##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935244.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	493842
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.69041110314635	31.0	31.0	34.0	30.0	34.0
2	31.791978001061068	33.0	31.0	34.0	30.0	34.0
3	31.78726394271852	33.0	31.0	34.0	30.0	34.0
4	35.60436333888167	37.0	35.0	37.0	33.0	37.0
5	32.97664435183723	37.0	35.0	37.0	28.0	37.0
6	34.02392060618578	37.0	35.0	37.0	27.0	37.0
7	35.36439589990321	37.0	35.0	37.0	32.0	37.0
8	35.269152886955744	37.0	35.0	37.0	33.0	37.0
9	37.33837543181827	39.0	37.0	39.0	34.0	39.0
10	36.74190125586726	39.0	37.0	39.0	32.0	39.0
11	36.60462658097124	39.0	35.0	39.0	32.0	39.0
12	37.00895833080216	39.0	37.0	39.0	33.0	39.0
13	36.98297836150024	39.0	37.0	39.0	33.0	39.0
14	38.01387893293806	40.0	37.0	41.0	33.0	41.0
15	38.11751126878637	40.0	37.0	41.0	33.0	41.0
16	38.13612045958019	40.0	37.0	41.0	33.0	41.0
17	38.009665034565714	40.0	37.0	41.0	33.0	41.0
18	37.89479631137085	39.0	37.0	40.0	33.0	41.0
19	37.61679849020537	39.0	37.0	40.0	33.0	41.0
20	37.23940248095545	39.0	35.0	40.0	33.0	41.0
21	37.251124853698144	39.0	35.0	40.0	33.0	41.0
22	37.263762498936906	39.0	35.0	40.0	33.0	41.0
23	36.927561851766356	39.0	35.0	40.0	32.0	41.0
24	36.66754954013632	38.0	35.0	40.0	31.0	41.0
25	36.51337067321127	38.0	35.0	40.0	31.0	41.0
26	36.74479286897429	38.0	35.0	40.0	32.0	41.0
27	36.623978924433324	38.0	35.0	40.0	32.0	41.0
28	36.69214850093755	38.0	35.0	40.0	32.0	41.0
29	36.69329866637508	39.0	35.0	40.0	32.0	41.0
30	36.3986902693574	38.0	35.0	40.0	31.0	41.0
31	36.198328615225115	38.0	35.0	40.0	30.0	41.0
32	35.76485799101737	38.0	35.0	40.0	28.0	41.0
33	35.41711721562767	38.0	35.0	40.0	25.0	41.0
34	35.12023278700475	38.0	35.0	40.0	23.0	41.0
35	34.840471243839126	38.0	35.0	40.0	21.0	41.0
36	34.65272496061493	38.0	35.0	40.0	20.0	41.0
37	34.60854078834931	38.0	35.0	40.0	18.0	41.0
38	34.47535446559831	38.0	34.0	40.0	18.0	41.0
39	34.34812551382831	38.0	34.0	40.0	18.0	41.0
40	34.34621397127016	38.0	34.0	40.0	18.0	41.0
41	34.19215660069415	38.0	34.0	40.0	17.0	41.0
42	34.27484094102972	38.0	34.0	40.0	17.0	41.0
43	34.159131868087364	38.0	34.0	40.0	15.0	41.0
44	34.106888438002436	38.0	34.0	40.0	15.0	41.0
45	34.104802750677344	38.0	34.0	40.0	15.0	41.0
46	33.94147115879168	38.0	33.0	40.0	15.0	41.0
47	33.81180620522353	38.0	33.0	40.0	15.0	41.0
48	33.77436508032934	38.0	33.0	40.0	15.0	41.0
49	33.77712507239158	38.0	33.0	40.0	14.0	41.0
50	33.69789932812519	38.0	33.0	40.0	13.0	41.0
51	32.73983986781197	37.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	7.0
10	9.0
11	9.0
12	14.0
13	15.0
14	35.0
15	34.0
16	55.0
17	155.0
18	254.0
19	531.0
20	1052.0
21	1789.0
22	2945.0
23	4437.0
24	6271.0
25	8948.0
26	11097.0
27	11434.0
28	10389.0
29	9871.0
30	10067.0
31	11672.0
32	14498.0
33	18921.0
34	27519.0
35	38700.0
36	50542.0
37	67754.0
38	100951.0
39	83845.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.15763341311594	20.73497191409398	22.148986922942964	19.95840774984712
2	38.11421466784923	26.675130912316085	21.91490395713609	13.295750462698596
3	23.927288484981027	26.262448313428184	36.87576188335541	12.934501318235386
4	21.660571599823424	22.265825911931348	41.430255020836626	14.643347467408605
5	20.927138639483882	29.938725341303495	34.05623661008987	15.077899409122756
6	18.952215485924647	33.72353910764981	35.946112319324804	11.378133087100732
7	60.94337865147153	6.781116227457365	27.4259783493506	4.84952677172051
8	59.186743938344655	9.736919905556839	24.159549005552382	6.916787150546126
9	54.14748036821494	8.36198622231402	26.058941928795036	11.431591480676005
10	29.42540326663184	21.289197759607323	34.646303878568446	14.639095095192388
11	20.491371734279383	22.60702815880383	37.28662203700779	19.614978069909
12	17.204085517230208	19.40640933739941	43.67368510576257	19.71582003960781
13	19.24623665058865	21.857800673089773	45.32360552565395	13.57235715066762
14	15.812749826867703	26.154721550617406	40.389233803524206	17.64329481899069
15	13.478197480165722	24.121480149521506	44.75945747830278	17.640864892009994
16	13.81656481222739	24.432308309135312	39.31662353546276	22.43450334317454
17	14.126785490096022	26.490658955698382	38.880046654598026	20.502508899607566
18	14.149262314667443	24.174533555266663	42.53283438832663	19.14336974173926
19	13.689398633571063	26.707934926555453	39.72890114652054	19.873765293352935
20	15.454538091130363	26.849478173180895	41.59265514071302	16.10332859497572
21	14.662989377169216	28.265720615095518	40.73934578265923	16.331944225076036
22	12.521211237602309	24.48050186091908	39.48185857014997	23.516428331328644
23	13.472122662713987	25.957289983436	39.15179348860567	21.41879386524435
24	15.684368684721022	24.25148124298865	38.65224100015794	21.41190907213238
25	12.585199314760592	29.615140065041047	36.253093094552504	21.546567525645855
26	12.62590059168722	27.091863389505143	40.31451354886786	19.967722469939776
27	15.801410167624464	26.07149655152863	39.359957233285144	18.76713604756177
28	11.558352671502222	27.279980236593893	39.46059670906889	21.701070382835
29	13.979167426018849	25.70255263829322	37.79974161776439	22.518538317923547
30	14.915296795331301	28.155563925304044	37.45732440740156	19.471814871963097
31	14.77395604262092	31.14012173934173	31.849255429874333	22.236666788163014
32	14.763831346868026	29.436945419790135	31.156928734291533	24.642294499050305
33	15.138647583640111	26.80918188408438	31.50785068908679	26.544319843188713
34	16.352193616581822	28.05796185824616	30.74708105021444	24.84276347495758
35	14.247269369555445	27.409778836145975	30.9149485057974	27.42800328850118
36	15.16983164655902	33.37707201898583	30.51704796270872	20.93604837174643
37	14.868925688783053	29.043702236747787	33.62532955884676	22.462042515622404
38	14.490667055454983	29.03033763835397	27.710887287837	28.76810801835405
39	19.582376549584684	25.368640172362824	28.982144086570198	26.066839191482295
40	19.736271925028653	23.49293903718193	30.461969617812983	26.30881941997643
41	17.727532287654757	28.706144880346347	28.27544032301829	25.290882508980605
42	16.23434215801815	28.180268182941106	32.48306138400541	23.102328275035337
43	16.94833570251214	27.775482846740456	32.22670408754217	23.049477363205238
44	17.424803884643268	24.29279000166045	30.95929467319507	27.323111440501215
45	18.791232823453655	22.434908331004653	30.25583081228409	28.518028033257604
46	20.56548450719056	24.919508668764504	30.884574418538723	23.630432405506212
47	14.946075870420096	25.936230616269977	36.08522563896955	23.032467874340377
48	16.303595076967937	25.1463018536293	33.38071690945687	25.169386159945894
49	17.485957046990737	22.41627889081933	35.49698081572649	24.600783246463443
50	17.90613192073578	20.983431947869967	33.433770315202025	27.676665816192227
51	16.902774571624125	20.79754253384686	30.623154774199037	31.676528120329984
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2903.0
1	3975.5
2	5048.0
3	13435.0
4	21822.0
5	14277.0
6	6732.0
7	6530.0
8	6328.0
9	6624.0
10	6920.0
11	7077.5
12	7235.0
13	7029.5
14	6824.0
15	6473.5
16	6123.0
17	5776.5
18	5430.0
19	5469.5
20	5509.0
21	5048.5
22	4588.0
23	4671.0
24	4754.0
25	4765.5
26	5147.5
27	5518.0
28	5863.5
29	6209.0
30	6643.5
31	7078.0
32	7566.5
33	8055.0
34	8799.5
35	9544.0
36	10468.5
37	11393.0
38	12159.0
39	12925.0
40	14585.0
41	16245.0
42	20404.0
43	24563.0
44	29754.0
45	34945.0
46	48931.5
47	62918.0
48	61686.5
49	60455.0
50	56934.0
51	53413.0
52	42515.0
53	31617.0
54	24852.0
55	18087.0
56	14400.5
57	10714.0
58	9238.0
59	7762.0
60	6829.0
61	5896.0
62	4902.0
63	3908.0
64	3376.5
65	2845.0
66	2287.0
67	1729.0
68	1420.5
69	1112.0
70	912.5
71	713.0
72	617.5
73	522.0
74	403.0
75	247.0
76	210.0
77	144.5
78	79.0
79	75.5
80	72.0
81	44.5
82	17.0
83	14.5
84	12.0
85	7.0
86	2.0
87	2.0
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	493842.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.67763118600122
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.9820762998972	36.703230417591044
2	10.187775724616694	9.714580272079424
3	4.1070546818315155	5.8744391514330765
4	2.2958692520120514	4.378464297948442
5	1.4593145949747448	3.4788331521777316
6	1.00290711872019	2.8689741432093814
7	0.7820334963311417	2.609985322922265
8	0.6155243162988872	2.3477393070811123
9	0.4450305207150611	1.9096200929850082
>10	2.012275039265431	15.198783431818914
>50	0.062140655737094284	2.0213918411656944
>100	0.038141505932136346	3.2871881066085136
>500	0.0055712312035704785	2.03630760010321
>1k	0.0038570062178564847	4.8625374540694555
>5k	0.0	0.0
>10k+	4.2855624642849834E-4	2.7079254088067275
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13253	2.683651856261719	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCG	3967	0.803293361034501	No Hit
GAATCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTC	3785	0.7664394684939717	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGC	3697	0.7486200039688807	No Hit
GCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGC	3147	0.6372483506870619	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCC	3076	0.6228712827179543	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2070	0.4191624041697547	No Hit
CCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGC	1561	0.31609300140530777	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATACCGCAT	1272	0.2575722599535884	No Hit
CTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGCT	1223	0.2476500581157536	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCT	998	0.20208892722773678	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCG	944	0.19115425581461276	No Hit
TCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGC	934	0.18912931666403424	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	895	0.181232053976778	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	851	0.17232232171423248	No Hit
GAACTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCT	802	0.1624001198763977	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	780	0.15794525374512497	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGC	691	0.1399232953049761	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	665	0.13465845351347192	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTC	630	0.1275711664864471	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATACCGCATCGTA	617	0.124938745590695	No Hit
ACTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGC	588	0.11906642205401728	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	571	0.11562402549803379	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3282426363087789	0.0
2	0.0	0.0	0.0	1.951028871582409	0.0
3	2.024939150578525E-4	0.0	0.0	2.6370782557984134	0.0
4	2.024939150578525E-4	0.0	0.0	3.727507988384949	0.0
5	2.024939150578525E-4	0.0	0.0	6.963968232754605	0.0
6	2.024939150578525E-4	0.0	0.0	8.544028251951028	0.0
7	2.024939150578525E-4	0.0	0.0	10.252469413294131	0.0
8	2.024939150578525E-4	0.0	0.0	12.509466590528955	0.0
9	2.024939150578525E-4	0.0	0.0	13.303647725385852	0.0
10	2.024939150578525E-4	0.0	0.0	16.559547385601064	0.0
11	2.024939150578525E-4	0.0	0.0	20.32978159006322	0.0
12	2.024939150578525E-4	0.0	0.0	23.627394996780346	0.0
13	2.024939150578525E-4	0.0	0.0	24.59734084990746	0.0
14	2.024939150578525E-4	0.0	0.0	24.987546624223942	0.0
15	2.024939150578525E-4	0.0	0.0	25.855030556331783	0.0
16	2.024939150578525E-4	0.0	0.0	27.601135585875646	0.0
17	2.024939150578525E-4	0.0	0.0	29.729954114878847	0.0
18	2.024939150578525E-4	0.0	0.0	31.814426476484382	0.0
19	2.024939150578525E-4	0.0	0.0	33.47710401302441	0.0
20	2.024939150578525E-4	0.0	0.0	34.70826701657615	0.0
21	2.024939150578525E-4	0.0	0.0	36.19335738961044	0.0
22	2.024939150578525E-4	0.0	0.0	37.78657951328562	0.0
23	2.024939150578525E-4	0.0	0.0	39.27288484981026	0.0
24	2.024939150578525E-4	0.0	0.0	40.27543222326169	0.0
25	2.024939150578525E-4	0.0	0.0	41.121046812543284	0.0
26	2.024939150578525E-4	0.0	0.0	41.80304631845813	0.0
27	2.024939150578525E-4	0.0	0.0	42.445357017021635	0.0
28	2.024939150578525E-4	0.0	0.0	43.044334017762765	0.0
29	2.024939150578525E-4	0.0	0.0	43.64351351241895	0.0
30	2.024939150578525E-4	0.0	0.0	44.23216332349213	0.0
31	2.024939150578525E-4	0.0	0.0	44.73029835453445	0.0
32	2.024939150578525E-4	0.0	0.0	45.186719639074845	0.0
33	2.024939150578525E-4	0.0	0.0	45.61175436678128	0.0
34	2.024939150578525E-4	0.0	0.0	45.98859554270394	0.0
35	2.024939150578525E-4	0.0	0.0	46.40472053814783	0.0
36	2.024939150578525E-4	0.0	0.0	46.803633550811796	0.0
37	2.024939150578525E-4	0.0	0.0	47.22036602800086	0.0
38	2.024939150578525E-4	0.0	0.0	47.617659089344365	0.0
39	2.024939150578525E-4	0.0	0.0	47.97809825814734	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAA	35	1.2100463E-7	45.000004	33
TTAGCGG	35	1.2100463E-7	45.000004	2
TACGGGT	70	0.0	45.000004	4
CCATCGA	60	0.0	45.000004	41
TCGTAAC	35	1.2100463E-7	45.000004	34
CAGCGTG	20	7.029374E-4	45.0	1
ACCGGGT	20	7.029374E-4	45.0	4
GCGACCG	40	6.8012014E-9	45.0	33
TTAGACG	20	7.029374E-4	45.0	1
GTTAGCG	20	7.029374E-4	45.0	1
CGTTATT	465	0.0	45.0	1
ACGATAG	20	7.029374E-4	45.0	1
TGACCGG	20	7.029374E-4	45.0	2
CTACGAA	20	7.029374E-4	45.0	11
CGAATAT	20	7.029374E-4	45.0	14
TCCGTAG	20	7.029374E-4	45.0	1
GGGCCGA	45	3.8380676E-10	45.0	7
TACACGC	20	7.029374E-4	45.0	35
TGCACGG	50	2.1827873E-11	45.0	2
AGTAACG	20	7.029374E-4	45.0	1
>>END_MODULE