##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935258.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1177371
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.855217259470464	33.0	31.0	34.0	30.0	34.0
2	32.015178732956734	33.0	31.0	34.0	30.0	34.0
3	31.94010638957474	33.0	31.0	34.0	30.0	34.0
4	35.69507742249469	37.0	35.0	37.0	33.0	37.0
5	33.09853733445108	37.0	35.0	37.0	28.0	37.0
6	34.191186975048645	37.0	35.0	37.0	28.0	37.0
7	35.52830840915905	37.0	35.0	37.0	32.0	37.0
8	35.53526203719983	37.0	35.0	37.0	33.0	37.0
9	37.65608886238917	39.0	37.0	39.0	35.0	39.0
10	37.0891350305044	39.0	37.0	39.0	33.0	39.0
11	36.88460731579086	39.0	37.0	39.0	32.0	39.0
12	37.26582190320638	39.0	37.0	39.0	34.0	39.0
13	37.300556918762226	39.0	37.0	39.0	34.0	39.0
14	38.4394171420903	40.0	38.0	41.0	34.0	41.0
15	38.54271932976097	40.0	38.0	41.0	34.0	41.0
16	38.498213392380144	40.0	38.0	41.0	34.0	41.0
17	38.42412544559022	40.0	38.0	41.0	34.0	41.0
18	38.07978029015493	39.0	38.0	40.0	34.0	41.0
19	37.571337326976796	38.0	37.0	40.0	34.0	41.0
20	37.06845505792142	38.0	35.0	40.0	33.0	41.0
21	36.97756696912018	38.0	35.0	40.0	33.0	41.0
22	36.92804731898442	38.0	35.0	40.0	33.0	41.0
23	36.675579745042135	38.0	35.0	40.0	33.0	41.0
24	36.34941492528693	37.0	35.0	40.0	32.0	41.0
25	36.15200731120437	37.0	35.0	40.0	31.0	41.0
26	36.42096161702641	37.0	35.0	40.0	32.0	41.0
27	36.33243811848602	38.0	35.0	40.0	32.0	41.0
28	36.3315463010385	38.0	35.0	40.0	32.0	41.0
29	36.32971255449642	38.0	35.0	40.0	32.0	41.0
30	35.92672148371244	38.0	35.0	40.0	31.0	41.0
31	35.500665465685834	38.0	35.0	40.0	29.0	41.0
32	34.7736890071184	38.0	35.0	40.0	22.0	41.0
33	33.91685203729326	38.0	33.0	40.0	17.0	41.0
34	33.13570828566357	38.0	33.0	40.0	12.0	41.0
35	32.60532916132638	38.0	33.0	40.0	10.0	41.0
36	32.28405574793332	38.0	31.0	40.0	8.0	41.0
37	32.150079286817835	37.0	31.0	40.0	8.0	41.0
38	32.0404689770684	37.0	31.0	40.0	8.0	41.0
39	31.888168640131276	37.0	30.0	40.0	8.0	41.0
40	31.746221879084842	37.0	30.0	40.0	8.0	41.0
41	31.60972114991791	37.0	29.0	40.0	7.0	41.0
42	31.595433385058744	37.0	29.0	40.0	7.0	41.0
43	31.46948582902076	37.0	29.0	40.0	7.0	41.0
44	31.346756459943382	37.0	27.0	40.0	7.0	41.0
45	31.329875629686818	37.0	27.0	40.0	7.0	41.0
46	31.19358893670729	37.0	26.0	40.0	7.0	41.0
47	31.087026094578516	36.0	25.0	40.0	7.0	41.0
48	30.98710601840881	36.0	25.0	40.0	7.0	41.0
49	30.914600410575765	36.0	25.0	40.0	7.0	41.0
50	30.797987210488454	36.0	24.0	40.0	7.0	41.0
51	30.01306979703084	35.0	23.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	12.0
10	30.0
11	26.0
12	22.0
13	52.0
14	67.0
15	122.0
16	246.0
17	534.0
18	980.0
19	1925.0
20	3321.0
21	5208.0
22	8323.0
23	12981.0
24	21641.0
25	36725.0
26	51059.0
27	50855.0
28	41760.0
29	32959.0
30	29286.0
31	29069.0
32	32201.0
33	40243.0
34	56828.0
35	83635.0
36	110515.0
37	132681.0
38	201521.0
39	192491.0
40	48.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.11953836131517	18.086907185585513	19.681221976760085	33.11233247633923
2	48.78920917875504	19.51440964657699	19.63815993429429	12.05822124037368
3	21.105921582916515	19.679778081845058	47.86256838328785	11.351731951950574
4	18.2319761570482	20.251390598205663	48.5389906834804	12.977642561265734
5	16.0590841799229	28.73512257393804	43.38700375667483	11.818789489464239
6	17.415920725073065	26.852113734753107	45.5633780685952	10.168587471578627
7	56.65928581560104	5.564855937508228	34.07583505963711	3.7000231872536355
8	57.22469807732652	5.1525814717705805	33.355076692053736	4.2676437588491645
9	52.9272421352318	7.020811621825236	34.81281601126578	5.239130231677186
10	24.622230375981744	24.973861255288266	40.52053261036666	9.883375758363337
11	15.471928559477005	21.38170551168663	48.360966933957094	14.78539899487927
12	14.545117894019812	19.248732982212065	51.36647666708285	14.839672456685276
13	15.54930433992344	20.69695958198393	51.395269630388384	12.35846644770425
14	13.399599616433564	23.153194702434494	48.921622835962495	14.525582845169449
15	11.978127540087193	23.349989085853142	50.238285128476925	14.433598245582743
16	13.477400071855005	23.993711412970082	47.623306502368415	14.905582012806498
17	13.06257755626731	23.860193600827607	46.52518195199305	16.552046890912038
18	13.731950251874729	22.972962643041146	49.188828330237456	14.106258774846673
19	12.580996134608377	25.06304300003992	47.567589145647375	14.788371719704324
20	13.221915606890267	26.117935637959487	47.27031666314186	13.38983209200838
21	13.041598612501923	25.208451711482617	48.188463959108894	13.561485716906565
22	12.428367948590546	23.4825726130506	46.63177537072002	17.45728406763883
23	11.875356196135288	24.501707618074505	46.80257964566819	16.820356540122017
24	12.979935806130777	24.054185129411206	46.65988885406554	16.305990210392476
25	11.787873151283664	25.86423480788978	45.14999944792253	17.197892592904022
26	11.908056169210894	25.831534834814175	46.334163148234495	15.92624584774043
27	13.228455601505388	23.98165064367986	47.38506384138899	15.404829913425761
28	10.994835102953953	24.35697838659182	46.86662063189937	17.781565878554847
29	11.952477171596719	22.558649737423465	46.52119000722797	18.96768308375185
30	13.642853442118074	24.126464810157547	46.35539689698489	15.875284850739488
31	12.1455344152353	26.272262523877348	44.113113028943296	17.469090031944052
32	12.375453446704565	25.68417261848644	42.02592046177458	19.914453473034413
33	14.154926526982575	25.009024343218915	42.332026183760256	18.504022946038248
34	12.880476926983933	26.289504327862673	40.13051111331942	20.699507631833978
35	13.038456017686864	25.85854416322468	38.11050212719695	22.99249769189151
36	15.606890266534506	26.511609339791793	39.3904725018707	18.491027891803007
37	14.378900108801728	27.566417042716356	39.52390537901817	18.530777469463747
38	14.778179520304136	27.15686049681876	37.17868029703467	20.88627968584244
39	17.331410405046498	26.39974995137472	37.04677624979722	19.222063393781568
40	16.832672114397244	27.186927485049317	36.53691147480276	19.443488925750678
41	16.69371846257467	27.049757468121772	34.14437760060338	22.112146468700182
42	17.97734104203348	25.50079796427804	36.11784220946498	20.404018784223492
43	18.210572538307808	24.38865913972741	37.416498283039076	19.984270038925708
44	16.34667407299823	25.232912990043072	36.0970331356896	22.3233798012691
45	16.77483138280117	24.131900649837647	36.070873157229116	23.022394810132067
46	17.22609101124454	25.676443533941296	36.59381792145381	20.503647533360343
47	14.845532971340383	26.14307639647995	38.20758282648375	20.80380780569591
48	15.708302650566388	25.031277311909328	37.34056639750767	21.919853640016612
49	17.65051118126742	23.402309042774114	37.928911107883586	21.01826866807489
50	16.15692929416471	23.505080386726018	36.883870929384194	23.454119389725072
51	15.889384059909748	23.587382396882546	34.500085359669974	26.02314818353773
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5812.0
1	6249.0
2	6686.0
3	48704.0
4	90722.0
5	59476.5
6	28231.0
7	28103.0
8	27975.0
9	29177.5
10	30380.0
11	31195.0
12	32010.0
13	31744.0
14	31478.0
15	30129.5
16	28781.0
17	26521.0
18	24261.0
19	22490.0
20	20719.0
21	19330.5
22	17942.0
23	17048.0
24	16154.0
25	16220.5
26	16297.5
27	16308.0
28	16373.5
29	16439.0
30	17996.0
31	19553.0
32	21032.0
33	22511.0
34	24578.5
35	26646.0
36	29229.5
37	31813.0
38	33086.5
39	34360.0
40	37773.5
41	41187.0
42	48439.5
43	55692.0
44	61701.5
45	67711.0
46	82264.5
47	96818.0
48	103357.5
49	109897.0
50	103108.5
51	96320.0
52	78345.0
53	60370.0
54	49226.5
55	38083.0
56	30982.0
57	23881.0
58	19635.5
59	15390.0
60	12861.0
61	10332.0
62	8314.0
63	6296.0
64	5074.5
65	3853.0
66	3294.5
67	2736.0
68	2058.5
69	1381.0
70	1067.0
71	753.0
72	679.0
73	605.0
74	485.0
75	300.0
76	235.0
77	225.5
78	216.0
79	161.0
80	106.0
81	64.0
82	22.0
83	19.0
84	16.0
85	12.5
86	9.0
87	16.0
88	23.0
89	13.0
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1177371.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.989630411897906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.83181995904927	31.902975344068086
2	9.364995619994597	7.677354185452424
3	3.684630642823419	4.530949447610572
4	2.0678320325380017	3.390386830704654
5	1.2981071593730906	2.6604466348870823
6	0.9323692582262396	2.2930482781265376
7	0.7156560158227369	2.0534132913437744
8	0.5628473102440511	1.8456722580187597
9	0.42501328447582454	1.5679023705729787
>10	2.8488427973587513	21.719634264877673
>50	0.18720526774161617	5.148669318090847
>100	0.07278333993446234	5.049493605564904
>500	0.0036284948947557464	1.1057687476383926
>1k	0.0038419357709178485	3.1440787628673874
>5k	2.1344087616210268E-4	0.5768125131086258
>10k+	2.1344087616210268E-4	5.333394147067335
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	60961	5.177722230291047	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCC	6593	0.5599764220453876	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4287	0.3641163235717544	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC	3757	0.3191007762209193	No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC	3136	0.2663561443249409	No Hit
CTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGCT	2907	0.2469060304695801	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCG	2686	0.22813539657423193	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTC	2145	0.18218556427837954	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGT	1961	0.16655752519808964	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTC	1756	0.14914585122276666	No Hit
TCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC	1718	0.14591832141270678	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCG	1509	0.1281669074573775	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGC	1473	0.12510924763732076	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCT	1459	0.12392015770729872	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTG	1379	0.11712535810717267	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1336	0.11347315332210493	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGC	1192	0.10124251404187805	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.493499500157554E-5	0.0	0.0	0.26092030464484006	0.0
2	8.493499500157554E-5	0.0	0.0	1.1453484075962461	0.0
3	8.493499500157554E-5	0.0	0.0	1.6411139734204427	0.0
4	8.493499500157554E-5	0.0	0.0	2.328068212993186	0.0
5	8.493499500157554E-5	0.0	0.0	4.085458194570785	0.0
6	8.493499500157554E-5	0.0	0.0	5.16710535591585	0.0
7	8.493499500157554E-5	0.0	0.0	6.28518963011659	0.0
8	8.493499500157554E-5	0.0	0.0	7.9085521895817035	0.0
9	8.493499500157554E-5	0.0	0.0	8.634576526855172	0.0
10	8.493499500157554E-5	0.0	0.0	10.39510910324783	0.0
11	8.493499500157554E-5	0.0	0.0	13.060624051382275	0.0
12	8.493499500157554E-5	0.0	0.0	14.812323388294768	0.0
13	8.493499500157554E-5	0.0	0.0	15.498937887887505	0.0
14	8.493499500157554E-5	0.0	0.0	15.784998951052811	0.0
15	8.493499500157554E-5	0.0	0.0	16.241694419176284	0.0
16	8.493499500157554E-5	0.0	0.0	17.172836769378556	0.0
17	8.493499500157554E-5	0.0	0.0	18.470983232982636	0.0
18	8.493499500157554E-5	0.0	0.0	19.755540097386465	0.0
19	8.493499500157554E-5	0.0	0.0	20.648037024863022	0.0
20	8.493499500157554E-5	0.0	0.0	21.39189771108682	0.0
21	8.493499500157554E-5	0.0	0.0	22.29976787265866	0.0
22	8.493499500157554E-5	0.0	0.0	23.23762008746606	0.0
23	8.493499500157554E-5	0.0	0.0	24.15364400855805	0.0
24	8.493499500157554E-5	0.0	0.0	24.775962716934593	0.0
25	8.493499500157554E-5	0.0	0.0	25.265018418153666	0.0
26	8.493499500157554E-5	0.0	0.0	25.689353653181538	0.0
27	8.493499500157554E-5	0.0	0.0	26.083452029988848	0.0
28	8.493499500157554E-5	0.0	0.0	26.477975081771167	0.0
29	8.493499500157554E-5	0.0	0.0	26.873942028468512	0.0
30	8.493499500157554E-5	0.0	0.0	27.293690773766297	0.0
31	8.493499500157554E-5	0.0	0.0	27.664432026948177	0.0
32	8.493499500157554E-5	0.0	0.0	27.98650552799415	0.0
33	8.493499500157554E-5	0.0	0.0	28.289043980189764	0.0
34	8.493499500157554E-5	0.0	0.0	28.586911007660287	0.0
35	8.493499500157554E-5	0.0	0.0	28.89284685965596	0.0
36	8.493499500157554E-5	0.0	0.0	29.186891812351416	0.0
37	1.6986999000315108E-4	0.0	0.0	29.46802664580663	0.0
38	1.6986999000315108E-4	0.0	0.0	29.759098873677033	0.0
39	1.6986999000315108E-4	0.0	0.0	30.04583941680235	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTACG	35	1.2119563E-7	45.0	1
CGTTGTT	105	0.0	45.0	1
CGTTGCC	20	7.033357E-4	45.0	28
CGTTGAT	20	7.033357E-4	45.0	25
GCGTTAG	70	0.0	45.0	1
CCGTCGA	20	7.033357E-4	45.0	41
TCGCTCG	20	7.033357E-4	45.0	30
TTGAACG	50	2.1827873E-11	45.0	1
GTCCGAC	20	7.033357E-4	45.0	22
AACTGCG	25	3.8908638E-5	45.0	1
ACGCTAG	20	7.033357E-4	45.0	1
CCGACTA	25	3.8908638E-5	45.0	14
CCGACCA	20	7.033357E-4	45.0	15
CATAACG	20	7.033357E-4	45.0	1
GTATACG	45	3.8562575E-10	45.0	1
CGTCGAA	20	7.033357E-4	45.0	42
ACGTACG	20	7.033357E-4	45.0	1
GCGGACC	25	3.8908638E-5	45.0	5
GGACCGC	20	7.033357E-4	45.0	18
TAAATCG	20	7.033357E-4	45.0	39
>>END_MODULE