SRR2935244.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935244.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	493842
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13253	2.683651856261719	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCG	3967	0.803293361034501	No Hit
GAATCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTC	3785	0.7664394684939717	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGC	3697	0.7486200039688807	No Hit
GCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGC	3147	0.6372483506870619	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCC	3076	0.6228712827179543	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2070	0.4191624041697547	No Hit
CCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGC	1561	0.31609300140530777	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATACCGCAT	1272	0.2575722599535884	No Hit
CTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGCT	1223	0.2476500581157536	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCT	998	0.20208892722773678	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCG	944	0.19115425581461276	No Hit
TCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGC	934	0.18912931666403424	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	895	0.181232053976778	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	851	0.17232232171423248	No Hit
GAACTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCT	802	0.1624001198763977	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	780	0.15794525374512497	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGC	691	0.1399232953049761	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	665	0.13465845351347192	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTC	630	0.1275711664864471	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATACCGCATCGTA	617	0.124938745590695	No Hit
ACTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGC	588	0.11906642205401728	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	571	0.11562402549803379	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAA	35	1.2100463E-7	45.000004	33
TTAGCGG	35	1.2100463E-7	45.000004	2
TACGGGT	70	0.0	45.000004	4
CCATCGA	60	0.0	45.000004	41
TCGTAAC	35	1.2100463E-7	45.000004	34
CAGCGTG	20	7.029374E-4	45.0	1
ACCGGGT	20	7.029374E-4	45.0	4
GCGACCG	40	6.8012014E-9	45.0	33
TTAGACG	20	7.029374E-4	45.0	1
GTTAGCG	20	7.029374E-4	45.0	1
CGTTATT	465	0.0	45.0	1
ACGATAG	20	7.029374E-4	45.0	1
TGACCGG	20	7.029374E-4	45.0	2
CTACGAA	20	7.029374E-4	45.0	11
CGAATAT	20	7.029374E-4	45.0	14
TCCGTAG	20	7.029374E-4	45.0	1
GGGCCGA	45	3.8380676E-10	45.0	7
TACACGC	20	7.029374E-4	45.0	35
TGCACGG	50	2.1827873E-11	45.0	2
AGTAACG	20	7.029374E-4	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content