##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935252.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	600533
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.598148644620697	31.0	31.0	34.0	30.0	34.0
2	31.713499507937115	31.0	31.0	34.0	30.0	34.0
3	31.72372209354024	31.0	31.0	34.0	30.0	34.0
4	35.532979869549216	37.0	35.0	37.0	33.0	37.0
5	33.224207495674676	37.0	35.0	37.0	30.0	37.0
6	34.11600861234936	37.0	35.0	37.0	28.0	37.0
7	35.36301918462433	37.0	35.0	37.0	32.0	37.0
8	35.22592596909745	37.0	35.0	37.0	32.0	37.0
9	37.29205389212583	39.0	37.0	39.0	34.0	39.0
10	36.78185212136552	39.0	37.0	39.0	32.0	39.0
11	36.62970894188995	39.0	35.0	39.0	32.0	39.0
12	37.006660749700686	39.0	37.0	39.0	33.0	39.0
13	36.99423012557178	39.0	37.0	39.0	33.0	39.0
14	37.97779972124763	40.0	37.0	41.0	33.0	41.0
15	38.05544907607076	40.0	37.0	41.0	33.0	41.0
16	38.10146653056535	40.0	37.0	41.0	33.0	41.0
17	38.00711534586775	40.0	37.0	41.0	33.0	41.0
18	37.902301788577816	39.0	37.0	40.0	33.0	41.0
19	37.633004347804366	39.0	37.0	40.0	33.0	41.0
20	37.31708831987584	39.0	35.0	40.0	33.0	41.0
21	37.30143722326667	39.0	35.0	40.0	33.0	41.0
22	37.26678134257401	39.0	35.0	40.0	33.0	41.0
23	36.946329344099325	39.0	35.0	40.0	32.0	41.0
24	36.679984280630705	38.0	35.0	40.0	31.0	41.0
25	36.49957454461287	38.0	35.0	40.0	31.0	41.0
26	36.78759368760751	38.0	35.0	40.0	32.0	41.0
27	36.664861048435306	38.0	35.0	40.0	32.0	41.0
28	36.676988608452824	38.0	35.0	40.0	31.0	41.0
29	36.74583411735908	39.0	35.0	40.0	32.0	41.0
30	36.4318330549695	38.0	35.0	40.0	31.0	41.0
31	36.19660368372762	38.0	35.0	40.0	30.0	41.0
32	35.79874877816873	38.0	35.0	40.0	29.0	41.0
33	35.542742863422994	38.0	35.0	40.0	27.0	41.0
34	35.30103757828462	38.0	35.0	40.0	25.0	41.0
35	35.0978114441671	38.0	35.0	40.0	23.0	41.0
36	34.79930994633101	38.0	35.0	40.0	22.0	41.0
37	34.52090559552931	38.0	34.0	40.0	21.0	41.0
38	34.31390781189377	38.0	34.0	40.0	21.0	41.0
39	34.24862413888995	38.0	33.0	40.0	20.0	41.0
40	34.28115690561551	38.0	34.0	40.0	20.0	41.0
41	34.231172974674166	38.0	34.0	40.0	19.0	41.0
42	34.20907760272957	38.0	34.0	40.0	18.0	41.0
43	33.936396501108185	38.0	33.0	40.0	18.0	41.0
44	33.7548327902047	37.0	33.0	40.0	18.0	41.0
45	33.74202083815544	37.0	33.0	40.0	18.0	41.0
46	33.68642855596612	37.0	33.0	40.0	18.0	41.0
47	33.56319136500409	37.0	33.0	40.0	18.0	41.0
48	33.48087115945335	37.0	33.0	40.0	17.0	41.0
49	33.40840220270993	36.0	33.0	40.0	17.0	41.0
50	33.20414864795107	36.0	33.0	39.0	15.0	41.0
51	32.26957053151117	35.0	31.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	11.0
11	17.0
12	17.0
13	17.0
14	43.0
15	48.0
16	92.0
17	168.0
18	316.0
19	676.0
20	1430.0
21	2267.0
22	3280.0
23	4795.0
24	6628.0
25	9276.0
26	11726.0
27	12400.0
28	12422.0
29	12057.0
30	13152.0
31	15597.0
32	19049.0
33	25124.0
34	36309.0
35	53454.0
36	69785.0
37	90021.0
38	120146.0
39	80188.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.558970114881284	19.612244456174764	20.252342502410357	19.576442926533595
2	37.31002292963084	26.771384753210896	21.98213919967762	13.936453117480637
3	23.928410262217064	26.957552707344973	36.875075974176276	12.238961056261688
4	22.016109023151103	22.90098962088678	40.714165582907185	14.368735773054937
5	20.430018000676068	30.60997480571426	33.76717016383779	15.192837029771885
6	19.11735075341405	33.763173714017384	34.70433764672383	12.415137885844741
7	60.753863651123254	7.534640061412112	26.70694199985679	5.004554287607841
8	59.26951558032615	10.649872696421346	22.729475316094202	7.351136407158308
9	54.60782338356094	8.747895619391441	24.92635708612183	11.717923910925794
10	29.121963322581774	23.696283135148278	32.239194182501215	14.94255935976874
11	19.045414656646678	24.203166187370222	36.933024496572216	19.81839465941089
12	16.664196638652665	20.44683639367029	42.16287864280564	20.726088324871405
13	19.294693214194723	22.80624045639457	44.133294923010055	13.765771406400647
14	15.690395032412873	26.701113843868697	39.215996456481165	18.392494667237273
15	11.76005315278261	25.415422632894447	44.207728800915184	18.616795413407754
16	12.796132768723783	25.442232150439693	38.03721027820286	23.72442480263366
17	12.447276003150535	26.49396452817747	39.27078112276927	21.787978345902722
18	12.644434194290739	24.465932763062145	43.21627620796859	19.673356834678525
19	12.298741284825313	27.554189361783614	40.20095481846959	19.946114534921477
20	13.304681008370897	28.887671451860264	42.29009896208868	15.51754857768016
21	13.494512374840351	30.70056100164354	40.073234942959004	15.731691680557105
22	11.077992383432719	27.916534145500748	37.816572944367756	23.188900526698784
23	11.524262613378449	29.331277381925723	36.877407236571514	22.267052768124316
24	14.38322290365392	27.34054581513422	35.78870769799495	22.48752358321691
25	11.227692732955557	32.27982475567537	34.13634221599812	22.356140295370945
26	13.462041220049523	28.604922627066287	37.86852679203307	20.06450936085111
27	16.85436104260715	28.39843938634513	36.01434059410557	18.73285897694215
28	11.634664539667263	29.24701889821209	37.68202580041397	21.436290761706683
29	17.39088443099713	27.085772139083115	35.011398207925296	20.511945221994463
30	19.18429128790591	31.858865374592238	32.475484278132924	16.481359059368927
31	16.582435936076788	33.72753870311873	30.157376863552877	19.53264849725161
32	20.14327272606168	33.936353206235125	27.403323381063156	18.517050686640037
33	19.796247666656118	30.656267016134002	28.179467239935192	21.368018077274687
34	19.984913401927955	28.212437950953568	31.2905369063815	20.51211174073698
35	24.982806939835115	27.627957164718676	27.319231416092038	20.07000447935417
36	27.09726193231679	31.007788081587524	26.785705365067365	15.10924462102832
37	21.228808408530423	33.573508866290446	30.781988666734385	14.415694058444748
38	18.471258032447842	38.22221260113932	25.363801822714155	17.94272754369868
39	20.322280374267525	33.25778933047809	30.55519013942614	15.864740155828239
40	23.132117635500464	28.03426289646031	32.374573920167585	16.45904554787164
41	26.058684535237862	26.558240762789055	28.52532666814313	18.857748033829946
42	28.211938394726015	24.82927665923438	30.101926122294692	16.856858823744908
43	26.311293467636247	26.70194643758128	29.20988521863078	17.776874876151684
44	20.54724719540808	27.761338677474846	29.785207474027235	21.90620665308984
45	21.076776796612343	26.183740110868182	29.892112506723194	22.847370585796284
46	24.605975025518998	27.966656286998383	31.57228661872037	15.85508206876225
47	18.853252027781984	27.97115229304634	37.05558229106477	16.120013388106898
48	20.92541125966433	26.54924875069313	32.91209642101266	19.61324356862987
49	22.506506719863854	23.115798798733792	35.62851666769353	18.749177813708823
50	21.56251196853462	21.872403348358873	32.51678092627716	24.048303756829352
51	19.820226365578577	21.507227746018952	29.504290355400954	29.16825553300152
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4912.0
1	5864.0
2	6816.0
3	17164.0
4	27512.0
5	17419.0
6	7326.0
7	7387.5
8	7449.0
9	7853.5
10	8258.0
11	8779.0
12	9300.0
13	9151.5
14	9003.0
15	8644.5
16	8286.0
17	7717.0
18	7148.0
19	7058.5
20	6969.0
21	6959.0
22	6949.0
23	6863.0
24	6777.0
25	7065.0
26	7611.5
27	7870.0
28	7975.5
29	8081.0
30	8914.5
31	9748.0
32	9611.5
33	9475.0
34	10033.5
35	10592.0
36	11227.0
37	11862.0
38	12771.0
39	13680.0
40	15618.5
41	17557.0
42	22502.0
43	27447.0
44	31875.5
45	36304.0
46	54982.0
47	73660.0
48	74819.0
49	75978.0
50	75291.0
51	74604.0
52	57531.5
53	40459.0
54	30759.0
55	21059.0
56	16464.0
57	11869.0
58	9786.5
59	7704.0
60	6885.0
61	6066.0
62	5183.0
63	4300.0
64	3614.0
65	2928.0
66	2492.0
67	2056.0
68	1645.0
69	1234.0
70	1008.5
71	783.0
72	607.0
73	431.0
74	395.5
75	251.5
76	143.0
77	132.5
78	122.0
79	84.5
80	47.0
81	37.5
82	28.0
83	17.0
84	6.0
85	5.0
86	4.0
87	8.5
88	13.0
89	7.5
90	2.0
91	2.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	600533.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.66678715700461
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.33686821096869	30.63708384610771
2	10.514569622993745	8.551875298115858
3	4.358230399592853	5.317056841242892
4	2.245412113928253	3.6525478606752024
5	1.441370471567268	2.9307953090808727
6	0.9571766273778171	2.3355178906339926
7	0.7673460384282175	2.18438486143717
8	0.5967427143531808	1.9414087161675204
9	0.5040296098632154	1.844753837862191
>10	3.117315180150319	21.36743516518228
>50	0.09622724144277073	2.649733497040326
>100	0.05252578679680399	3.7283558250965507
>500	0.003781856649369888	1.0875083174862246
>1k	0.006723300709990912	5.684982197695116
>5k	0.0012606188831232959	2.999704746252857
>10k+	4.20206294374432E-4	3.0868557899231868
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18064	3.0079945648282442	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGC	6509	1.0838704950435696	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCG	5854	0.9748007186948926	No Hit
GCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGC	5191	0.8643987924060792	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTC	4676	0.7786416400097913	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCC	4651	0.7744786714468647	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3776	0.6287747717444337	No Hit
CCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGC	3717	0.618950165935927	No Hit
CTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGCT	2550	0.4246227934185132	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGAAATGGT	1809	0.3012324052133688	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCG	1675	0.2789188937160822	No Hit
TCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGC	1432	0.23845483928443567	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCT	1425	0.23728920808681622	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGC	1172	0.19515996622999904	No Hit
GAACTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCT	1095	0.1823380230561851	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGAAATGGTCGTA	1090	0.18150542934359976	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1089	0.1813389106010827	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1057	0.17601031084053664	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1049	0.17467816090040014	No Hit
ACTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGC	1005	0.16735133622964934	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	980	0.16318836766672273	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGT	872	0.14520434347487982	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTC	735	0.12239127575004204	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTC	726	0.12089260706738847	No Hit
CGCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTG	686	0.11423185736670592	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	686	0.11423185736670592	No Hit
GCCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTG	602	0.10024428299527254	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.66518742517064E-4	0.0	0.0	0.4505997172511752	0.0
2	1.66518742517064E-4	0.0	0.0	2.492286019252897	0.0
3	1.66518742517064E-4	0.0	0.0	3.1933299252497367	0.0
4	1.66518742517064E-4	0.0	0.0	4.243397115562342	0.0
5	1.66518742517064E-4	0.0	0.0	7.326824670750816	0.0
6	1.66518742517064E-4	0.0	0.0	8.777868993044512	0.0
7	1.66518742517064E-4	0.0	0.0	10.448551536718215	0.0
8	1.66518742517064E-4	0.0	0.0	12.752504858184313	0.0
9	1.66518742517064E-4	0.0	0.0	13.764106218975476	0.0
10	3.33037485034128E-4	0.0	0.0	17.771712795133656	0.0
11	3.33037485034128E-4	0.0	0.0	22.704830542201677	0.0
12	3.33037485034128E-4	0.0	0.0	27.196507102856962	0.0
13	3.33037485034128E-4	0.0	0.0	28.617578051497585	0.0
14	3.33037485034128E-4	0.0	0.0	29.16975420168417	0.0
15	3.33037485034128E-4	0.0	0.0	30.31840048756688	0.0
16	3.33037485034128E-4	0.0	0.0	32.221376677051886	0.0
17	3.33037485034128E-4	0.0	0.0	34.60975500097413	0.0
18	3.33037485034128E-4	0.0	0.0	36.89589081699091	0.0
19	3.33037485034128E-4	0.0	0.0	38.67547661827077	0.0
20	3.33037485034128E-4	0.0	0.0	39.93702261158005	0.0
21	3.33037485034128E-4	0.0	0.0	41.363921716208765	0.0
22	3.33037485034128E-4	0.0	0.0	42.749024616465704	0.0
23	3.33037485034128E-4	0.0	0.0	44.02222692175118	0.0
24	3.33037485034128E-4	0.0	0.0	44.90910574439706	0.0
25	3.33037485034128E-4	0.0	0.0	45.56419047745919	0.0
26	3.33037485034128E-4	0.0	0.0	46.14550740758626	0.0
27	3.33037485034128E-4	0.0	0.0	46.662048546874196	0.0
28	3.33037485034128E-4	0.0	0.0	47.123638501131495	0.0
29	3.33037485034128E-4	0.0	0.0	47.58273067425104	0.0
30	3.33037485034128E-4	0.0	0.0	48.0389920287478	0.0
31	3.33037485034128E-4	0.0	0.0	48.43380796725575	0.0
32	3.33037485034128E-4	0.0	0.0	48.78516251396676	0.0
33	3.33037485034128E-4	0.0	0.0	49.10654368702469	0.0
34	3.33037485034128E-4	0.0	0.0	49.40244749247751	0.0
35	3.33037485034128E-4	0.0	0.0	49.72266303433783	0.0
36	3.33037485034128E-4	0.0	0.0	50.026892776916505	0.0
37	3.33037485034128E-4	0.0	0.0	50.314470645243475	0.0
38	3.33037485034128E-4	0.0	0.0	50.57024343374969	0.0
39	3.33037485034128E-4	0.0	0.0	50.83350956566916	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGGT	25	3.888572E-5	45.000004	38
TTCGTAG	25	3.888572E-5	45.000004	1
CTAACGG	25	3.888572E-5	45.000004	2
CATACTC	25	3.888572E-5	45.000004	10
GCAATTG	25	3.888572E-5	45.000004	1
GCGCTAG	25	3.888572E-5	45.000004	1
TCGAAGG	25	3.888572E-5	45.000004	2
GCGTAAG	25	3.888572E-5	45.000004	1
AGTAACG	25	3.888572E-5	45.000004	1
CGAACGA	20	7.030592E-4	45.0	3
GCGACCG	20	7.030592E-4	45.0	33
GAGTACG	20	7.030592E-4	45.0	1
CAATTGT	40	6.8048394E-9	45.0	26
TACGGGT	70	0.0	45.0	4
CGCGAGG	20	7.030592E-4	45.0	2
ATCGTAG	20	7.030592E-4	45.0	1
ACTAGCG	20	7.030592E-4	45.0	1
TCTACGG	55	1.8189894E-12	45.0	2
TATTACG	20	7.030592E-4	45.0	1
GCCTACC	35	1.2106284E-7	45.0	14
>>END_MODULE