FastQCFastQC Report
Sat 14 Jan 2017
SRR2935230.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935230.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences994529
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT609966.133154488204969No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCG46160.4641393061439134No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCC44320.44563808596833276No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGC42520.4275390662313517No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTC40080.40300483947677745No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30520.30687893465147825No Hit
GCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC20480.20592662456298408No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18730.18833035537425255No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17290.17385113958466772No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCG17260.17354948925571803No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGAGTCGAT15000.15082516447484187No Hit
CCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC12960.13031294210626337No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12790.12860359024221515No Hit
GAACTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCT12410.12478268607551915No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGT11390.11452657489122993No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCT11300.11362162390438087No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10920.10980071973768488No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGC10850.1090968689701356No Hit
CTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGCT10650.10708586677713773Illumina Single End Adapter 2 (95% over 21bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA10450.10507486458413984No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG10020.10075120986919436No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA406.8139343E-945.019
TGTTCGT207.032828E-445.038
GCGTATC207.032828E-445.032
TGCGACG302.1652068E-644.9999961
CGTTATT13750.044.6727261
CGTTTTT189700.043.7783361
CACGACG603.6379788E-1241.24999626
CGGTCTA603.6379788E-1241.24999631
CCGATGA7600.040.26315718
CGATGAA7600.040.26315719
CGGGTAT850.039.7058836
TACGGCT4550.039.0659337
CCACCGA10450.038.9712915
ACCACCG11350.038.85462614
CGACCAC15800.038.73417712
GTTACCG356.247441E-638.57142638
TACCGGT356.247441E-638.57142640
GCGACCA16100.038.0124211
CCGTAGG301.13981136E-437.4999961
ACTACCG301.13981136E-437.49999614
Produced by FastQC (version 0.11.5)