##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935230.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	994529
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.011690961248995	33.0	31.0	34.0	30.0	34.0
2	32.13648068583219	33.0	31.0	34.0	30.0	34.0
3	32.08102730036027	33.0	31.0	34.0	30.0	34.0
4	35.80532794921013	37.0	35.0	37.0	35.0	37.0
5	33.330410676812846	37.0	35.0	37.0	30.0	37.0
6	34.350354791061896	37.0	35.0	37.0	29.0	37.0
7	35.5852267756898	37.0	35.0	37.0	32.0	37.0
8	35.529863885316566	37.0	35.0	37.0	33.0	37.0
9	37.61950933557493	39.0	37.0	39.0	35.0	39.0
10	37.21652963362556	39.0	37.0	39.0	34.0	39.0
11	37.03031887456273	39.0	37.0	39.0	33.0	39.0
12	37.208182969023525	39.0	37.0	39.0	34.0	39.0
13	37.15949358942776	39.0	37.0	39.0	34.0	39.0
14	38.17084971881162	40.0	38.0	41.0	33.0	41.0
15	38.29722109661961	40.0	38.0	41.0	33.0	41.0
16	38.35179466863209	40.0	38.0	41.0	33.0	41.0
17	38.234981584247414	40.0	38.0	41.0	33.0	41.0
18	37.97296207551514	39.0	37.0	41.0	33.0	41.0
19	37.5664430097061	39.0	37.0	41.0	33.0	41.0
20	37.0757635021201	39.0	35.0	40.0	33.0	41.0
21	37.021219089639416	38.0	35.0	40.0	33.0	41.0
22	37.027772945786396	38.0	35.0	40.0	33.0	41.0
23	36.79639608296993	38.0	35.0	40.0	33.0	41.0
24	36.54079971524209	38.0	35.0	40.0	32.0	41.0
25	36.35495495857838	38.0	35.0	40.0	31.0	41.0
26	36.541975146023894	38.0	35.0	40.0	32.0	41.0
27	36.434146213936444	38.0	35.0	40.0	32.0	41.0
28	36.42357638641005	38.0	35.0	40.0	32.0	41.0
29	36.41571738983981	38.0	35.0	40.0	32.0	41.0
30	36.07522555903347	38.0	35.0	40.0	31.0	41.0
31	35.7919256250949	38.0	35.0	40.0	30.0	41.0
32	35.30140197017885	38.0	35.0	40.0	25.0	41.0
33	34.78690113611569	38.0	35.0	40.0	22.0	41.0
34	34.326975885067206	38.0	34.0	40.0	18.0	41.0
35	33.96874600941752	38.0	34.0	40.0	15.0	41.0
36	33.684242490666435	38.0	34.0	40.0	15.0	41.0
37	33.573015970373916	38.0	33.0	40.0	12.0	41.0
38	33.44795073849028	38.0	33.0	40.0	11.0	41.0
39	33.31744574567458	37.0	33.0	40.0	10.0	41.0
40	33.19892029292258	37.0	33.0	40.0	10.0	41.0
41	33.0620635496803	37.0	33.0	40.0	10.0	41.0
42	33.05426789967915	37.0	33.0	40.0	10.0	41.0
43	32.94489954541295	37.0	33.0	40.0	10.0	41.0
44	32.88254238941248	37.0	33.0	40.0	10.0	41.0
45	32.86130620625442	37.0	33.0	40.0	10.0	41.0
46	32.74281795704298	37.0	33.0	40.0	10.0	41.0
47	32.631570321227436	36.0	32.0	40.0	10.0	41.0
48	32.57168368142106	36.0	32.0	40.0	10.0	41.0
49	32.54650895046801	36.0	32.0	40.0	10.0	41.0
50	32.43152889458226	36.0	32.0	40.0	10.0	41.0
51	31.548222324336443	35.0	30.0	39.0	9.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	12.0
9	22.0
10	33.0
11	35.0
12	31.0
13	59.0
14	60.0
15	101.0
16	180.0
17	353.0
18	630.0
19	1238.0
20	2288.0
21	3878.0
22	6227.0
23	9072.0
24	13551.0
25	20639.0
26	28266.0
27	30327.0
28	28697.0
29	25815.0
30	24857.0
31	26465.0
32	30649.0
33	39819.0
34	60300.0
35	77983.0
36	93951.0
37	105620.0
38	167424.0
39	195889.0
40	55.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.1018421785589	15.910948800889669	19.288829184468227	29.698379836083213
2	45.32165477326453	21.27901750476859	21.027642230643853	12.371685491323028
3	24.16098474755387	21.810625934487582	42.35381773683824	11.67457158112031
4	21.024122976806105	20.112032932171914	45.36730452304558	13.496539567976399
5	19.739896976357652	28.6117348010968	39.0027842325362	12.64558399000934
6	19.683387814734413	28.894481709432302	40.827969822901096	10.59416065293219
7	58.714225527862936	5.92250200848844	31.83647736767857	3.526795095970052
8	58.94931168422439	8.156926545128398	29.158827947701877	3.734933822945334
9	53.970170804471266	7.8767939396437905	30.61318473367795	7.539850522206995
10	29.056266835858985	24.490588006986222	35.712583544572354	10.74056161258244
11	23.26206676728381	19.24428548589332	42.68482869780569	14.808819049017172
12	19.94220379697324	17.895305214830337	46.14948382601211	16.013007162184312
13	20.772647152571718	17.946284120422835	47.455629750364245	13.825438976641205
14	15.60115391305834	21.761658031088082	47.13316554871703	15.504022507136545
15	14.188223772258024	21.045841800490482	48.69098839752285	16.074946029728647
16	17.343888413510314	21.568802920779582	44.32168393279633	16.76562473291377
17	17.352234072611257	22.246812310148826	44.619714457798615	15.781239159441304
18	17.09180928861803	20.626447293140775	46.206194087854655	16.075549330386547
19	16.483682225455468	22.3162924359169	44.638316228083845	16.561709110543784
20	17.39386181800631	24.136450520799293	45.11391824672785	13.355769414466549
21	16.67915163861486	24.08054465983395	45.60239067940704	13.637913022144152
22	15.483912485206567	20.98088642965665	46.70150392799003	16.833697157146748
23	15.91859060922306	22.15752381277972	45.80509970046122	16.118785877536
24	15.96464255944271	22.85081681881574	44.04657883279422	17.13796178894733
25	15.600651162510093	24.698525633742204	42.78769145997754	16.913131743770165
26	14.626018949673664	22.724425330985824	45.00723458038931	17.642321138951203
27	16.468599709007982	22.321319941399395	44.89532230834898	16.314758041243643
28	13.718353109864069	23.846665104788297	44.573360857250016	17.861620928097622
29	16.91846089958161	21.89810452988299	44.72127006854501	16.46216450199039
30	17.953523728317624	23.405350673534908	43.29979316842445	15.341332429723014
31	16.924091705722006	24.231470374418443	41.59707761161314	17.247360308246414
32	19.927422930854707	23.752047451607748	40.30832685623044	16.012202761307112
33	19.432113090719323	23.455324078030905	39.597638681224986	17.514924150024786
34	17.345095014826114	24.030671805447604	40.403045059520636	18.221188120205646
35	19.498275062868956	24.69410142891761	38.48294016564625	17.324683342567184
36	19.30139794817446	27.847755067976905	36.75579093219001	16.095056051658624
37	20.233799114957936	27.24686761270913	36.003877212228105	16.51545606010483
38	20.165726690724956	26.530045881015035	35.8080055986301	17.496221829629903
39	20.042854456732783	24.47309228790714	36.31276714907258	19.1712861062875
40	20.282063167589882	23.788245491081707	37.15909742199574	18.770593919332672
41	19.155097538633868	25.48673794328773	34.984701300816766	20.37346321726164
42	19.39048534532427	25.786075619715465	37.1039959619076	17.71944307305267
43	19.3127601105649	25.27397391126855	36.943517986906365	18.469747991260185
44	19.598121321751304	24.040123515754694	36.94603173964761	19.415723422846394
45	19.283701128876082	22.9889726694747	36.2376562171641	21.48966998448512
46	19.715262199493427	24.372139977818648	36.999323297762054	18.913274524925868
47	17.2630461253518	25.629116898551977	38.91057978198725	18.19725719410897
48	17.77776213664961	25.774713457325028	37.2430567635534	19.204467642471965
49	19.12302205365555	23.632694471453323	38.20632681399939	19.037956660891737
50	17.971321097725657	23.75747715752884	37.26316678548338	21.008034959262122
51	17.627238622503718	23.006066188115177	36.30723689304183	23.059458296339272
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4783.0
1	7459.5
2	10136.0
3	50697.5
4	91259.0
5	55570.5
6	19882.0
7	18542.5
8	17203.0
9	17093.5
10	16984.0
11	16586.0
12	16188.0
13	15800.5
14	15413.0
15	14311.0
16	13209.0
17	12836.5
18	12464.0
19	12715.5
20	12967.0
21	12221.0
22	11475.0
23	11903.5
24	12332.0
25	13435.0
26	15384.5
27	16231.0
28	17125.5
29	18020.0
30	19025.5
31	20031.0
32	21840.5
33	23650.0
34	23876.0
35	24102.0
36	25817.0
37	27532.0
38	28927.0
39	30322.0
40	31941.0
41	33560.0
42	36815.5
43	40071.0
44	42287.0
45	44503.0
46	57845.5
47	71188.0
48	69860.5
49	68533.0
50	68038.0
51	67543.0
52	59126.5
53	50710.0
54	45253.5
55	39797.0
56	37418.0
57	35039.0
58	32810.0
59	30581.0
60	27544.5
61	24508.0
62	21691.5
63	18875.0
64	16229.5
65	13584.0
66	11508.0
67	9432.0
68	7818.0
69	6204.0
70	5315.0
71	4426.0
72	3776.0
73	3126.0
74	2536.0
75	1516.0
76	1086.0
77	816.5
78	547.0
79	439.0
80	331.0
81	227.0
82	123.0
83	87.5
84	52.0
85	39.0
86	26.0
87	16.0
88	6.0
89	4.5
90	3.0
91	2.5
92	2.0
93	3.0
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	994529.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.59417379616879
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.64550011157564	26.92539077869529
2	8.583114436280043	6.110177339147144
3	3.5846419132291607	3.8277710176952917
4	2.1128713915281616	3.008236460760255
5	1.4603527163546117	2.5990024194816614
6	1.12884569384633	2.410819788949381
7	0.8785223165796978	2.1889193214105407
8	0.7566757184901461	2.154659762502332
9	0.6418818229194722	2.0562527845437595
>10	5.021622044468017	31.426131750165066
>50	0.13471987901618304	3.2849474962735936
>100	0.04246590616202101	2.7690349187496155
>500	0.0026358148651664318	0.646834381305148
>1k	0.005857366367036515	4.23335120464175
>5k	0.0	0.0
>10k+	2.9286831835182575E-4	6.358470575679098
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	60996	6.133154488204969	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCG	4616	0.4641393061439134	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCC	4432	0.44563808596833276	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGC	4252	0.4275390662313517	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTC	4008	0.40300483947677745	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3052	0.30687893465147825	No Hit
GCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC	2048	0.20592662456298408	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1873	0.18833035537425255	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1729	0.17385113958466772	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCG	1726	0.17354948925571803	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGAGTCGAT	1500	0.15082516447484187	No Hit
CCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC	1296	0.13031294210626337	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1279	0.12860359024221515	No Hit
GAACTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCT	1241	0.12478268607551915	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGT	1139	0.11452657489122993	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCT	1130	0.11362162390438087	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1092	0.10980071973768488	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGC	1085	0.1090968689701356	No Hit
CTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGCT	1065	0.10708586677713773	Illumina Single End Adapter 2 (95% over 21bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1045	0.10507486458413984	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1002	0.10075120986919436	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0110021929978914E-4	0.0	0.0	0.11653757708422781	0.0
2	2.0110021929978914E-4	0.0	0.0	0.5628795138201098	0.0
3	2.0110021929978914E-4	0.0	0.0	0.7663929357514965	0.0
4	2.0110021929978914E-4	0.0	0.0	1.141645944964903	0.0
5	2.0110021929978914E-4	0.0	0.0	2.2552384093374855	0.0
6	2.0110021929978914E-4	0.0	0.0	2.878649089166832	0.0
7	2.0110021929978914E-4	0.0	0.0	3.6004983263434247	0.0
8	2.0110021929978914E-4	0.0	0.0	4.455576458806128	0.0
9	2.0110021929978914E-4	0.0	0.0	4.915693760564046	0.0
10	2.0110021929978914E-4	0.0	0.0	6.448177981738089	0.0
11	2.0110021929978914E-4	0.0	0.0	8.12525326058868	0.0
12	2.0110021929978914E-4	0.0	0.0	9.67935575533745	0.0
13	2.0110021929978914E-4	0.0	0.0	10.106190970801254	0.0
14	2.0110021929978914E-4	0.0	0.0	10.34781288428995	0.0
15	2.0110021929978914E-4	0.0	0.0	10.734428055893796	0.0
16	2.0110021929978914E-4	0.0	0.0	11.327070402170273	0.0
17	2.0110021929978914E-4	0.0	0.0	12.034440423557282	0.0
18	2.0110021929978914E-4	0.0	0.0	12.729241681238053	0.0
19	2.0110021929978914E-4	0.0	0.0	13.406949420278343	0.0
20	2.0110021929978914E-4	0.0	0.0	13.941976553725432	0.0
21	2.0110021929978914E-4	0.0	0.0	14.479718540133067	0.0
22	2.0110021929978914E-4	0.0	0.0	15.102224268975565	0.0
23	2.0110021929978914E-4	0.0	0.0	15.599243460974995	0.0
24	2.0110021929978914E-4	0.0	0.0	15.972988218543652	0.0
25	2.0110021929978914E-4	0.0	0.0	16.274537997383685	0.0
26	2.0110021929978914E-4	0.0	0.0	16.543006790148905	0.0
27	2.0110021929978914E-4	0.0	0.0	16.87170509859441	0.0
28	2.0110021929978914E-4	0.0	0.0	17.140676641907877	0.0
29	2.0110021929978914E-4	0.0	0.0	17.401403076230054	0.0
30	2.0110021929978914E-4	0.0	0.0	17.702852304960437	0.0
31	2.0110021929978914E-4	0.0	0.0	17.973633750247604	0.0
32	4.022004385995783E-4	0.0	0.0	18.22460682393374	0.0
33	4.022004385995783E-4	0.0	0.0	18.461000131720645	0.0
34	4.022004385995783E-4	0.0	0.0	18.71277760628398	0.0
35	4.022004385995783E-4	0.0	0.0	18.966666633149963	0.0
36	4.022004385995783E-4	0.0	0.0	19.22900186922654	0.0
37	4.022004385995783E-4	0.0	0.0	19.48460024795657	0.0
38	4.022004385995783E-4	0.0	0.0	19.797512189187042	0.0
39	4.022004385995783E-4	0.0	0.0	20.326003565506888	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGAA	40	6.8139343E-9	45.0	19
TGTTCGT	20	7.032828E-4	45.0	38
GCGTATC	20	7.032828E-4	45.0	32
TGCGACG	30	2.1652068E-6	44.999996	1
CGTTATT	1375	0.0	44.672726	1
CGTTTTT	18970	0.0	43.778336	1
CACGACG	60	3.6379788E-12	41.249996	26
CGGTCTA	60	3.6379788E-12	41.249996	31
CCGATGA	760	0.0	40.263157	18
CGATGAA	760	0.0	40.263157	19
CGGGTAT	85	0.0	39.705883	6
TACGGCT	455	0.0	39.065933	7
CCACCGA	1045	0.0	38.97129	15
ACCACCG	1135	0.0	38.854626	14
CGACCAC	1580	0.0	38.734177	12
GTTACCG	35	6.247441E-6	38.571426	38
TACCGGT	35	6.247441E-6	38.571426	40
GCGACCA	1610	0.0	38.01242	11
CCGTAGG	30	1.13981136E-4	37.499996	1
ACTACCG	30	1.13981136E-4	37.499996	14
>>END_MODULE