SRR2935255.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935255.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	319283
Sequences flagged as poor quality	0
Sequence length	51
%GC	39

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8702	2.725481782619181	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3086	0.9665406551554577	No Hit
CCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC	2286	0.7159792409868362	No Hit
GCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC	1899	0.5947701568827655	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCC	1868	0.5850609020837313	No Hit
CTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGCT	1220	0.3821061566071479	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1062	0.33262027730884514	No Hit
TCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC	1009	0.31602058362017393	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1008	0.31570738185246316	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	888	0.27812316972716994	No Hit
ACTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC	695	0.21767522855899	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	680	0.21297720204332835	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTC	655	0.2051471578505589	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCG	609	0.19073987653586316	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCG	606	0.18980027123273085	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGC	571	0.17883820936285363	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	556	0.174140182847192	No Hit
TAAAACGGGAATTTTTCCTTTTATATGCTGTCTCTTATACACATCTGACGC	502	0.15722728739081004	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	473	0.1481444361271975	No Hit
ACCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTG	458	0.14344640961153585	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGC	404	0.1265335141551539	No Hit
CGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTG	396	0.12402790001346767	No Hit
GCCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTG	394	0.12340149647804613	No Hit
GGTTGGGAGAACTGTATGATTTTAATAATTGATGAATAACACTAGTATATT	389	0.12183548763949224	No Hit
GGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTG	364	0.11400544344672282	No Hit
AGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTG	355	0.11118662753732582	No Hit
GGCCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCT	323	0.10116417097058095	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCTAGG	40	6.7884685E-9	45.000004	2
CGGGTCT	20	7.025624E-4	45.000004	6
CGGGTAT	20	7.025624E-4	45.000004	6
GCGACGG	20	7.025624E-4	45.000004	2
TTAGCGG	20	7.025624E-4	45.000004	2
ATTACGG	20	7.025624E-4	45.000004	2
ATTCGAC	40	6.7884685E-9	45.000004	9
CGCGAGG	20	7.025624E-4	45.000004	2
AATGCGG	20	7.025624E-4	45.000004	2
CGGGCTA	20	7.025624E-4	45.000004	6
TATTACG	20	7.025624E-4	45.000004	1
CGACTGA	40	6.7884685E-9	45.000004	12
GGATTCG	40	6.7884685E-9	45.000004	7
GATTCGA	40	6.7884685E-9	45.000004	8
TTCGACT	40	6.7884685E-9	45.000004	10
TCGCGGG	20	7.025624E-4	45.000004	3
TGACTAC	40	6.7884685E-9	45.000004	16
AAGTAGT	70	0.0	45.0	4
TCCGCTT	25	3.8844562E-5	45.0	27
CACGCGG	35	1.2082455E-7	45.0	37

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content