##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935259.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3320965
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3375889237014	33.0	31.0	34.0	30.0	34.0
2	32.493908547666116	34.0	31.0	34.0	31.0	34.0
3	32.522675788513276	34.0	31.0	34.0	31.0	34.0
4	36.00343604946153	37.0	35.0	37.0	35.0	37.0
5	31.587670150091917	37.0	35.0	37.0	0.0	37.0
6	33.43097473174213	37.0	35.0	37.0	17.0	37.0
7	35.290165057445655	37.0	35.0	37.0	32.0	37.0
8	35.84696586684894	37.0	35.0	37.0	35.0	37.0
9	37.92126234392714	39.0	38.0	39.0	35.0	39.0
10	37.574689585707766	39.0	37.0	39.0	35.0	39.0
11	37.45080270343108	39.0	37.0	39.0	35.0	39.0
12	37.34741829558577	39.0	37.0	39.0	35.0	39.0
13	37.349641444580115	39.0	37.0	39.0	34.0	39.0
14	38.59670216337721	40.0	38.0	41.0	35.0	41.0
15	38.66750778764606	40.0	38.0	41.0	35.0	41.0
16	38.70349010001611	40.0	38.0	41.0	35.0	41.0
17	38.6835606517985	40.0	38.0	41.0	35.0	41.0
18	38.62010560183561	40.0	38.0	41.0	35.0	41.0
19	38.59019170632632	40.0	38.0	41.0	35.0	41.0
20	38.48253835857951	40.0	38.0	41.0	34.0	41.0
21	38.41796887350514	40.0	37.0	41.0	34.0	41.0
22	38.38442290117481	40.0	37.0	41.0	34.0	41.0
23	38.33527122387619	40.0	37.0	41.0	34.0	41.0
24	38.30857838007928	40.0	37.0	41.0	34.0	41.0
25	38.218332924315675	40.0	37.0	41.0	34.0	41.0
26	38.16128745710961	40.0	37.0	41.0	34.0	41.0
27	38.112258635667644	40.0	37.0	41.0	34.0	41.0
28	38.06417441918238	40.0	37.0	41.0	34.0	41.0
29	37.99877144143344	40.0	37.0	41.0	34.0	41.0
30	37.90711254108369	40.0	36.0	41.0	34.0	41.0
31	37.670550276802075	40.0	36.0	41.0	33.0	41.0
32	37.616752961865004	40.0	36.0	41.0	33.0	41.0
33	37.5205818188388	40.0	36.0	41.0	33.0	41.0
34	37.411845954413856	40.0	36.0	41.0	33.0	41.0
35	37.30499026638341	40.0	36.0	41.0	33.0	41.0
36	37.279730439796865	40.0	36.0	41.0	33.0	41.0
37	37.25037451463656	40.0	36.0	41.0	33.0	41.0
38	37.23621989391638	40.0	36.0	41.0	33.0	41.0
39	37.202051211018485	40.0	36.0	41.0	32.0	41.0
40	37.13398725972722	40.0	36.0	41.0	32.0	41.0
41	37.0784037169919	40.0	35.0	41.0	32.0	41.0
42	37.04466021171557	40.0	35.0	41.0	32.0	41.0
43	37.00807807369244	39.0	35.0	41.0	32.0	41.0
44	36.960503949906126	39.0	35.0	41.0	32.0	41.0
45	36.92884267072974	39.0	35.0	41.0	32.0	41.0
46	36.82840590009229	39.0	35.0	41.0	31.0	41.0
47	36.78716638085616	39.0	35.0	41.0	31.0	41.0
48	36.75052612719496	39.0	35.0	41.0	31.0	41.0
49	36.72231565222759	39.0	35.0	41.0	31.0	41.0
50	36.67105705721078	39.0	35.0	41.0	31.0	41.0
51	36.02612854998472	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	21.0
11	28.0
12	18.0
13	21.0
14	47.0
15	48.0
16	99.0
17	209.0
18	449.0
19	773.0
20	1425.0
21	2262.0
22	4096.0
23	6857.0
24	11656.0
25	19599.0
26	27102.0
27	29789.0
28	30525.0
29	33594.0
30	40696.0
31	52616.0
32	70886.0
33	104102.0
34	169753.0
35	240586.0
36	274627.0
37	436642.0
38	711693.0
39	1049619.0
40	1117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.291924184687282	4.1725221434131345	56.87518537533518	16.6603682965644
2	21.864427959945377	4.0152184681259815	52.96358136866844	21.156772203260196
3	18.711428756400625	4.038946511029174	54.55576918154813	22.693855551022068
4	17.62240794467873	4.384659278251954	53.77632706156192	24.216605715507388
5	15.023314006621568	17.091206923288862	46.90470992618109	20.980769143908475
6	19.93336274245588	6.1104829469747495	55.94798499833633	18.00816931223304
7	81.70733506676522	1.8605435468305145	11.677148057868722	4.754973328535531
8	84.37360827349882	2.0218520821508204	9.159024560632226	4.445515083718137
9	79.94868961280832	4.093087400800671	10.873465995576586	5.084756990814417
10	37.2424882526615	27.350815199798856	19.829808504455784	15.576888043083862
11	25.485574223155016	25.265608038627324	28.383376518572163	20.865441219645493
12	21.899146784142562	21.75729042612614	34.39936885814816	21.94419393158314
13	21.692821213111248	23.010239493641155	35.31015834252996	19.986780950717637
14	20.115297812533406	25.778380681518776	33.320706481399235	20.785615024548587
15	18.270593035458067	25.684552532170617	35.261106335056226	20.783748097315087
16	19.905569616060394	24.477162511498918	33.74323427076166	21.87403360167903
17	19.837486995496793	25.195688602559795	31.400120145800997	23.56670425614242
18	20.90627272494591	24.325218724075683	32.72063993447688	22.04786861650153
19	21.629827474845413	26.806184347019617	30.58062942548326	20.983358752651714
20	23.273506345294216	26.86893719144887	29.81196730468403	20.045589158572884
21	22.620623824701553	27.263912748252388	30.262077438334945	19.85338598871111
22	21.35361257947615	24.439884190288062	30.413328655978006	23.793174574257783
23	19.997049050501886	26.40587901408175	29.446802360157363	24.150269575259
24	20.77245619872537	25.714092138881316	30.9006869991102	22.612764663283112
25	20.73063100634906	27.237564984876382	28.53068309964122	23.501120909133338
26	19.55329249179079	28.526226563664476	29.29811063952797	22.622370305016766
27	20.13173881688003	26.885107190229345	30.536244736093277	22.446909256797344
28	19.037267782105502	27.071559019742757	31.075877041763462	22.81529615638828
29	20.213130821914717	25.59831253867475	30.760426562761122	23.42813007664941
30	21.164239912194198	27.280082747032864	30.049036951608944	21.50664038916399
31	21.799747964823478	28.861641119373438	27.4873718934105	21.851239022392587
32	20.88055730789093	29.47381258158397	26.98881198687731	22.656818123647795
33	21.461231900968546	28.14820391061032	27.08038175650752	23.310182431913613
34	20.611358445512074	28.1981893817008	27.314349895286462	23.876102277500667
35	20.14854116198153	27.206339121309618	27.804779634835054	24.840340081873794
36	20.36384002842547	29.001630550156353	27.41429072573785	23.220238695680322
37	20.678176373433626	28.83107771385727	28.498072096514115	21.99267381619499
38	20.878419375091276	28.976427032504105	26.542285149045536	23.602868443359085
39	21.977196387194688	26.980892601999717	26.87932573815141	24.162585272654184
40	22.327787254608225	26.132645179940166	27.925377111773237	23.614190453678376
41	20.18410311460675	26.134933671387685	27.38950877229962	26.29145444170595
42	19.86678570837091	26.66258150868799	27.229495041350933	26.24113774159017
43	20.301839977235534	25.912317654657606	29.048183284075563	24.7376590840313
44	20.600036435192784	26.333219410623116	27.88689432137948	25.179849832804624
45	21.70146327949858	25.841404531514183	27.065596897287385	25.391535291699853
46	21.712755178088297	26.454389010423174	28.11682748839569	23.716028323092836
47	19.259251452514555	27.39866273808968	29.366584712576017	23.975501096819748
48	19.120376155725822	26.121533951727887	29.355082031879288	25.403007860667003
49	20.930994454925	25.11185754742974	29.69218284444431	24.26496515320095
50	21.055536568437187	24.179176835648676	28.624300466882367	26.140986129031774
51	19.743749181337353	24.60622138444699	27.22058196939745	28.429447464818207
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	781.0
1	1447.5
2	2114.0
3	14960.0
4	27806.0
5	19116.5
6	10427.0
7	10603.5
8	10780.0
9	11568.5
10	12357.0
11	13200.0
12	14043.0
13	14158.0
14	14273.0
15	14038.0
16	13803.0
17	13465.0
18	13127.0
19	13020.0
20	12913.0
21	14072.5
22	15232.0
23	15875.0
24	16518.0
25	19759.0
26	27247.0
27	31494.0
28	35360.5
29	39227.0
30	46499.5
31	53772.0
32	60575.5
33	67379.0
34	76691.5
35	86004.0
36	99265.0
37	112526.0
38	125873.5
39	139221.0
40	154490.0
41	169759.0
42	190745.0
43	211731.0
44	235031.5
45	258332.0
46	298967.5
47	339603.0
48	365242.5
49	390882.0
50	362668.5
51	334455.0
52	283060.5
53	231666.0
54	199609.5
55	167553.0
56	146451.5
57	125350.0
58	112946.0
59	100542.0
60	92014.5
61	83487.0
62	72256.5
63	61026.0
64	52974.0
65	44922.0
66	36833.0
67	28744.0
68	23970.0
69	19196.0
70	16072.0
71	12948.0
72	11139.5
73	9331.0
74	7845.5
75	5377.0
76	4394.0
77	3360.5
78	2327.0
79	1672.0
80	1017.0
81	719.5
82	422.0
83	263.0
84	104.0
85	59.5
86	15.0
87	8.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3320965.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.66011989864341
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.03899041217164	19.786657428941325
2	13.02276123736871	7.464677969487862
3	5.35165054351291	4.601368386981605
4	2.7840986720244256	3.191704069994958
5	1.5888388970448488	2.276815664446686
6	1.0617290776611654	1.8257569599387051
7	0.7654486028332156	1.5356494113414327
8	0.552023429632334	1.2656846144098428
9	0.4481200823117227	1.1558857759239534
>10	4.36847427767602	29.088892382051736
>50	0.8869624624565592	16.800184580496637
>100	0.12093229275826486	6.070100509469568
>500	0.005601130645056027	1.092592996086105
>1k	0.0038087688386374144	2.296336324294078
>5k	4.480904516044017E-4	1.0692841742717725
>10k+	1.1202261290110043E-4	0.4784087518637947
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14901	0.4486948823610005	No Hit
CTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGCT	9170	0.2761245601805499	TruSeq Adapter, Index 15 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC	8665	0.2609181367463975	No Hit
CCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC	8111	0.2442362385631887	No Hit
GCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC	7359	0.22159221792460929	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCC	4867	0.14655378782974227	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCT	4292	0.1292395433255093	No Hit
TCCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTG	4176	0.12574658269509015	TruSeq Adapter, Index 15 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTG	3930	0.1183390972202357	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTC	3901	0.1174658570626309	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTT	3883	0.11692384593032447	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.022345914515811E-5	0.0	0.0	0.28747668222941225	0.0
2	6.022345914515811E-5	0.0	0.0	1.063997964447081	0.0
3	6.022345914515811E-5	0.0	0.0	1.6484064119916952	0.0
4	6.022345914515811E-5	0.0	0.0	2.353382224744916	0.0
5	6.022345914515811E-5	0.0	0.0	3.64893938960513	0.0
6	6.022345914515811E-5	0.0	0.0	4.9290793489241835	0.0
7	9.033518871773717E-5	0.0	0.0	5.974829605250281	0.0
8	9.033518871773717E-5	0.0	0.0	7.464577314124058	0.0
9	9.033518871773717E-5	0.0	0.0	8.22375424010792	0.0
10	9.033518871773717E-5	0.0	0.0	9.459660068684855	0.0
11	1.2044691829031622E-4	0.0	0.0	11.598134879470274	0.0
12	1.2044691829031622E-4	0.0	0.0	13.34660256883165	0.0
13	1.2044691829031622E-4	0.0	0.0	13.979551124447262	0.0
14	1.2044691829031622E-4	0.0	0.0	14.209032615519886	0.0
15	1.2044691829031622E-4	0.0	0.0	14.495575834132548	0.0
16	1.2044691829031622E-4	0.0	0.0	15.153878466048273	0.0
17	1.2044691829031622E-4	0.0	0.0	16.215678274236556	0.0
18	1.2044691829031622E-4	0.0	0.0	17.352727294626714	0.0
19	1.2044691829031622E-4	0.0	0.0	18.174386059473676	0.0
20	1.5055864786289527E-4	0.0	0.0	18.945186113072555	0.0
21	1.5055864786289527E-4	0.0	0.0	20.059049101691826	0.0
22	1.5055864786289527E-4	0.0	0.0	21.264843200696184	0.0
23	1.5055864786289527E-4	0.0	0.0	22.497647521127142	0.0
24	1.5055864786289527E-4	0.0	0.0	23.409671586421418	0.0
25	1.5055864786289527E-4	1.2044691829031622E-4	0.0	24.199743146946744	0.0
26	1.5055864786289527E-4	1.2044691829031622E-4	0.0	24.90020220026408	0.0
27	1.5055864786289527E-4	1.2044691829031622E-4	0.0	25.58768309813563	0.0
28	1.8067037743547433E-4	1.2044691829031622E-4	0.0	26.33827818119131	0.0
29	1.8067037743547433E-4	1.2044691829031622E-4	0.0	27.122959742123147	0.0
30	1.8067037743547433E-4	1.2044691829031622E-4	0.0	27.940703982125676	0.0
31	1.8067037743547433E-4	1.2044691829031622E-4	0.0	28.726800794347426	0.0
32	1.8067037743547433E-4	1.2044691829031622E-4	0.0	29.461978671861942	0.0
33	2.107821070080534E-4	1.2044691829031622E-4	0.0	30.19727699629475	0.0
34	2.107821070080534E-4	1.2044691829031622E-4	0.0	30.903186272664723	0.0
35	2.107821070080534E-4	1.2044691829031622E-4	0.0	31.670884818117624	0.0
36	2.107821070080534E-4	1.2044691829031622E-4	0.0	32.41088057236376	0.0
37	2.4089383658063243E-4	1.2044691829031622E-4	0.0	33.129707780720366	0.0
38	2.4089383658063243E-4	1.2044691829031622E-4	0.0	33.870486439935384	0.0
39	2.4089383658063243E-4	1.2044691829031622E-4	0.0	34.574950353285864	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGCG	30	2.1667456E-6	45.000004	1
TCTACGG	595	0.0	42.73109	2
CGTTTTT	10630	0.0	42.69285	1
TTTACGG	1565	0.0	42.26837	2
CGTTATT	1430	0.0	42.01049	1
CTACGGG	1075	0.0	41.23256	3
TTATGGG	7300	0.0	40.869865	3
CGTATGG	535	0.0	40.79439	2
TTACGGG	2430	0.0	40.74074	3
TACGGGC	765	0.0	40.588238	4
TATGGGA	4675	0.0	40.379677	4
TACGGGT	485	0.0	40.360825	4
TATGGGC	2340	0.0	40.192307	4
TACGGGA	1465	0.0	39.93174	4
TACGGGG	1800	0.0	39.875	4
GTACGGG	765	0.0	39.705883	3
TTTTACG	1100	0.0	39.68182	1
ATAATCG	40	3.4608456E-7	39.375004	33
ATGGGCG	1015	0.0	39.236458	5
GCGTATG	155	0.0	39.193546	1
>>END_MODULE