##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935246.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5243764
Sequences flagged as poor quality	0
Sequence length	51
%GC	31
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1622504368999	33.0	31.0	34.0	30.0	34.0
2	32.34071022265685	34.0	31.0	34.0	30.0	34.0
3	32.18826762607928	34.0	31.0	34.0	30.0	34.0
4	35.89211871472477	37.0	35.0	37.0	35.0	37.0
5	33.3196513039107	37.0	35.0	37.0	30.0	37.0
6	34.40806584735697	37.0	35.0	37.0	28.0	37.0
7	35.69288186882552	37.0	35.0	37.0	32.0	37.0
8	35.69318699315988	37.0	35.0	37.0	35.0	37.0
9	37.83949010672487	39.0	38.0	39.0	35.0	39.0
10	37.50390254023637	39.0	37.0	39.0	35.0	39.0
11	37.28999093017916	39.0	37.0	39.0	34.0	39.0
12	37.51169961119532	39.0	37.0	39.0	35.0	39.0
13	37.52018988650138	39.0	37.0	39.0	35.0	39.0
14	38.75324442518771	40.0	38.0	41.0	35.0	41.0
15	38.85163672507001	40.0	38.0	41.0	35.0	41.0
16	38.81146539012816	40.0	38.0	41.0	35.0	41.0
17	38.69162628218967	40.0	38.0	41.0	34.0	41.0
18	38.14765691209597	39.0	38.0	40.0	34.0	41.0
19	37.45577184633023	37.0	37.0	40.0	34.0	41.0
20	36.7217988833975	37.0	35.0	40.0	34.0	41.0
21	36.58917716357944	36.0	35.0	40.0	33.0	41.0
22	36.547385999827604	35.0	35.0	40.0	33.0	41.0
23	36.36210401535996	35.0	35.0	40.0	33.0	41.0
24	36.043865818522725	35.0	35.0	40.0	33.0	41.0
25	35.831777707768694	35.0	35.0	40.0	32.0	41.0
26	36.03995469666446	35.0	35.0	40.0	33.0	41.0
27	35.95346911111942	35.0	35.0	40.0	33.0	41.0
28	35.90805249816734	35.0	35.0	40.0	33.0	41.0
29	35.85339157139795	35.0	35.0	40.0	33.0	41.0
30	35.434436027250655	35.0	35.0	40.0	31.0	41.0
31	34.99841869313722	35.0	35.0	40.0	29.0	41.0
32	34.17067053360906	35.0	34.0	40.0	21.0	41.0
33	33.1497857264362	35.0	33.0	40.0	15.0	41.0
34	32.14441496604348	35.0	32.0	40.0	10.0	41.0
35	31.420529604307134	35.0	30.0	40.0	8.0	41.0
36	30.97384664908642	35.0	27.0	40.0	7.0	41.0
37	30.780250026507677	35.0	25.0	40.0	7.0	41.0
38	30.60059548827903	35.0	24.0	40.0	7.0	41.0
39	30.359460303705507	35.0	23.0	40.0	7.0	41.0
40	30.183756172093176	35.0	23.0	40.0	7.0	41.0
41	29.977966018302883	35.0	22.0	40.0	7.0	41.0
42	29.89644594989401	35.0	21.0	40.0	7.0	41.0
43	29.76174328211567	35.0	21.0	40.0	7.0	41.0
44	29.624798522587973	35.0	21.0	40.0	7.0	41.0
45	29.599329412994177	35.0	20.0	40.0	7.0	41.0
46	29.51016598000978	35.0	20.0	40.0	7.0	41.0
47	29.439536943310188	35.0	20.0	40.0	7.0	41.0
48	29.349123644771197	35.0	20.0	39.0	7.0	41.0
49	29.299077723558877	35.0	20.0	39.0	7.0	41.0
50	29.18738829588822	35.0	20.0	39.0	7.0	41.0
51	28.575458964209677	35.0	20.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	11.0
8	33.0
9	60.0
10	93.0
11	113.0
12	171.0
13	265.0
14	373.0
15	622.0
16	1224.0
17	2267.0
18	4586.0
19	8665.0
20	15097.0
21	23398.0
22	34997.0
23	54282.0
24	92593.0
25	168545.0
26	258096.0
27	286420.0
28	251346.0
29	201248.0
30	168246.0
31	156265.0
32	163146.0
33	191835.0
34	273635.0
35	418482.0
36	519749.0
37	458872.0
38	672437.0
39	816325.0
40	266.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.616026960786183	12.768976635866908	15.081475825380394	46.53352057796651
2	58.67453226346571	15.90411010106481	15.708697035183125	9.712660600286359
3	18.76566145997417	15.947323334917437	55.766392232754946	9.520622972353447
4	16.346578526417282	16.150402649699718	56.62733868267146	10.875680141211543
5	14.998157811831348	24.29703167419434	50.55359089386936	10.151219620104948
6	15.86644631604321	21.680056539539155	53.77385404835153	8.679643096066108
7	48.6813479782843	4.0840510747623275	44.73630010809029	2.498300838863076
8	50.011880778768834	4.323135060998169	43.38271135009127	2.2822728101417225
9	46.12856337546846	5.391070231230849	44.369769501449724	4.110596891850968
10	23.37033093022493	21.143667030018896	47.79435153832247	7.691650501433703
11	16.2609720803606	16.75437719927899	55.66081921306909	11.323831507291327
12	14.5950885661521	15.622518480999526	57.46713620216318	12.315256750685196
13	14.790825826639031	15.5958773125564	58.076545778948095	11.536751081856469
14	12.151099858803715	18.169009894419354	57.43460232001287	12.245287926764057
15	11.713284579550109	18.133825244614364	57.94580000167818	12.207090174157342
16	14.516557190598203	18.703950063351442	55.436304913798566	11.343187832251795
17	14.485529859848764	18.887749334256842	55.3792275930038	11.247493212890587
18	14.26441006879791	18.381452712212067	55.58123897261586	11.772898246374169
19	13.5260091796656	19.662784213782313	54.65184932044996	12.159357286102122
20	14.130632118455368	20.241032967921516	54.857636613699626	10.77069829992349
21	13.900606510895608	19.825968521848047	55.692876338446965	10.580548628809382
22	13.818280151433207	18.122154238825395	56.021590597898765	12.037975011842638
23	13.027436017334113	19.342670646505066	55.7794553683194	11.85043796784142
24	12.794568939410697	19.818206921592964	55.22750451774717	12.159719621249163
25	13.392135877968576	20.758676401150016	54.0580392252588	11.79114849562261
26	13.006382438263811	20.16925246826516	54.240312874492446	12.58405221897858
27	13.1870923252839	19.740209513624183	54.73470964749748	12.337988513594434
28	12.395084904660088	20.466596132091375	54.39859612293765	12.739722840310892
29	13.289938296231485	19.91319594093098	54.27500169725411	12.521864065583424
30	13.585184230259028	20.257967368478063	54.24862751260354	11.908220888659368
31	13.945955615088703	20.912973962977738	52.56169041932475	12.579380002608815
32	14.82063266005106	21.44520615344245	51.10212816595102	12.632033020555463
33	15.326738579386866	22.259964407246397	48.896041088042864	13.517255925323871
34	14.740213327678362	24.258166462106228	47.40192350380376	13.599696706411654
35	15.423501133918307	25.788231506986204	44.870383182767185	13.917884176328302
36	16.46715984929909	26.647633264960056	43.07690048598678	13.80830639975407
37	16.375756040889712	26.629325804898922	42.522356078572564	14.472562075638798
38	17.050176171162544	26.717125332108765	41.65519272034363	14.577505776385054
39	17.20065967881087	26.360644758230922	41.04341842996748	15.395277132990728
40	17.634088795758164	26.414403851889595	41.37348667865297	14.578020673699275
41	16.719097198119517	27.173877390363106	40.65859943353667	15.448425977980703
42	16.84297767786651	26.99156560058767	40.876153083929786	15.289303637616033
43	17.239410469273597	26.219162418445986	41.0171395966714	15.524287515609018
44	16.979044060716692	26.048254650666962	40.61391778882497	16.35878349979137
45	16.507417191162684	25.856674709235577	40.78049660511037	16.855411494491364
46	16.51248988322129	26.170476016845917	41.0643385171415	16.252695582791294
47	15.797602638104996	26.456091464070465	41.81854866084744	15.927757236977103
48	15.654518395564713	26.27736488522367	41.64910930392748	16.419007415284135
49	16.133067773454336	25.723144672414698	41.848927602386375	16.294859951744588
50	15.592406523253144	25.582329792111164	41.86765460840724	16.95760907622845
51	15.486738152212801	25.399464964479712	41.325048190574556	17.78874869273293
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	12319.0
1	13978.5
2	15638.0
3	408332.5
4	801027.0
5	491775.5
6	182524.0
7	175040.5
8	167557.0
9	167622.0
10	167687.0
11	166679.0
12	165671.0
13	162043.5
14	158416.0
15	150737.5
16	143059.0
17	133000.0
18	122941.0
19	112856.5
20	102772.0
21	95575.0
22	88378.0
23	83697.5
24	79017.0
25	77068.0
26	76704.5
27	78290.0
28	78835.0
29	79380.0
30	84604.5
31	89829.0
32	97205.0
33	104581.0
34	112727.5
35	120874.0
36	126399.5
37	131925.0
38	136724.5
39	141524.0
40	147620.5
41	153717.0
42	165931.0
43	178145.0
44	186621.5
45	195098.0
46	227889.5
47	260681.0
48	246216.0
49	231751.0
50	228276.5
51	224802.0
52	203560.0
53	182318.0
54	169504.5
55	156691.0
56	147638.5
57	138586.0
58	131615.0
59	124644.0
60	114158.0
61	103672.0
62	91036.5
63	78401.0
64	70823.0
65	63245.0
66	53684.5
67	44124.0
68	37010.5
69	29897.0
70	24820.0
71	19743.0
72	16344.0
73	12945.0
74	10750.5
75	6305.5
76	4055.0
77	2947.0
78	1839.0
79	1507.5
80	1176.0
81	911.0
82	646.0
83	452.0
84	258.0
85	215.5
86	173.0
87	101.0
88	29.0
89	23.0
90	17.0
91	15.0
92	13.0
93	10.5
94	8.0
95	4.5
96	1.0
97	1.5
98	2.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5243764.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.26804917049331
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.64674466495019	21.718113497935875
2	7.1264028178263095	3.886462048904597
3	2.899031601329635	2.371528087556113
4	1.6382280875429427	1.786851361744167
5	1.174733784698128	1.6016349301694128
6	0.8803443409456299	1.4403163665522576
7	0.6934229865105471	1.323580446448394
8	0.573297790655921	1.2506169875952666
9	0.473265201941819	1.161451691750967
>10	3.940040977139546	22.397350117051815
>50	0.7229563494977698	13.671683746027735
>100	0.21764629432606378	8.985352184914799
>500	0.008505534082128846	1.6369650799835564
>1k	0.0040710248510895854	2.185725709436148
>5k	0.0010177562127723964	1.9385723098492562
>10k+	2.9078748936354186E-4	12.643795434079703
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	571981	10.907832617943905	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38450	0.7332519159901171	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTC	15338	0.2924998150183723	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCC	12065	0.23008281837245156	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9839	0.1876323953557025	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCG	9486	0.1809005897290572	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGT	9462	0.18044290322752893	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTT	8351	0.15925583226094842	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	7416	0.14142512897224208	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCG	6943	0.13240489083795534	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCT	6915	0.131870923252839	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTC	6274	0.11964687960785421	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGC	6131	0.11691983086958148	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	5767	0.10997825226306905	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	5470	0.10431438180665645	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTC	5327	0.10158733306838372	No Hit
GAACTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCT	5312	0.10130127900492852	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.721081269103644E-5	0.0	0.0	0.041420628388310385	0.0
2	5.721081269103644E-5	0.0	0.0	0.18727006020865927	0.0
3	5.721081269103644E-5	0.0	0.0	0.3241755349783095	0.0
4	7.628108358804859E-5	0.0	0.0	0.568332213272756	0.0
5	7.628108358804859E-5	0.0	0.0	1.1839968389118962	0.0
6	7.628108358804859E-5	0.0	0.0	1.7101074724186671	0.0
7	7.628108358804859E-5	0.0	0.0	2.14283098934277	0.0
8	7.628108358804859E-5	0.0	0.0	2.634443502796846	0.0
9	7.628108358804859E-5	0.0	0.0	3.04111702967563	0.0
10	7.628108358804859E-5	0.0	0.0	3.6715229747181604	0.0
11	7.628108358804859E-5	0.0	0.0	4.227154387573506	0.0
12	7.628108358804859E-5	0.0	0.0	4.702671592390504	0.0
13	7.628108358804859E-5	0.0	0.0	4.8916198364381005	0.0
14	7.628108358804859E-5	0.0	0.0	5.030470478839246	0.0
15	7.628108358804859E-5	0.0	0.0	5.152272299058462	0.0
16	7.628108358804859E-5	0.0	0.0	5.283819027706052	0.0
17	7.628108358804859E-5	0.0	0.0	5.431689908241484	0.0
18	9.535135448506073E-5	0.0	0.0	5.580361740154591	0.0
19	9.535135448506073E-5	0.0	0.0	5.742478113050091	0.0
20	9.535135448506073E-5	0.0	0.0	5.859397943919673	0.0
21	9.535135448506073E-5	0.0	0.0	5.977290358605002	0.0
22	1.1442162538207288E-4	0.0	0.0	6.122491401214853	0.0
23	1.1442162538207288E-4	0.0	0.0	6.250128724328555	0.0
24	1.1442162538207288E-4	0.0	0.0	6.349961592474413	0.0
25	1.3349189627908503E-4	0.0	0.0	6.443119865806318	0.0
26	1.5256216717609718E-4	0.0	0.0	6.5420182906782225	0.0
27	1.5256216717609718E-4	0.0	0.0	6.655448261973651	0.0
28	1.7163243807310932E-4	0.0	0.0	6.754861584159776	0.0
29	1.7163243807310932E-4	0.0	0.0	6.865431014820651	0.0
30	1.9070270897012147E-4	0.0	0.0	6.999323386788574	0.0
31	2.097729798671336E-4	0.0	0.0	7.115823671698421	0.0
32	2.097729798671336E-4	0.0	0.0	7.241229773117173	0.0
33	2.097729798671336E-4	0.0	0.0	7.363813474443167	0.0
34	2.097729798671336E-4	0.0	0.0	7.48449014867946	0.0
35	2.097729798671336E-4	0.0	0.0	7.610010671723594	0.0
36	2.097729798671336E-4	0.0	0.0	7.731679000046531	0.0
37	2.097729798671336E-4	0.0	0.0	7.8678979450638895	0.0
38	2.097729798671336E-4	0.0	0.0	8.04111321562145	0.0
39	2.097729798671336E-4	0.0	0.0	8.30828771088859	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	192815	0.0	44.57874	1
CGTTATT	14585	0.0	44.244087	1
GTTATTT	15785	0.0	41.33671	2
GTTTTTT	214815	0.0	40.11487	2
CGACGGT	180	0.0	37.500004	28
TACGGGA	1695	0.0	36.371685	4
CCGATGA	1790	0.0	36.201115	18
ACTAACG	100	0.0	36.0	1
CTAGCGT	25	0.0021077916	36.0	16
ACCACCG	2320	0.0	35.88362	14
TATAGCG	140	0.0	35.357143	1
CGAATAT	295	0.0	35.084747	14
CGATGAA	1905	0.0	35.078743	19
CTAACGG	290	0.0	34.913795	2
GCTAACG	110	0.0	34.772728	1
TATGGGA	3205	0.0	34.750393	4
TCGATCA	445	0.0	34.382023	17
CGACCAC	3125	0.0	34.344	12
ACGGGAT	2400	0.0	34.125	5
CGTTAGG	450	0.0	34.0	2
>>END_MODULE