##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935245.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1597899
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05921588285617	33.0	31.0	34.0	30.0	34.0
2	32.19810013023351	34.0	31.0	34.0	30.0	34.0
3	32.110848683177096	33.0	31.0	34.0	30.0	34.0
4	35.8428279885024	37.0	35.0	37.0	35.0	37.0
5	33.27930363558648	37.0	35.0	37.0	30.0	37.0
6	34.35982436937503	37.0	35.0	37.0	28.0	37.0
7	35.63270769929764	37.0	35.0	37.0	32.0	37.0
8	35.61383041105852	37.0	35.0	37.0	33.0	37.0
9	37.744017613128236	39.0	38.0	39.0	35.0	39.0
10	37.30197090053877	39.0	37.0	39.0	34.0	39.0
11	37.08691162582867	39.0	37.0	39.0	34.0	39.0
12	37.3566458205431	39.0	37.0	39.0	34.0	39.0
13	37.37121620327693	39.0	37.0	39.0	34.0	39.0
14	38.523725216675146	40.0	38.0	41.0	34.0	41.0
15	38.62747207426752	40.0	38.0	41.0	34.0	41.0
16	38.604240943889444	40.0	38.0	41.0	34.0	41.0
17	38.51442425334768	40.0	38.0	41.0	34.0	41.0
18	38.16859826559751	39.0	38.0	41.0	34.0	41.0
19	37.68735821225246	39.0	37.0	41.0	34.0	41.0
20	37.19111283003494	39.0	35.0	40.0	33.0	41.0
21	37.10484016824593	38.0	35.0	40.0	33.0	41.0
22	37.074924635411875	38.0	35.0	40.0	33.0	41.0
23	36.85400391388943	38.0	35.0	40.0	33.0	41.0
24	36.561044847014735	38.0	35.0	40.0	33.0	41.0
25	36.35679163701836	38.0	35.0	40.0	32.0	41.0
26	36.57263068566912	38.0	35.0	40.0	33.0	41.0
27	36.46858468526484	38.0	35.0	40.0	33.0	41.0
28	36.4570113630461	38.0	35.0	40.0	33.0	41.0
29	36.42350987139988	38.0	35.0	40.0	33.0	41.0
30	36.07330688610482	38.0	35.0	40.0	31.0	41.0
31	35.749449746197975	38.0	35.0	40.0	30.0	41.0
32	35.13811010583272	38.0	35.0	40.0	25.0	41.0
33	34.44576033904521	38.0	34.0	40.0	21.0	41.0
34	33.792931843627166	38.0	34.0	40.0	15.0	41.0
35	33.27483651970494	38.0	33.0	40.0	12.0	41.0
36	32.975066634374265	37.0	33.0	40.0	10.0	41.0
37	32.8584841720284	37.0	33.0	40.0	10.0	41.0
38	32.70528362556082	37.0	33.0	40.0	10.0	41.0
39	32.529954646695444	37.0	33.0	40.0	9.0	41.0
40	32.38543737745627	37.0	32.0	40.0	9.0	41.0
41	32.26439405744669	37.0	31.0	40.0	8.0	41.0
42	32.232457120256036	37.0	31.0	40.0	8.0	41.0
43	32.140508254902215	37.0	31.0	40.0	8.0	41.0
44	32.04403657552824	36.0	31.0	40.0	8.0	41.0
45	32.019087564357946	36.0	31.0	40.0	8.0	41.0
46	31.912381195557415	36.0	31.0	40.0	8.0	41.0
47	31.815654806718072	36.0	31.0	40.0	8.0	41.0
48	31.733173373285798	35.0	31.0	40.0	8.0	41.0
49	31.696622877916564	35.0	30.0	40.0	8.0	41.0
50	31.581573678937154	35.0	30.0	40.0	7.0	41.0
51	30.805126606875653	35.0	27.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	18.0
10	24.0
11	33.0
12	41.0
13	69.0
14	96.0
15	164.0
16	273.0
17	577.0
18	1094.0
19	2035.0
20	3620.0
21	5996.0
22	9368.0
23	15020.0
24	24216.0
25	40899.0
26	58034.0
27	60418.0
28	51912.0
29	43395.0
30	39178.0
31	39634.0
32	45009.0
33	56828.0
34	84423.0
35	121838.0
36	158551.0
37	173221.0
38	262748.0
39	299069.0
40	92.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.650059859853467	16.346840444859158	19.236322195583075	33.7667774997043
2	48.69788390880775	20.32080876200561	19.474134472829636	11.507172856357004
3	22.310734282955305	20.25102963328721	46.46094653041275	10.977289553344736
4	19.544852334221375	20.052957039212117	47.57428348099598	12.827907145570528
5	17.644732239021366	28.368000730959842	41.8930107597539	12.094256270264891
6	18.749870924257415	26.87723066351503	43.97255395991862	10.400344452308937
7	57.91899237686488	5.583957434105661	33.12875219272307	3.3682979963063997
8	58.7232359492058	5.939611952945712	31.889625063912046	3.447527033936438
9	53.9919606933855	7.279746717408296	33.35692681452332	5.3713657746828805
10	26.339462006046688	26.208289760491745	38.13013212975288	9.322116103708684
11	19.014969031209105	20.22042694813627	46.575909991808	14.18869402884663
12	17.37343849642562	18.799623755944523	49.00578822566383	14.821149521966031
13	17.460990963759286	18.998697664871184	50.38703948121878	13.153271890150755
14	14.752809783346757	21.942876239361812	48.59161937018548	14.712694607105956
15	14.13418495161459	21.441968484866692	49.53767415837922	14.8861724051395
16	16.395967454764037	22.40135327702189	46.345795322482836	14.856883945731239
17	16.310793110202834	21.944941451243164	46.64468780567483	15.099577632879175
18	15.798808310162283	22.158722172052176	47.51896083544705	14.523508682338496
19	15.546226638855146	22.946256302807626	46.311249960103865	15.196267098233369
20	16.147078131972044	23.840743376145802	46.57841327893691	13.433765212945248
21	15.929980555717227	23.06372305133178	47.686993983975206	13.319302408975787
22	15.250776175465408	21.374129403673198	47.49999843544555	15.875095985415848
23	14.399283058566281	23.09150953846269	47.146221381952174	15.362986021018852
24	14.807256278400574	23.559373902856187	46.35292969080023	15.280440127943004
25	14.832664642759022	24.996698790098748	44.76459400750611	15.406042559636122
26	14.426944381340748	24.424760263320774	45.386035037258296	15.762260318080179
27	14.626644112049636	23.778849601883472	46.044399552161934	15.55010673390496
28	13.31485907432197	24.46349863164067	45.604196510542906	16.61744578349445
29	14.585339874422601	24.122988999930534	45.13307787288183	16.158593252765037
30	15.400723074487185	24.367372405890485	44.50331341342601	15.728591106196324
31	15.355288413097448	25.881986283238177	42.85546207864201	15.907263225022358
32	16.387393696347516	26.121363114940305	41.91929527460747	15.571947914104708
33	16.70487308647167	25.39378271092228	40.99771011809883	16.903634084507217
34	15.879038662643886	26.527458869427917	39.70551330215489	17.887989165773305
35	16.40260116565565	27.905643598249952	37.74662854160369	17.945126694490703
36	17.79618111032049	28.854326837929058	36.006593658297554	17.342898393452902
37	17.44165307068845	28.807202457727303	36.08626077117515	17.6648837004091
38	18.927541728231883	28.26323816461491	36.203039115738854	16.60618099141435
39	19.373502330247405	26.334267685254197	36.14120792365475	18.151022060843644
40	18.58847148662087	26.867467843712273	36.85627189202822	17.687788777638637
41	18.061154052915736	27.83423733289776	34.83505528196713	19.26955333221937
42	18.463557458888204	28.038505562616912	35.54173323845875	17.956203740036138
43	19.086062385670182	26.767273776377603	35.85839906026601	18.2882647776862
44	18.47613647671098	26.388088358525792	35.37926990379241	19.756505260970812
45	18.03562052420084	25.869031772346062	35.802450592934846	20.292897110518247
46	18.572137538104723	26.62120697240564	36.081379361273775	18.725276128215864
47	16.43971239734176	27.12205214472254	37.59161248614587	18.84662297178983
48	17.092006440957782	27.079183352639934	36.517952636555876	19.310857569846405
49	18.12968153806968	25.909397277299757	37.36919542474212	18.59172575988845
50	17.286386686517734	25.476954425780352	37.08075416531333	20.155904722388588
51	16.823591478560285	25.03869143168623	35.96097125037315	22.176745839380335
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6110.0
1	8057.5
2	10005.0
3	82332.0
4	154659.0
5	95079.5
6	35500.0
7	34519.0
8	33538.0
9	34403.5
10	35269.0
11	35966.5
12	36664.0
13	35687.5
14	34711.0
15	33438.5
16	32166.0
17	29989.5
18	27813.0
19	27218.5
20	26624.0
21	24679.0
22	22734.0
23	22336.5
24	21939.0
25	21913.5
26	22882.0
27	23876.0
28	25776.0
29	27676.0
30	29297.0
31	30918.0
32	33276.0
33	35634.0
34	38431.0
35	41228.0
36	44106.0
37	46984.0
38	48188.0
39	49392.0
40	52482.5
41	55573.0
42	62310.0
43	69047.0
44	73632.0
45	78217.0
46	93394.0
47	108571.0
48	109262.5
49	109954.0
50	105827.5
51	101701.0
52	87033.5
53	72366.0
54	64199.0
55	56032.0
56	50520.0
57	45008.0
58	41439.0
59	37870.0
60	33895.5
61	29921.0
62	27395.0
63	24869.0
64	20974.0
65	17079.0
66	14243.0
67	11407.0
68	9714.0
69	8021.0
70	6805.0
71	5589.0
72	5226.0
73	4863.0
74	3986.0
75	2468.0
76	1827.0
77	1342.0
78	857.0
79	589.5
80	322.0
81	257.5
82	193.0
83	136.5
84	80.0
85	75.0
86	70.0
87	39.0
88	8.0
89	7.5
90	7.0
91	5.0
92	3.0
93	4.0
94	5.0
95	3.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1597899.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.24601881712005
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.06805060391383	26.735199299975132
2	8.601496329800813	5.891340103314954
3	3.3074029463946144	3.397961506140848
4	1.9429333451348711	2.6615092759159524
5	1.3045145852723519	2.233721551722226
6	0.9458280018471474	1.9434506129409883
7	0.7238237199549497	1.7351656513698528
8	0.6114965448417127	1.675305774500255
9	0.4709841794192121	1.4516399763860564
>10	3.440374947085309	23.837166333237743
>50	0.4517029482868893	10.55554228433679
>100	0.12284758405209509	6.255289980028603
>500	0.003987323198265084	1.0269856360554261
>1k	0.003987323198265084	2.6378848476317924
>5k	3.7974506650143664E-4	0.7849020902130587
>10k+	1.8987253325071832E-4	7.176935076230316
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	110374	6.9074453391609865	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCC	6668	0.4172979643894889	No Hit
GAATCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTC	5403	0.3381315089376738	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4501	0.28168238418072733	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCG	3171	0.19844808714443155	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGC	2827	0.17691981783579563	No Hit
GCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGC	2824	0.1767320713011273	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCG	2645	0.16552986139924988	No Hit
CCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGC	2360	0.14769394060575794	No Hit
CTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGCT	1976	0.12366238416821089	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGT	1951	0.12209782971264141	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1872	0.11715383763304188	No Hit
GAACTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCT	1705	0.10670261386983783	No Hit
CGCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTG	1662	0.10401158020625834	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTC	1615	0.10107021782978774	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGC	1608	0.1006321425822283	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13211097822828602	0.0
2	0.0	0.0	0.0	0.58645759212566	0.0
3	0.0	0.0	0.0	0.8576261703649605	0.0
4	0.0	0.0	0.0	1.262032206040557	0.0
5	0.0	0.0	0.0	2.3375069387990104	0.0
6	0.0	0.0	0.0	3.0190268596450713	0.0
7	0.0	0.0	0.0	3.6960408636590922	0.0
8	0.0	0.0	0.0	4.5639930934308115	0.0
9	6.258217822277878E-5	0.0	0.0	5.0107672637632295	0.0
10	6.258217822277878E-5	0.0	0.0	6.049068182657352	0.0
11	6.258217822277878E-5	0.0	0.0	7.427127747122941	0.0
12	6.258217822277878E-5	0.0	0.0	8.560678741272133	0.0
13	6.258217822277878E-5	0.0	0.0	8.97140557694823	0.0
14	6.258217822277878E-5	0.0	0.0	9.194949117559997	0.0
15	6.258217822277878E-5	0.0	0.0	9.506045125505429	0.0
16	6.258217822277878E-5	0.0	0.0	9.998191375049363	0.0
17	6.258217822277878E-5	0.0	0.0	10.626954519653621	0.0
18	6.258217822277878E-5	0.0	0.0	11.256781561287665	0.0
19	1.2516435644555757E-4	0.0	0.0	11.77646396924962	0.0
20	1.2516435644555757E-4	0.0	0.0	12.207217101957008	0.0
21	1.2516435644555757E-4	0.0	0.0	12.666507707933981	0.0
22	1.2516435644555757E-4	0.0	0.0	13.172609783221592	0.0
23	1.2516435644555757E-4	0.0	0.0	13.626893814940743	0.0
24	1.2516435644555757E-4	0.0	0.0	13.954136024867655	0.0
25	1.2516435644555757E-4	0.0	0.0	14.214728214987305	0.0
26	1.2516435644555757E-4	0.0	0.0	14.465995660551762	0.0
27	1.2516435644555757E-4	0.0	0.0	14.72877822690921	0.0
28	2.5032871289111514E-4	0.0	0.0	14.976415906136745	0.0
29	2.5032871289111514E-4	0.0	0.0	15.229748563582554	0.0
30	2.5032871289111514E-4	0.0	0.0	15.503545593307212	0.0
31	2.5032871289111514E-4	0.0	0.0	15.7609460923375	0.0
32	2.5032871289111514E-4	0.0	0.0	15.99562926067292	0.0
33	2.5032871289111514E-4	0.0	0.0	16.218609561680683	0.0
34	2.5032871289111514E-4	0.0	0.0	16.42913600922211	0.0
35	2.5032871289111514E-4	0.0	0.0	16.671391621122485	0.0
36	2.5032871289111514E-4	0.0	0.0	16.898314599358283	0.0
37	2.5032871289111514E-4	0.0	0.0	17.13431199343638	0.0
38	2.5032871289111514E-4	0.0	0.0	17.381949672663918	0.0
39	2.5032871289111514E-4	0.0	0.0	17.756253680614357	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTA	20	7.0341135E-4	45.000004	41
CATATCG	20	7.0341135E-4	45.000004	1
TATACGA	20	7.0341135E-4	45.000004	32
TCCGTAT	25	3.891492E-5	45.0	40
CGTTTTT	38780	0.0	44.257347	1
CGTTATT	2690	0.0	44.16357	1
CGGTCTA	60	3.6379788E-12	41.249996	31
CGGGTAC	50	1.0822987E-9	40.5	6
ACGGGTC	50	1.0822987E-9	40.5	5
ATGATCG	45	1.9290383E-8	40.0	10
TCGATAG	45	1.9290383E-8	40.0	1
TAATACG	85	0.0	39.705883	4
ATATCGG	40	3.459354E-7	39.375004	2
GGCGATA	360	0.0	39.375	8
GTTATTT	3085	0.0	39.23825	2
GTTTTTT	44940	0.0	38.786716	2
TCGGGTA	70	0.0	38.571426	5
ACTACGG	70	0.0	38.571426	2
GTATCCG	35	6.24982E-6	38.571426	1
ATACGAG	35	6.24982E-6	38.571426	1
>>END_MODULE