SRR2935259.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935259.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3320965
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14901	0.4486948823610005	No Hit
CTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGCT	9170	0.2761245601805499	TruSeq Adapter, Index 15 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC	8665	0.2609181367463975	No Hit
CCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC	8111	0.2442362385631887	No Hit
GCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC	7359	0.22159221792460929	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCC	4867	0.14655378782974227	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCT	4292	0.1292395433255093	No Hit
TCCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTG	4176	0.12574658269509015	TruSeq Adapter, Index 15 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTG	3930	0.1183390972202357	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTC	3901	0.1174658570626309	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTT	3883	0.11692384593032447	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGCG	30	2.1667456E-6	45.000004	1
TCTACGG	595	0.0	42.73109	2
CGTTTTT	10630	0.0	42.69285	1
TTTACGG	1565	0.0	42.26837	2
CGTTATT	1430	0.0	42.01049	1
CTACGGG	1075	0.0	41.23256	3
TTATGGG	7300	0.0	40.869865	3
CGTATGG	535	0.0	40.79439	2
TTACGGG	2430	0.0	40.74074	3
TACGGGC	765	0.0	40.588238	4
TATGGGA	4675	0.0	40.379677	4
TACGGGT	485	0.0	40.360825	4
TATGGGC	2340	0.0	40.192307	4
TACGGGA	1465	0.0	39.93174	4
TACGGGG	1800	0.0	39.875	4
GTACGGG	765	0.0	39.705883	3
TTTTACG	1100	0.0	39.68182	1
ATAATCG	40	3.4608456E-7	39.375004	33
ATGGGCG	1015	0.0	39.236458	5
GCGTATG	155	0.0	39.193546	1