FastQCFastQC Report
Sat 14 Jan 2017
SRR2935248.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935248.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences368682
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81162.201355097346765No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCG30560.8288986172365345No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGC30370.8237451245246582No Hit
GCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGC28600.775736271366652TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCC26610.7217602161212102No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26490.7185053786189725No Hit
GAATCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTC25540.6927379150595906No Hit
CCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGC25520.692195442142551TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGCT16170.4385893534265302TruSeq Adapter, Index 14 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11290.3062259616688637No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAATCGCCT8730.23678942828779273No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8090.21943029494252497No Hit
TCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGC8040.21807411264992593TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCG7990.2167179303573269No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7560.20505476264097516No Hit
ACTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGC7120.19312035846610356TruSeq Adapter, Index 14 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCT6710.18199966366679143No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGC6100.16545423969708312No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAATCGCCTCGTA5480.148637579268855No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5370.1456539782251371No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5070.13751688446954285No Hit
CGCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTG5040.13670317509398344TruSeq Adapter, Index 14 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCT4750.128837317796909No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4720.12802360842134958No Hit
ACCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTG4660.1263961896702307TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTC4290.1163604407049978No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG207.027045E-445.0000041
GATAAGC207.027045E-445.0000049
GCGCGAC207.027045E-445.0000049
GATCCGG207.027045E-445.0000045
GGCCGAT302.161476E-645.0000048
ACGGGTA302.161476E-645.0000045
TCGGAGT207.027045E-445.0000042
ACGGCCA351.2089185E-745.00000430
CGGGCTA207.027045E-445.0000046
ACGTGGC207.027045E-445.00000426
CCATACG207.027045E-445.0000042
GGCGAAA207.027045E-445.00000432
AGGGCGT207.027045E-445.0000046
AGTACGG406.7939254E-945.0000042
GGCTAAA207.027045E-445.0000048
CGGACGG207.027045E-445.0000042
CGGCCAA253.8856328E-545.031
CGGGCGA253.8856328E-545.06
TTCGCAG253.8856328E-545.01
GCCAAAT253.8856328E-545.033
Produced by FastQC (version 0.11.5)