##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935237.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	446583
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.737076870369002	31.0	31.0	34.0	30.0	34.0
2	31.850135361175862	33.0	31.0	34.0	30.0	34.0
3	31.84047310354402	33.0	31.0	34.0	30.0	34.0
4	35.61544214625277	37.0	35.0	37.0	33.0	37.0
5	33.146774507762274	37.0	35.0	37.0	28.0	37.0
6	34.11198142338602	37.0	35.0	37.0	28.0	37.0
7	35.38392863140783	37.0	35.0	37.0	32.0	37.0
8	35.28908176083729	37.0	35.0	37.0	33.0	37.0
9	37.35548599028624	39.0	37.0	39.0	34.0	39.0
10	36.86751622878614	39.0	37.0	39.0	32.0	39.0
11	36.670901041911584	39.0	37.0	39.0	32.0	39.0
12	36.94072994269822	39.0	37.0	39.0	33.0	39.0
13	36.8667213037666	39.0	37.0	39.0	33.0	39.0
14	37.81876381322173	40.0	37.0	41.0	33.0	41.0
15	37.94721697870273	40.0	37.0	41.0	33.0	41.0
16	37.98836274555906	40.0	37.0	41.0	33.0	41.0
17	37.898010000380665	40.0	37.0	41.0	33.0	41.0
18	37.78021554783769	39.0	37.0	40.0	33.0	41.0
19	37.55065463754778	39.0	36.0	40.0	33.0	41.0
20	37.246050566188146	39.0	35.0	40.0	32.0	41.0
21	37.20298578315789	39.0	35.0	40.0	33.0	41.0
22	37.216150189326505	39.0	35.0	40.0	33.0	41.0
23	36.951390894861646	39.0	35.0	40.0	32.0	41.0
24	36.66878945235264	38.0	35.0	40.0	31.0	41.0
25	36.49544653513457	38.0	35.0	40.0	31.0	41.0
26	36.72766092753195	38.0	35.0	40.0	32.0	41.0
27	36.57275131386551	38.0	35.0	40.0	31.0	41.0
28	36.607665316413744	38.0	35.0	40.0	31.0	41.0
29	36.63267074653536	39.0	35.0	40.0	31.0	41.0
30	36.32536169088389	38.0	35.0	40.0	31.0	41.0
31	36.190443881652456	38.0	35.0	40.0	30.0	41.0
32	35.83310605195451	38.0	35.0	40.0	29.0	41.0
33	35.61389260227102	38.0	35.0	40.0	27.0	41.0
34	35.393895423695035	38.0	35.0	40.0	25.0	41.0
35	35.090737891948415	38.0	35.0	40.0	24.0	41.0
36	34.920404493677545	38.0	35.0	40.0	23.0	41.0
37	34.81733742663738	38.0	35.0	40.0	22.0	41.0
38	34.730388304077856	38.0	34.0	40.0	22.0	41.0
39	34.66041922778073	38.0	34.0	40.0	21.0	41.0
40	34.53670426326125	38.0	34.0	40.0	21.0	41.0
41	34.411932384349605	38.0	34.0	40.0	20.0	41.0
42	34.44333752068484	38.0	34.0	40.0	20.0	41.0
43	34.337552034000396	38.0	34.0	40.0	19.0	41.0
44	34.27893135206669	38.0	34.0	40.0	18.0	41.0
45	34.256514466515746	38.0	34.0	40.0	20.0	41.0
46	34.12262446174619	38.0	34.0	40.0	20.0	41.0
47	33.98819480365352	37.0	33.0	40.0	20.0	41.0
48	33.94795368386168	37.0	33.0	40.0	20.0	41.0
49	33.990129494405295	37.0	33.0	40.0	20.0	41.0
50	33.89776368558588	37.0	33.0	40.0	18.0	41.0
51	32.929088209806466	36.0	32.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	10.0
10	17.0
11	16.0
12	21.0
13	21.0
14	46.0
15	59.0
16	98.0
17	141.0
18	273.0
19	530.0
20	924.0
21	1620.0
22	2560.0
23	3516.0
24	4769.0
25	6445.0
26	8082.0
27	8929.0
28	9136.0
29	9196.0
30	9665.0
31	11302.0
32	13857.0
33	19322.0
34	28197.0
35	37628.0
36	46976.0
37	60831.0
38	86648.0
39	75729.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.04440160059832	20.99497741741177	22.47174657342532	19.488874408564588
2	38.00144653961301	24.88518371724853	22.499960813555376	14.61340892958308
3	26.26880109632476	25.629950087665677	34.97804439488292	13.123204421126644
4	23.443346477586473	24.167959819339295	37.60219264951868	14.786501053555554
5	21.046031756694724	31.382520158626726	32.15012662819677	15.421321456481774
6	20.39665638862205	34.16274242414064	33.59196386785883	11.84863731937848
7	64.27808492486278	7.396161519807068	23.650026982666155	4.675726572663984
8	62.78161058526634	9.162910366046177	22.14168474841183	5.913794300275649
9	57.734396517556654	8.794781709111184	23.903731221295928	9.56709055203624
10	32.710380825064995	23.278987332701874	30.285075786583903	13.72555605564923
11	22.971093839219137	22.54384963153546	35.61465617813486	18.870400351110543
12	20.458011164777883	19.693315688237124	40.622235956138056	19.226437190846944
13	21.097757863599824	21.253607951937266	42.854967609604486	14.793666574858424
14	16.748734277838608	24.251482927025883	41.09426467196467	17.905518123170832
15	14.239906131670931	23.053273411661436	43.7361028073169	18.97071764935074
16	16.558624040771814	23.826254022208637	38.598871878239876	21.01625005877967
17	17.04923832747776	24.93175960571719	37.33975543180103	20.67924663500402
18	16.80314745523229	23.268686895828992	40.71919441626753	19.208971232671193
19	16.833600920769488	24.783746806304762	38.60626132208346	19.776390950842284
20	17.262412586238167	26.4824232001666	39.18666854761601	17.068495665979224
21	16.755228031519337	26.332171175347025	39.95584247497106	16.95675831816258
22	15.600907334134975	23.082607264495067	39.12016355302374	22.19632184834622
23	15.414155935178902	24.713659051061057	39.2930317544555	20.57915325930454
24	16.62915964109695	24.52018997588354	37.604879720007254	21.24577066301225
25	15.676369230355835	27.43454184328557	35.23130078843126	21.657788137927326
26	15.841176220321868	25.292946663890024	37.840670155379854	21.025206960408255
27	17.323767362394	25.938067503689123	37.42171108170262	19.316454052214258
28	14.13152762196501	24.965124064283685	37.706540553491735	23.19680776025957
29	17.76735791554985	24.39233020513544	36.52691660900661	21.313395270308096
30	18.3417192324831	27.810955634227007	34.886907920812035	18.96041721247786
31	17.02415900291771	28.236184539044253	31.385431151656018	23.354225306382016
32	18.345301993134537	30.04883750612988	30.57841431492018	21.0274461858154
33	17.750115879914823	26.62730108400902	31.95441832761211	23.668164708464047
34	17.858942234702173	24.965124064283685	31.462684428202596	25.71324927281155
35	18.20042410929211	29.39498368724291	29.888508966978144	22.516083236486832
36	18.788892546290388	28.523477158781237	29.708699166784225	22.978931128144154
37	16.275362026767702	29.559566754668225	30.660593887362488	23.50447733120159
38	16.354406683639997	29.454323160532308	30.034954308605567	24.156315847222128
39	18.174896939650637	26.129297353459492	31.635552629634354	24.060253077255517
40	19.197999028176174	25.052229932621707	32.931392372750416	22.8183786664517
41	17.682491272618975	27.718251702371116	28.257009335330725	26.342247689679187
42	18.262226730529374	26.825472532541543	30.880485822344333	24.03181491458475
43	18.966239198536442	25.402444786299522	31.456638519603295	24.174677495560736
44	18.52802278635774	24.614237442983722	31.447457695433993	25.41028207522454
45	19.287791967002775	23.246070719216807	31.241896803057884	26.22424051072253
46	20.69201021982476	25.230024429949193	31.683919898428737	22.394045451797314
47	15.671219011919396	26.420620578929338	34.99551035305867	22.912650056092595
48	16.459426355235195	25.71526457567798	33.15531491346513	24.669994155621687
49	19.27704368504847	22.500408658636808	34.97289417644648	23.249653479868243
50	18.346645528378826	21.721829984571738	33.801107520886376	26.130416966163068
51	17.199042507215903	22.186021411473345	31.133070448270534	29.481865633040215
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3122.0
1	4705.5
2	6289.0
3	13249.0
4	20209.0
5	12941.5
6	5674.0
7	5459.5
8	5245.0
9	5636.0
10	6027.0
11	5919.0
12	5811.0
13	5453.0
14	5095.0
15	4900.5
16	4706.0
17	4543.5
18	4381.0
19	4030.5
20	3680.0
21	3783.5
22	3887.0
23	4376.5
24	4866.0
25	4716.5
26	4866.5
27	5166.0
28	5529.0
29	5892.0
30	5822.0
31	5752.0
32	6705.0
33	7658.0
34	8347.0
35	9036.0
36	9618.0
37	10200.0
38	10586.0
39	10972.0
40	12322.5
41	13673.0
42	17039.5
43	20406.0
44	22772.5
45	25139.0
46	34200.5
47	43262.0
48	44721.0
49	46180.0
50	45634.5
51	45089.0
52	37273.5
53	29458.0
54	24401.5
55	19345.0
56	17109.0
57	14873.0
58	13487.5
59	12102.0
60	11282.5
61	10463.0
62	9333.0
63	8203.0
64	7168.5
65	6134.0
66	5335.0
67	4536.0
68	3919.0
69	3302.0
70	2887.0
71	2472.0
72	2110.5
73	1749.0
74	1325.0
75	694.5
76	488.0
77	389.0
78	290.0
79	209.5
80	129.0
81	104.5
82	80.0
83	53.5
84	27.0
85	26.5
86	26.0
87	19.0
88	12.0
89	6.5
90	1.0
91	2.5
92	4.0
93	2.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	446583.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.897855496163395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.36381614373812	31.900482390773412
2	9.98344786535952	8.565370077633471
3	4.073741771124263	5.2426435747912015
4	2.4324057717602243	4.173799652200156
5	1.6817737189458055	3.607224298629124
6	1.3200587815193738	3.3976614513635774
7	1.0359883161607497	3.1109173957664233
8	0.8485735970890443	2.9121590036628326
9	0.7384714818321252	2.851095862410494
>10	3.4115706799967187	21.489751949579563
>50	0.06704896585377505	2.0053901914971335
>100	0.03352448292688689	2.6582548412047564
>500	0.004257077197065001	1.3265022655423648
>1k	0.004789211846698127	3.8112685984332
>5k	0.0	0.0
>10k+	5.321346496331252E-4	2.947478446512307
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12912	2.891287845708413	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCC	2490	0.5575671263796428	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGC	2370	0.5306964214938769	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCG	2260	0.5060649420152581	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTC	2097	0.46956556787875936	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1973	0.4417991728301346	No Hit
GCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTGC	1866	0.41783946097366	No Hit
CCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTGC	1573	0.3522301565442482	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1054	0.23601435791331063	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1013	0.22683353374400728	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	822	0.18406432846749654	No Hit
CTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTGCT	814	0.1822729481417788	TruSeq Adapter, Index 13 (95% over 22bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	773	0.17309212397247545	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCG	753	0.16861367315818113	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	730	0.16346345472174265	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACATCACT	727	0.16279168709959851	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	670	0.1500281022788597	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGC	522	0.11688756625308173	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCT	466	0.10434790397305764	No Hit
TCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTGC	462	0.10345221381019877	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.956901628588639E-4	0.0	0.0	0.19749968091037948	0.0
2	8.956901628588639E-4	0.0	0.0	1.2120927128887575	0.0
3	8.956901628588639E-4	0.0	0.0	1.6671033156210604	0.0
4	8.956901628588639E-4	0.0	0.0	2.327226965648043	0.0
5	8.956901628588639E-4	0.0	0.0	4.496140695010782	0.0
6	8.956901628588639E-4	0.0	0.0	5.628516983405101	0.0
7	8.956901628588639E-4	0.0	0.0	6.907338613426844	0.0
8	8.956901628588639E-4	0.0	0.0	8.726261411652482	0.0
9	8.956901628588639E-4	0.0	0.0	9.394446273145194	0.0
10	8.956901628588639E-4	0.0	0.0	11.590678552475127	0.0
11	8.956901628588639E-4	0.0	0.0	14.664911113947463	0.0
12	8.956901628588639E-4	0.0	0.0	17.324439130016145	0.0
13	8.956901628588639E-4	0.0	0.0	18.16549219294062	0.0
14	8.956901628588639E-4	0.0	0.0	18.531381624468462	0.0
15	8.956901628588639E-4	0.0	0.0	19.272789156774888	0.0
16	8.956901628588639E-4	0.0	0.0	20.669394043212574	0.0
17	8.956901628588639E-4	0.0	0.0	22.29551953388284	0.0
18	8.956901628588639E-4	0.0	0.0	23.989493554389664	0.0
19	8.956901628588639E-4	0.0	0.0	25.257790824997816	0.0
20	8.956901628588639E-4	0.0	0.0	26.31918366798557	0.0
21	8.956901628588639E-4	0.0	0.0	27.528141465304323	0.0
22	8.956901628588639E-4	0.0	0.0	28.804052102296772	0.0
23	8.956901628588639E-4	0.0	0.0	29.848202909649494	0.0
24	8.956901628588639E-4	0.0	0.0	30.595656350555217	0.0
25	8.956901628588639E-4	0.0	0.0	31.217713168660698	0.0
26	8.956901628588639E-4	0.0	0.0	31.77953482331392	0.0
27	8.956901628588639E-4	0.0	0.0	32.34964161197359	0.0
28	8.956901628588639E-4	0.0	0.0	32.83936020851667	0.0
29	8.956901628588639E-4	0.0	0.0	33.32571996694903	0.0
30	8.956901628588639E-4	0.0	0.0	33.820140936847125	0.0
31	8.956901628588639E-4	0.0	0.0	34.21245322817931	0.0
32	8.956901628588639E-4	0.0	0.0	34.56781830029356	0.0
33	8.956901628588639E-4	0.0	0.0	34.889818913841324	0.0
34	8.956901628588639E-4	0.0	0.0	35.249662436769874	0.0
35	8.956901628588639E-4	0.0	0.0	35.63033075598489	0.0
36	8.956901628588639E-4	0.0	0.0	35.93777640438619	0.0
37	8.956901628588639E-4	0.0	0.0	36.26850999702183	0.0
38	8.956901628588639E-4	0.0	0.0	36.6120071744782	0.0
39	8.956901628588639E-4	0.0	0.0	37.02021796620113	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGGG	50	2.1827873E-11	45.0	3
CGTATGG	45	3.8380676E-10	45.0	2
TTTCGAC	40	6.7993824E-9	45.0	18
CGCATGT	20	7.0286496E-4	45.0	38
GACCCGA	20	7.0286496E-4	45.0	38
ATCGTAG	25	3.886962E-5	45.0	1
ACGATAG	25	3.886962E-5	45.0	1
TCTACGG	25	3.886962E-5	45.0	2
ACTACGG	20	7.0286496E-4	45.0	2
ACCACGT	20	7.0286496E-4	45.0	9
TAGTGCG	20	7.0286496E-4	45.0	1
CGAAGTG	25	3.886962E-5	45.0	37
CGTTAGG	30	2.1625092E-6	44.999996	2
CGTTATT	335	0.0	43.656715	1
CCGATGA	330	0.0	43.636364	18
CGTTTTT	4240	0.0	42.346695	1
AGGGCAT	130	0.0	41.53846	6
TGCGCGG	60	3.6379788E-12	41.249996	2
TAATACG	55	6.002665E-11	40.909092	4
CGAAAGG	45	1.9237632E-8	40.0	2
>>END_MODULE