FastQCFastQC Report
Sat 14 Jan 2017
SRR2935240.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935240.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331550
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88352.664756446991404No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC20290.6119740612275675TruSeq Adapter, Index 15 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCC19770.5962901523148846No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19360.5839239933645001No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGC17070.5148544714221083No Hit
CCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC17040.5139496305232997TruSeq Adapter, Index 15 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCG16330.4925350625848288No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTC15740.47473985824159254No Hit
CTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGCT11040.3329814507615744TruSeq Adapter, Index 14 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8220.24792640627356358No Hit
TCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC7630.23013120193032724TruSeq Adapter, Index 15 (95% over 23bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7390.22289247473985824No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7070.21324083848589956No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5970.1800633388629166No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCG5600.1689036344442769No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5450.16437942995023375No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGC4630.13964711204946464No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCAGTAT4400.13270999849193185No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4050.12215352133916452No Hit
ACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC4010.12094706680741969TruSeq Adapter, Index 14 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCT3500.10556477152767305No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTATG253.8847807E-545.0000049
GCTACGA502.1827873E-1145.00000410
CCTATGT253.8847807E-545.00000410
TAGCTAG253.8847807E-545.0000041
CGGGTAT207.0260186E-445.06
TAATACG406.7902874E-945.04
ACACGAC207.0260186E-445.026
GATCGTT351.2084274E-745.09
ATTACGG207.0260186E-445.02
CGCTGTT207.0260186E-445.044
TAGTTCG207.0260186E-445.01
GTTCGGG453.8380676E-1045.03
CATACGG207.0260186E-445.02
CTCGCGG207.0260186E-445.02
ATGGTCA207.0260186E-445.013
ACGTATG207.0260186E-445.01
TGGGCGA406.7902874E-945.06
CACGTGA207.0260186E-445.043
TCAAGCG207.0260186E-445.017
TTCGAAT207.0260186E-445.031
Produced by FastQC (version 0.11.5)