##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935234.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	562405
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.475283825712786	31.0	31.0	34.0	30.0	34.0
2	31.55668068384883	31.0	31.0	34.0	30.0	34.0
3	31.599130519821124	31.0	31.0	34.0	30.0	34.0
4	35.46108765035873	37.0	35.0	37.0	33.0	37.0
5	32.898587316969085	35.0	35.0	37.0	28.0	37.0
6	33.905146646989266	36.0	35.0	37.0	26.0	37.0
7	35.20139401321112	37.0	35.0	37.0	32.0	37.0
8	34.97167343818067	37.0	35.0	37.0	32.0	37.0
9	36.98717827899823	39.0	37.0	39.0	33.0	39.0
10	36.673717338928356	39.0	35.0	39.0	32.0	39.0
11	36.533281176376455	39.0	35.0	39.0	32.0	39.0
12	36.8510468434669	39.0	37.0	39.0	33.0	39.0
13	36.74205776975667	39.0	35.0	39.0	33.0	39.0
14	37.548942488064654	39.0	36.0	41.0	32.0	41.0
15	37.662526115521736	39.0	37.0	41.0	32.0	41.0
16	37.72344307038522	39.0	37.0	41.0	33.0	41.0
17	37.61201269547746	39.0	36.0	41.0	32.0	41.0
18	37.546001546927926	39.0	36.0	40.0	33.0	41.0
19	37.384167992816565	39.0	36.0	40.0	32.0	41.0
20	37.068011486384364	39.0	35.0	40.0	32.0	41.0
21	37.08900703229879	39.0	35.0	40.0	32.0	41.0
22	37.127143250860144	39.0	35.0	40.0	32.0	41.0
23	36.778846205136865	39.0	35.0	40.0	31.0	41.0
24	36.51890541513678	38.0	35.0	40.0	31.0	41.0
25	36.32254691903521	38.0	35.0	40.0	30.0	41.0
26	36.680650065344366	38.0	35.0	40.0	31.0	41.0
27	36.51687129381851	38.0	35.0	40.0	31.0	41.0
28	36.65756705576942	39.0	35.0	40.0	31.0	41.0
29	36.804880824316996	39.0	35.0	40.0	32.0	41.0
30	36.535598012108714	39.0	35.0	40.0	31.0	41.0
31	36.40937224953548	39.0	35.0	40.0	30.0	41.0
32	36.11689796498964	39.0	35.0	40.0	30.0	41.0
33	35.952856037908624	39.0	35.0	40.0	29.0	41.0
34	35.7639494670211	39.0	35.0	40.0	27.0	41.0
35	35.64021123567536	39.0	35.0	40.0	27.0	41.0
36	35.451370453676624	39.0	35.0	40.0	25.0	41.0
37	35.45469545967764	39.0	35.0	40.0	25.0	41.0
38	35.37519758892613	39.0	35.0	40.0	25.0	41.0
39	35.24988931463981	38.0	35.0	40.0	24.0	41.0
40	35.126245321432066	38.0	35.0	40.0	24.0	41.0
41	34.826888096656326	38.0	34.0	40.0	23.0	41.0
42	35.040965140779335	38.0	35.0	40.0	23.0	41.0
43	34.99104026457802	38.0	35.0	40.0	23.0	41.0
44	35.01406104142033	38.0	35.0	40.0	23.0	41.0
45	35.025106462424766	38.0	35.0	40.0	23.0	41.0
46	34.887132938007305	38.0	35.0	40.0	23.0	41.0
47	34.73290066766832	38.0	34.0	40.0	23.0	41.0
48	34.70057343017932	38.0	34.0	40.0	22.0	41.0
49	34.771760564006364	38.0	34.0	40.0	23.0	41.0
50	34.678959113094656	38.0	34.0	40.0	22.0	41.0
51	33.66159795876637	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	6.0
10	17.0
11	15.0
12	21.0
13	21.0
14	36.0
15	58.0
16	94.0
17	176.0
18	292.0
19	542.0
20	1102.0
21	1911.0
22	3023.0
23	4219.0
24	5603.0
25	7105.0
26	8638.0
27	9494.0
28	9557.0
29	10237.0
30	11664.0
31	14129.0
32	18499.0
33	24728.0
34	35675.0
35	47036.0
36	60884.0
37	80868.0
38	119227.0
39	87508.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.377628221655215	19.883002462638135	19.61220117175345	16.127168143953202
2	33.66719712662583	30.55271556974067	20.536801770965763	15.243285532667738
3	24.570905308452094	31.083649683057583	31.265013646749228	13.080431361741093
4	22.5728789751158	21.888496723891144	40.15558183159823	15.38304246939483
5	22.643646482517045	29.260408424533924	30.597523137240955	17.498421955708075
6	18.30140201456246	34.59695415225683	33.86563063984139	13.23601319333932
7	56.620584809879006	7.931650678781306	29.0708653016954	6.3768992096442965
8	51.540793556245056	13.572603373014108	24.777517980814537	10.1090850899263
9	46.59649185195722	9.310728034067976	26.733581671571198	17.359198442403606
10	28.979827704234495	22.223486633298066	33.68640036984024	15.110285292627198
11	20.92335594455953	21.86982690409936	36.4150389843618	20.791778166979313
12	18.38550510752927	18.322383335852276	42.02949831527103	21.262613241347427
13	20.13300024004054	21.393301979889937	44.75742569856243	13.7162720815071
14	14.8233034912563	27.281585334412036	37.74201865203901	20.153092522292653
15	11.12579013344476	24.083711915790225	45.46403392572968	19.32646402503534
16	12.64604688791885	26.31022128181649	35.27386847556476	25.769863354699908
17	12.844658208941956	27.46775010890728	37.13107102532872	22.55652065682204
18	12.77051235319743	24.998533085587788	41.192556965176344	21.03839759603844
19	12.341462113601407	26.91032263226678	36.469448173469296	24.27876708066251
20	13.571892141783945	29.052906713133776	40.485059698971384	16.8901414461109
21	14.101759408255615	30.580275779909492	38.67942141339426	16.638543398440625
22	11.249722175300718	25.351481583556335	37.12218063495168	26.276615606191267
23	12.640179230270002	27.19072554475867	37.878752856037906	22.29034236893342
24	17.253936220339437	24.28765747103955	33.817978147420455	24.64042816120056
25	11.802704456752695	31.867248690890015	31.766965087436986	24.563081764920298
26	12.071194246139347	24.511161885118376	36.93708270730168	26.480561161440598
27	16.9201909655853	24.88704759025969	33.268729829926826	24.92403161422818
28	9.455819205021292	24.508139152390182	37.912002916048046	28.124038726540483
29	14.093046825686114	20.52808918839626	33.34340910909398	32.03545487682364
30	14.542722770956873	26.117299810634687	34.00343169068554	25.336545727722903
31	13.543976316000036	27.541895964651808	31.80768307536384	27.106444643984318
32	17.553897991660815	25.97700945048497	27.70352326170642	28.765569296147792
33	14.548234812990637	25.071434286679526	32.29718796952375	28.083142930806094
34	14.60833385193944	25.683804375850144	31.615828451027284	28.092033321183134
35	17.28931997404006	23.51045954427859	26.633298068118172	32.566922413563184
36	14.878068295978878	28.719339266187177	30.749371004880828	25.65322143295312
37	14.065308807709748	25.58938843004596	33.83629235159716	26.509010410647132
38	15.877170366550795	25.323565757772425	32.163298690445494	26.635965185231285
39	18.05869435726923	26.492829900160913	31.790435718032377	23.658040024537478
40	20.788222010828495	23.270774619713553	33.592873463073765	22.34812990638419
41	18.258728140752662	30.938558512104265	25.16051599825748	25.64219734888559
42	20.03449471466292	23.75156693130395	32.374534365804	23.839403988229122
43	20.04676345338324	23.302246601648278	30.66473448849139	25.986255456477092
44	17.72690498839804	20.500706786034975	31.907077639779164	29.865310585787824
45	18.70467012206506	17.746641655035074	31.337381424418346	32.21130679848152
46	23.56486873338608	22.42565411047199	30.923978271885915	23.08549888425601
47	13.778860429761473	22.837456992736552	40.54853708626346	22.83514549123852
48	17.230287781936504	22.955699184751204	32.49882202327504	27.31519101003725
49	19.087312523892923	18.26103964225069	39.54552324392564	23.106124589930744
50	19.136920902196817	18.059761204114473	34.94296814573128	27.86034974795743
51	17.68351988335808	18.180314897627156	29.783341186511496	34.352824032503264
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4339.0
1	5415.0
2	6491.0
3	12615.5
4	18740.0
5	12202.5
6	5665.0
7	5723.0
8	5781.0
9	5789.5
10	5798.0
11	6046.5
12	6295.0
13	5952.0
14	5609.0
15	5365.5
16	5122.0
17	5107.0
18	5092.0
19	5204.5
20	5317.0
21	5211.0
22	5105.0
23	4765.0
24	4425.0
25	4754.0
26	4976.5
27	4870.0
28	5032.5
29	5195.0
30	5865.0
31	6535.0
32	6994.5
33	7454.0
34	8179.5
35	8905.0
36	9051.0
37	9197.0
38	9954.5
39	10712.0
40	12772.0
41	14832.0
42	19239.0
43	23646.0
44	29276.5
45	34907.0
46	61507.5
47	88108.0
48	83369.0
49	78630.0
50	76260.5
51	73891.0
52	56504.0
53	39117.0
54	30593.5
55	22070.0
56	17781.5
57	13493.0
58	11399.5
59	9306.0
60	8015.5
61	6725.0
62	5968.5
63	5212.0
64	4427.0
65	3642.0
66	3157.5
67	2673.0
68	2173.5
69	1674.0
70	1366.0
71	1058.0
72	924.5
73	791.0
74	621.0
75	322.5
76	194.0
77	164.5
78	135.0
79	99.5
80	64.0
81	45.5
82	27.0
83	20.0
84	13.0
85	9.5
86	6.0
87	7.0
88	8.0
89	4.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	562405.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.73591224217561
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.53708826990906	27.372531972562342
2	11.100903080539421	8.378054089122648
3	4.8330686440928305	5.471407626416931
4	2.68174159585142	4.047918620689646
5	1.8731177927006963	3.5341904322305577
6	1.4207253767878611	3.216742088321916
7	1.0458248253733666	2.762560768168459
8	0.8330094948761609	2.514749855643667
9	0.6683481683171532	2.269865504415141
>10	2.824201305870247	16.90677064196903
>50	0.09456783791366737	2.4980225427057476
>100	0.0716423014492948	5.404632178049216
>500	0.006686614801934181	1.7961971616513317
>1k	0.006686614801934181	5.4413996094135415
>5k	9.552306859905974E-4	2.6074236742534436
>10k+	0.0014328460289858961	5.777533234386423
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11609	2.064170837741485	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCG	10340	1.838532729972173	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTC	10107	1.7971035108151598	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGC	7324	1.3022643824290323	No Hit
GCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	7143	1.2700811692641425	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	3748	0.6664236626630275	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCC	3484	0.6194824014722486	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3206	0.5700518309758982	No Hit
CTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGCT	3045	0.5414247739618246	Illumina Single End Adapter 1 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCT	2645	0.47030165094549303	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCTCTAGAT	2245	0.39917852792916136	No Hit
GAACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCT	2065	0.3671731225718121	No Hit
TCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	1939	0.34476933882166766	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTC	1749	0.3109858553889101	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTA	1538	0.2734684079977952	No Hit
ACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	1294	0.23008330295783289	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCG	1140	0.2027009005965452	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1061	0.18865408380081972	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1032	0.18349765738213564	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGC	914	0.1625163360923178	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	838	0.1490029427192148	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCG	788	0.14011255234217335	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	771	0.13708981961397926	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	761	0.13531174153857095	No Hit
ACCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTG	760	0.1351339337310301	No Hit
GCCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTG	740	0.13157777758021355	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTC	704	0.1251766965087437	No Hit
AGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTG	695	0.12357642624087624	No Hit
CGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTG	680	0.1209093091277638	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTT	633	0.11255234217334484	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGT	579	0.10295072056614005	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	564	0.10028360345302761	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.5561561508165823E-4	0.0	0.0	0.5748526417795006	0.0
2	3.5561561508165823E-4	0.0	0.0	3.259928343453561	0.0
3	3.5561561508165823E-4	0.0	0.0	4.220623927596661	0.0
4	3.5561561508165823E-4	0.0	0.0	5.8288955468034604	0.0
5	3.5561561508165823E-4	0.0	0.0	10.967541184733422	0.0
6	3.5561561508165823E-4	0.0	0.0	12.927872262871063	0.0
7	3.5561561508165823E-4	0.0	0.0	15.370240307251892	0.0
8	3.5561561508165823E-4	0.0	0.0	18.37803717961256	0.0
9	3.5561561508165823E-4	0.0	0.0	19.33162045145402	0.0
10	3.5561561508165823E-4	0.0	0.0	23.98218365768441	0.0
11	3.5561561508165823E-4	0.0	0.0	28.27802028787084	0.0
12	3.5561561508165823E-4	0.0	0.0	32.8466140948249	0.0
13	3.5561561508165823E-4	0.0	0.0	34.156879828593276	0.0
14	3.5561561508165823E-4	0.0	0.0	34.7253313893013	0.0
15	3.5561561508165823E-4	0.0	0.0	36.08467207795094	0.0
16	3.5561561508165823E-4	0.0	0.0	38.00552982281452	0.0
17	3.5561561508165823E-4	0.0	0.0	40.16607249224313	0.0
18	3.5561561508165823E-4	0.0	0.0	42.29727687342751	0.0
19	3.5561561508165823E-4	0.0	0.0	44.21013326695175	0.0
20	3.5561561508165823E-4	0.0	0.0	45.75279380517598	0.0
21	3.5561561508165823E-4	0.0	0.0	47.377779358291626	0.0
22	3.5561561508165823E-4	0.0	0.0	49.0660644908918	0.0
23	3.5561561508165823E-4	0.0	0.0	50.40655755194211	0.0
24	3.5561561508165823E-4	0.0	0.0	51.38716761052978	0.0
25	3.5561561508165823E-4	0.0	0.0	52.149251873649774	0.0
26	3.5561561508165823E-4	0.0	0.0	52.79949502582659	0.0
27	3.5561561508165823E-4	0.0	0.0	53.4301793191739	0.0
28	3.5561561508165823E-4	0.0	0.0	53.94208799708395	0.0
29	3.5561561508165823E-4	0.0	0.0	54.44030547381335	0.0
30	3.5561561508165823E-4	0.0	0.0	54.97728505258666	0.0
31	3.5561561508165823E-4	0.0	0.0	55.45256532214329	0.0
32	3.5561561508165823E-4	0.0	0.0	55.816893519794455	0.0
33	3.5561561508165823E-4	0.0	0.0	56.18175514086823	0.0
34	3.5561561508165823E-4	0.0	0.0	56.535059254451866	0.0
35	3.5561561508165823E-4	0.0	0.0	56.88427378846205	0.0
36	3.5561561508165823E-4	0.0	0.0	57.202549763960135	0.0
37	3.5561561508165823E-4	0.0	0.0	57.48864252629333	0.0
38	3.5561561508165823E-4	0.0	0.0	57.803006730025515	0.0
39	3.5561561508165823E-4	0.0	0.0	58.16217850125799	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	25	3.888256E-5	45.0	21
TCACGAC	20	7.0302107E-4	45.0	25
CGGGTAT	40	6.8048394E-9	45.0	6
TAGACGG	20	7.0302107E-4	45.0	2
GACACGA	25	3.888256E-5	45.0	25
GCGCCCA	20	7.0302107E-4	45.0	33
ACGGGTA	40	6.8048394E-9	45.0	5
ACGGGCG	20	7.0302107E-4	45.0	5
GTCAGGC	20	7.0302107E-4	45.0	3
ATCCGGC	35	1.2104465E-7	45.0	6
AACGGGC	25	3.888256E-5	45.0	4
ATCCATA	20	7.0302107E-4	45.0	20
TGTTACG	20	7.0302107E-4	45.0	1
ATGACGG	25	3.888256E-5	45.0	2
CATAAGG	45	3.8380676E-10	45.0	2
TTTGCGG	35	1.2104465E-7	45.0	2
CGCACGG	25	3.888256E-5	45.0	2
TGATGCG	20	7.0302107E-4	45.0	1
GCGTAAG	25	3.888256E-5	45.0	1
ATCGAAG	20	7.0302107E-4	45.0	1
>>END_MODULE