FastQCFastQC Report
Sat 14 Jan 2017
SRR2935260.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935260.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1058027
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC93820.8867448562276767Illumina PCR Primer Index 7 (96% over 27bp)
CTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGCT87250.8246481422496779Illumina PCR Primer Index 7 (96% over 28bp)
TCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC69140.6534804877380256Illumina PCR Primer Index 7 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC50100.473522887412136Illumina PCR Primer Index 7 (96% over 27bp)
TCCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTG26060.2463075138914224Illumina PCR Primer Index 7 (96% over 26bp)
TTCCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCT16330.15434388725429501Illumina PCR Primer Index 7 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTG15150.14319105278031657Illumina PCR Primer Index 7 (96% over 26bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCC13940.13175467166716917No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC13830.13071500065688305No Hit
TGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGCTT13820.1306204851104934Illumina PCR Primer Index 7 (96% over 29bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG13040.12324827249210087No Hit
TGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTG12570.11880604181178742Illumina PCR Primer Index 7 (96% over 26bp)
GAATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC12520.11833346407983916Illumina PCR Primer Index 7 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTG12080.11417478003869466Illumina PCR Primer Index 7 (96% over 26bp)
CGTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC11100.10491225649250917Illumina PCR Primer Index 7 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCACG351.2118107E-745.0000041
AGCCCGA351.2118107E-745.00000414
CGTCCCA302.1653377E-645.00000419
CAATACG302.1653377E-645.00000445
TACGTTA302.1653377E-645.00000427
AATCGTC302.1653377E-645.00000416
ACGTTAT253.890595E-545.028
ATTCGTC253.890595E-545.012
TACGGAT253.890595E-545.028
ATACTTA253.890595E-545.030
CCGTACA207.0330326E-445.031
CGGATTC253.890595E-545.014
CGTAGCA253.890595E-545.036
GAACGAC207.0330326E-445.021
CATACGT253.890595E-545.025
GCGATAT207.0330326E-445.09
TTAACGA253.890595E-545.032
ATACGTT253.890595E-545.026
CGCGTAC253.890595E-545.027
TATGTCG253.890595E-545.031
Produced by FastQC (version 0.11.5)