FastQCFastQC Report
Sat 14 Jan 2017
SRR2935246.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935246.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5243764
Sequences flagged as poor quality0
Sequence length51
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57198110.907832617943905No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT384500.7332519159901171No Hit
GAATCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTC153380.2924998150183723No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCC120650.23008281837245156No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA98390.1876323953557025No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCG94860.1809005897290572No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGT94620.18044290322752893No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTT83510.15925583226094842No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA74160.14142512897224208No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCG69430.13240489083795534No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCT69150.131870923252839No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTC62740.11964687960785421No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGC61310.11691983086958148No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA57670.10997825226306905No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC54700.10431438180665645No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTC53270.10158733306838372No Hit
GAACTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCT53120.10130127900492852No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1928150.044.578741
CGTTATT145850.044.2440871
GTTATTT157850.041.336712
GTTTTTT2148150.040.114872
CGACGGT1800.037.50000428
TACGGGA16950.036.3716854
CCGATGA17900.036.20111518
ACTAACG1000.036.01
CTAGCGT250.002107791636.016
ACCACCG23200.035.8836214
TATAGCG1400.035.3571431
CGAATAT2950.035.08474714
CGATGAA19050.035.07874319
CTAACGG2900.034.9137952
GCTAACG1100.034.7727281
TATGGGA32050.034.7503934
TCGATCA4450.034.38202317
CGACCAC31250.034.34412
ACGGGAT24000.034.1255
CGTTAGG4500.034.02
Produced by FastQC (version 0.11.5)