##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935257.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	224427
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.59611365833879	31.0	31.0	34.0	30.0	34.0
2	31.695963498153073	31.0	31.0	34.0	30.0	34.0
3	31.707766890792996	31.0	31.0	34.0	30.0	34.0
4	35.54466262971924	37.0	35.0	37.0	33.0	37.0
5	32.95347262138691	37.0	35.0	37.0	28.0	37.0
6	33.987590619666975	37.0	35.0	37.0	26.0	37.0
7	35.22967824726971	37.0	35.0	37.0	32.0	37.0
8	35.067670110993774	37.0	35.0	37.0	32.0	37.0
9	37.20818796312387	39.0	37.0	39.0	34.0	39.0
10	36.79430728031832	39.0	37.0	39.0	32.0	39.0
11	36.643416344735705	39.0	35.0	39.0	32.0	39.0
12	36.94321093273091	39.0	37.0	39.0	33.0	39.0
13	36.81246908794397	39.0	37.0	39.0	33.0	39.0
14	37.742620094730135	40.0	37.0	41.0	33.0	41.0
15	37.922152860395585	40.0	37.0	41.0	33.0	41.0
16	37.94306834739135	40.0	37.0	41.0	33.0	41.0
17	37.825373061173565	39.0	37.0	41.0	33.0	41.0
18	37.662304446434696	39.0	37.0	40.0	33.0	41.0
19	37.35394582648255	39.0	36.0	40.0	33.0	41.0
20	37.051776301425406	39.0	35.0	40.0	32.0	41.0
21	36.99120872265815	38.0	35.0	40.0	32.0	41.0
22	36.93185311927709	38.0	35.0	40.0	32.0	41.0
23	36.59751277698316	38.0	35.0	40.0	31.0	41.0
24	36.27707450529571	38.0	35.0	40.0	31.0	41.0
25	36.06091513053242	38.0	35.0	40.0	31.0	41.0
26	36.353812152726725	38.0	35.0	40.0	31.0	41.0
27	36.2080810241192	38.0	35.0	40.0	31.0	41.0
28	36.321627968114356	38.0	35.0	40.0	31.0	41.0
29	36.393695945674985	38.0	35.0	40.0	31.0	41.0
30	35.95915821180161	38.0	35.0	40.0	30.0	41.0
31	35.87137911213891	38.0	35.0	40.0	30.0	41.0
32	35.49423197743587	38.0	35.0	40.0	29.0	41.0
33	35.238006122258014	38.0	35.0	40.0	27.0	41.0
34	35.17953722145731	38.0	35.0	40.0	25.0	41.0
35	34.85450057256926	38.0	35.0	40.0	24.0	41.0
36	34.61096926840354	38.0	34.0	40.0	23.0	41.0
37	34.56191545580523	38.0	34.0	40.0	22.0	41.0
38	34.437077535234174	38.0	34.0	40.0	21.0	41.0
39	34.3153987710926	38.0	34.0	40.0	21.0	41.0
40	34.14007672873585	37.0	34.0	40.0	20.0	41.0
41	33.926728958636886	37.0	33.0	40.0	18.0	41.0
42	33.99350345546659	37.0	34.0	40.0	18.0	41.0
43	33.936634183944	37.0	34.0	40.0	18.0	41.0
44	33.86940074055261	37.0	33.0	40.0	17.0	41.0
45	33.901076964892816	37.0	33.0	40.0	18.0	41.0
46	33.71341237908095	37.0	33.0	40.0	17.0	41.0
47	33.546814777188125	37.0	33.0	40.0	15.0	41.0
48	33.475428535782235	37.0	33.0	40.0	15.0	41.0
49	33.459971393816254	37.0	33.0	40.0	15.0	41.0
50	33.38118408212915	37.0	33.0	40.0	15.0	41.0
51	32.518137300770405	35.0	32.0	39.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	3.0
10	3.0
11	11.0
12	6.0
13	16.0
14	19.0
15	27.0
16	39.0
17	73.0
18	149.0
19	307.0
20	575.0
21	940.0
22	1380.0
23	1968.0
24	2715.0
25	3446.0
26	4187.0
27	4654.0
28	4598.0
29	4644.0
30	5037.0
31	6045.0
32	7598.0
33	10179.0
34	15119.0
35	22932.0
36	26198.0
37	30088.0
38	42205.0
39	29258.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.104595258146304	23.697237854625335	19.607712084553107	18.590454802675257
2	34.37955326230801	29.686267695063428	21.418545896884066	14.515633145744497
3	23.724863764163846	29.603835545633995	34.66472394141525	12.00657674878691
4	22.52982038703008	26.790002985380546	36.3784214911753	14.301755136414068
5	19.035142830408105	34.13314797239192	31.907925516983248	14.92378368021673
6	18.666203264313115	35.896750390995734	33.069996034345245	12.367050310345903
7	56.465131200791355	12.302886907546775	25.520993463353342	5.710988428308537
8	53.42405325562433	14.732184630191556	23.341220084927393	8.502542029256729
9	48.800278041412135	14.128870412205305	25.985732554460917	11.08511899192165
10	26.75168317537551	28.40790101012802	31.688700557419562	13.151715257076912
11	19.328333934865235	26.986948985638982	35.05326899169886	18.631448087796922
12	17.776381629661316	24.00513307222393	40.56731141974896	17.651173878365796
13	17.25995535296555	28.061685982524388	41.240581569953704	13.437777094556358
14	13.24840594046171	31.94669090617439	36.67473164993517	18.130171503428734
15	10.326297637984734	29.685822115877325	43.13161963578357	16.856260610354372
16	11.084673412735544	30.19021775454825	36.89752124298769	21.82758758972851
17	10.74959786478454	31.33535626283825	35.72252892922866	22.19251694314855
18	11.46653477522758	29.522294554576767	40.45547104403659	18.555699626159065
19	11.671501200835907	31.144648371185284	37.02718478614427	20.15666564183454
20	12.982395166356989	33.64924897628182	37.94641464707901	15.421941210282187
21	13.378960641990492	33.44873834253455	37.39211413956431	15.780186875910651
22	10.779897249439683	31.53185668391058	35.16555494659733	22.5226911200524
23	11.47009940871642	32.100415725380635	35.03143561157972	21.398049254323233
24	15.15281138187473	29.741519514140457	34.237413501940495	20.868255602044318
25	10.815989163514192	33.70984774559211	32.474256662522784	22.999906428370917
26	10.97951672481475	32.28488550842813	34.90266322679535	21.83293453996177
27	14.453697638875893	33.22773106622643	33.565034510107964	18.75353678478971
28	9.780017555819933	30.946365633368533	34.27840678706216	24.99521002374937
29	14.516524304116707	28.22966933568599	33.128812486911116	24.12499387328619
30	15.328369581200125	34.084134261920354	31.652163064158945	18.935333092720573
31	16.067139871762308	33.78782410316049	26.45047164556849	23.694564379508705
32	17.914065598167777	35.89051228239026	25.176115173308023	21.01930694613393
33	15.941486541280684	33.872484148520456	28.229223756499888	21.956805553698974
34	17.769252362683634	29.179644160461976	28.673466205046626	24.37763727180776
35	15.789989618004963	33.868919515031614	26.15371590762252	24.1873749593409
36	16.72035895859233	35.37854179755555	26.23703921542417	21.664060028427954
37	17.567850570564147	33.37165314333837	29.479964531896783	19.5805317542007
38	17.587901633938877	33.48260236067853	27.039527329599377	21.889968675783216
39	22.42199022399266	28.761245304709327	30.99270586872346	17.824058602574556
40	21.307596679543906	29.135977400223677	31.267628226550283	18.28879769368213
41	16.848240185004478	35.63073961689101	25.04867952608198	22.47234067202253
42	16.863835456518157	33.68756878628685	28.332598127676263	21.11599762951873
43	20.487730977110598	29.632352613544715	28.78263310564237	21.097283303702316
44	18.7089788661792	26.37071297125569	29.75711478565413	25.163193376910975
45	20.207461669050517	24.4868041724035	29.031711870675096	26.274022287870892
46	22.49729310644441	27.457926185352026	31.15667900921012	18.888101698993438
47	15.421050051909976	29.283909690010564	35.427109928841006	19.86793032923846
48	16.877202832101307	28.107580638693207	31.851782539534014	23.163433989671475
49	19.328779514051337	24.90163839466731	35.844172047035336	19.925410044246014
50	19.029795880174845	23.175019048510208	32.98934620166023	24.80583886965472
51	16.93468254710886	23.298890062247413	29.51739318352961	30.249034207114118
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5485.0
1	5309.0
2	5133.0
3	7586.5
4	10040.0
5	6518.0
6	2996.0
7	3145.0
8	3294.0
9	3605.0
10	3916.0
11	4223.5
12	4531.0
13	4356.0
14	4181.0
15	3991.0
16	3801.0
17	3611.5
18	3422.0
19	3066.0
20	2710.0
21	2608.0
22	2506.0
23	2408.0
24	2310.0
25	2015.5
26	2080.5
27	2440.0
28	2499.0
29	2558.0
30	2677.0
31	2796.0
32	2689.5
33	2583.0
34	2788.0
35	2993.0
36	3138.0
37	3283.0
38	3342.0
39	3401.0
40	4385.0
41	5369.0
42	7282.5
43	9196.0
44	11404.5
45	13613.0
46	19503.0
47	25393.0
48	27038.0
49	28683.0
50	27454.0
51	26225.0
52	20307.0
53	14389.0
54	11086.0
55	7783.0
56	6452.5
57	5122.0
58	4287.5
59	3453.0
60	3087.5
61	2722.0
62	2407.0
63	2092.0
64	1661.0
65	1230.0
66	1109.5
67	989.0
68	827.5
69	666.0
70	539.5
71	413.0
72	449.0
73	485.0
74	362.0
75	193.0
76	147.0
77	93.0
78	39.0
79	47.5
80	56.0
81	34.5
82	13.0
83	9.5
84	6.0
85	3.5
86	1.0
87	1.0
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	224427.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.9937307008515
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.06112977032858	31.180294706073692
2	9.619460658036326	7.886751594059538
3	3.9543048445125595	4.863051237150611
4	2.2771491614221584	3.7339535795603918
5	1.5249834240932163	3.1257379905269866
6	1.1260747165791678	2.769720220829045
7	0.8336865903631483	2.3923146501980597
8	0.6945576678514364	2.27780079936906
9	0.5608634688753383	2.0692697402718925
>10	3.1869218812838986	20.571499864098346
>50	0.08804252127694265	2.5246516684712623
>100	0.05652112477038293	4.775272137487914
>500	0.005434723535613743	1.305547015287822
>1k	0.009782502364104738	7.691142331359417
>5k	0.0010869447071227487	2.832992465255963
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6358	2.832992465255963	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4202	1.8723237400134565	No Hit
GCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC	2359	1.0511213000218333	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCG	1744	0.7770901005672224	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTC	1617	0.7205015439318798	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1588	0.7075797475348332	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCC	1572	0.7004504805571522	No Hit
CCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC	1528	0.6808449963685297	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGC	1391	0.6198006478721366	No Hit
CTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGCT	1260	0.5614297744923739	No Hit
TCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC	727	0.3239360682983776	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	566	0.2521978193354632	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	562	0.25041550259104295	No Hit
ACTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC	541	0.24105833968283674	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	534	0.2379392853801013	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACGTGAAT	497	0.22145285549421417	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCG	472	0.2103133758415877	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCT	451	0.20095621293338145	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	426	0.189816733280755	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGC	408	0.18179630793086393	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	398	0.17734051606981335	No Hit
AAAAGGGGATGACGACTTTATTTATTGTATTAAGATTTTATAGTATTTATA	390	0.17377588258097287	No Hit
GAACTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCT	329	0.1465955522285643	No Hit
ACCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTG	278	0.1238710137372063	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTC	270	0.12030638024836585	No Hit
CGCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTG	259	0.11540500920121019	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACGTGAATCGTA	253	0.11273153408457985	No Hit
GCCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTG	249	0.11094921734015961	No Hit
AGCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTG	248	0.11050363815405455	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTC	234	0.10426552954858372	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGT	234	0.10426552954858372	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6028686388001444	0.0
2	0.0	0.0	0.0	3.0646936420305932	0.0
3	0.0	0.0	0.0	3.9513962223796604	0.0
4	0.0	0.0	0.0	5.231545224059493	0.0
5	0.0	0.0	0.0	8.861678853257407	0.0
6	0.0	0.0	0.0	10.492498674401922	0.0
7	0.0	0.0	0.0	12.435223925819978	0.0
8	0.0	0.0	0.0	14.991511716504698	0.0
9	0.0	0.0	0.0	15.860391129409564	0.0
10	0.0	0.0	0.0	19.043608834944102	0.0
11	0.0	0.0	0.0	23.287750582594786	0.0
12	0.0	0.0	0.0	26.70757083595111	0.0
13	0.0	0.0	0.0	27.765821402950625	0.0
14	0.0	0.0	0.0	28.174863095795068	0.0
15	0.0	0.0	0.0	29.094984115102015	0.0
16	0.0	0.0	0.0	30.76902511729872	0.0
17	0.0	0.0	0.0	32.81423358152094	0.0
18	0.0	0.0	0.0	34.79750653887456	0.0
19	0.0	0.0	0.0	36.308019979770705	0.0
20	0.0	0.0	0.0	37.47632860573817	0.0
21	4.4557918610505865E-4	0.0	0.0	38.84069207359186	0.0
22	4.4557918610505865E-4	0.0	0.0	40.15604183097399	0.0
23	4.4557918610505865E-4	0.0	0.0	41.28781296368084	0.0
24	4.4557918610505865E-4	0.0	0.0	42.170950910541066	0.0
25	4.4557918610505865E-4	0.0	0.0	42.8522414860957	0.0
26	4.4557918610505865E-4	0.0	0.0	43.42748421535733	0.0
27	4.4557918610505865E-4	0.0	0.0	43.904253944489746	0.0
28	4.4557918610505865E-4	0.0	0.0	44.376567881761105	0.0
29	4.4557918610505865E-4	0.0	0.0	44.821255909493956	0.0
30	4.4557918610505865E-4	0.0	0.0	45.29668890106805	0.0
31	4.4557918610505865E-4	0.0	0.0	45.695482272632084	0.0
32	4.4557918610505865E-4	0.0	0.0	46.04704425046897	0.0
33	4.4557918610505865E-4	0.0	0.0	46.37989190248945	0.0
34	4.4557918610505865E-4	0.0	0.0	46.705164708346146	0.0
35	4.4557918610505865E-4	0.0	0.0	47.05628110699693	0.0
36	4.4557918610505865E-4	0.0	0.0	47.35704705761785	0.0
37	4.4557918610505865E-4	0.0	0.0	47.68410218021896	0.0
38	4.4557918610505865E-4	0.0	0.0	47.96303475072072	0.0
39	4.4557918610505865E-4	0.0	0.0	48.20944004063682	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCG	20	7.0211437E-4	45.0	4
ATTTAGG	20	7.0211437E-4	45.0	2
GGCAGTA	20	7.0211437E-4	45.0	8
TAATACG	25	3.8807426E-5	45.0	4
TACTTTC	25	3.8807426E-5	45.0	11
CTTCCAT	25	3.8807426E-5	45.0	27
TAGAAAG	25	3.8807426E-5	45.0	1
ACTCTCA	20	7.0211437E-4	45.0	27
GATCCGG	20	7.0211437E-4	45.0	5
AAGACAC	20	7.0211437E-4	45.0	21
AATGGGT	20	7.0211437E-4	45.0	4
TCGATGG	20	7.0211437E-4	45.0	2
CGTTCAA	20	7.0211437E-4	45.0	11
AGAACGG	25	3.8807426E-5	45.0	2
ATCCGGC	20	7.0211437E-4	45.0	6
GAGACAT	25	3.8807426E-5	45.0	33
TCTACTC	20	7.0211437E-4	45.0	28
CAGATTA	25	3.8807426E-5	45.0	20
ATATAAG	20	7.0211437E-4	45.0	1
TCTAAGT	20	7.0211437E-4	45.0	38
>>END_MODULE