SRR2935252.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935252.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	600533
Sequences flagged as poor quality	0
Sequence length	51
%GC	39

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18064	3.0079945648282442	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGC	6509	1.0838704950435696	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCG	5854	0.9748007186948926	No Hit
GCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGC	5191	0.8643987924060792	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTC	4676	0.7786416400097913	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCC	4651	0.7744786714468647	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3776	0.6287747717444337	No Hit
CCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGC	3717	0.618950165935927	No Hit
CTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGCT	2550	0.4246227934185132	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGAAATGGT	1809	0.3012324052133688	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCG	1675	0.2789188937160822	No Hit
TCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGC	1432	0.23845483928443567	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCT	1425	0.23728920808681622	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGC	1172	0.19515996622999904	No Hit
GAACTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCT	1095	0.1823380230561851	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGAAATGGTCGTA	1090	0.18150542934359976	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1089	0.1813389106010827	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1057	0.17601031084053664	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1049	0.17467816090040014	No Hit
ACTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGC	1005	0.16735133622964934	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	980	0.16318836766672273	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGT	872	0.14520434347487982	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTC	735	0.12239127575004204	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTC	726	0.12089260706738847	No Hit
CGCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTG	686	0.11423185736670592	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	686	0.11423185736670592	No Hit
GCCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTG	602	0.10024428299527254	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGGT	25	3.888572E-5	45.000004	38
TTCGTAG	25	3.888572E-5	45.000004	1
CTAACGG	25	3.888572E-5	45.000004	2
CATACTC	25	3.888572E-5	45.000004	10
GCAATTG	25	3.888572E-5	45.000004	1
GCGCTAG	25	3.888572E-5	45.000004	1
TCGAAGG	25	3.888572E-5	45.000004	2
GCGTAAG	25	3.888572E-5	45.000004	1
AGTAACG	25	3.888572E-5	45.000004	1
CGAACGA	20	7.030592E-4	45.0	3
GCGACCG	20	7.030592E-4	45.0	33
GAGTACG	20	7.030592E-4	45.0	1
CAATTGT	40	6.8048394E-9	45.0	26
TACGGGT	70	0.0	45.0	4
CGCGAGG	20	7.030592E-4	45.0	2
ATCGTAG	20	7.030592E-4	45.0	1
ACTAGCG	20	7.030592E-4	45.0	1
TCTACGG	55	1.8189894E-12	45.0	2
TATTACG	20	7.030592E-4	45.0	1
GCCTACC	35	1.2106284E-7	45.0	14

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content