SRR2935243.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935243.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	385859
Sequences flagged as poor quality	0
Sequence length	51
%GC	41

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8356	2.165557885134207	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5021	1.301252530069274	No Hit
GCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	3662	0.9490513374056326	TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCG	3207	0.8311326158000721	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTC	2917	0.7559756284031214	No Hit
CCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	2666	0.690925960000933	TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCC	2516	0.652051656174924	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGC	2359	0.6113632181703679	No Hit
CTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGCT	1896	0.4913712003607535	TruSeq Adapter, Index 13 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1758	0.45560684084082526	No Hit
TCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	1376	0.3566069470972557	TruSeq Adapter, Index 19 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	946	0.2451672761293633	TruSeq Adapter, Index 13 (95% over 21bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	826	0.21406783306855615	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	824	0.2135495090175427	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCT	767	0.19877727356365926	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCG	757	0.196185653308592	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGTTCACT	682	0.1767485013955875	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	584	0.15135062289592832	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	544	0.14098414187565925	No Hit
ACCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG	538	0.13942916972261887	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	530	0.13735587351856507	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGC	522	0.13528257731451127	No Hit
GAACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCT	509	0.1319134709829238	No Hit
GCCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG	502	0.13009933680437671	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTC	480	0.12439777224322875	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	464	0.12025117983512111	No Hit
AGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG	461	0.11947369375860094	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTC	443	0.11480877729947987	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTA	433	0.1122171570444126	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGT	417	0.10807056463630496	No Hit
CGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG	404	0.10470145830471751	No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	402	0.10418313425370408	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTA	60	0.0	45.000004	5
CGTTAGG	30	2.1617398E-6	45.000004	2
TAACATG	30	2.1617398E-6	45.000004	36
TCGAAGG	60	0.0	45.000004	2
CTATGGT	20	7.0274563E-4	45.0	11
CGGGTAC	25	3.8859725E-5	45.0	6
GGAGCGA	20	7.0274563E-4	45.0	9
GCGAAGG	20	7.0274563E-4	45.0	2
CATGACG	20	7.0274563E-4	45.0	1
CGTGAGG	20	7.0274563E-4	45.0	2
GCTATTG	20	7.0274563E-4	45.0	1
TTAGACG	20	7.0274563E-4	45.0	1
ATATGCA	20	7.0274563E-4	45.0	13
TCGATGG	20	7.0274563E-4	45.0	2
ATGTCCG	20	7.0274563E-4	45.0	11
GTAGCAC	20	7.0274563E-4	45.0	9
TAACCGC	20	7.0274563E-4	45.0	24
CGGGCTA	25	3.8859725E-5	45.0	6
CGGGCAT	35	1.2091186E-7	45.0	6
GCGATGT	20	7.0274563E-4	45.0	9

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content