##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935248.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	368682
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.64033231890898	31.0	31.0	34.0	30.0	34.0
2	31.74309567594838	31.0	31.0	34.0	30.0	34.0
3	31.752982244861425	31.0	31.0	34.0	30.0	34.0
4	35.58074709370135	37.0	35.0	37.0	33.0	37.0
5	33.13493200101985	37.0	35.0	37.0	28.0	37.0
6	34.08080676572222	37.0	35.0	37.0	28.0	37.0
7	35.32871960117391	37.0	35.0	37.0	32.0	37.0
8	35.20253768830591	37.0	35.0	37.0	32.0	37.0
9	37.27951188286925	39.0	37.0	39.0	34.0	39.0
10	36.751029342360084	39.0	37.0	39.0	32.0	39.0
11	36.587270330528746	39.0	35.0	39.0	32.0	39.0
12	36.988277160262776	39.0	37.0	39.0	33.0	39.0
13	36.96712342886281	39.0	37.0	39.0	33.0	39.0
14	37.928521056086275	40.0	37.0	41.0	33.0	41.0
15	38.0517302173689	40.0	37.0	41.0	33.0	41.0
16	38.07903016691892	40.0	37.0	41.0	33.0	41.0
17	37.986638892053314	40.0	37.0	41.0	33.0	41.0
18	37.883433419586524	39.0	37.0	40.0	33.0	41.0
19	37.6769221171633	39.0	37.0	40.0	33.0	41.0
20	37.4012319559946	39.0	35.0	40.0	33.0	41.0
21	37.41356779012808	39.0	35.0	40.0	33.0	41.0
22	37.400366711691916	39.0	35.0	40.0	33.0	41.0
23	37.094238395148125	39.0	35.0	40.0	32.0	41.0
24	36.82240792878415	39.0	35.0	40.0	32.0	41.0
25	36.61814246423747	38.0	35.0	40.0	31.0	41.0
26	36.86850727727418	39.0	35.0	40.0	32.0	41.0
27	36.766408449558156	39.0	35.0	40.0	32.0	41.0
28	36.85111288318931	39.0	35.0	40.0	32.0	41.0
29	36.88456176325397	39.0	35.0	40.0	32.0	41.0
30	36.57413163647805	38.0	35.0	40.0	31.0	41.0
31	36.436487270873	38.0	35.0	40.0	31.0	41.0
32	36.15276579816753	38.0	35.0	40.0	30.0	41.0
33	35.980324507298974	39.0	35.0	40.0	30.0	41.0
34	35.8112763845265	39.0	35.0	40.0	29.0	41.0
35	35.56279937724109	39.0	35.0	40.0	26.0	41.0
36	35.34449200123684	38.0	35.0	40.0	25.0	41.0
37	35.29527614583842	38.0	35.0	40.0	25.0	41.0
38	35.24482073982456	38.0	35.0	40.0	24.0	41.0
39	35.16601298680163	38.0	35.0	40.0	23.0	41.0
40	35.0743052278115	38.0	35.0	40.0	23.0	41.0
41	34.951850103883565	38.0	35.0	40.0	22.0	41.0
42	35.036210067212394	39.0	35.0	40.0	23.0	41.0
43	34.929882663108046	38.0	35.0	40.0	22.0	41.0
44	34.84302732436083	38.0	35.0	40.0	21.0	41.0
45	34.81647869980091	38.0	35.0	40.0	22.0	41.0
46	34.622731785115626	38.0	34.0	40.0	20.0	41.0
47	34.501961039595095	38.0	34.0	40.0	20.0	41.0
48	34.48157219500816	38.0	34.0	40.0	20.0	41.0
49	34.54244308102918	38.0	34.0	40.0	20.0	41.0
50	34.468042920457194	38.0	34.0	40.0	20.0	41.0
51	33.4961511546536	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	6.0
10	10.0
11	5.0
12	9.0
13	18.0
14	18.0
15	28.0
16	53.0
17	94.0
18	181.0
19	333.0
20	636.0
21	1124.0
22	1746.0
23	2503.0
24	3514.0
25	4903.0
26	6070.0
27	6716.0
28	6406.0
29	6689.0
30	7021.0
31	8434.0
32	10805.0
33	14445.0
34	21518.0
35	31925.0
36	40618.0
37	53535.0
38	78379.0
39	60927.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.03368756814816	22.852756576127938	18.548233979418576	18.565321876305326
2	35.71072089225946	28.37024861533788	21.479486386642147	14.439544105760522
3	24.401787990734565	28.78361297812207	34.14595776305868	12.668641268084691
4	22.56416098426286	25.457440287293657	37.80520882494942	14.173189903494068
5	21.176244026017002	32.99754259768581	30.50487954388877	15.321333832408419
6	20.048442831491638	35.63721581200058	32.044417682447204	12.269923674060571
7	62.157631780233366	8.867804774846617	23.635273758957585	5.339289685962428
8	59.84371355260089	11.160837795173077	21.763742195170906	7.2317064570551315
9	54.53181874894896	10.225072013279737	23.855246526817147	11.387862710954156
10	27.970174839021162	27.29045627397052	31.204127133952834	13.53524175305548
11	18.482865992915304	24.833053959781058	37.27141547458243	19.41266457272121
12	16.711149445863917	21.71763199722254	42.42897673333659	19.14224182357696
13	18.709619672237864	23.964283583142112	43.83750766242996	13.488589082190073
14	15.054979630141965	29.090652649166493	38.22047184294324	17.633895877748305
15	12.494236225256454	27.486831469938867	42.64976321057171	17.36916909423297
16	12.822703576523942	27.745319814908243	37.457483685127016	21.974492923440796
17	13.101534655882304	28.18662153291997	37.151528959916675	21.560314851281053
18	12.764658974400703	26.452335617144314	41.68226276303156	19.100742645423427
19	13.141406415284717	27.8597816004036	39.06211857373021	19.936693410581476
20	14.090734020104046	29.73049945481472	40.078712820262446	16.100053704818787
21	14.159085607651036	29.749486006911102	39.53895226780803	16.552476117629826
22	11.617057518403394	27.103845590508897	37.53722720393184	23.74186968715587
23	12.185840371919433	28.018726165096208	38.06288346054323	21.73255000244113
24	15.605860877395697	26.10271182211228	36.08041618522195	22.21101111527007
25	11.631433050704944	31.900933595890223	34.000575021292065	22.467058332112767
26	11.031729240917647	31.496791272695713	37.16726067451083	20.304218811875817
27	14.611508020462077	31.48621305081344	35.57401771716547	18.328261211559013
28	10.538350122870115	30.62313863980341	37.16671820159378	21.67179303573269
29	12.908143060957682	29.62932825578683	36.15744733944158	21.30508134381391
30	14.307451950461372	34.23546579437022	32.27361248989644	19.18346976527197
31	14.798932413299266	35.476372592098336	29.04752605226184	20.677168942340554
32	13.85855561161109	33.410093251094445	30.34973228961544	22.38161884767903
33	14.404283366152946	29.05159459913964	28.961815331369582	27.582306703337835
34	16.509349520725177	29.587829077633298	30.00987300709012	23.892948394551404
35	13.083633049619998	31.246440021481924	28.627652014473178	27.042274914424898
36	14.830395842487565	35.00930341052723	26.334890230605236	23.825410516379968
37	14.502199727678597	32.978827282047945	32.62025268388476	19.898720306388704
38	13.687405406285091	30.655144541908747	29.357820560808502	26.29962949099766
39	19.12352650793909	25.19352721315388	31.08803792970636	24.59490834920067
40	20.21145594306204	24.743817164928043	29.355650669140342	25.689076222869573
41	17.543574137061206	28.778459485410192	25.146060832912916	28.53190554461569
42	15.652513548261105	28.25443064754992	29.709885483967213	26.383170320221762
43	17.392766666124192	27.702735690920633	30.360581747956232	24.543915894998943
44	16.946582691859106	26.778090603826605	30.119723772790643	26.155602931523642
45	18.82679382231842	24.153064158271896	29.534937968221936	27.485204051187743
46	21.355531325098596	26.179742976331905	29.777423362138645	22.687302336430854
47	14.358715641121616	26.639488773522984	35.28704954405151	23.71474604130389
48	15.864077985906553	25.232314026722214	32.20363348359833	26.6999745037729
49	17.982434726946256	21.404353887632162	35.79154935689836	24.821662028523225
50	18.43567084913286	19.941033193917793	33.09274659462626	28.530549362323086
51	17.22785489934415	20.064717019002824	29.109639201262876	33.59778888039015
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3956.0
1	4491.5
2	5027.0
3	9272.0
4	13517.0
5	8746.5
6	3976.0
7	4222.5
8	4469.0
9	4718.5
10	4968.0
11	5218.5
12	5469.0
13	5384.5
14	5300.0
15	4943.0
16	4586.0
17	4412.5
18	4239.0
19	4283.5
20	4328.0
21	4039.0
22	3750.0
23	3597.0
24	3444.0
25	3785.5
26	4228.0
27	4329.0
28	4511.5
29	4694.0
30	5032.0
31	5370.0
32	5886.5
33	6403.0
34	6889.0
35	7375.0
36	8178.0
37	8981.0
38	9905.5
39	10830.0
40	11965.0
41	13100.0
42	16258.5
43	19417.0
44	21921.5
45	24426.0
46	34900.5
47	45375.0
48	46380.0
49	47385.0
50	45359.0
51	43333.0
52	33495.0
53	23657.0
54	18140.0
55	12623.0
56	9660.0
57	6697.0
58	5586.0
59	4475.0
60	3927.0
61	3379.0
62	2818.5
63	2258.0
64	1842.0
65	1426.0
66	1079.0
67	732.0
68	620.0
69	508.0
70	421.0
71	334.0
72	251.5
73	169.0
74	136.5
75	82.5
76	61.0
77	49.0
78	37.0
79	33.5
80	30.0
81	20.0
82	10.0
83	8.0
84	6.0
85	4.0
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	368682.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.81093673217524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.26698801275049	30.633614003581528
2	11.125738865122779	9.303551275767035
3	4.713785104865599	5.912633123658168
4	2.5834026393823786	4.320579372358047
5	1.7340952538822896	3.625207347381889
6	1.214591782298196	3.04699320990539
7	0.9501460748924417	2.7808548196556764
8	0.7885549361006396	2.6376176434518666
9	0.6214587379363421	2.3385394776162487
>10	2.851218512716241	18.387958174115155
>50	0.08487978213502459	2.4836820229313377
>100	0.05066467615811545	3.975321802321425
>500	0.00789579368697903	2.23663434275939
>1k	0.005921845265234273	6.084030564590887
>5k	6.579828072482526E-4	2.2327828199059296
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8116	2.201355097346765	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCG	3056	0.8288986172365345	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGC	3037	0.8237451245246582	No Hit
GCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGC	2860	0.775736271366652	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCC	2661	0.7217602161212102	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2649	0.7185053786189725	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTC	2554	0.6927379150595906	No Hit
CCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGC	2552	0.692195442142551	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGCT	1617	0.4385893534265302	TruSeq Adapter, Index 14 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1129	0.3062259616688637	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAATCGCCT	873	0.23678942828779273	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	809	0.21943029494252497	No Hit
TCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGC	804	0.21807411264992593	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCG	799	0.2167179303573269	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	756	0.20505476264097516	No Hit
ACTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTGC	712	0.19312035846610356	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCT	671	0.18199966366679143	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGC	610	0.16545423969708312	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAATCGCCTCGTA	548	0.148637579268855	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	537	0.1456539782251371	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	507	0.13751688446954285	No Hit
CGCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTG	504	0.13670317509398344	TruSeq Adapter, Index 14 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCT	475	0.128837317796909	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	472	0.12802360842134958	No Hit
ACCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTCTTCTG	466	0.1263961896702307	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCAAATCGCCTCGTATGCCGTC	429	0.1163604407049978	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.712364585198084E-4	0.0	0.0	0.4705952555318676	0.0
2	2.712364585198084E-4	0.0	0.0	2.49130687150444	0.0
3	2.712364585198084E-4	0.0	0.0	3.3245452720772914	0.0
4	2.712364585198084E-4	0.0	0.0	4.452888939519695	0.0
5	2.712364585198084E-4	0.0	0.0	7.810253823077883	0.0
6	2.712364585198084E-4	0.0	0.0	9.270048442831492	0.0
7	2.712364585198084E-4	0.0	0.0	10.924862076260842	0.0
8	2.712364585198084E-4	0.0	0.0	13.172598608014495	0.0
9	2.712364585198084E-4	0.0	0.0	13.993360131495436	0.0
10	2.712364585198084E-4	0.0	0.0	17.28481455563331	0.0
11	2.712364585198084E-4	0.0	0.0	21.437715972030098	0.0
12	2.712364585198084E-4	0.0	0.0	25.18783124752497	0.0
13	2.712364585198084E-4	0.0	0.0	26.30369803787546	0.0
14	2.712364585198084E-4	0.0	0.0	26.79761962884003	0.0
15	2.712364585198084E-4	0.0	0.0	27.88744771917262	0.0
16	2.712364585198084E-4	0.0	0.0	29.909515517437793	0.0
17	2.712364585198084E-4	0.0	0.0	32.366917831627255	0.0
18	5.424729170396168E-4	0.0	0.0	34.85849593959021	0.0
19	5.424729170396168E-4	0.0	0.0	36.687443379389286	0.0
20	5.424729170396168E-4	0.0	0.0	38.088108451185576	0.0
21	5.424729170396168E-4	0.0	0.0	39.66046620122491	0.0
22	5.424729170396168E-4	0.0	0.0	41.29412339088971	0.0
23	5.424729170396168E-4	0.0	0.0	42.82823680027774	0.0
24	5.424729170396168E-4	0.0	0.0	43.87141221974493	0.0
25	5.424729170396168E-4	0.0	0.0	44.69190250676735	0.0
26	5.424729170396168E-4	0.0	0.0	45.39874471766997	0.0
27	5.424729170396168E-4	0.0	0.0	46.040761414986356	0.0
28	8.137093755594252E-4	0.0	0.0	46.63205689455954	0.0
29	8.137093755594252E-4	0.0	0.0	47.24722118248246	0.0
30	8.137093755594252E-4	0.0	0.0	47.86862390895134	0.0
31	8.137093755594252E-4	0.0	0.0	48.38071834263675	0.0
32	8.137093755594252E-4	0.0	0.0	48.86514665755312	0.0
33	8.137093755594252E-4	0.0	0.0	49.330317183914595	0.0
34	8.137093755594252E-4	0.0	0.0	49.74151165503062	0.0
35	8.137093755594252E-4	0.0	0.0	50.165996712614124	0.0
36	8.137093755594252E-4	0.0	0.0	50.527826148279544	0.0
37	8.137093755594252E-4	0.0	0.0	50.900505042285765	0.0
38	8.137093755594252E-4	0.0	0.0	51.324176390493705	0.0
39	8.137093755594252E-4	0.0	0.0	51.67135905739906	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTACG	20	7.027045E-4	45.000004	1
GATAAGC	20	7.027045E-4	45.000004	9
GCGCGAC	20	7.027045E-4	45.000004	9
GATCCGG	20	7.027045E-4	45.000004	5
GGCCGAT	30	2.161476E-6	45.000004	8
ACGGGTA	30	2.161476E-6	45.000004	5
TCGGAGT	20	7.027045E-4	45.000004	2
ACGGCCA	35	1.2089185E-7	45.000004	30
CGGGCTA	20	7.027045E-4	45.000004	6
ACGTGGC	20	7.027045E-4	45.000004	26
CCATACG	20	7.027045E-4	45.000004	2
GGCGAAA	20	7.027045E-4	45.000004	32
AGGGCGT	20	7.027045E-4	45.000004	6
AGTACGG	40	6.7939254E-9	45.000004	2
GGCTAAA	20	7.027045E-4	45.000004	8
CGGACGG	20	7.027045E-4	45.000004	2
CGGCCAA	25	3.8856328E-5	45.0	31
CGGGCGA	25	3.8856328E-5	45.0	6
TTCGCAG	25	3.8856328E-5	45.0	1
GCCAAAT	25	3.8856328E-5	45.0	33
>>END_MODULE