SRR2935237.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935237.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	446583
Sequences flagged as poor quality	0
Sequence length	51
%GC	41

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12912	2.891287845708413	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCC	2490	0.5575671263796428	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGC	2370	0.5306964214938769	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCG	2260	0.5060649420152581	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTC	2097	0.46956556787875936	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1973	0.4417991728301346	No Hit
GCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTGC	1866	0.41783946097366	No Hit
CCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTGC	1573	0.3522301565442482	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1054	0.23601435791331063	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1013	0.22683353374400728	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	822	0.18406432846749654	No Hit
CTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTGCT	814	0.1822729481417788	TruSeq Adapter, Index 13 (95% over 22bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	773	0.17309212397247545	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCG	753	0.16861367315818113	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	730	0.16346345472174265	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACATCACT	727	0.16279168709959851	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	670	0.1500281022788597	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGC	522	0.11688756625308173	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCT	466	0.10434790397305764	No Hit
TCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTGC	462	0.10345221381019877	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGGG	50	2.1827873E-11	45.0	3
CGTATGG	45	3.8380676E-10	45.0	2
TTTCGAC	40	6.7993824E-9	45.0	18
CGCATGT	20	7.0286496E-4	45.0	38
GACCCGA	20	7.0286496E-4	45.0	38
ATCGTAG	25	3.886962E-5	45.0	1
ACGATAG	25	3.886962E-5	45.0	1
TCTACGG	25	3.886962E-5	45.0	2
ACTACGG	20	7.0286496E-4	45.0	2
ACCACGT	20	7.0286496E-4	45.0	9
TAGTGCG	20	7.0286496E-4	45.0	1
CGAAGTG	25	3.886962E-5	45.0	37
CGTTAGG	30	2.1625092E-6	44.999996	2
CGTTATT	335	0.0	43.656715	1
CCGATGA	330	0.0	43.636364	18
CGTTTTT	4240	0.0	42.346695	1
AGGGCAT	130	0.0	41.53846	6
TGCGCGG	60	3.6379788E-12	41.249996	2
TAATACG	55	6.002665E-11	40.909092	4
CGAAAGG	45	1.9237632E-8	40.0	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content