FastQCFastQC Report
Sat 14 Jan 2017
SRR2935232.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935232.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1406165
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC373042.6528892413052523RNA PCR Primer, Index 10 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCG367342.612353457809005No Hit
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC237061.6858619009860152RNA PCR Primer, Index 35 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGC209541.4901522936497495No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151441.0769717636266014No Hit
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC123370.8773508087599962RNA PCR Primer, Index 10 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT97630.6942997443401023TruSeq Adapter, Index 10 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCT89760.6383319169514247No Hit
GAACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT68380.4862871711356775TruSeq Adapter, Index 10 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC64220.4567031607243816RNA PCR Primer, Index 35 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTC57400.40820245134817035No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCTTCTCT46900.33353127122350507No Hit
ACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC43500.309352031945042TruSeq Adapter, Index 10 (95% over 24bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTA43490.30928091653539946No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCC39570.2814036759555244No Hit
ACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG30950.22010219284365634TruSeq Adapter, Index 10 (95% over 23bp)
GAATAATACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCG26830.1908026440709305No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTT26740.19016260538414767No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24520.17437498444350413No Hit
GCCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG24420.17366383034707875RNA PCR Primer, Index 35 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG23780.1691124441299563TruSeq Adapter, Index 10 (95% over 23bp)
GGGGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC21640.15389374646645307RNA PCR Primer, Index 10 (95% over 21bp)
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCCTCTTCTCTC21030.14955570647825825No Hit
CGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG19920.1416618960079365TruSeq Adapter, Index 10 (95% over 23bp)
GAATATGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC19410.13803501011616703RNA PCR Primer, Index 10 (95% over 21bp)
GACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT16570.11783823377768611RNA PCR Primer, Index 10 (95% over 22bp)
GGACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC16150.1148513865726995TruSeq Adapter, Index 10 (95% over 21bp)
AGCCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT16150.1148513865726995RNA PCR Primer, Index 10 (95% over 22bp)
GCTGACTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC15740.11193565477735544RNA PCR Primer, Index 10 (95% over 24bp)
GGCCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT14900.1059619603673822TruSeq Adapter, Index 10 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG14430.10261953611418291TruSeq Adapter, Index 10 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTA207.033824E-445.00000441
GTTTCGG207.033824E-445.0000042
TGCCACG207.033824E-445.0000041
TTCACGG406.8175723E-945.0000042
TAACGGA207.033824E-445.0000043
GTTACCG207.033824E-445.00000438
ACGATAG207.033824E-445.0000041
ATAAGCG207.033824E-445.0000041
CCGGTAT207.033824E-445.00000442
AAATGCG207.033824E-445.0000041
CCGATAT351.2121927E-745.011
GCACGCG253.891252E-545.01
TCCTCGC253.891252E-545.013
ACGATTG253.891252E-545.01
GTATGCG253.891252E-545.01
TCGTCCC750.045.038
GAACACG351.2121927E-745.01
GCGAGAC302.1658507E-644.99999621
TTGCACG302.1658507E-644.9999961
GCGTAAG302.1658507E-644.9999961
Produced by FastQC (version 0.11.5)