##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2935243.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 385859 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.55973814268943 31.0 31.0 34.0 30.0 34.0 2 31.66429447025986 31.0 31.0 34.0 30.0 34.0 3 31.687541303947814 31.0 31.0 34.0 30.0 34.0 4 35.50761288449926 37.0 35.0 37.0 33.0 37.0 5 32.93277596220381 37.0 35.0 37.0 28.0 37.0 6 33.95649965401869 36.0 35.0 37.0 27.0 37.0 7 35.26420791014334 37.0 35.0 37.0 32.0 37.0 8 35.15220067433959 37.0 35.0 37.0 32.0 37.0 9 37.219847146237356 39.0 37.0 39.0 34.0 39.0 10 36.7434088617863 39.0 37.0 39.0 32.0 39.0 11 36.58990460245841 39.0 35.0 39.0 32.0 39.0 12 36.960560722958384 39.0 37.0 39.0 33.0 39.0 13 36.91814885748421 39.0 37.0 39.0 33.0 39.0 14 37.81826781285392 39.0 37.0 41.0 33.0 41.0 15 37.92733096804791 40.0 37.0 41.0 33.0 41.0 16 37.9347663265597 40.0 37.0 41.0 33.0 41.0 17 37.879665888316715 39.0 37.0 41.0 33.0 41.0 18 37.77991442469918 39.0 37.0 40.0 33.0 41.0 19 37.57190061654646 39.0 36.0 40.0 33.0 41.0 20 37.327780355000144 39.0 35.0 40.0 33.0 41.0 21 37.256687028163135 39.0 35.0 40.0 33.0 41.0 22 37.23118030161277 39.0 35.0 40.0 33.0 41.0 23 36.930803739189706 39.0 35.0 40.0 32.0 41.0 24 36.63537717145382 38.0 35.0 40.0 31.0 41.0 25 36.43336814743209 38.0 35.0 40.0 31.0 41.0 26 36.756960444100045 38.0 35.0 40.0 32.0 41.0 27 36.52087161372418 38.0 35.0 40.0 31.0 41.0 28 36.64803205315931 38.0 35.0 40.0 31.0 41.0 29 36.56943080244338 38.0 35.0 40.0 31.0 41.0 30 36.23601367338846 38.0 35.0 40.0 30.0 41.0 31 36.154717137607264 38.0 35.0 40.0 30.0 41.0 32 35.73662659157879 38.0 35.0 40.0 30.0 41.0 33 35.707292041911685 38.0 35.0 40.0 29.0 41.0 34 35.63950043927964 38.0 35.0 40.0 29.0 41.0 35 35.316260602966366 38.0 35.0 40.0 25.0 41.0 36 35.22324476039175 38.0 35.0 40.0 25.0 41.0 37 35.053413293456934 38.0 35.0 40.0 24.0 41.0 38 34.95094062857158 38.0 35.0 40.0 24.0 41.0 39 34.94543343552956 38.0 35.0 40.0 23.0 41.0 40 34.88277324100254 38.0 35.0 40.0 23.0 41.0 41 34.77656864294988 38.0 34.0 40.0 23.0 41.0 42 34.86746713177612 38.0 35.0 40.0 23.0 41.0 43 34.7554287965293 38.0 34.0 40.0 23.0 41.0 44 34.6541871512651 38.0 34.0 40.0 22.0 41.0 45 34.629670423652165 38.0 34.0 40.0 22.0 41.0 46 34.4615261015034 38.0 34.0 40.0 22.0 41.0 47 34.36859837401745 38.0 34.0 40.0 22.0 41.0 48 34.35185132392921 38.0 34.0 40.0 22.0 41.0 49 34.41241748929013 38.0 34.0 40.0 22.0 41.0 50 34.3203942372732 38.0 34.0 40.0 20.0 41.0 51 33.33633529346212 36.0 32.0 40.0 19.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 4.0 9 3.0 10 8.0 11 12.0 12 21.0 13 25.0 14 24.0 15 40.0 16 52.0 17 117.0 18 179.0 19 404.0 20 646.0 21 1145.0 22 1654.0 23 2387.0 24 3415.0 25 4758.0 26 5973.0 27 6853.0 28 7135.0 29 7442.0 30 8266.0 31 9984.0 32 12768.0 33 16986.0 34 24748.0 35 36747.0 36 45136.0 37 57911.0 38 80198.0 39 50809.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.229920774168804 22.629509743196348 22.525844932993657 17.61472454964119 2 34.2283061947499 26.995094062857156 22.831137799040583 15.945461943352365 3 24.977776856312797 26.941447523577267 34.431748384772675 13.649027235337261 4 23.43913191087936 23.799107964308206 36.95417237902964 15.807587745782786 5 19.547554935870355 32.868483046916104 30.724176447873447 16.859785569340097 6 19.609494659966465 34.09120948325683 33.258262733278215 13.04103312349848 7 60.434770213990085 9.302880067589456 24.14716256456374 6.115187153856719 8 57.21882863947712 10.997022228326928 22.858868135769804 8.925280996426157 9 52.043622152133295 10.420905045625474 25.313650841369515 12.221821960871718 10 28.573909122244135 24.581258957287506 31.87952075758243 14.965311162885925 11 18.809720649252707 24.325984362163382 35.9854247276855 20.87887026089841 12 17.875700709326463 21.059765354702105 41.40009692659754 19.66443700937389 13 18.93074931516435 22.670975667277425 43.30234619381691 15.095928823741314 14 14.415628506786158 27.2317089921448 38.802256782918114 19.55040571815093 15 11.503424826167072 25.75733622903703 44.05002863740382 18.68921030739208 16 11.736152325072112 27.368287379586842 37.327365695759326 23.568194599581712 17 11.932337978380705 27.524562080967403 36.95805980941225 23.585040131239648 18 11.908754234059591 25.911278472188027 42.20583166389795 19.974135629854427 19 12.993088148779735 27.401460118851706 38.228212896420715 21.377238835947846 20 13.69023399739283 29.113225297323638 40.31628133592841 16.880259369355127 21 14.092194298953764 29.725366001570524 38.99973824635424 17.182701453121478 22 11.168328327186874 27.24492625544564 36.47964671032683 25.107098707040652 23 11.802238641576327 27.549182473390537 37.61814548837788 23.030433396655255 24 15.871859928108453 25.37325810723606 35.08405920297311 23.670822761682373 25 12.181651846918175 30.49429973124898 33.23545647503362 24.08859194679922 26 14.231623468676382 28.226372846039617 35.86284108962082 21.679162595663183 27 16.262676262572597 30.509331128728366 33.9165342780653 19.31145833063373 28 13.932809653267125 26.863698915925248 35.86439606177386 23.339095369033767 29 18.437823142650554 24.713690752321444 35.75399303890799 21.094493066120005 30 18.0659256360484 30.126548817054932 32.89776835579836 18.909757191098304 31 18.230493522245172 31.073786020282018 28.076836357322232 22.618884100150574 32 17.478923648275664 31.087780769659386 30.440134867918072 20.993160714146878 33 16.44227554624876 26.956478921056654 31.03750333671108 25.563742195983508 34 18.632972147857117 24.055160045508853 31.164492729209375 26.147375077424655 35 17.35659917223649 29.142769768231403 29.260688489836962 24.239942569695145 36 21.12740664335936 28.569244205785015 28.095755185184224 22.2075939656714 37 18.432380740114912 28.47542755255158 32.909171484920655 20.18302022241285 38 17.455339903954552 28.28442513975312 32.04149702352414 22.21873793276819 39 18.474105826221496 22.031882112377836 35.20120043850214 24.29281162289852 40 18.7516683555392 24.729240473851846 33.00817137866422 23.510919791944726 41 17.316169896257442 29.663685439499922 25.23875301599807 27.781391648244565 42 19.781059920851916 26.541560518220386 29.405041738044208 24.27233782288349 43 21.82818076032955 24.00384596445852 31.011068810109393 23.15690446510254 44 20.30093894401841 22.222884525176294 32.10473255774778 25.371443973057517 45 21.214225921904113 20.294200731355236 31.413029111670326 27.078544235070325 46 22.434101575964277 24.172042119012385 32.46237615294706 20.931480152076276 47 15.032693289517674 25.12264842857106 37.05394975884973 22.790708523061536 48 16.709212432520687 23.511956440046752 33.480882913188495 26.297948214244066 49 19.038042393724133 19.20209195586989 37.818736896120086 23.94112875428589 50 18.865958808787667 18.227124415913583 34.85729243065472 28.04962434464403 51 16.983665017532314 18.620014046581783 30.181232004436854 34.21508893144905 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6154.0 1 6407.0 2 6660.0 3 10725.0 4 14790.0 5 9670.0 6 4550.0 7 4379.5 8 4209.0 9 4549.5 10 4890.0 11 4803.0 12 4716.0 13 4701.5 14 4687.0 15 4116.5 16 3546.0 17 3244.0 18 2942.0 19 2977.0 20 3012.0 21 3222.0 22 3432.0 23 3308.5 24 3185.0 25 3328.0 26 3623.0 27 3775.0 28 3721.5 29 3668.0 30 3974.5 31 4281.0 32 4642.5 33 5004.0 34 5336.5 35 5669.0 36 5993.0 37 6317.0 38 6954.0 39 7591.0 40 8917.5 41 10244.0 42 13985.0 43 17726.0 44 21687.0 45 25648.0 46 36649.5 47 47651.0 48 51004.0 49 54357.0 50 52327.5 51 50298.0 52 39085.0 53 27872.0 54 21308.0 55 14744.0 56 11565.0 57 8386.0 58 6996.5 59 5607.0 60 5197.5 61 4788.0 62 4327.5 63 3867.0 64 3374.5 65 2882.0 66 2371.5 67 1861.0 68 1540.5 69 1220.0 70 1080.5 71 941.0 72 798.5 73 656.0 74 459.5 75 234.5 76 206.0 77 133.0 78 60.0 79 38.0 80 16.0 81 11.0 82 6.0 83 5.0 84 4.0 85 3.5 86 3.0 87 2.5 88 2.0 89 1.5 90 1.0 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 385859.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.712695776244345 #Duplication Level Percentage of deduplicated Percentage of total 1 71.40690892527007 26.92947032620697 2 11.10730582125755 8.377728906615875 3 4.901259816799706 5.545191611738951 4 2.7958019749995007 4.21748917335117 5 1.9388986146077862 3.656054679684253 6 1.4128984087207432 3.197052471049507 7 1.097297111351072 2.8967422495574287 8 0.8512224876965768 2.5681515773119115 9 0.7434255947865722 2.5232924959613547 >10 3.551664523940888 20.854817119212264 >50 0.10956940479932993 2.780389375725912 >100 0.0669978526161508 4.803304250946412 >500 0.009072625875103754 2.2453144419081554 >1k 0.006281048682764137 5.884245103474462 >5k 0.0013957885961698082 3.520756217255351 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8356 2.165557885134207 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5021 1.301252530069274 No Hit GCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC 3662 0.9490513374056326 TruSeq Adapter, Index 19 (95% over 21bp) GAATGATACCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCG 3207 0.8311326158000721 No Hit GAATCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTC 2917 0.7559756284031214 No Hit CCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC 2666 0.690925960000933 TruSeq Adapter, Index 19 (95% over 21bp) CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCC 2516 0.652051656174924 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGC 2359 0.6113632181703679 No Hit CTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGCT 1896 0.4913712003607535 TruSeq Adapter, Index 13 (95% over 22bp) GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1758 0.45560684084082526 No Hit TCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC 1376 0.3566069470972557 TruSeq Adapter, Index 19 (95% over 21bp) ACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC 946 0.2451672761293633 TruSeq Adapter, Index 13 (95% over 21bp) TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 826 0.21406783306855615 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 824 0.2135495090175427 No Hit GAATGACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCT 767 0.19877727356365926 No Hit CGTTTTTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCG 757 0.196185653308592 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGTTCACT 682 0.1767485013955875 No Hit CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 584 0.15135062289592832 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 544 0.14098414187565925 No Hit ACCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG 538 0.13942916972261887 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 530 0.13735587351856507 No Hit CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGC 522 0.13528257731451127 No Hit GAACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCT 509 0.1319134709829238 No Hit GCCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG 502 0.13009933680437671 No Hit GAATGATCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTC 480 0.12439777224322875 No Hit TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC 464 0.12025117983512111 No Hit AGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG 461 0.11947369375860094 No Hit CGTTTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTC 443 0.11480877729947987 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTA 433 0.1122171570444126 No Hit CGTTTTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGT 417 0.10807056463630496 No Hit CGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG 404 0.10470145830471751 No Hit GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 402 0.10418313425370408 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.5299863421612558 0.0 2 0.0 0.0 0.0 2.953151280649149 0.0 3 0.0 0.0 0.0 3.9268230104779205 0.0 4 0.0 0.0 0.0 5.259952469684522 0.0 5 0.0 0.0 0.0 9.102806983898263 0.0 6 0.0 0.0 0.0 10.84644909150752 0.0 7 0.0 0.0 0.0 12.959915409514874 0.0 8 0.0 0.0 0.0 15.787113945767755 0.0 9 0.0 0.0 0.0 16.80795316423875 0.0 10 0.0 0.0 0.0 20.452807890965353 0.0 11 0.0 0.0 0.0 25.257412681834555 0.0 12 0.0 0.0 0.0 29.229329884750648 0.0 13 0.0 0.0 0.0 30.524880850258775 0.0 14 0.0 0.0 0.0 31.067825293695364 0.0 15 0.0 0.0 0.0 32.17341049450706 0.0 16 0.0 0.0 0.0 34.35918301763079 0.0 17 0.0 0.0 0.0 36.92644204230042 0.0 18 0.0 0.0 0.0 39.67796526710534 0.0 19 0.0 0.0 0.0 41.55196587354448 0.0 20 0.0 0.0 0.0 43.148403950665916 0.0 21 0.0 0.0 0.0 44.88867695194359 0.0 22 0.0 0.0 0.0 46.61599185194592 0.0 23 0.0 0.0 0.0 48.05926517199288 0.0 24 0.0 0.0 0.0 49.08969338540762 0.0 25 0.0 0.0 0.0 49.90916371005989 0.0 26 0.0 0.0 0.0 50.633521571351196 0.0 27 0.0 0.0 0.0 51.21534031861379 0.0 28 0.0 0.0 0.0 51.81815118994244 0.0 29 0.0 0.0 0.0 52.36705635996569 0.0 30 0.0 0.0 0.0 52.93488035785092 0.0 31 0.0 0.0 0.0 53.42469658605864 0.0 32 0.0 0.0 0.0 53.82458359141552 0.0 33 0.0 0.0 0.0 54.22732137905297 0.0 34 0.0 0.0 0.0 54.59584977932354 0.0 35 0.0 0.0 0.0 54.939498625145454 0.0 36 0.0 0.0 0.0 55.28314747096737 0.0 37 0.0 0.0 0.0 55.586367040810245 0.0 38 2.5916202550672657E-4 0.0 0.0 55.90176722585193 0.0 39 2.5916202550672657E-4 0.0 0.0 56.213798304562026 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGGGTA 60 0.0 45.000004 5 CGTTAGG 30 2.1617398E-6 45.000004 2 TAACATG 30 2.1617398E-6 45.000004 36 TCGAAGG 60 0.0 45.000004 2 CTATGGT 20 7.0274563E-4 45.0 11 CGGGTAC 25 3.8859725E-5 45.0 6 GGAGCGA 20 7.0274563E-4 45.0 9 GCGAAGG 20 7.0274563E-4 45.0 2 CATGACG 20 7.0274563E-4 45.0 1 CGTGAGG 20 7.0274563E-4 45.0 2 GCTATTG 20 7.0274563E-4 45.0 1 TTAGACG 20 7.0274563E-4 45.0 1 ATATGCA 20 7.0274563E-4 45.0 13 TCGATGG 20 7.0274563E-4 45.0 2 ATGTCCG 20 7.0274563E-4 45.0 11 GTAGCAC 20 7.0274563E-4 45.0 9 TAACCGC 20 7.0274563E-4 45.0 24 CGGGCTA 25 3.8859725E-5 45.0 6 CGGGCAT 35 1.2091186E-7 45.0 6 GCGATGT 20 7.0274563E-4 45.0 9 >>END_MODULE