FastQCFastQC Report
Sat 14 Jan 2017
SRR2935257.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935257.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224427
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63582.832992465255963No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42021.8723237400134565No Hit
GCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC23591.0511213000218333No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCG17440.7770901005672224No Hit
GAATCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTC16170.7205015439318798No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15880.7075797475348332No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCC15720.7004504805571522No Hit
CCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC15280.6808449963685297No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGC13910.6198006478721366No Hit
CTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGCT12600.5614297744923739No Hit
TCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC7270.3239360682983776No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5660.2521978193354632No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5620.25041550259104295No Hit
ACTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC5410.24105833968283674No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5340.2379392853801013No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACGTGAAT4970.22145285549421417No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCG4720.2103133758415877No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCT4510.20095621293338145No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4260.189816733280755No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGC4080.18179630793086393No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3980.17734051606981335No Hit
AAAAGGGGATGACGACTTTATTTATTGTATTAAGATTTTATAGTATTTATA3900.17377588258097287No Hit
GAACTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCT3290.1465955522285643No Hit
ACCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTG2780.1238710137372063No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTC2700.12030638024836585No Hit
CGCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTG2590.11540500920121019No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACGTGAATCGTA2530.11273153408457985No Hit
GCCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTG2490.11094921734015961No Hit
AGCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTG2480.11050363815405455No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTC2340.10426552954858372No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGT2340.10426552954858372No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG207.0211437E-445.04
ATTTAGG207.0211437E-445.02
GGCAGTA207.0211437E-445.08
TAATACG253.8807426E-545.04
TACTTTC253.8807426E-545.011
CTTCCAT253.8807426E-545.027
TAGAAAG253.8807426E-545.01
ACTCTCA207.0211437E-445.027
GATCCGG207.0211437E-445.05
AAGACAC207.0211437E-445.021
AATGGGT207.0211437E-445.04
TCGATGG207.0211437E-445.02
CGTTCAA207.0211437E-445.011
AGAACGG253.8807426E-545.02
ATCCGGC207.0211437E-445.06
GAGACAT253.8807426E-545.033
TCTACTC207.0211437E-445.028
CAGATTA253.8807426E-545.020
ATATAAG207.0211437E-445.01
TCTAAGT207.0211437E-445.038
Produced by FastQC (version 0.11.5)