SRR2935261.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935261.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	619363
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	5818	0.9393522054110433	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGCT	5484	0.8854258326700174	TruSeq Adapter, Index 19 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	5158	0.8327911095754832	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	4628	0.7472193204954123	TruSeq Adapter, Index 13 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTG	2040	0.3293706598553675	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGC	1552	0.2505800314193776	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTC	1378	0.22248665160818454	No Hit
GCCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTG	1312	0.21183054202462853	No Hit
TTCCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT	1295	0.2090857865258338	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCG	1274	0.20569520620379325	No Hit
TGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGCTT	1221	0.19713802729578617	TruSeq Adapter, Index 13 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTG	1086	0.17534143951123976	No Hit
TTCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTG	942	0.1520917458743903	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCC	840	0.1356232128816219	No Hit
TTTCCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTC	780	0.12593584053293463	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTG	762	0.12302962882832846	No Hit
TCTGACTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	757	0.1222223477992712	TruSeq Adapter, Index 13 (95% over 21bp)
TGTTTCGGGGTTGTCCCAGACACTTGGAAAACTTACTGGGAAGATGTCTTG	733	0.11834739885979628	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTC	721	0.11640992439005882	No Hit
GCGTTTGGGGTTATGGGCTATTACATCTCTAAGGAATTGGTGAAGTAAAAT	658	0.10623818342393716	No Hit
GGCCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT	656	0.10591527101231427	No Hit
TGCCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT	627	0.10123304104378207	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATGTAC	35	1.2107193E-7	45.000004	9
CAAAACG	30	2.1638734E-6	45.000004	15
CGTAAGG	30	2.1638734E-6	45.000004	2
CGATGAA	35	1.2107193E-7	45.000004	19
CTTGTAT	20	7.0307637E-4	45.0	41
TTCTACG	45	3.8380676E-10	45.0	1
CGAAAAG	20	7.0307637E-4	45.0	21
CTATCGC	20	7.0307637E-4	45.0	34
GCGCCCA	20	7.0307637E-4	45.0	19
GTCTACG	20	7.0307637E-4	45.0	1
ACGGGTA	90	0.0	45.0	5
ACGGGAG	110	0.0	45.0	5
TGGCACG	20	7.0307637E-4	45.0	32
TGACACT	20	7.0307637E-4	45.0	13
TTGACGT	20	7.0307637E-4	45.0	23
AGACATA	40	6.8066583E-9	45.0	43
TCGTAAG	20	7.0307637E-4	45.0	1
CCGACTC	45	3.8380676E-10	45.0	27
TCTCCCG	45	3.8380676E-10	45.0	1
TTAGTCA	45	3.8380676E-10	45.0	32

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content