##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935238.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	619428
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.44790839290442	31.0	31.0	34.0	30.0	34.0
2	31.53368914546969	31.0	31.0	34.0	30.0	34.0
3	31.586069728846613	31.0	31.0	34.0	30.0	34.0
4	35.43956359738339	37.0	35.0	37.0	33.0	37.0
5	32.8876802469375	35.0	35.0	37.0	28.0	37.0
6	33.865569525433145	35.0	35.0	37.0	26.0	37.0
7	35.23785008104251	37.0	35.0	37.0	32.0	37.0
8	35.069807951852354	37.0	35.0	37.0	32.0	37.0
9	37.122006108861726	39.0	37.0	39.0	34.0	39.0
10	36.64196645937865	39.0	35.0	39.0	32.0	39.0
11	36.4590121854356	39.0	35.0	39.0	32.0	39.0
12	36.892526653622376	39.0	37.0	39.0	33.0	39.0
13	36.87364310299179	39.0	37.0	39.0	33.0	39.0
14	37.74372969901264	39.0	37.0	41.0	33.0	41.0
15	37.85409442259633	39.0	37.0	41.0	33.0	41.0
16	37.85428007774915	39.0	37.0	41.0	33.0	41.0
17	37.81783354966195	39.0	37.0	41.0	33.0	41.0
18	37.77631944309912	39.0	37.0	40.0	33.0	41.0
19	37.633825077329405	39.0	37.0	40.0	33.0	41.0
20	37.42204905170577	39.0	35.0	40.0	32.0	41.0
21	37.39035529553072	39.0	35.0	40.0	33.0	41.0
22	37.37722866903014	39.0	35.0	40.0	33.0	41.0
23	37.06280794539478	39.0	35.0	40.0	32.0	41.0
24	36.80378187618254	39.0	35.0	40.0	31.0	41.0
25	36.57586676740477	38.0	35.0	40.0	31.0	41.0
26	36.98174606249637	39.0	35.0	40.0	32.0	41.0
27	36.87331053810935	39.0	35.0	40.0	32.0	41.0
28	36.99613998721401	39.0	35.0	40.0	32.0	41.0
29	37.12503793822688	39.0	35.0	40.0	32.0	41.0
30	36.81663728472074	39.0	35.0	40.0	31.0	41.0
31	36.775602652769976	39.0	35.0	40.0	31.0	41.0
32	36.5936444590816	39.0	35.0	40.0	31.0	41.0
33	36.524982725998825	39.0	35.0	40.0	31.0	41.0
34	36.47009014768464	39.0	35.0	40.0	30.0	41.0
35	36.31409784510871	39.0	35.0	40.0	30.0	41.0
36	36.170949650322555	39.0	35.0	40.0	30.0	41.0
37	36.14618002415131	39.0	35.0	40.0	30.0	41.0
38	36.007248622923086	39.0	35.0	40.0	29.0	41.0
39	35.93316737377064	39.0	35.0	40.0	29.0	41.0
40	35.802598526382404	39.0	35.0	40.0	28.0	41.0
41	35.70544276332358	39.0	35.0	40.0	27.0	41.0
42	35.88231723460999	39.0	35.0	40.0	28.0	41.0
43	35.76102307289951	39.0	35.0	40.0	27.0	41.0
44	35.62847498014297	39.0	35.0	40.0	26.0	41.0
45	35.550714530179455	39.0	35.0	40.0	26.0	41.0
46	35.405152818406656	39.0	35.0	40.0	26.0	41.0
47	35.316629212757576	39.0	35.0	40.0	25.0	41.0
48	35.304782799615126	39.0	35.0	40.0	25.0	41.0
49	35.352867484195095	39.0	35.0	40.0	26.0	41.0
50	35.220529262480866	38.0	35.0	40.0	25.0	41.0
51	34.1915961177086	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	9.0
10	9.0
11	13.0
12	10.0
13	14.0
14	23.0
15	34.0
16	76.0
17	121.0
18	250.0
19	491.0
20	863.0
21	1590.0
22	2640.0
23	3636.0
24	4752.0
25	6136.0
26	7912.0
27	8833.0
28	9267.0
29	9858.0
30	11296.0
31	13992.0
32	18584.0
33	24432.0
34	35687.0
35	52511.0
36	68058.0
37	92577.0
38	140282.0
39	105459.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.32054088610783	21.796560697934222	22.211782483194174	14.671115932763776
2	32.19696235882136	29.632015343187586	23.308600838192657	14.862421459798394
3	25.316420956107894	29.57841750776523	32.07087829416817	13.034283241958711
4	23.222069393052948	24.276429221798175	37.16719295866509	15.334308426483789
5	21.76281989190027	32.536630568847386	29.370645175871935	16.32990436338041
6	19.81376366583364	36.559051253737316	30.741425960725056	12.885759119703986
7	63.71329678348412	7.4442873102281455	23.027373641488598	5.815042264799137
8	59.36589886153032	11.476071472390657	19.729976688170375	9.428052977908651
9	54.45717662101164	9.388661797658484	22.294762264540836	13.859399316789037
10	28.576041121809155	29.03178416216251	28.886004507384232	13.506170208644102
11	17.128382959762877	23.4295188464196	38.46306592533757	20.97903226847995
12	16.365259562047566	20.706199913468552	42.69697204517716	20.231568479306716
13	18.407466243050038	23.16072247299121	44.582259762232255	13.849551521726497
14	14.4635050401338	28.527286464286405	37.03206829526596	19.97714020031384
15	11.229876595827118	26.449724584616774	43.81041864429764	18.509980175258463
16	11.175310124824838	28.284481812252594	35.83225169026908	24.70795637265348
17	11.621689687905617	28.589602019928062	36.43894044182698	23.349767850339344
18	11.446366647939712	25.970088533292007	42.02409319565793	20.559451623110352
19	11.584397218078614	27.643567936870788	38.173928204730814	22.598106640319777
20	12.988111612649089	29.732914882762806	40.861569060488065	16.41740444410004
21	13.724597531916544	30.76564185022311	38.3657180495554	17.14404256830495
22	10.567975616213667	27.185241868304306	35.95123242733619	26.295550088145838
23	11.658820718469297	28.160980775812522	36.32706303234597	23.85313547337221
24	15.986200171771376	25.09024454819608	33.829759068043415	25.09379621198913
25	11.12994569183182	32.08040321070407	31.62821183414376	25.16143926332035
26	14.192448517018926	27.133581304041794	35.82111238109999	22.852857797839295
27	15.91000083948417	27.006851482335314	33.79860129022259	23.28454638795792
28	11.545006037828449	25.08701576292967	38.561705315226305	24.806272884015577
29	20.69877370735582	22.42165352551063	32.91617427691354	23.96339849022001
30	16.846187127478903	27.161994614386174	32.97881271108184	23.013005547053087
31	16.323769671374237	26.984088546207147	30.686052293406174	26.006089489012446
32	20.400756827266445	27.158281511329808	30.23499099168911	22.205970669714638
33	15.097154148666188	22.287013179901457	35.32161930038681	27.294213371045544
34	18.629768108642168	20.76851546911021	33.760985941868945	26.84073048037867
35	18.673841027528624	20.27531851966653	33.08261815739682	27.968222295408022
36	18.480113911544198	21.04506092717798	37.24129358052913	23.23353158074869
37	17.19747896446399	21.57280587897221	37.975519350110105	23.254195806453694
38	17.79254408906281	22.983946479655422	36.889840304280725	22.33366912700104
39	22.13251580490388	19.82312714310622	36.0932989790581	21.9510580729318
40	19.428408144287957	18.483342696810606	37.593069735304184	24.495179423597254
41	16.64503380538174	21.636412948720434	33.83072770362334	27.887825542274484
42	18.707097515772617	18.199371032630104	40.19030460360204	22.90322684799525
43	22.649605765318974	18.287516870403017	36.46557792027483	22.597299444003177
44	20.37686381629503	18.41795979516586	33.90902574633372	27.29615064220539
45	21.614618648172186	16.201398709777408	33.01465222753895	29.169330414511453
46	24.10917814499829	18.81929780378026	35.05798898338467	22.013535067836777
47	14.705825374377651	18.934888316317636	43.77054314625751	22.588743163047198
48	17.15695770937058	18.254098942895705	38.25190336891455	26.33703997881917
49	19.530922076496378	14.542933157687415	43.61992031357963	22.306224452236577
50	19.940170609013478	14.291087906907663	37.75047301704153	28.018268467037334
51	18.17547802165869	14.35727800486901	32.88065763898306	34.58658633448924
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6111.0
1	6334.5
2	6558.0
3	11574.0
4	16590.0
5	10795.0
6	5000.0
7	5052.0
8	5104.0
9	5458.0
10	5812.0
11	6294.0
12	6776.0
13	6759.0
14	6742.0
15	6336.5
16	5931.0
17	5503.5
18	5076.0
19	5263.5
20	5451.0
21	5595.5
22	5740.0
23	5579.5
24	5419.0
25	5749.0
26	6446.0
27	6813.0
28	7166.5
29	7520.0
30	8065.5
31	8611.0
32	8942.5
33	9274.0
34	10015.5
35	10757.0
36	11212.0
37	11667.0
38	12502.0
39	13337.0
40	15838.0
41	18339.0
42	24038.5
43	29738.0
44	36313.5
45	42889.0
46	68046.5
47	93204.0
48	94062.5
49	94921.0
50	90449.5
51	85978.0
52	64517.0
53	43056.0
54	31898.0
55	20740.0
56	15659.5
57	10579.0
58	8342.0
59	6105.0
60	5299.0
61	4493.0
62	3824.5
63	3156.0
64	2701.0
65	2246.0
66	1835.0
67	1424.0
68	1140.5
69	857.0
70	658.5
71	460.0
72	417.5
73	375.0
74	319.0
75	187.0
76	111.0
77	95.0
78	79.0
79	51.5
80	24.0
81	18.0
82	12.0
83	9.0
84	6.0
85	4.5
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	619428.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.84347540502487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.06576891379436	23.374256515631302
2	11.677606634482288	7.904215858473131
3	5.447005449265405	5.530367848597505
4	3.1498661429256813	4.2640966934890345
5	2.099301982655104	3.552383750885398
6	1.4431857720173233	2.930545330809007
7	1.1731150818144636	2.7791603993056073
8	0.9254302061035207	2.505581953546528
9	0.7416580912899476	2.259025863435813
>10	4.028507516008082	22.34737009195189
>50	0.14458698187568433	3.35610152387084
>100	0.0865587617232534	5.518166049652108
>500	0.008704233022450063	1.9517660688104586
>1k	0.0058028220149667084	4.294670902039582
>5k	0.0024178425062361284	5.776252171613713
>10k+	4.8356850124722567E-4	1.6560389778880622
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10119	1.6336039055386582	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCG	8271	1.3352641469226447	No Hit
GCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	7962	1.2853794145566555	TruSeq Adapter, Index 16 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTC	7387	1.1925518381474522	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGC	6485	1.0469336226324932	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5190	0.8378697766326352	No Hit
CCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	4612	0.7445578824334709	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGCT	3632	0.5863474043795243	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCC	3209	0.5180585959950148	No Hit
TCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	2564	0.41393027115338665	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCT	2081	0.3359551069696559	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1806	0.2915593095565586	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTGTCTTT	1736	0.28025856112413383	No Hit
ACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	1627	0.26266168142221535	TruSeq Adapter, Index 13 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCT	1482	0.23925298824076405	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTA	1273	0.20551218220681017	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTC	1192	0.19243560187786152	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCG	1028	0.16595956269332351	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	982	0.15853335658058726	No Hit
ACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTG	925	0.1493313185713271	No Hit
GCCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTG	872	0.14077503761534835	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGC	708	0.11429899843081036	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	682	0.11010157758448116	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	669	0.10800286716131657	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTG	661	0.10671135305475375	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	659	0.10638847452811304	No Hit
GGCCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCT	652	0.10525839968487055	No Hit
AGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTG	628	0.10138385736518207	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.2287852664070725E-4	0.0	0.0	0.6200882104134783	0.0
2	3.2287852664070725E-4	0.0	0.0	3.5024248177350716	0.0
3	3.2287852664070725E-4	0.0	0.0	4.507061353377632	0.0
4	3.2287852664070725E-4	0.0	0.0	6.048322000297048	0.0
5	3.2287852664070725E-4	0.0	0.0	10.46885190853497	0.0
6	3.2287852664070725E-4	0.0	0.0	12.218853522927605	0.0
7	3.2287852664070725E-4	0.0	0.0	14.442356496638835	0.0
8	3.2287852664070725E-4	0.0	0.0	17.46240079557269	0.0
9	3.2287852664070725E-4	0.0	0.0	18.55324589782832	0.0
10	3.2287852664070725E-4	0.0	0.0	22.988789657555035	0.0
11	3.2287852664070725E-4	0.0	0.0	27.86264101719651	0.0
12	3.2287852664070725E-4	0.0	0.0	32.56149221539872	0.0
13	3.2287852664070725E-4	0.0	0.0	33.97117986271205	0.0
14	3.2287852664070725E-4	0.0	0.0	34.55494423887845	0.0
15	4.8431778996106087E-4	0.0	0.0	35.825471241209634	0.0
16	4.8431778996106087E-4	0.0	0.0	38.05478602840039	0.0
17	4.8431778996106087E-4	0.0	0.0	41.017035071065564	0.0
18	4.8431778996106087E-4	0.0	0.0	43.901470388810324	0.0
19	4.8431778996106087E-4	0.0	0.0	46.166947570984846	0.0
20	4.8431778996106087E-4	0.0	0.0	47.887082921663215	0.0
21	4.8431778996106087E-4	0.0	0.0	49.78060404114764	0.0
22	4.8431778996106087E-4	0.0	0.0	51.62569338163596	0.0
23	4.8431778996106087E-4	0.0	0.0	53.33355934830198	0.0
24	0.002905906739766365	0.0	0.0	54.58245348934824	0.0
25	0.002905906739766365	0.0	0.0	55.522191441136016	0.0
26	0.002905906739766365	0.0	0.0	56.32309162646829	0.0
27	0.002905906739766365	0.0	0.0	57.02389946854195	0.0
28	0.002905906739766365	0.0	0.0	57.68289454141563	0.0
29	0.002905906739766365	0.0	0.0	58.37482322400666	0.0
30	0.002905906739766365	0.0	0.0	59.05399820479539	0.0
31	0.002905906739766365	0.0	0.0	59.568828015524	0.0
32	0.002905906739766365	0.0	0.0	60.08850100415222	0.0
33	0.002905906739766365	0.0	0.0	60.54246821260905	0.0
34	0.002905906739766365	0.0	0.0	60.996112542539244	0.0
35	0.002905906739766365	0.0	0.0	61.42021348728181	0.0
36	0.002905906739766365	0.0	0.0	61.83382087990856	0.0
37	0.002905906739766365	0.0	0.0	62.218207765874325	0.0
38	0.002905906739766365	0.0	0.0	62.575957173392226	0.0
39	0.002905906739766365	0.0	0.0	62.932899384593526	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGG	40	6.8066583E-9	45.000004	3
CCTTCGA	25	3.8887145E-5	45.000004	41
CTTCGAA	20	7.0307637E-4	45.000004	42
AACGTAG	30	2.1638753E-6	45.000004	1
CTCACGG	20	7.0307637E-4	45.000004	2
TGCCCGT	20	7.0307637E-4	45.000004	31
ACCGGAC	20	7.0307637E-4	45.000004	4
GCGACCG	20	7.0307637E-4	45.000004	33
GACGTTG	25	3.8887145E-5	45.000004	1
GTCTACG	20	7.0307637E-4	45.000004	1
CGATTGA	20	7.0307637E-4	45.000004	10
CTAAGCG	20	7.0307637E-4	45.000004	1
ACGGGTA	30	2.1638753E-6	45.000004	5
TCGATAG	25	3.8887145E-5	45.000004	1
ACGGGGT	160	0.0	45.000004	5
TGTTGCG	40	6.8066583E-9	45.000004	1
TACGCAG	60	0.0	45.000004	1
GCTTACG	30	2.1638753E-6	45.000004	1
CGGGCTC	20	7.0307637E-4	45.000004	6
TCGCTAA	25	3.8887145E-5	45.000004	20
>>END_MODULE