SRR2935234.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935234.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	562405
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11609	2.064170837741485	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCG	10340	1.838532729972173	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTC	10107	1.7971035108151598	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGC	7324	1.3022643824290323	No Hit
GCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	7143	1.2700811692641425	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	3748	0.6664236626630275	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCC	3484	0.6194824014722486	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3206	0.5700518309758982	No Hit
CTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGCT	3045	0.5414247739618246	Illumina Single End Adapter 1 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCT	2645	0.47030165094549303	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCTCTAGAT	2245	0.39917852792916136	No Hit
GAACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCT	2065	0.3671731225718121	No Hit
TCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	1939	0.34476933882166766	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTC	1749	0.3109858553889101	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTA	1538	0.2734684079977952	No Hit
ACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	1294	0.23008330295783289	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCG	1140	0.2027009005965452	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1061	0.18865408380081972	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1032	0.18349765738213564	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGC	914	0.1625163360923178	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	838	0.1490029427192148	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCG	788	0.14011255234217335	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	771	0.13708981961397926	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	761	0.13531174153857095	No Hit
ACCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTG	760	0.1351339337310301	No Hit
GCCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTG	740	0.13157777758021355	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTC	704	0.1251766965087437	No Hit
AGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTG	695	0.12357642624087624	No Hit
CGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTG	680	0.1209093091277638	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTT	633	0.11255234217334484	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGT	579	0.10295072056614005	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	564	0.10028360345302761	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	25	3.888256E-5	45.0	21
TCACGAC	20	7.0302107E-4	45.0	25
CGGGTAT	40	6.8048394E-9	45.0	6
TAGACGG	20	7.0302107E-4	45.0	2
GACACGA	25	3.888256E-5	45.0	25
GCGCCCA	20	7.0302107E-4	45.0	33
ACGGGTA	40	6.8048394E-9	45.0	5
ACGGGCG	20	7.0302107E-4	45.0	5
GTCAGGC	20	7.0302107E-4	45.0	3
ATCCGGC	35	1.2104465E-7	45.0	6
AACGGGC	25	3.888256E-5	45.0	4
ATCCATA	20	7.0302107E-4	45.0	20
TGTTACG	20	7.0302107E-4	45.0	1
ATGACGG	25	3.888256E-5	45.0	2
CATAAGG	45	3.8380676E-10	45.0	2
TTTGCGG	35	1.2104465E-7	45.0	2
CGCACGG	25	3.888256E-5	45.0	2
TGATGCG	20	7.0302107E-4	45.0	1
GCGTAAG	25	3.888256E-5	45.0	1
ATCGAAG	20	7.0302107E-4	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content