FastQCFastQC Report
Sat 14 Jan 2017
SRR2935250.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935250.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences637210
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264854.15640055868552No Hit
GAATCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTC94731.4866370584265785No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCG74561.1701009086486402No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGC72151.1322797821754211No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA51070.8014626261358108No Hit
GCTCAACTTGACCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATG46330.7270758462673216No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCC43240.6785831986315344No Hit
GCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC34920.5480139985248191No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32360.5078388600304452No Hit
GCTCAACTTGACCCCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTA30890.48476954222312896No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT25340.39767109744040424No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTATAGCGT23750.3727185700161642No Hit
CCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC22420.351846330095259No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCT20480.32140110795499127No Hit
GAACTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCT20250.3177916228558874No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19700.3091602454449867No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17850.28012743051741185No Hit
GCTCAACTTGACCCTGCATTAGAACTGTCTCTTATACACATCTGACGCCTA17070.2678865680074073No Hit
TGGTATAGAAAAGATTTTCTTTCCTTTTTACTTAATGTATATAGTTTAACT16890.26506175358202166No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16770.2631785439650979No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16730.26255080742612324No Hit
GCTCAACCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTC16120.2529778252067607No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTT15780.24764206462547667No Hit
CTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGCT15480.24293404058316725No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTC15130.23744134586713958No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCG13050.20479904584046077No Hit
GCTCAACTTGACCCTGCATCTGTCTCTTATACACATCTGACGCCTATAGCG12880.20213116554981875No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11970.18785015928814677No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTATAGCGTCGTA11840.18581001553647933No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGC10320.16195602705544485No Hit
GCTCCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTC10230.16054361984275198No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10190.1599158833037774No Hit
TCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC9300.14594874531159271No Hit
GCTCAACTTCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCG8140.12774438568132956No Hit
GCCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTG7580.11895607413568525No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACCTGTCTCTTATACACATC7470.11722979865350512No Hit
ACTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC6740.1057736068172188No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGT6680.10483200200875693No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGCG302.1639735E-645.0000041
CCCGTCG302.1639735E-645.00000440
TCTCACG302.1639735E-645.00000423
GTCGATG253.8888422E-545.01
TAGACGT207.0309173E-445.08
CGACCCG453.8380676E-1045.032
GCGTTTA207.0309173E-445.032
CGTTATT5550.045.01
GTAATAC207.0309173E-445.026
CACCTAA207.0309173E-445.031
ACGATTT207.0309173E-445.018
CGAATGC800.045.045
TAGTGCG253.8888422E-545.01
ATCATAC253.8888422E-545.025
CGTTTAC207.0309173E-445.033
TCGAATG850.045.044
GCCCGAA207.0309173E-445.025
CTTGACC24450.044.5398757
ACTTGAC24400.044.5389376
GCTCAAC27500.044.3454551
Produced by FastQC (version 0.11.5)