22.360563189337793	24.373559739187243	28.716434328240176	24.54944274323479
43	22.803177742685065	23.250697070389297	29.293957731315913	24.652167455609725
44	23.969191403859398	23.046515171596837	28.07096051188209	24.913332912661676
45	23.86597070306634	22.60588457192204	26.628019983924467	26.900124741087154
46	23.15629394147392	23.373095990016303	27.84834437688286	25.622265691626918
47	21.397519010822744	25.337210125768884	29.858227221663007	23.40704364174536
48	22.226012553466862	26.341779556845367	28.45951232765436	22.97269556203341
49	25.114098002351536	23.603510165144854	28.270017197020653	23.012374635482956
50	22.91681420025863	24.322803591066368	26.762047076465155	25.99833513220985
51	22.172886682903858	24.59220796684152	26.146277455369997	27.088627894884628
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	682.0
1	1799.5
2	2917.0
3	5753.0
4	8589.0
5	6111.0
6	3633.0
7	3662.0
8	3691.0
9	4077.5
10	4464.0
11	4566.5
12	4669.0
13	4643.5
14	4618.0
15	4855.0
16	5092.0
17	5321.0
18	5550.0
19	6267.0
20	6984.0
21	7942.0
22	8900.0
23	10768.5
24	12637.0
25	15790.5
26	23197.0
27	27450.0
28	32180.5
29	36911.0
30	44548.5
31	52186.0
32	61304.0
33	70422.0
34	78050.0
35	85678.0
36	93770.0
37	101862.0
38	110377.0
39	118892.0
40	132253.0
41	145614.0
42	158786.5
43	171959.0
44	194302.0
45	216645.0
46	240258.5
47	263872.0
48	295141.0
49	326410.0
50	327796.0
51	329182.0
52	307998.0
53	286814.0
54	268131.5
55	249449.0
56	237732.5
57	226016.0
58	212051.0
59	198086.0
60	180790.0
61	163494.0
62	147135.5
63	130777.0
64	120059.5
65	109342.0
66	91174.0
67	73006.0
68	62953.0
69	52900.0
70	44991.0
71	37082.0
72	30910.5
73	24739.0
74	21089.5
75	14249.0
76	11058.0
77	8457.0
78	5856.0
79	4218.5
80	2581.0
81	1858.5
82	1136.0
83	793.0
84	450.0
85	320.5
86	191.0
87	153.5
88	116.0
89	116.5
90	117.0
91	63.5
92	10.0
93	6.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3629117.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.104490839014602
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.17953263350176	14.388943218720351
2	12.508108134944276	5.279545070946711
3	5.247220349878832	3.3221974141292647
4	2.740177603242242	2.3132021249959553
5	1.6437680042367102	1.7345443393439488
6	1.0881623691746638	1.3779067650964272
7	0.7529865869989099	1.1123978969053556
8	0.5546273403960429	0.9364102099564259
9	0.4071847170900909	0.7734083518453118
>10	4.290062784267112	22.189997706138517
>50	2.2143422571302076	33.3639352071288
>100	0.36303298690459174	10.59187027016991
>500	0.007811615432971019	1.1228775802503077
>1k	0.002556528687154147	1.0030092520310654
>5k	4.2608811452569115E-4	0.48975459234162866
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGCT	5560	0.15320531137464016	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	5503	0.15163468138392894	No Hit
CCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC	5276	0.1453797163332017	No Hit
GCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC	4652	0.1281854511717313	No Hit
TCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC	4259	0.11735637070945909	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3724	0.10261449272646761	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7554912117741038E-5	0.0	0.0	0.1587438487103061	0.0
2	2.7554912117741038E-5	0.0	0.0	0.5767794204485553	0.0
3	2.7554912117741038E-5	0.0	0.0	0.8245531902112828	0.0
4	2.7554912117741038E-5	0.0	0.0	1.1481305232099157	0.0
5	2.7554912117741038E-5	0.0	0.0	1.737061659902395	0.0
6	2.7554912117741038E-5	0.0	0.0	2.267383498520439	0.0
7	2.7554912117741038E-5	0.0	0.0	2.732565524892143	0.0
8	2.7554912117741038E-5	0.0	0.0	3.445493766114457	0.0
9	2.7554912117741038E-5	0.0	0.0	3.8178708484736092	0.0
10	2.7554912117741038E-5	0.0	0.0	4.47130803443372	0.0
11	2.7554912117741038E-5	0.0	0.0	5.724836096493996	0.0
12	2.7554912117741038E-5	0.0	0.0	6.797907039095185	0.0
13	2.7554912117741038E-5	0.0	0.0	7.1408554753125895	0.0
14	2.7554912117741038E-5	0.0	0.0	7.273229273126217	0.0
15	2.7554912117741038E-5	0.0	0.0	7.464295033750634	0.0
16	5.5109824235482076E-5	0.0	0.0	7.894261882435865	0.0
17	5.5109824235482076E-5	0.0	0.0	8.490770619960724	0.0
18	8.266473635322311E-5	0.0	0.0	9.120124812729928	0.0
19	8.266473635322311E-5	0.0	0.0	9.586546810146931	0.0
20	8.266473635322311E-5	0.0	0.0	10.113672278959317	0.0
21	8.266473635322311E-5	0.0	0.0	10.860134848228922	0.0
22	8.266473635322311E-5	0.0	0.0	11.642611687636414	0.0
23	8.266473635322311E-5	0.0	0.0	12.366203679848294	0.0
24	1.1021964847096415E-4	0.0	0.0	12.964613706309276	0.0
25	1.1021964847096415E-4	0.0	0.0	13.487661048128237	0.0
26	1.377745605887052E-4	0.0	0.0	13.968136050725287	0.0
27	1.377745605887052E-4	0.0	0.0	14.499890744773452	0.0
28	1.377745605887052E-4	0.0	0.0	14.997670232180445	0.0
29	1.377745605887052E-4	0.0	0.0	15.59872553020473	0.0
30	1.377745605887052E-4	0.0	0.0	16.21973058460226	0.0
31	1.377745605887052E-4	0.0	0.0	16.744872099742167	0.0
32	1.377745605887052E-4	0.0	0.0	17.267864331736895	0.0
33	1.377745605887052E-4	0.0	0.0	17.809042805729327	0.0
34	1.377745605887052E-4	0.0	0.0	18.38122055585422	0.0
35	1.377745605887052E-4	0.0	0.0	18.99963544851268	0.0
36	1.377745605887052E-4	0.0	0.0	19.540069939877938	0.0
37	1.377745605887052E-4	0.0	0.0	20.114975626302485	0.0
38	1.377745605887052E-4	0.0	0.0	20.82925405821857	0.0
39	1.6532947270644623E-4	0.0	0.0	22.110199257835998	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAACG	20	7.035301E-4	45.0	29
AATCGTC	25	3.8924765E-5	45.0	12
TACGGGA	1530	0.0	42.205883	4
TTACGGG	2760	0.0	41.657608	3
TATGGGA	4140	0.0	41.630436	4
TTATGGG	7295	0.0	41.26799	3
TAACGGG	210	0.0	39.642857	3
CTTTACG	335	0.0	39.626865	1
TTTATGG	4565	0.0	39.38116	2
TTTACGG	1650	0.0	39.136364	2
TTTTACG	975	0.0	38.76923	1
TTTGGGC	7980	0.0	38.768795	4
TGTACGG	520	0.0	38.509617	2
TACGGGG	2650	0.0	38.377357	4
CTATGGG	3960	0.0	38.125	3
TATGGGC	2295	0.0	37.84314	4
AGACACG	1670	0.0	37.72455	24
CGACCAA	1660	0.0	37.680725	29
ATGGGCG	1005	0.0	37.61194	5
TATGGGG	7280	0.0	37.55151	4
>>END_MODULE