FastQCFastQC Report
Sat 14 Jan 2017
SRR2935249.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935249.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517389
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120922.3371196527177807No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCG96651.868033529897234No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTC91501.7684952714495283No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGC71051.3732414102348525No Hit
GCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC49410.9549874465827453No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCC27120.5241704017673355No Hit
CCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC24280.4692794009922901No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23130.4470524112418316No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTCCTATT22750.4397078407155931No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCT22150.428111150411006No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA19950.3855899526275201No Hit
CTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGCT19000.36722852631192393No Hit
GAACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCT17040.3293460046502728No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG15860.3065391803845849No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTC15390.29745510631265837No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14290.2761945074209154No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTA14240.2752281165621998No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13140.25396751767045683No Hit
TCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC11680.22574890459596164No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9900.19134539002568668No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCG8800.1700847911339437No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8300.1604208825467878No Hit
ACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC8120.1569418754554117No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7920.15307631202054933No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7410.14321912526165032No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCG6630.12814342786568714No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTT6400.12369802991559543No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGC5980.11558034670238447No Hit
ACCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTG5660.1093954452066047No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5180.10011809296293504No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGA253.8878217E-545.013
AATTTGC253.8878217E-545.045
CTCGTCC207.029687E-445.037
ACGTTGG253.8878217E-545.02
CGTTGTT207.029687E-445.01
TAACGTC253.8878217E-545.032
TACGGGT253.8878217E-545.04
ACGGGTA351.2101918E-745.05
AACGAGG502.1827873E-1145.02
TCGTAAC207.029687E-445.034
CTCGAAT351.2101918E-745.043
CGTAACC207.029687E-445.035
CACAACG253.8878217E-545.012
ATTGACG253.8878217E-545.01
CGTGCGG302.1631786E-644.9999962
CGTTCTG302.1631786E-644.9999961
CGATGAA7000.043.07142619
TCGAATG950.042.6315844
CGTTATT2650.042.452831
TGGGAGT850.042.3529436
Produced by FastQC (version 0.11.5)