##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935232.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1406165
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.33288981022853	31.0	31.0	34.0	30.0	34.0
2	31.414833963297337	31.0	31.0	34.0	30.0	34.0
3	31.49169051996032	31.0	31.0	34.0	30.0	34.0
4	35.40205665764686	37.0	35.0	37.0	33.0	37.0
5	32.864043693307686	35.0	35.0	37.0	28.0	37.0
6	33.82415719350147	35.0	35.0	37.0	26.0	37.0
7	35.15981197085691	36.0	35.0	37.0	32.0	37.0
8	34.93395654137317	36.0	35.0	37.0	32.0	37.0
9	36.87164664175257	39.0	37.0	39.0	33.0	39.0
10	36.59494013860393	38.0	35.0	39.0	32.0	39.0
11	36.47755064306109	39.0	35.0	39.0	32.0	39.0
12	36.802788435212086	39.0	35.0	39.0	33.0	39.0
13	36.66196072295926	39.0	35.0	39.0	32.0	39.0
14	37.313763320805165	39.0	36.0	40.0	32.0	41.0
15	37.45807071005181	39.0	36.0	40.0	32.0	41.0
16	37.49826371727358	39.0	36.0	40.0	32.0	41.0
17	37.518129095803125	39.0	36.0	40.0	32.0	41.0
18	37.51803735692469	39.0	36.0	40.0	33.0	41.0
19	37.45280745858416	39.0	36.0	40.0	32.0	41.0
20	37.22835229151629	39.0	35.0	40.0	32.0	41.0
21	37.21831861836982	39.0	35.0	40.0	32.0	41.0
22	37.277541398057835	39.0	35.0	40.0	32.0	41.0
23	36.86569001504091	39.0	35.0	40.0	31.0	41.0
24	36.65305991828839	39.0	35.0	40.0	31.0	41.0
25	36.305851731482434	38.0	35.0	40.0	30.0	41.0
26	36.6913555663809	38.0	35.0	40.0	31.0	41.0
27	36.603636130895026	38.0	35.0	40.0	31.0	41.0
28	36.63719264808895	38.0	35.0	40.0	31.0	41.0
29	36.8834375766713	39.0	35.0	40.0	32.0	41.0
30	36.69883975209168	39.0	35.0	40.0	31.0	41.0
31	36.507735578683864	38.0	35.0	40.0	31.0	41.0
32	36.43776654944477	38.0	35.0	40.0	30.0	41.0
33	36.372783421575704	39.0	35.0	40.0	30.0	41.0
34	36.3689439006091	39.0	35.0	40.0	30.0	41.0
35	36.334533287345366	39.0	35.0	40.0	30.0	41.0
36	36.248765969854176	39.0	35.0	40.0	30.0	41.0
37	36.24782866875509	39.0	35.0	40.0	30.0	41.0
38	36.175188544729814	39.0	35.0	40.0	30.0	41.0
39	36.110932927501395	39.0	35.0	40.0	30.0	41.0
40	35.97450370333496	39.0	35.0	40.0	29.0	41.0
41	35.79289983750129	39.0	35.0	40.0	28.0	41.0
42	35.993670728541815	39.0	35.0	40.0	29.0	41.0
43	35.924437032638416	39.0	35.0	40.0	29.0	41.0
44	35.8647960943417	39.0	35.0	40.0	29.0	41.0
45	35.914635195727385	39.0	35.0	40.0	29.0	41.0
46	35.77293631970644	39.0	35.0	40.0	28.0	41.0
47	35.71786027955468	39.0	35.0	40.0	28.0	41.0
48	35.72798569157958	39.0	35.0	40.0	28.0	41.0
49	35.88334157086828	39.0	35.0	40.0	29.0	41.0
50	35.81959016189423	39.0	35.0	40.0	28.0	41.0
51	34.697033420686765	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	14.0
10	25.0
11	22.0
12	24.0
13	39.0
14	52.0
15	76.0
16	119.0
17	273.0
18	450.0
19	892.0
20	1759.0
21	3282.0
22	5120.0
23	7397.0
24	9633.0
25	12287.0
26	15055.0
27	16956.0
28	19291.0
29	22482.0
30	27825.0
31	35810.0
32	47829.0
33	66338.0
34	91112.0
35	111289.0
36	155130.0
37	224839.0
38	337225.0
39	193494.0
40	23.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.39526655833419	21.105275696664332	19.83636344241252	12.663094302588956
2	30.428505900801117	31.966661095959576	21.53445719385705	16.070375809382256
3	25.088378675333267	32.59958824177817	28.8379386487361	13.474094434152464
4	24.42117390206697	21.127534819882445	37.610735582239634	16.840555695810945
5	23.805243339152945	27.47586520785256	28.520052767633953	20.19883868536054
6	19.430578914992193	33.46413827680251	32.62113621089986	14.484146597305436
7	59.628919792485235	6.050499052387166	26.46602639092852	7.85455476419908
8	52.519867867568884	12.060817898326299	23.15681303403228	12.262501200072538
9	47.17846056472747	7.503600217613153	24.470954688816747	20.846984528842633
10	30.113322405265386	19.12954738597533	32.555141110751585	18.2019890980077
11	20.67260954439913	19.33265299591442	35.05342545149396	24.941312008192497
12	19.70188420277848	14.927906753474876	42.89269040261989	22.477518641126753
13	20.064074984087927	19.093491873286563	46.581802277826576	14.26063086479894
14	14.75929211721242	25.721519167380784	36.884931711427896	22.63425700397891
15	10.212741747945655	22.799813677626737	47.3187712679522	19.668673306475416
16	11.381381274601488	26.93339686309928	32.717782052604065	28.96743980969516
17	11.43187321544769	26.184978292021206	35.45480082351644	26.928347669014663
18	11.423694943338797	25.120167263443477	40.73447995078814	22.721657842429586
19	11.142860190660413	26.62041794526247	34.04906252111239	28.187659342964732
20	11.569908225563855	30.124629755398548	39.742419986274726	18.56304203276287
21	12.60854878339313	30.446711445669607	38.36605234805304	18.578687422884226
22	9.18896431073167	24.2908193561922	34.6398893444226	31.880326988653536
23	11.00660306578531	26.927138707050734	37.0444435752561	25.021814651907846
24	18.994001415196653	22.13175551944473	30.601671923280698	28.272571142077922
25	11.15900338864927	32.603428473898866	28.30507088428456	27.932497253167305
26	11.286442202728699	22.865951008594298	34.87720146639975	30.970405322277255
27	17.864190902205646	22.79483559895176	33.18863717984731	26.152336318995285
28	8.879754509605913	21.275952679806423	34.97093157630861	34.87336123427905
29	14.975554077935376	17.42370205487976	33.80833685947239	33.79240700771247
30	14.710933638655494	23.342068676151094	37.87464486742309	24.07235281777032
31	11.955353745826415	22.259336564343446	27.385193060558326	38.400116629271814
32	14.198262650542432	21.55906312559337	36.14113564197658	28.101538581887613
33	12.151134468572323	16.991107018024202	35.70327806480748	35.154480448596004
34	11.70282292618576	14.802601401684722	32.33987476576362	41.154700906365896
35	10.028695067790764	15.19160269242941	40.98693965501915	33.79276258476068
36	12.290165094423486	15.702069102843549	32.75319752660605	39.25456827612691
37	10.397357351377682	15.67269843866118	44.13408099334004	29.795863216621093
38	11.632276439820362	17.51444531758364	37.76747394509179	33.085804297504204
39	18.177738743319598	13.517901526492269	36.62322700394335	31.681132726244787
40	16.584113528639953	13.269993208478379	42.61270903485722	27.53318422802445
41	13.57401158470023	18.812586005198536	29.180714923213134	38.43268748688809
42	14.967660267465055	13.627845949799633	40.82700109873308	30.577492684002234
43	20.460472277435436	13.396151945184243	33.494789018358446	32.64858675902188
44	18.302972979700105	13.142198817350737	33.41613537529379	35.13869282765536
45	17.74194351303012	10.010347292102988	34.36019243829849	37.887516756568395
46	23.799838568020114	14.944618874740872	34.26276432708821	26.992778230150797
47	11.224358450110762	14.209001077398456	46.75745733964364	27.809183132847142
48	15.790821134077436	14.06172106402876	35.68116117240864	34.46629662948516
49	17.68810914793072	9.504858960363826	44.363570420256515	28.443461471448938
50	17.88246756248378	8.808141292095877	39.03958639277752	34.26980475264283
51	17.0588088880039	9.280632073760902	31.162488043721755	42.49807099451345
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3849.0
1	5932.5
2	8016.0
3	16086.0
4	24156.0
5	15328.0
6	6500.0
7	6495.5
8	6491.0
9	6883.0
10	7275.0
11	7377.0
12	7479.0
13	7340.5
14	7202.0
15	7218.5
16	7235.0
17	6445.5
18	5656.0
19	5844.0
20	6032.0
21	6688.0
22	7344.0
23	6678.5
24	6013.0
25	6843.5
26	8174.0
27	8674.0
28	9158.0
29	9642.0
30	10348.0
31	11054.0
32	12284.0
33	13514.0
34	14446.5
35	15379.0
36	16511.0
37	17643.0
38	19893.5
39	22144.0
40	27447.0
41	32750.0
42	43401.5
43	54053.0
44	74774.0
45	95495.0
46	189447.5
47	283400.0
48	266579.5
49	249759.0
50	242147.0
51	234535.0
52	175194.0
53	115853.0
54	85549.0
55	55245.0
56	41073.0
57	26901.0
58	21548.5
59	16196.0
60	13252.5
61	10309.0
62	8700.0
63	7091.0
64	6139.5
65	5188.0
66	4249.0
67	3310.0
68	2837.5
69	2365.0
70	2047.0
71	1729.0
72	1474.5
73	1220.0
74	1024.0
75	652.0
76	476.0
77	361.5
78	247.0
79	180.5
80	114.0
81	105.5
82	97.0
83	58.0
84	19.0
85	12.0
86	5.0
87	4.0
88	3.0
89	2.0
90	1.0
91	1.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1406165.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.125224856917402
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.97795104468727	17.89666757685831
2	12.653613182814244	6.864642056725813
3	6.019930685017771	4.898759203624915
4	3.5421139688718406	3.8432255149790713
5	2.3721308732020896	3.2172291662821246
6	1.6915857843182145	2.7530786858637932
7	1.2912885260932236	2.45185441378053
8	0.9812465350550871	2.1293226322752252
9	0.7899093760115441	1.92838224968104
>10	4.2714127764557785	19.985441940205988
>50	0.23933763855860996	4.539727233697509
>100	0.13645003666961159	6.716498392444545
>500	0.017868457116001282	3.403743571589277
>1k	0.012183038942728147	5.864993346165096
>5k	0.0013536709936364608	2.77144876153116
>10k+	0.0016244051923637527	10.734985254295646
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC	37304	2.6528892413052523	RNA PCR Primer, Index 10 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCG	36734	2.612353457809005	No Hit
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	23706	1.6858619009860152	RNA PCR Primer, Index 35 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGC	20954	1.4901522936497495	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15144	1.0769717636266014	No Hit
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	12337	0.8773508087599962	RNA PCR Primer, Index 10 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT	9763	0.6942997443401023	TruSeq Adapter, Index 10 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCT	8976	0.6383319169514247	No Hit
GAACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT	6838	0.4862871711356775	TruSeq Adapter, Index 10 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	6422	0.4567031607243816	RNA PCR Primer, Index 35 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTC	5740	0.40820245134817035	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCTTCTCT	4690	0.33353127122350507	No Hit
ACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	4350	0.309352031945042	TruSeq Adapter, Index 10 (95% over 24bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTA	4349	0.30928091653539946	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCC	3957	0.2814036759555244	No Hit
ACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG	3095	0.22010219284365634	TruSeq Adapter, Index 10 (95% over 23bp)
GAATAATACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCG	2683	0.1908026440709305	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTT	2674	0.19016260538414767	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2452	0.17437498444350413	No Hit
GCCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG	2442	0.17366383034707875	RNA PCR Primer, Index 35 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG	2378	0.1691124441299563	TruSeq Adapter, Index 10 (95% over 23bp)
GGGGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC	2164	0.15389374646645307	RNA PCR Primer, Index 10 (95% over 21bp)
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCCTCTTCTCTC	2103	0.14955570647825825	No Hit
CGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG	1992	0.1416618960079365	TruSeq Adapter, Index 10 (95% over 23bp)
GAATATGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC	1941	0.13803501011616703	RNA PCR Primer, Index 10 (95% over 21bp)
GACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT	1657	0.11783823377768611	RNA PCR Primer, Index 10 (95% over 22bp)
GGACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC	1615	0.1148513865726995	TruSeq Adapter, Index 10 (95% over 21bp)
AGCCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT	1615	0.1148513865726995	RNA PCR Primer, Index 10 (95% over 22bp)
GCTGACTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	1574	0.11193565477735544	RNA PCR Primer, Index 10 (95% over 24bp)
GGCCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT	1490	0.1059619603673822	TruSeq Adapter, Index 10 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG	1443	0.10261953611418291	TruSeq Adapter, Index 10 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.11154096425384E-5	0.0	0.0	0.739173567824544	0.0
2	7.11154096425384E-5	0.0	0.0	4.27325385001049	0.0
3	7.11154096425384E-5	0.0	0.0	5.574096923191802	0.0
4	7.11154096425384E-5	0.0	0.0	7.715524138347918	0.0
5	7.11154096425384E-5	0.0	0.0	15.27260314401226	0.0
6	7.11154096425384E-5	0.0	0.0	18.268695352252404	0.0
7	7.11154096425384E-5	0.0	0.0	21.75747511849605	0.0
8	7.11154096425384E-5	0.0	0.0	25.860336447003018	0.0
9	7.11154096425384E-5	0.0	0.0	27.162672943786824	0.0
10	1.422308192850768E-4	0.0	0.0	33.65188295825881	0.0
11	1.422308192850768E-4	0.0	0.0	39.20969445264247	0.0
12	1.422308192850768E-4	0.0	0.0	44.83563450946368	0.0
13	1.422308192850768E-4	0.0	0.0	46.61885340625033	0.0
14	1.422308192850768E-4	0.0	0.0	47.413212531957484	0.0
15	1.422308192850768E-4	0.0	0.0	49.05818307239904	0.0
16	1.422308192850768E-4	0.0	0.0	51.45676360882258	0.0
17	1.422308192850768E-4	0.0	0.0	54.25757290218431	0.0
18	2.1334622892761517E-4	0.0	0.0	57.03761649593042	0.0
19	2.844616385701536E-4	0.0	0.0	59.26566228003115	0.0
20	2.844616385701536E-4	0.0	0.0	61.2533379795401	0.0
21	2.844616385701536E-4	0.0	0.0	63.2895855038349	0.0
22	2.844616385701536E-4	0.0	0.0	65.40790021085719	0.0
23	2.844616385701536E-4	0.0	0.0	67.00316107995862	0.0
24	2.844616385701536E-4	0.0	0.0	68.09407146387515	0.0
25	2.844616385701536E-4	0.0	0.0	68.96295953888769	0.0
26	2.844616385701536E-4	0.0	0.0	69.66152620780633	0.0
27	2.844616385701536E-4	0.0	0.0	70.42509236113827	0.0
28	3.5557704821269196E-4	0.0	0.0	70.96521389737335	0.0
29	3.5557704821269196E-4	0.0	0.0	71.49566373789705	0.0
30	3.5557704821269196E-4	0.0	0.0	72.06366251471201	0.0
31	3.5557704821269196E-4	0.0	0.0	72.44661899563707	0.0
32	3.5557704821269196E-4	0.0	0.0	72.8117966241515	0.0
33	3.5557704821269196E-4	0.0	0.0	73.14824362717036	0.0
34	3.5557704821269196E-4	0.0	0.0	73.47060977907998	0.0
35	3.5557704821269196E-4	0.0	0.0	73.81246155323166	0.0
36	3.5557704821269196E-4	0.0	0.0	74.06591687319766	0.0
37	3.5557704821269196E-4	0.0	0.0	74.34426258653856	0.0
38	4.2669245785523035E-4	0.0	0.0	74.60234040813134	0.0
39	4.2669245785523035E-4	0.0	0.0	74.97263834614003	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTA	20	7.033824E-4	45.000004	41
GTTTCGG	20	7.033824E-4	45.000004	2
TGCCACG	20	7.033824E-4	45.000004	1
TTCACGG	40	6.8175723E-9	45.000004	2
TAACGGA	20	7.033824E-4	45.000004	3
GTTACCG	20	7.033824E-4	45.000004	38
ACGATAG	20	7.033824E-4	45.000004	1
ATAAGCG	20	7.033824E-4	45.000004	1
CCGGTAT	20	7.033824E-4	45.000004	42
AAATGCG	20	7.033824E-4	45.000004	1
CCGATAT	35	1.2121927E-7	45.0	11
GCACGCG	25	3.891252E-5	45.0	1
TCCTCGC	25	3.891252E-5	45.0	13
ACGATTG	25	3.891252E-5	45.0	1
GTATGCG	25	3.891252E-5	45.0	1
TCGTCCC	75	0.0	45.0	38
GAACACG	35	1.2121927E-7	45.0	1
GCGAGAC	30	2.1658507E-6	44.999996	21
TTGCACG	30	2.1658507E-6	44.999996	1
GCGTAAG	30	2.1658507E-6	44.999996	1
>>END_MODULE