SRR2935228.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935228.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	513894
Sequences flagged as poor quality	0
Sequence length	51
%GC	41

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9537	1.8558301906618873	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGC	5657	1.100810673018171	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTC	4946	0.9624552923365519	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCG	4869	0.9474716575791895	No Hit
GCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC	3546	0.690025569475417	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCC	3276	0.6374855514950554	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2785	0.5419405558344718	No Hit
CCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC	1947	0.3788719074361639	No Hit
CTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGCT	1860	0.3619423460869362	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCACAAGT	1841	0.35824508556239226	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCT	1234	0.24012734143617165	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCG	1140	0.22183563147263832	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1111	0.2161924443562291	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCT	1009	0.19634399311920359	No Hit
TCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC	982	0.1910899913211674	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTC	955	0.1858359895231312	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCACAAGTCGTA	938	0.1825279143169603	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGC	760	0.1478904209817589	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	710	0.13816078802243265	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	656	0.1276527844263603	No Hit
ACTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC	616	0.1198690780588993	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAG	30	2.1631495E-6	45.000004	6
CGGCGAG	80	0.0	45.000004	31
CGCGGGA	20	7.02964E-4	45.000004	4
ATGGACG	20	7.02964E-4	45.000004	1
TTGTACG	20	7.02964E-4	45.000004	1
CCGTCGA	20	7.02964E-4	45.000004	41
TGACCGG	40	6.8030204E-9	45.000004	2
TACTGGC	20	7.02964E-4	45.000004	24
CGGGCCG	20	7.02964E-4	45.000004	6
GTGTAAT	20	7.02964E-4	45.000004	9
GCGATTC	20	7.02964E-4	45.000004	9
ACGTCAG	20	7.02964E-4	45.000004	1
CGTCGAA	20	7.02964E-4	45.000004	42
CAGTCGA	20	7.02964E-4	45.000004	41
AGCTCGG	20	7.02964E-4	45.000004	2
TCGACTG	20	7.02964E-4	45.000004	1
CGTCAGG	20	7.02964E-4	45.000004	2
CGGCGAT	25	3.887785E-5	45.0	31
TCGCAGG	25	3.887785E-5	45.0	2
ACTGCGG	35	1.2101737E-7	45.0	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content