##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935250.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	637210
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.6070400652846	31.0	31.0	34.0	30.0	34.0
2	31.744100061204314	31.0	31.0	34.0	30.0	34.0
3	31.765305001490873	31.0	31.0	34.0	30.0	34.0
4	35.56197328981027	37.0	35.0	37.0	33.0	37.0
5	33.00632130694748	37.0	35.0	37.0	28.0	37.0
6	34.01952260636211	37.0	35.0	37.0	28.0	37.0
7	35.28094348801808	37.0	35.0	37.0	32.0	37.0
8	35.12149683777719	37.0	35.0	37.0	32.0	37.0
9	37.186684138667005	39.0	37.0	39.0	34.0	39.0
10	36.86915302647479	39.0	37.0	39.0	32.0	39.0
11	36.679579730387154	39.0	37.0	39.0	32.0	39.0
12	36.993187489210776	39.0	37.0	39.0	33.0	39.0
13	36.944779586007755	39.0	37.0	39.0	33.0	39.0
14	37.956505704555795	40.0	37.0	41.0	33.0	41.0
15	38.015845639585066	40.0	37.0	41.0	33.0	41.0
16	38.05119034541203	40.0	37.0	41.0	33.0	41.0
17	37.88835391786068	40.0	37.0	41.0	33.0	41.0
18	37.69832394344094	39.0	37.0	40.0	33.0	41.0
19	37.465162191428256	39.0	36.0	40.0	33.0	41.0
20	37.03050799579417	39.0	35.0	40.0	32.0	41.0
21	37.017692754350996	39.0	35.0	40.0	32.0	41.0
22	37.02615464289638	39.0	35.0	40.0	32.0	41.0
23	36.632596789127604	38.0	35.0	40.0	31.0	41.0
24	36.38220209977872	38.0	35.0	40.0	31.0	41.0
25	36.261246684766405	38.0	35.0	40.0	31.0	41.0
26	36.507623860265845	38.0	35.0	40.0	31.0	41.0
27	36.389058552125675	38.0	35.0	40.0	31.0	41.0
28	36.372735832770985	38.0	35.0	40.0	31.0	41.0
29	36.47367743757945	38.0	35.0	40.0	31.0	41.0
30	36.18646756956105	38.0	35.0	40.0	31.0	41.0
31	36.050072974372654	38.0	35.0	40.0	30.0	41.0
32	35.72247610677798	38.0	35.0	40.0	29.0	41.0
33	35.45150578302287	38.0	35.0	40.0	26.0	41.0
34	35.20686900707773	38.0	35.0	40.0	24.0	41.0
35	34.920326109132	38.0	35.0	40.0	22.0	41.0
36	34.70377583528193	38.0	35.0	40.0	21.0	41.0
37	34.57096090770703	38.0	35.0	40.0	20.0	41.0
38	34.437052149212974	38.0	34.0	40.0	20.0	41.0
39	34.312667723356505	38.0	34.0	40.0	18.0	41.0
40	34.22443778346228	38.0	34.0	40.0	18.0	41.0
41	33.994199714379874	38.0	33.0	40.0	18.0	41.0
42	34.12624409535318	38.0	34.0	40.0	18.0	41.0
43	34.0172360760189	38.0	34.0	40.0	17.0	41.0
44	33.987465670658025	38.0	34.0	40.0	16.0	41.0
45	33.973522072786054	38.0	34.0	40.0	18.0	41.0
46	33.87479323927747	38.0	33.0	40.0	17.0	41.0
47	33.728593399350295	37.0	33.0	40.0	16.0	41.0
48	33.6664239418716	37.0	33.0	40.0	17.0	41.0
49	33.70890130412266	37.0	33.0	40.0	17.0	41.0
50	33.61479104219959	37.0	33.0	40.0	15.0	41.0
51	32.669019632460255	36.0	31.0	40.0	13.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	17.0
10	18.0
11	22.0
12	22.0
13	28.0
14	54.0
15	67.0
16	116.0
17	196.0
18	431.0
19	917.0
20	1662.0
21	2808.0
22	4258.0
23	5895.0
24	7779.0
25	10042.0
26	12848.0
27	13487.0
28	13518.0
29	13213.0
30	14117.0
31	16613.0
32	20293.0
33	26373.0
34	38417.0
35	56286.0
36	71070.0
37	82768.0
38	120874.0
39	102981.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.51629760989313	18.23449098413396	19.476153858225704	20.77305754774721
2	36.23781798779052	27.591688768223975	19.21407385320381	16.956419390781686
3	22.359818584140235	27.433656094537124	38.51822789975047	11.688297421572166
4	20.051788264465404	20.20778079440059	41.94362925880008	17.796801682333925
5	21.578914329655845	31.96638470833791	31.932957737637512	14.521743224368732
6	17.801980508780467	36.1802231603396	35.36055617457353	10.657240156306399
7	53.369532807080866	7.637042733164891	30.153010781375055	8.840413678379184
8	51.15111187834466	12.524913293890554	29.77071922913953	6.553255598625257
9	46.0137160433766	9.274807363349602	30.80773999152556	13.903736601748246
10	31.970778864110734	22.320585050454326	33.141664443433086	12.566971642001851
11	20.620988371180616	24.289951507352363	37.82175420975816	17.267305911708856
12	16.48075202837369	18.495943252616875	41.75656377018565	23.266740948823777
13	19.634971202586275	19.227256320522276	44.58043659076286	16.55733588612859
14	14.782881624582162	25.831986315343453	39.922003735032405	19.463128325041982
15	12.674942326705482	21.64937775615574	47.155098005367144	18.52058191177163
16	15.279735095180552	23.647149291442382	39.68393465262629	21.38918096075077
17	13.298441644041995	25.333406569262877	39.1884935892406	22.179658197454525
18	13.048288633260622	25.400417444798418	42.35055947019036	19.200734451750602
19	12.968879961080335	24.943425244424915	40.26333547809984	21.824359316394908
20	15.109932361387926	26.26465372483169	43.99114891480046	14.63426499897993
21	14.316316441989297	29.96986864612922	40.75924734388977	14.954567567991713
22	15.07870246857394	23.517050893739896	40.62036063464164	20.783886003044522
23	13.9481489618807	27.275466486715526	40.01443794039641	18.76194661100736
24	15.672070432039673	27.241882581880382	36.72949263194237	20.356554354137568
25	12.533230803031966	30.953061000298177	35.64350841951633	20.870199777153527
26	12.646380314182137	24.93620627422671	41.05396964893834	21.363443762652814
27	16.887368371494485	23.682616405894446	39.63889455595487	19.791120666656205
28	12.743836411857941	26.456427237488427	39.36598609563566	21.43375025501797
29	13.450353886473849	23.51579542066195	39.924043878784076	23.10980681408013
30	14.34786020307277	29.409142982690167	38.587121984902936	17.655874829334127
31	16.268420144065534	28.086972897474933	33.66629525588111	21.97831170257843
32	16.46301847114766	27.742502471712623	35.12970606236562	20.664772994774093
33	16.695124056433514	27.03676966777044	33.72294847852356	22.545157797272488
34	18.56923149354216	24.923651543447214	33.270350433922886	23.236766529087742
35	17.855181180458562	24.39603898243907	33.824641797837444	23.924138039264918
36	18.43709295208801	27.618210636995656	32.7300262080005	21.214670202915837
37	17.101269597150075	26.48247830385587	37.11131338177367	19.30493871722038
38	15.754460852780088	29.504872804883792	32.266756642237254	22.47390970009887
39	21.27760079094804	26.627328510224256	32.8609092763767	19.234161422450995
40	18.974749297719747	23.205379702139012	36.22824500557116	21.59162599457008
41	20.102321055852858	26.809529040661634	29.381522574975282	23.706627328510223
42	19.64250404105397	21.135418464870295	35.41705246308125	23.805025030994493
43	20.616123413003564	23.797806060796283	33.19408044443747	22.391990081762682
44	19.89202931529637	23.03965725584972	33.93904678206557	23.129266646788345
45	18.236060325481397	22.21763625806249	33.930729272924154	25.615574143531962
46	22.18828957486543	23.565857409645172	33.6418135308611	20.604039484628302
47	16.30043470755324	23.546240642802214	40.35200326423	19.801321385414543
48	17.553867641750756	23.760455736727295	34.97120258627454	23.714474035247406
49	18.98008505830103	21.391378038637185	38.00018832096169	21.62834858210009
50	18.642205866197955	19.7702484267353	37.77090755010122	23.81663815696552
51	18.194786648043817	20.593681831735218	32.92619387643006	28.285337643790903
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5054.0
1	7646.0
2	10238.0
3	24139.5
4	38041.0
5	23382.5
6	8724.0
7	8755.0
8	8786.0
9	9289.0
10	9792.0
11	9889.0
12	9986.0
13	9616.5
14	9247.0
15	8828.5
16	8410.0
17	7484.5
18	6559.0
19	6170.0
20	5781.0
21	6695.0
22	7609.0
23	6850.0
24	6091.0
25	6478.5
26	6902.0
27	6938.0
28	7387.0
29	7836.0
30	11060.0
31	14284.0
32	12242.0
33	10200.0
34	10823.5
35	11447.0
36	12135.5
37	12824.0
38	15141.0
39	17458.0
40	18551.0
41	19644.0
42	23065.0
43	26486.0
44	31060.5
45	35635.0
46	58337.0
47	81039.0
48	73851.5
49	66664.0
50	62410.0
51	58156.0
52	45788.5
53	33421.0
54	27465.5
55	21510.0
56	18679.0
57	15848.0
58	14073.5
59	12299.0
60	11435.5
61	10572.0
62	10015.0
63	9458.0
64	9055.0
65	8652.0
66	7570.0
67	6488.0
68	5264.5
69	4041.0
70	3074.0
71	2107.0
72	1713.0
73	1319.0
74	1043.5
75	608.0
76	448.0
77	356.0
78	264.0
79	187.5
80	111.0
81	91.5
82	72.0
83	46.0
84	20.0
85	14.5
86	9.0
87	5.5
88	2.0
89	2.5
90	3.0
91	1.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	637210.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.229478933532334
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.79609630115499	27.846196915543054
2	9.905797680007916	7.375753720953764
3	4.009681707895667	4.47835082022815
4	2.2952198699345896	3.4179935918221847
5	1.611120787592845	2.999059371053192
6	1.2554986871324432	2.8044937154204876
7	1.0350392998825197	2.697378166724803
8	0.8344303498850328	2.4852325706035874
9	0.6838091577804961	2.291207277073092
>10	3.422271615872467	19.195674071808252
>50	0.08152432711875093	2.186555857913147
>100	0.051489048697977755	3.4499595892427175
>500	0.004290754058164812	1.1151626565033759
>1k	0.011585035957044994	8.753571587318723
>5k	0.0017163016232659251	4.672628973697214
>10k+	4.290754058164813E-4	4.230781114094243
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26485	4.15640055868552	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTC	9473	1.4866370584265785	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCG	7456	1.1701009086486402	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGC	7215	1.1322797821754211	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	5107	0.8014626261358108	No Hit
GCTCAACTTGACCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATG	4633	0.7270758462673216	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCC	4324	0.6785831986315344	No Hit
GCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC	3492	0.5480139985248191	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3236	0.5078388600304452	No Hit
GCTCAACTTGACCCCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTA	3089	0.48476954222312896	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT	2534	0.39767109744040424	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTATAGCGT	2375	0.3727185700161642	No Hit
CCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC	2242	0.351846330095259	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCT	2048	0.32140110795499127	No Hit
GAACTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCT	2025	0.3177916228558874	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1970	0.3091602454449867	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1785	0.28012743051741185	No Hit
GCTCAACTTGACCCTGCATTAGAACTGTCTCTTATACACATCTGACGCCTA	1707	0.2678865680074073	No Hit
TGGTATAGAAAAGATTTTCTTTCCTTTTTACTTAATGTATATAGTTTAACT	1689	0.26506175358202166	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1677	0.2631785439650979	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1673	0.26255080742612324	No Hit
GCTCAACCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTC	1612	0.2529778252067607	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTT	1578	0.24764206462547667	No Hit
CTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGCT	1548	0.24293404058316725	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTC	1513	0.23744134586713958	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCG	1305	0.20479904584046077	No Hit
GCTCAACTTGACCCTGCATCTGTCTCTTATACACATCTGACGCCTATAGCG	1288	0.20213116554981875	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1197	0.18785015928814677	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTATAGCGTCGTA	1184	0.18581001553647933	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGC	1032	0.16195602705544485	No Hit
GCTCCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTC	1023	0.16054361984275198	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1019	0.1599158833037774	No Hit
TCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC	930	0.14594874531159271	No Hit
GCTCAACTTCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCG	814	0.12774438568132956	No Hit
GCCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTG	758	0.11895607413568525	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACCTGTCTCTTATACACATC	747	0.11722979865350512	No Hit
ACTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC	674	0.1057736068172188	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGT	668	0.10483200200875693	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.708024042309443E-4	0.0	0.0	0.2614522684829177	0.0
2	4.708024042309443E-4	0.0	0.0	1.4883633339087585	0.0
3	4.708024042309443E-4	0.0	0.0	2.089734938246418	0.0
4	4.708024042309443E-4	0.0	0.0	3.0850112207906344	0.0
5	4.708024042309443E-4	0.0	0.0	6.5634563173835945	0.0
6	4.708024042309443E-4	0.0	0.0	7.920308846377176	0.0
7	4.708024042309443E-4	0.0	0.0	9.372891197564382	0.0
8	4.708024042309443E-4	0.0	0.0	11.422450997316426	0.0
9	4.708024042309443E-4	0.0	0.0	12.16255237676747	0.0
10	4.708024042309443E-4	0.0	0.0	15.13551262535114	0.0
11	4.708024042309443E-4	0.0	0.0	18.057940082547354	0.0
12	4.708024042309443E-4	0.0	0.0	21.262378179877906	0.0
13	4.708024042309443E-4	0.0	0.0	22.87016839032658	0.0
14	4.708024042309443E-4	0.0	0.0	23.233470912258124	0.0
15	4.708024042309443E-4	0.0	0.0	24.550776039296306	0.0
16	4.708024042309443E-4	0.0	0.0	25.75838420614868	0.0
17	4.708024042309443E-4	0.0	0.0	27.177853454904977	0.0
18	4.708024042309443E-4	0.0	0.0	28.553067277663565	0.0
19	4.708024042309443E-4	0.0	0.0	29.99011314951115	0.0
20	4.708024042309443E-4	0.0	0.0	31.11407542254516	0.0
21	4.708024042309443E-4	0.0	0.0	32.11437359740117	0.0
22	4.708024042309443E-4	0.0	0.0	33.15045275497874	0.0
23	4.708024042309443E-4	0.0	0.0	34.0653787605342	0.0
24	4.708024042309443E-4	0.0	0.0	34.73689992310227	0.0
25	4.708024042309443E-4	0.0	0.0	35.531457447309364	0.0
26	4.708024042309443E-4	0.0	0.0	36.0137160433766	0.0
27	4.708024042309443E-4	0.0	0.0	36.48404764520331	0.0
28	4.708024042309443E-4	0.0	0.0	36.884229688799614	0.0
29	4.708024042309443E-4	0.0	0.0	37.29351391221105	0.0
30	4.708024042309443E-4	0.0	0.0	37.718020746692616	0.0
31	4.708024042309443E-4	0.0	0.0	38.08414808304954	0.0
32	4.708024042309443E-4	0.0	0.0	38.532351971877404	0.0
33	4.708024042309443E-4	0.0	0.0	38.86458153512971	0.0
34	4.708024042309443E-4	0.0	0.0	39.306664992702565	0.0
35	6.277365389745923E-4	0.0	0.0	39.65741278385462	0.0
36	6.277365389745923E-4	0.0	0.0	39.94774093313036	0.0
37	6.277365389745923E-4	0.0	0.0	40.26270774156087	0.0
38	6.277365389745923E-4	0.0	0.0	40.585992059132785	0.0
39	6.277365389745923E-4	0.0	0.0	40.950079251738046	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAGCG	30	2.1639735E-6	45.000004	1
CCCGTCG	30	2.1639735E-6	45.000004	40
TCTCACG	30	2.1639735E-6	45.000004	23
GTCGATG	25	3.8888422E-5	45.0	1
TAGACGT	20	7.0309173E-4	45.0	8
CGACCCG	45	3.8380676E-10	45.0	32
GCGTTTA	20	7.0309173E-4	45.0	32
CGTTATT	555	0.0	45.0	1
GTAATAC	20	7.0309173E-4	45.0	26
CACCTAA	20	7.0309173E-4	45.0	31
ACGATTT	20	7.0309173E-4	45.0	18
CGAATGC	80	0.0	45.0	45
TAGTGCG	25	3.8888422E-5	45.0	1
ATCATAC	25	3.8888422E-5	45.0	25
CGTTTAC	20	7.0309173E-4	45.0	33
TCGAATG	85	0.0	45.0	44
GCCCGAA	20	7.0309173E-4	45.0	25
CTTGACC	2445	0.0	44.539875	7
ACTTGAC	2440	0.0	44.538937	6
GCTCAAC	2750	0.0	44.345455	1
>>END_MODULE