FastQCFastQC Report
Sat 14 Jan 2017
SRR2935241.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935241.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6402709
Sequences flagged as poor quality0
Sequence length51
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5008277.82211092211125No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTT115080.17973642094307268No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCC113740.17764355681321764No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGT110100.17195846320674577No Hit
GAATCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTC108890.17006863813426473No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCT97530.15232614819758325No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCG94540.14765624987798132No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA91430.1427989308900342No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTC79460.12410371922259782No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA78970.12333841816018812No Hit
CGTTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTC76770.11990237257385897No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA70990.11087494371523055No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC67350.10518985010875864No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA64510.10075422762458829No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT2218200.044.588181
CGTTATT170100.044.5370371
GTTATTT191800.039.7797162
GTTTTTT2514350.039.3543052
GCGTAAG4100.038.4146351
CGTAAGG8000.037.68752
CGTTGTT7400.036.7905431
ATCGACG900.035.01
TAAGGGA59500.034.7899174
CGGTCTA2600.034.61538331
TATCGCG1300.034.6153831
AGGGATC49650.034.169186
AAGGGAT89050.033.882655
GGTACGA1600.033.758
TAGGGAT63950.033.7412035
TATGGGC12250.033.6122444
ACGGGAC20850.033.561155
CGTTTCT19550.033.260871
GGGCGAT64100.033.065527
CTACGAA6750.032.66666811
Produced by FastQC (version 0.11.5)