SRR2935238.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935238.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	619428
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10119	1.6336039055386582	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCG	8271	1.3352641469226447	No Hit
GCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	7962	1.2853794145566555	TruSeq Adapter, Index 16 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTC	7387	1.1925518381474522	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGC	6485	1.0469336226324932	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5190	0.8378697766326352	No Hit
CCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	4612	0.7445578824334709	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGCT	3632	0.5863474043795243	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCC	3209	0.5180585959950148	No Hit
TCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	2564	0.41393027115338665	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCT	2081	0.3359551069696559	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1806	0.2915593095565586	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTGTCTTT	1736	0.28025856112413383	No Hit
ACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	1627	0.26266168142221535	TruSeq Adapter, Index 13 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCT	1482	0.23925298824076405	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTA	1273	0.20551218220681017	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTC	1192	0.19243560187786152	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCG	1028	0.16595956269332351	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	982	0.15853335658058726	No Hit
ACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTG	925	0.1493313185713271	No Hit
GCCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTG	872	0.14077503761534835	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGC	708	0.11429899843081036	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	682	0.11010157758448116	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	669	0.10800286716131657	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTG	661	0.10671135305475375	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	659	0.10638847452811304	No Hit
GGCCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCT	652	0.10525839968487055	No Hit
AGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTG	628	0.10138385736518207	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGG	40	6.8066583E-9	45.000004	3
CCTTCGA	25	3.8887145E-5	45.000004	41
CTTCGAA	20	7.0307637E-4	45.000004	42
AACGTAG	30	2.1638753E-6	45.000004	1
CTCACGG	20	7.0307637E-4	45.000004	2
TGCCCGT	20	7.0307637E-4	45.000004	31
ACCGGAC	20	7.0307637E-4	45.000004	4
GCGACCG	20	7.0307637E-4	45.000004	33
GACGTTG	25	3.8887145E-5	45.000004	1
GTCTACG	20	7.0307637E-4	45.000004	1
CGATTGA	20	7.0307637E-4	45.000004	10
CTAAGCG	20	7.0307637E-4	45.000004	1
ACGGGTA	30	2.1638753E-6	45.000004	5
TCGATAG	25	3.8887145E-5	45.000004	1
ACGGGGT	160	0.0	45.000004	5
TGTTGCG	40	6.8066583E-9	45.000004	1
TACGCAG	60	0.0	45.000004	1
GCTTACG	30	2.1638753E-6	45.000004	1
CGGGCTC	20	7.0307637E-4	45.000004	6
TCGCTAA	25	3.8887145E-5	45.000004	20

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content