##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935255.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	319283
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.599906665873224	31.0	31.0	34.0	30.0	34.0
2	31.705064159382115	31.0	31.0	34.0	30.0	34.0
3	31.704456547952756	31.0	31.0	34.0	30.0	34.0
4	35.533066276626066	37.0	35.0	37.0	33.0	37.0
5	33.0956612159119	37.0	35.0	37.0	28.0	37.0
6	34.075938900599155	37.0	35.0	37.0	28.0	37.0
7	35.27979253514907	37.0	35.0	37.0	32.0	37.0
8	35.18055455504991	37.0	35.0	37.0	32.0	37.0
9	37.208191479032706	39.0	37.0	39.0	34.0	39.0
10	36.80389184516557	39.0	37.0	39.0	32.0	39.0
11	36.67210280534823	39.0	37.0	39.0	32.0	39.0
12	37.01182649874876	39.0	37.0	39.0	33.0	39.0
13	36.93453769853077	39.0	37.0	39.0	33.0	39.0
14	37.88453816833342	40.0	37.0	41.0	33.0	41.0
15	38.028745658240496	40.0	37.0	41.0	33.0	41.0
16	37.99210105141833	40.0	37.0	41.0	33.0	41.0
17	37.927496922792635	39.0	37.0	41.0	33.0	41.0
18	37.77656499093281	39.0	37.0	40.0	33.0	41.0
19	37.536552212300684	39.0	36.0	40.0	33.0	41.0
20	37.3138970756351	39.0	35.0	40.0	33.0	41.0
21	37.23547761703567	39.0	35.0	40.0	33.0	41.0
22	37.17622923863782	39.0	35.0	40.0	33.0	41.0
23	36.89283488316008	39.0	35.0	40.0	32.0	41.0
24	36.61133853039467	38.0	35.0	40.0	31.0	41.0
25	36.404650419846966	38.0	35.0	40.0	31.0	41.0
26	36.70217643908382	38.0	35.0	40.0	32.0	41.0
27	36.614276362975794	38.0	35.0	40.0	32.0	41.0
28	36.66943745830501	38.0	35.0	40.0	32.0	41.0
29	36.73354046410238	38.0	35.0	40.0	32.0	41.0
30	36.47995665287535	38.0	35.0	40.0	31.0	41.0
31	36.30645540163429	38.0	35.0	40.0	31.0	41.0
32	36.10161518151608	38.0	35.0	40.0	30.0	41.0
33	35.91523507358676	38.0	35.0	40.0	30.0	41.0
34	35.781397694208586	39.0	35.0	40.0	29.0	41.0
35	35.540201012894514	39.0	35.0	40.0	27.0	41.0
36	35.32266672513099	38.0	35.0	40.0	25.0	41.0
37	35.27601532183048	38.0	35.0	40.0	24.0	41.0
38	35.18065477961557	38.0	35.0	40.0	24.0	41.0
39	35.0921721482196	38.0	35.0	40.0	23.0	41.0
40	35.018234606916124	38.0	35.0	40.0	23.0	41.0
41	34.80920061512827	38.0	35.0	40.0	22.0	41.0
42	34.79986407043281	38.0	35.0	40.0	22.0	41.0
43	34.674752492303064	38.0	35.0	40.0	21.0	41.0
44	34.59610439641321	38.0	35.0	40.0	21.0	41.0
45	34.599079813206465	38.0	34.0	40.0	20.0	41.0
46	34.479136064243946	38.0	34.0	40.0	20.0	41.0
47	34.332294547470426	38.0	34.0	40.0	20.0	41.0
48	34.283087417745385	38.0	34.0	40.0	20.0	41.0
49	34.25176724097431	38.0	34.0	40.0	20.0	41.0
50	34.181068205948954	38.0	34.0	40.0	20.0	41.0
51	33.2771616403003	36.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	9.0
10	14.0
11	19.0
12	5.0
13	12.0
14	24.0
15	34.0
16	49.0
17	110.0
18	200.0
19	337.0
20	575.0
21	977.0
22	1509.0
23	2118.0
24	3030.0
25	4172.0
26	5271.0
27	5965.0
28	6270.0
29	6377.0
30	6761.0
31	7943.0
32	9697.0
33	12871.0
34	19609.0
35	29090.0
36	34979.0
37	43184.0
38	64618.0
39	53433.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.67863932624036	23.62543574195933	22.67862679816965	19.017298133630668
2	37.7314169561173	24.976901369631328	22.210390155442035	15.081291518809332
3	25.456413275996532	24.66307319838513	35.97936626754321	13.901147258075126
4	23.946154352095164	24.545622535493592	35.64580638493124	15.86241672748001
5	18.17635138732723	32.23879755577341	33.161489963449355	16.423361093450012
6	19.774933209723038	31.888951181240465	35.16316246088893	13.172953148147567
7	60.320154846953955	9.794132478083705	24.052329751349117	5.833382923613221
8	57.386393888807106	10.087289332660994	24.222085109448358	8.30423166908354
9	50.982357344424855	12.417197282661462	27.057187510766312	9.543257862147374
10	26.678213371836268	26.114136988189163	33.41800221120448	13.789647428770088
11	18.254651829254925	25.420708274477498	36.891409815117	19.43323008115058
12	17.65299123348252	21.420182095507748	43.101887667053994	17.824939003955738
13	18.508658462868365	24.598553634236712	42.680318087715285	14.212469815179638
14	14.025801561624013	27.614060253756072	39.84490248462962	18.51523569999029
15	11.494818076753226	26.120401023543376	44.414516275529856	17.970264624173538
16	12.330440393005578	27.872451712117464	39.42458571236176	20.372522182515198
17	12.601986325610822	27.925069609092873	37.15262008938778	22.320323975908522
18	12.901720417310036	27.050923475412098	41.55717654870444	18.490179558573427
19	13.452015923177871	27.91536035429384	39.42176689645237	19.210856826075926
20	13.82316001791514	30.049830401242783	39.28239210982107	16.844617471021007
21	14.89963449353708	28.690221527610305	40.10485995182957	16.30528402702305
22	12.27500368012077	28.00462285809141	37.124118728526106	22.596254733261713
23	12.381179079374723	27.6002793759768	38.53321348145689	21.485328063191588
24	15.473733333750936	26.65315723041941	36.68688906080186	21.186220375027798
25	12.903599627916302	30.187639179035525	34.10955171430988	22.799209478738298
26	12.538093164997823	28.4954100280942	36.830961873948816	22.13553493295916
27	17.377373677897037	27.470927045912248	35.7494761700435	19.40222310614721
28	11.946768227559875	26.340895068011765	35.919231528142745	25.793105176285614
29	14.178644024266873	24.607949687268036	36.66277252468813	24.550633763776965
30	18.75608785935988	25.92778193640125	35.13090267881472	20.185227525424153
31	15.002677875113926	28.74064701221174	32.89683446973375	23.35984064294059
32	14.71766426649712	27.21566760522796	33.230394352345726	24.83627377592919
33	16.307163237629315	26.761525042047335	34.34977747014404	22.58153425017931
34	13.644635010319996	23.94803356270143	36.100888553415	26.30644287356358
35	14.58893833996799	24.510857139277693	32.76215770961811	28.138046811136203
36	17.28591876172549	27.99647961213093	31.888011575937337	22.829590050206246
37	16.13991349367176	26.404788228624763	35.25994180711156	22.19535647059192
38	14.270725343973842	27.614999859059203	33.7208683205808	24.393406476386154
39	16.431817541178205	25.792478772750194	37.92936047331052	19.84634321276109
40	17.42247473244739	23.124939317157505	37.892089462952924	21.560496487442173
41	15.618432550433315	26.94255566378417	30.687195998534218	26.7518157872483
42	17.191958231412258	26.4436252478209	31.17673036146616	25.187686159300682
43	19.12409993642004	24.955916851194708	33.43836032610568	22.48162288627957
44	17.11585020185854	22.81706197949781	33.47876335414037	26.588324464503277
45	18.85819163563359	20.61149513127852	33.85773749307041	26.67257574001748
46	20.241290641844383	21.70770131826624	36.08930008800969	21.96170795187968
47	14.486834563694279	24.54844135140299	38.206230835966835	22.758493248935896
48	15.131090599875346	22.964266810321877	36.29006242111231	25.61458016869047
49	17.638270750400114	21.094765458856248	39.1380060949064	22.128957695837233
50	17.411825872345226	19.772427595581348	37.13100916741574	25.684737364657686
51	15.618119348665605	19.825985097859892	33.24887325664066	31.307022296833843
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4063.0
1	5227.5
2	6392.0
3	10370.5
4	14349.0
5	9195.5
6	4042.0
7	4041.0
8	4040.0
9	4322.5
10	4605.0
11	4752.0
12	4899.0
13	5018.0
14	5137.0
15	4913.5
16	4690.0
17	4290.0
18	3890.0
19	3904.0
20	3918.0
21	4039.5
22	4161.0
23	4012.0
24	3863.0
25	3712.5
26	3579.5
27	3597.0
28	3904.0
29	4211.0
30	4352.5
31	4494.0
32	4518.0
33	4542.0
34	5127.0
35	5712.0
36	5816.0
37	5920.0
38	6453.0
39	6986.0
40	8010.5
41	9035.0
42	11525.5
43	14016.0
44	16369.5
45	18723.0
46	24467.0
47	30211.0
48	34922.5
49	39634.0
50	38436.0
51	37238.0
52	29156.5
53	21075.0
54	16448.5
55	11822.0
56	9734.5
57	7647.0
58	6553.0
59	5459.0
60	5204.5
61	4950.0
62	4358.5
63	3767.0
64	3491.0
65	3215.0
66	2601.5
67	1988.0
68	1664.0
69	1340.0
70	1069.0
71	798.0
72	642.5
73	487.0
74	436.0
75	299.0
76	213.0
77	150.0
78	87.0
79	75.5
80	64.0
81	49.5
82	35.0
83	23.0
84	11.0
85	9.0
86	7.0
87	3.5
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	319283.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.445950135371874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.29642561886205	29.64543575681488
2	10.738812852810758	8.686829783157489
3	4.349205816145686	5.277232847048931
4	2.524680047002979	4.084523331554032
5	1.6849132994213534	3.4073959645410477
6	1.3556865564059353	3.289921851575308
7	1.039254875665814	2.942355561538513
8	0.771734224295839	2.4970819162903517
9	0.6872682438565986	2.5017495408583725
>10	3.3760986830687076	21.168016093344534
>50	0.09851507818000825	2.8922671193688023
>100	0.06333112168714816	4.783656758658634
>500	0.007036791298572018	1.822134609862567
>1k	0.006254925598730683	4.2495392029387675
>5k	7.818656998413354E-4	2.7518596624477714
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8702	2.725481782619181	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3086	0.9665406551554577	No Hit
CCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC	2286	0.7159792409868362	No Hit
GCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC	1899	0.5947701568827655	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCC	1868	0.5850609020837313	No Hit
CTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGCT	1220	0.3821061566071479	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1062	0.33262027730884514	No Hit
TCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC	1009	0.31602058362017393	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1008	0.31570738185246316	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	888	0.27812316972716994	No Hit
ACTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTGC	695	0.21767522855899	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	680	0.21297720204332835	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTC	655	0.2051471578505589	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCG	609	0.19073987653586316	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCG	606	0.18980027123273085	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGC	571	0.17883820936285363	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	556	0.174140182847192	No Hit
TAAAACGGGAATTTTTCCTTTTATATGCTGTCTCTTATACACATCTGACGC	502	0.15722728739081004	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	473	0.1481444361271975	No Hit
ACCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTG	458	0.14344640961153585	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGC	404	0.1265335141551539	No Hit
CGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTG	396	0.12402790001346767	No Hit
GCCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTG	394	0.12340149647804613	No Hit
GGTTGGGAGAACTGTATGATTTTAATAATTGATGAATAACACTAGTATATT	389	0.12183548763949224	No Hit
GGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTG	364	0.11400544344672282	No Hit
AGCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCTG	355	0.11118662753732582	No Hit
GGCCTGTCTCTTATACACATCTGACGCCGCTTTCATCGTATGCCGTCTTCT	323	0.10116417097058095	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.40559628918545615	0.0
2	0.0	0.0	0.0	2.367492162125763	0.0
3	0.0	0.0	0.0	3.3005202281361674	0.0
4	0.0	0.0	0.0	4.526391946956148	0.0
5	0.0	0.0	0.0	7.78431673468365	0.0
6	0.0	0.0	0.0	9.49345878108136	0.0
7	0.0	0.0	0.0	11.300006577237122	0.0
8	0.0	0.0	0.0	13.796537867659724	0.0
9	0.0	0.0	0.0	14.711400231142905	0.0
10	0.0	0.0	0.0	17.180996169542382	0.0
11	0.0	0.0	0.0	21.38134507631161	0.0
12	0.0	0.0	0.0	24.409692968307112	0.0
13	0.0	0.0	0.0	25.483035426251945	0.0
14	0.0	0.0	0.0	25.878609258870657	0.0
15	0.0	0.0	0.0	26.743985743055532	0.0
16	0.0	0.0	0.0	28.50042125637757	0.0
17	0.0	0.0	0.0	30.48737327073474	0.0
18	0.0	0.0	0.0	32.598353185105374	0.0
19	0.0	0.0	0.0	33.90534416176245	0.0
20	0.0	0.0	0.0	35.185399786396395	0.0
21	0.0	0.0	0.0	36.61610546129922	0.0
22	0.0	0.0	0.0	38.02582661776543	0.0
23	0.0	0.0	0.0	39.26078118784903	0.0
24	0.0	0.0	0.0	40.10830517127439	0.0
25	0.0	0.0	0.0	40.84495572893013	0.0
26	0.0	0.0	0.0	41.52053194188228	0.0
27	0.0	0.0	0.0	42.04577130633325	0.0
28	3.1320176771077693E-4	0.0	0.0	42.751414888985636	0.0
29	3.1320176771077693E-4	0.0	0.0	43.283231490558535	0.0
30	3.1320176771077693E-4	0.0	0.0	43.90055217471647	0.0
31	6.264035354215539E-4	0.0	0.0	44.36127197501903	0.0
32	6.264035354215539E-4	0.0	0.0	44.80351287102664	0.0
33	6.264035354215539E-4	0.0	0.0	45.18092100111813	0.0
34	6.264035354215539E-4	0.0	0.0	45.545801060501184	0.0
35	6.264035354215539E-4	0.0	0.0	45.929473225946886	0.0
36	6.264035354215539E-4	0.0	0.0	46.284017626995485	0.0
37	6.264035354215539E-4	0.0	0.0	46.621962334355416	0.0
38	6.264035354215539E-4	0.0	0.0	46.98277077075823	0.0
39	6.264035354215539E-4	0.0	0.0	47.26778437937504	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCTAGG	40	6.7884685E-9	45.000004	2
CGGGTCT	20	7.025624E-4	45.000004	6
CGGGTAT	20	7.025624E-4	45.000004	6
GCGACGG	20	7.025624E-4	45.000004	2
TTAGCGG	20	7.025624E-4	45.000004	2
ATTACGG	20	7.025624E-4	45.000004	2
ATTCGAC	40	6.7884685E-9	45.000004	9
CGCGAGG	20	7.025624E-4	45.000004	2
AATGCGG	20	7.025624E-4	45.000004	2
CGGGCTA	20	7.025624E-4	45.000004	6
TATTACG	20	7.025624E-4	45.000004	1
CGACTGA	40	6.7884685E-9	45.000004	12
GGATTCG	40	6.7884685E-9	45.000004	7
GATTCGA	40	6.7884685E-9	45.000004	8
TTCGACT	40	6.7884685E-9	45.000004	10
TCGCGGG	20	7.025624E-4	45.000004	3
TGACTAC	40	6.7884685E-9	45.000004	16
AAGTAGT	70	0.0	45.0	4
TCCGCTT	25	3.8844562E-5	45.0	27
CACGCGG	35	1.2082455E-7	45.0	37
>>END_MODULE