##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935261.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	619363
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07566160716736	33.0	31.0	34.0	30.0	34.0
2	32.22988296039641	34.0	31.0	34.0	30.0	34.0
3	32.29739910198058	34.0	31.0	34.0	30.0	34.0
4	35.79605982275338	37.0	35.0	37.0	35.0	37.0
5	31.603503922578522	37.0	35.0	37.0	0.0	37.0
6	33.315945576342145	37.0	35.0	37.0	17.0	37.0
7	35.102671615837565	37.0	35.0	37.0	32.0	37.0
8	35.63934397114455	37.0	35.0	37.0	35.0	37.0
9	37.63010544704802	39.0	37.0	39.0	35.0	39.0
10	37.36924549900462	39.0	37.0	39.0	34.0	39.0
11	37.261610719400416	39.0	37.0	39.0	34.0	39.0
12	36.975066318136534	39.0	35.0	39.0	33.0	39.0
13	36.76648427497283	39.0	35.0	39.0	33.0	39.0
14	37.757807618472526	40.0	36.0	41.0	33.0	41.0
15	37.92270284146777	40.0	36.0	41.0	33.0	41.0
16	38.057199090032825	40.0	36.0	41.0	34.0	41.0
17	38.09948931402102	40.0	36.0	41.0	34.0	41.0
18	38.119794046463866	40.0	36.0	41.0	34.0	41.0
19	38.14801013299148	40.0	36.0	41.0	34.0	41.0
20	38.0324720721128	40.0	36.0	41.0	34.0	41.0
21	37.953996283278144	40.0	36.0	41.0	34.0	41.0
22	37.9073289815504	40.0	36.0	41.0	34.0	41.0
23	37.879850749883346	39.0	36.0	41.0	34.0	41.0
24	37.856245852593716	39.0	36.0	41.0	34.0	41.0
25	37.72885690620848	39.0	36.0	41.0	33.0	41.0
26	37.690649909665254	39.0	35.0	41.0	33.0	41.0
27	37.65732696334783	39.0	35.0	41.0	33.0	41.0
28	37.62862166451661	39.0	36.0	41.0	33.0	41.0
29	37.5727093804441	39.0	36.0	41.0	33.0	41.0
30	37.57198767120413	39.0	36.0	41.0	33.0	41.0
31	37.336925195725286	39.0	35.0	41.0	33.0	41.0
32	37.36834941706237	39.0	35.0	41.0	33.0	41.0
33	37.40293172178512	39.0	35.0	41.0	33.0	41.0
34	37.38258501072877	39.0	35.0	41.0	33.0	41.0
35	37.29902012228693	39.0	35.0	41.0	33.0	41.0
36	37.305027907705174	39.0	35.0	41.0	33.0	41.0
37	37.22108036805557	39.0	35.0	41.0	33.0	41.0
38	37.1926350137157	39.0	35.0	41.0	33.0	41.0
39	37.18405361637683	39.0	35.0	41.0	33.0	41.0
40	37.1502834363693	39.0	35.0	41.0	33.0	41.0
41	37.11655039128912	39.0	35.0	41.0	33.0	41.0
42	37.108844086585734	39.0	35.0	41.0	33.0	41.0
43	36.99612666562258	39.0	35.0	41.0	32.0	41.0
44	36.864166894050825	39.0	35.0	40.0	32.0	41.0
45	36.8907458146515	39.0	35.0	40.0	32.0	41.0
46	36.80729071642962	39.0	35.0	40.0	32.0	41.0
47	36.75208238141445	39.0	35.0	40.0	32.0	41.0
48	36.74402248762035	39.0	35.0	40.0	32.0	41.0
49	36.76667317873363	39.0	35.0	40.0	32.0	41.0
50	36.679370579127266	39.0	35.0	40.0	31.0	41.0
51	35.9956826610566	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	3.0
11	9.0
12	6.0
13	6.0
14	11.0
15	24.0
16	22.0
17	31.0
18	68.0
19	122.0
20	225.0
21	422.0
22	622.0
23	1072.0
24	1598.0
25	2261.0
26	3054.0
27	3939.0
28	5011.0
29	6575.0
30	8839.0
31	12009.0
32	16859.0
33	25351.0
34	42106.0
35	63913.0
36	54621.0
37	83108.0
38	130699.0
39	156666.0
40	109.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.08862008224579	4.451509050427617	55.36365588515943	16.09621498216716
2	19.27593349941795	5.890729669030923	52.06203793252099	22.771298899030132
3	19.875258935390068	5.134953169627504	52.596457973756905	22.393329921225515
4	18.531620390627143	4.6044080773310645	52.565458382241104	24.298513149800684
5	15.261970766739374	17.17038311943077	45.02819832634497	22.539447787484885
6	19.615152987827816	6.994121379546405	54.043751402650784	19.34697422997499
7	77.0867810960616	2.258126494479005	13.510009477479281	7.145082931980115
8	76.75870208585273	3.5704425353145086	10.492715903274815	9.178139475557952
9	70.72766697397165	6.169725992673118	14.161162355516879	8.94144467783836
10	38.57673125453086	24.900260428859973	20.48217281303533	16.040835503573835
11	29.431044476341018	24.167895079299214	26.742475737168668	19.6585847071911
12	25.827826331246783	19.314682988812702	34.13232627715895	20.72516440278157
13	22.05071985249361	27.762556045485443	33.18425543663409	17.002468665386857
14	16.968885774578073	30.935170489680523	31.88792356017392	20.20802017556748
15	12.965579151483055	24.89460946165657	40.998574341702685	21.141237045157688
16	13.972097138511664	23.91295573032293	36.84382179755652	25.271125333608886
17	14.40786743799678	23.39791043378439	30.565597234578107	31.628624893640723
18	16.009835912058033	22.536864488191902	35.596249695251416	25.857049904498652
19	20.14844283562305	23.879534295719957	32.29947542878732	23.672547439869675
20	22.048298009406437	25.422894167071654	31.28811375558437	21.240694067937543
21	19.926763465043923	27.48129933496189	30.675064542118275	21.916872657875913
22	17.80942678203251	26.13184836678975	28.158285205929317	27.900439645248426
23	17.400619668917905	27.336150205937393	26.870187595965533	28.393042529179173
24	20.344935038095592	22.912573079115155	30.134509165061523	26.60798271772773
25	17.477472822884156	26.374517044124367	28.63361227583824	27.514397857153238
26	18.46219422212822	26.74021535028731	28.601966859499196	26.195623568085274
27	19.488409866265823	26.702596054333245	28.42856289445769	25.38043118494324
28	15.729709394975162	24.57670219241382	35.220541104328156	24.473047308282865
29	17.643611258664144	23.980444424352115	31.764893931345593	26.611050385638148
30	19.727042138455158	25.348947224810008	29.824028881286097	25.09998175544874
31	20.76552845423443	27.408159673729298	25.898382693186388	25.927929178849883
32	21.014816836007316	28.087728843989712	26.601685925701084	24.295768394301888
33	21.949163899038204	23.72453633814096	27.714603552359442	26.611696210461393
34	19.413171274357687	21.718120068522015	30.70154335987135	28.167165297248946
35	19.426895051851663	22.461787352489573	28.64055489268813	29.47076270297063
36	24.587196845791564	21.620439064006085	29.92784522162286	23.86451886857949
37	18.28443093952981	23.9139244675578	33.10304296511093	24.698601627801466
38	17.404978986474813	27.651312719681346	31.48702780114408	23.456680492699757
39	20.703690727408645	24.467396341079464	31.4647468447421	23.364166086769796
40	22.5100627580272	20.296175263940533	31.701280186255882	25.492481791776388
41	19.49099316555881	21.120086282196386	28.488947515431178	30.899973036813627
42	24.727502288641716	19.306610178522128	29.174490565306616	26.79139696752954
43	25.147934248574742	20.312482340727488	28.968795359102824	25.570788051594946
44	20.004262443833422	22.34279412880653	30.380406966512368	27.27253646084768
45	19.909972019639532	22.386225848169815	27.789196319444333	29.91460581274632
46	22.534604101310542	21.422170843269615	31.955412254203107	24.087812801216735
47	17.52235764809974	21.3613018536787	35.26413427989725	25.85220621832431
48	18.951729438148547	20.121156736840913	33.96231289243949	26.96480093257104
49	21.899596843854088	17.32312069012841	36.33975552301316	24.437526943004347
50	21.499669822059115	18.069209817183136	31.7439046245901	28.687215736167644
51	18.64302517263705	18.396481546362956	28.79070916409279	34.16978411690721
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	183.0
1	302.5
2	422.0
3	729.0
4	1036.0
5	786.0
6	536.0
7	694.5
8	853.0
9	1164.5
10	1476.0
11	1512.5
12	1549.0
13	1581.5
14	1614.0
15	1761.0
16	1908.0
17	1670.5
18	1433.0
19	1705.5
20	1978.0
21	1794.5
22	1611.0
23	1709.5
24	1808.0
25	1937.0
26	2694.0
27	3322.0
28	4475.5
29	5629.0
30	6295.0
31	6961.0
32	7527.0
33	8093.0
34	9439.0
35	10785.0
36	11529.5
37	12274.0
38	15387.5
39	18501.0
40	23524.0
41	28547.0
42	36440.0
43	44333.0
44	51613.0
45	58893.0
46	72492.5
47	86092.0
48	93442.0
49	100792.0
50	90876.0
51	80960.0
52	62567.5
53	44175.0
54	35107.0
55	26039.0
56	22310.0
57	18581.0
58	15702.0
59	12823.0
60	11254.0
61	9685.0
62	8746.5
63	7808.0
64	6589.5
65	5371.0
66	4340.0
67	3309.0
68	2921.5
69	2534.0
70	2279.0
71	2024.0
72	1732.0
73	1440.0
74	1182.5
75	701.5
76	478.0
77	367.5
78	257.0
79	197.5
80	138.0
81	111.5
82	85.0
83	53.0
84	21.0
85	12.5
86	4.0
87	7.5
88	11.0
89	5.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	619363.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.509404306777853
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.22012077267422	18.593867920450506
2	13.285826600617131	7.575420042134756
3	6.180538463043103	5.286104096294616
4	3.556615861028176	4.055879981838045
5	2.211928775109281	3.1530385873693203
6	1.5960828382206076	2.7302022565164488
7	1.1104190390731679	2.216016973441649
8	0.8490407778325705	1.936451744653592
9	0.6164489665626078	1.5817133540005892
>10	4.711359785861124	29.322600713097735
>50	0.5135796421860845	9.490256083001283
>100	0.13038773620910085	6.805051236856318
>500	0.010818196454030203	2.019016919135988
>1k	0.005124408846645885	2.562486017485183
>5k	0.0017081362822152953	2.67189407372394
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	5818	0.9393522054110433	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGCT	5484	0.8854258326700174	TruSeq Adapter, Index 19 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	5158	0.8327911095754832	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	4628	0.7472193204954123	TruSeq Adapter, Index 13 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTG	2040	0.3293706598553675	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGC	1552	0.2505800314193776	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTC	1378	0.22248665160818454	No Hit
GCCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTG	1312	0.21183054202462853	No Hit
TTCCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT	1295	0.2090857865258338	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCG	1274	0.20569520620379325	No Hit
TGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGCTT	1221	0.19713802729578617	TruSeq Adapter, Index 13 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTG	1086	0.17534143951123976	No Hit
TTCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTG	942	0.1520917458743903	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCC	840	0.1356232128816219	No Hit
TTTCCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTC	780	0.12593584053293463	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTG	762	0.12302962882832846	No Hit
TCTGACTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	757	0.1222223477992712	TruSeq Adapter, Index 13 (95% over 21bp)
TGTTTCGGGGTTGTCCCAGACACTTGGAAAACTTACTGGGAAGATGTCTTG	733	0.11834739885979628	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTC	721	0.11640992439005882	No Hit
GCGTTTGGGGTTATGGGCTATTACATCTCTAAGGAATTGGTGAAGTAAAAT	658	0.10623818342393716	No Hit
GGCCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT	656	0.10591527101231427	No Hit
TGCCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT	627	0.10123304104378207	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9162639679800053	0.0
2	0.0	0.0	0.0	3.592723491716489	0.0
3	0.0	0.0	0.0	5.005949661184152	0.0
4	0.0	0.0	0.0	6.670401686894438	0.0
5	0.0	0.0	0.0	9.316023075320935	0.0
6	0.0	0.0	0.0	11.035531667212927	0.0
7	0.0	0.0	0.0	12.616995203136126	0.0
8	0.0	0.0	0.0	15.178659364540666	0.0
9	0.0	0.0	0.0	16.478543277528686	0.0
10	0.0	0.0	0.0	18.83289767067132	0.0
11	0.0	0.0	0.0	22.592405422991042	0.0
12	0.0	0.0	0.0	26.273122546874774	0.0
13	0.0	0.0	0.0	27.34163971693498	0.0
14	1.6145620581145466E-4	0.0	0.0	27.683442504637828	0.0
15	1.6145620581145466E-4	0.0	0.0	28.223674969282957	0.0
16	1.6145620581145466E-4	0.0	0.0	29.652723846920143	0.0
17	1.6145620581145466E-4	0.0	0.0	31.736800551534397	0.0
18	1.6145620581145466E-4	0.0	0.0	34.4163277431813	0.0
19	1.6145620581145466E-4	0.0	0.0	35.8285851754141	0.0
20	1.6145620581145466E-4	0.0	0.0	37.26409230128374	0.0
21	1.6145620581145466E-4	0.0	0.0	39.09129218245197	0.0
22	1.6145620581145466E-4	0.0	0.0	40.65515699194172	0.0
23	1.6145620581145466E-4	0.0	0.0	42.09453906675084	0.0
24	1.6145620581145466E-4	0.0	0.0	43.24475307695164	0.0
25	1.6145620581145466E-4	0.0	0.0	44.202672746031006	0.0
26	1.6145620581145466E-4	0.0	0.0	45.04289084107381	0.0
27	1.6145620581145466E-4	0.0	0.0	45.79834442806561	0.0
28	1.6145620581145466E-4	0.0	0.0	46.555735489527144	0.0
29	1.6145620581145466E-4	0.0	0.0	47.37286534713891	0.0
30	1.6145620581145466E-4	0.0	0.0	48.322227837310265	0.0
31	1.6145620581145466E-4	0.0	0.0	49.165513600263495	0.0
32	1.6145620581145466E-4	0.0	0.0	49.939211738511986	0.0
33	1.6145620581145466E-4	0.0	0.0	50.666733401898405	0.0
34	1.6145620581145466E-4	0.0	0.0	51.443983576674746	0.0
35	1.6145620581145466E-4	0.0	0.0	52.26967061319452	0.0
36	1.6145620581145466E-4	0.0	0.0	52.975880057413825	0.0
37	1.6145620581145466E-4	0.0	0.0	53.61621536966206	0.0
38	1.6145620581145466E-4	0.0	0.0	54.28932629169001	0.0
39	1.6145620581145466E-4	0.0	0.0	54.93676567699394	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATGTAC	35	1.2107193E-7	45.000004	9
CAAAACG	30	2.1638734E-6	45.000004	15
CGTAAGG	30	2.1638734E-6	45.000004	2
CGATGAA	35	1.2107193E-7	45.000004	19
CTTGTAT	20	7.0307637E-4	45.0	41
TTCTACG	45	3.8380676E-10	45.0	1
CGAAAAG	20	7.0307637E-4	45.0	21
CTATCGC	20	7.0307637E-4	45.0	34
GCGCCCA	20	7.0307637E-4	45.0	19
GTCTACG	20	7.0307637E-4	45.0	1
ACGGGTA	90	0.0	45.0	5
ACGGGAG	110	0.0	45.0	5
TGGCACG	20	7.0307637E-4	45.0	32
TGACACT	20	7.0307637E-4	45.0	13
TTGACGT	20	7.0307637E-4	45.0	23
AGACATA	40	6.8066583E-9	45.0	43
TCGTAAG	20	7.0307637E-4	45.0	1
CCGACTC	45	3.8380676E-10	45.0	27
TCTCCCG	45	3.8380676E-10	45.0	1
TTAGTCA	45	3.8380676E-10	45.0	32
>>END_MODULE